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1. 发明授权

US07776529B2 Methine-substituted cyanine dye compounds 有权
标题翻译：甲基取代的花青染料化合物
公开(公告)号：US07776529B2
公开(公告)日：2010-08-17
申请号：US11005861
申请日：2004-12-06
申请人： Jason Alfred Dallwig , David Carl Hagen , Ching-Ying Cheung , Gerald Alan Thomas , Stephen Yue
发明人： Jason Alfred Dallwig , David Carl Hagen , Ching-Ying Cheung , Gerald Alan Thomas , Stephen Yue
IPC分类号： A61K31/4709 , C07D215/12 , C12Q1/68
CPC分类号： C09B23/164 , C07D277/64 , C07D417/06 , C09B23/0008 , C09B23/04 , C09B23/06 , C09B23/107 , C12Q1/6806 , C12Q1/6876 , C12Q2600/16 , G01N33/5308 , Y10T436/143333
摘要： Cyanine dye compounds having a substituted methine moiety that are nucleic acid stains, particularly for fluorescent staining of RNA, including compounds having the formula where R1 is a C1-C6 alkyl, sulfoalkyl, carboxyalkyl or C1-C6 alkoxy; each R2 is independently selected from the group consisting of H, C1-C6 alkyl, C1-C6 alkoxy, fused benzo, trifluoromethyl, amino, sulfo, carboxy and halogen, that is optionally further substituted; at least one of R3, R4, and R5 is an alkyl, aryl, heteroaryl, cyclic, or heterocyclic moiety that is optionally substituted by alkyl, amino, aminoalkyl, carboxy, nitro, or halogen; and the remaining R3, R4 or R5 are hydrogen; X is S, O, or Se; and D is a substituted or unsubstituted pyridinium, quinolinium or benzazolium moiety.
摘要翻译：具有作为核酸染色的取代的次甲基部分的花青染料化合物，特别是用于RNA的荧光染色，包括具有下式的化合物，其中R 1是C 1 -C 6烷基，磺基烷基，羧基烷基或C 1 -C 6烷氧基; 每个R 2独立地选自H，C 1 -C 6烷基，C 1 -C 6烷氧基，稠合的苯并，三氟甲基，氨基，磺基，羧基和卤素，其任选进一步被取代; R 3，R 4和R 5中的至少一个是任选被烷基，氨基，氨基烷基，羧基，硝基或卤素取代的烷基，芳基，杂芳基，环状或杂环部分; 剩余的R3，R4或R5是氢; X是S，O或Se; 并且D是取代或未取代的吡啶鎓，喹啉鎓或苯并咪唑鎓部分。

2. 发明申请

US20100120051A1 CYANINE DYE COMPOUNDS 有权
标题翻译：氰基染料化合物
公开(公告)号：US20100120051A1
公开(公告)日：2010-05-13
申请号：US12686149
申请日：2010-01-12
申请人： Jason Alfred DALLWIG , David Carl Hagen , Shih-Jung Huang , Gerald Alan Thomas , Stephen Yue
发明人： Jason Alfred DALLWIG , David Carl Hagen , Shih-Jung Huang , Gerald Alan Thomas , Stephen Yue
IPC分类号： C12Q1/68
CPC分类号： C09B23/02 , C12Q1/6827 , G01N33/582 , Y10T436/143333 , C12Q2527/125 , C12Q2563/107
摘要： Cyanine dye compounds having a negatively charged substituent that are nucleic acid stains, particularly for fluorescent staining of DNA, including compounds having the formula wherein W forms one or two fused 5- or 6-membered aromatic rings, a has a value of 0 or 1, n has a value of 0, or 1, X is O, S, or Se, and D is a pyridinium, or quinolinium moiety, provided that the compound has at least one negatively charged substituent.
摘要翻译：具有带负电荷的取代基的花青染料化合物，其特征在于DNA的荧光染色，包括具有下式的化合物，其中W形成一个或两个稠合的5-或6-元芳环，具有0或1的值，n为0或1，X为O，S或Se，D为吡啶鎓或喹啉鎓部分，条件是该化合物具有至少一个带负电荷的取代基。

3. 发明授权

US07446202B2 Cyanine dye compounds 有权
标题翻译：花青染料化合物
公开(公告)号：US07446202B2
公开(公告)日：2008-11-04
申请号：US11005860
申请日：2004-12-06
申请人： Jason Alfred Dallwig , David Carl Hagen , Shih-Jung Huang , Gerald Alan Thomas , Stephen Yue
发明人： Jason Alfred Dallwig , David Carl Hagen , Shih-Jung Huang , Gerald Alan Thomas , Stephen Yue
IPC分类号： C07D215/38
CPC分类号： C09B23/02 , C12Q1/6827 , G01N33/582 , Y10T436/143333 , C12Q2527/125 , C12Q2563/107
摘要： Cyanine dye compounds having a negatively charged substituent that are nucleic acid stains, particularly for fluorescent staining of DNA, including compounds having the formula wherein W forms one or two fused 5- or 6-membered aromatic rings, a has a value of 0 or 1, n has a value of 0, or 1, X is O, S, or Se, and D is a pyridinium, or quinolinium moiety, provided that the compound has at least one negatively charged substituent.
摘要翻译：具有带负电荷的取代基的花青染料化合物，其特征在于DNA的荧光染色，包括具有下式的化合物，其中W形成一个或两个稠合的5-或6-元芳环，具有0或1的值，n为0或1，X为O，S或Se，D为吡啶鎓或喹啉鎓部分，条件是该化合物具有至少一个带负电荷的取代基。

