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    • 1. 发明申请
    • Methods and systems for the analysis of biological sequence data
    • 用于分析生物序列数据的方法和系统
    • US20050059046A1
    • 2005-03-17
    • US10871081
    • 2004-06-18
    • James LaBrenzJon SorensonCurtis Gehman
    • James LaBrenzJon SorensonCurtis Gehman
    • C12Q1/68G06F19/00
    • G16B30/00G01N27/44721G16B40/00
    • Nucleic acid sequence determination is a method whereby peaks in data traces representing the detection of labeled nucleotides are classified as either noise or specific nucleotides. Embodiments are described herein that formulate this classification as a graph theory problem whereby graph edges encode peak characteristics. The graph can then be traversed to find the shortest path. Various embodiments formulate the graph in such a way as to minimize computational time. In various cases it is desirable that such classification allow for the possibility of mixed bases in the nucleotide sequence. Embodiments are described herein that address the classification of mixed-bases. Embodiments are also described that detail methods and systems for processing the data in order to make the classification step robust and reliable.
    • 核酸序列测定是将代表标记的核苷酸检测的数据踪迹中的峰分类为噪声或特定核苷酸的方法。 本文描述了将该分类制定为图论的问题,其中图形边缘编码峰值特性。 然后可以遍历图形以找到最短路径。 各种实施例以使得计算时间最小化的方式来制定图形。 在各种情况下,希望这种分类允许在核苷酸序列中混合碱基的可能性。 本文描述了解混合基底的分类的实施例。 还描述了用于处理数据以便使分类步骤稳健和可靠的详细方法和系统的实施例。