4. 发明申请

US20090047683A1 CYANINE DYE COMPOUNDS 有权
标题翻译：氰基染料化合物
公开(公告)号：US20090047683A1
公开(公告)日：2009-02-19
申请号：US12240218
申请日：2008-09-29
申请人： Jason Alfred Dallwig , David Carl Hagen , Shih-Jung Huang , Gerald Alan Thomas , Stephen Yue
发明人： Jason Alfred Dallwig , David Carl Hagen , Shih-Jung Huang , Gerald Alan Thomas , Stephen Yue
IPC分类号： C12Q1/68 , G01N33/52
CPC分类号： C09B23/02 , C12Q1/6827 , G01N33/582 , Y10T436/143333 , C12Q2527/125 , C12Q2563/107
摘要： Cyanine dye compounds having a negatively charged substituent that are nucleic acid stains, particularly for fluorescent staining of DNA, including compounds having the formula wherein W forms one or two fused 5- or 6-membered aromatic rings, α has a value of 0 or 1, n has a value of 0, or 1, X is O, S, or Se, and D is a pyridinium, or quinolinium moiety, provided that the compound has at least one negatively charged substituent.
摘要翻译：具有带负电荷的取代基的花青染料化合物，特别是用于DNA的荧光染色的带负电荷的取代基，包括具有下式的化合物，其中W形成一个或两个稠合的5-或6-元芳环，α具有0或1的值，n为0或1，X为O，S或Se，D为吡啶鎓或喹啉鎓部分，条件是该化合物具有至少一个带负电荷的取代基。

5. 发明授权

US07282339B2 Competitive immunoassay 有权
标题翻译：竞争性免疫测定
公开(公告)号：US07282339B2
公开(公告)日：2007-10-16
申请号：US10943463
申请日：2004-09-17
申请人： Joseph Beechem , Kyle Richard Gee , David Carl Hagen , Iain D Johnson , Hee-Chol Kang , Christina Pastula
发明人： Joseph Beechem , Kyle Richard Gee , David Carl Hagen , Iain D Johnson , Hee-Chol Kang , Christina Pastula
IPC分类号： G01N33/53 , G01N33/532 , G01N33/533 , C12Q1/42 , C07K1/13
CPC分类号： G01N33/542 , C07F9/12 , C07F9/65318 , C07F9/65522 , C07F9/6596 , C07K16/46 , Y10S435/968 , Y10S436/80 , Y10S530/807 , Y10T436/13
摘要： The present invention provides ligand-detection reagents, ligand analogs and methods for determining the presence of a ligand in a sample. The ligand-detection reagent comprises a ligand-binding antibody and a ligand analog to form an antibody-ligand analog complex wherein the ligand analog is covalently bonded to a reporter molecule. This complex may additionally comprise a labeling protein non-covalently bonded to the antibody to form a ternary complex wherein the labeling protein comprises a monovalent antibody fragment or a non-antibody protein that is covalently bonded to a label moiety. The reporter molecule is either quenched by the ligand-binding antibody or by the label moiety of the labeling protein, depending on the reporter molecule and the ligand-binding antibody, wherein the amount of quenching is directly related to the amount of ligand present in the sample. Alternatively, the ligand analog is fluorogenic wherein the ligand analog is essentially non-fluorescent in solution but when bound by the ligand-binding antibody the detectable signal increases. In this instance a decrease in signal, as opposed to the relieving of quenching, is measured for the presence of a target ligand.
摘要翻译：本发明提供配体检测试剂，配体类似物和用于测定样品中配体存在的方法。配体检测试剂包含配体结合抗体和配体类似物以形成抗体 - 配体类似物复合物，其中配体类似物共价键合到报告分子。该复合物还可以包含与抗体非共价键合以形成三元复合物的标记蛋白质，其中标记蛋白质包含共价键合到标记部分的单价抗体片段或非抗体蛋白质。取决于报道分子和配体结合抗体，报告分子被配体结合抗体或标记蛋白的标记部分淬灭，其中淬灭量与存在于样品。或者，配体类似物是荧光的，其中配体类似物在溶液中基本上是非荧光的，但当被配体结合抗体结合时，可检测信号增加。在这种情况下，与目标配体的存在相比，测量了与消除淬灭相反的信号降低。

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