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1. 发明申请

US20100267119A1 RECOMBINATIONAL CLONING USING NUCLEIC ACIDS HAVING RECOMBINATION SITES 审中-公开
公开(公告)号：US20100267119A1
公开(公告)日：2010-10-21
申请号：US12497832
申请日：2009-07-06
申请人： James L. Hartley , Michael A. Brasch , Gary F. Temple , Donna K. Fox
发明人： James L. Hartley , Michael A. Brasch , Gary F. Temple , Donna K. Fox
IPC分类号： C12N1/21 , C12N15/74
CPC分类号： C12N15/64 , C12N9/00 , C12N15/10 , C12N15/66
摘要： Recombinational cloning is provided by the use of nucleic acids, vectors and methods, in vitro and in vivo, for moving or exchanging segments of DNA molecules using engineered recombination sites and recombination proteins to provide chimeric DNA molecules that have the desired characteristic(s) and/or DNA segment(s).

2. 发明申请

US20100267118A1 RECOMBINATIONAL CLONING USING NUCLEIC ACIDS HAVING RECOMBINATION SITES 审中-公开
公开(公告)号：US20100267118A1
公开(公告)日：2010-10-21
申请号：US12497418
申请日：2009-07-02
申请人： James L. Hartley , Michael A. Brasch , Gary F. Temple , Donna K. Fox
发明人： James L. Hartley , Michael A. Brasch , Gary F. Temple , Donna K. Fox
IPC分类号： C12N1/21 , C12N15/74
CPC分类号： C12N9/00 , C12N15/10 , C12N15/64 , C12N15/66
摘要： Recombinational cloning is provided by the use of nucleic acids, vectors and methods, in vitro and in vivo, for moving or exchanging segments of DNA molecules using engineered recombination sites and recombination proteins to provide chimeric DNA molecules that have the desired characteristic(s) and/or DNA segment(s).

3. 发明授权

US06277608B1 Recombinational cloning using nucleic acids having recombination sites 有权
标题翻译：使用具有重组位点的核酸进行重组克隆
公开(公告)号：US06277608B1
公开(公告)日：2001-08-21
申请号：US09296280
申请日：1999-04-22
申请人： James L. Hartley , Michael A. Brasch , Gary F. Temple , Donna K. Fox
发明人： James L. Hartley , Michael A. Brasch , Gary F. Temple , Donna K. Fox
IPC分类号： C12N1564
CPC分类号： C12N15/64 , C12N9/00 , C12N15/10 , C12N15/66
摘要： Recombinational cloning is provided by the use of nucleic acids, vectors and methods, in vitro and in vivo, for moving or exchanging segments of DNA molecules using engineered recombination sites and recombination proteins to provide chimeric DNA molecules that have the desired characteristic(s) and/or DNA segment(s).
摘要翻译：通过在体外和体内使用核酸，载体和方法，使用工程化重组位点和重组蛋白来移动或交换DNA分子片段来提供重组克隆，以提供具有所需特征的嵌合DNA分子和 /或DNA片段。

4. 发明申请

US20100267120A1 RECOMBINATIONAL CLONING USING NUCLEIC ACIDS HAVING RECOMBINATION SITES 审中-公开
标题翻译：使用具有重组位点的核酸进行重组克隆
公开(公告)号：US20100267120A1
公开(公告)日：2010-10-21
申请号：US12497915
申请日：2009-07-06
申请人： James L. Hartley , Michael A. Brasch , Gary F. Temple , Donna K. Fox
发明人： James L. Hartley , Michael A. Brasch , Gary F. Temple , Donna K. Fox
IPC分类号： C12N1/21 , C12N15/74
CPC分类号： C12N15/64 , C12N9/00 , C12N15/10 , C12N15/66
摘要： Recombinational cloning is provided by the use of nucleic acids, vectors and methods, in vitro and in vivo, for moving or exchanging segments of DNA molecules using engineered recombination sites and recombination proteins to provide chimeric DNA molecules that have the desired characteristic(s) and/or DNA segment(s).
摘要翻译：通过在体外和体内使用核酸，载体和方法，使用工程化重组位点和重组蛋白来移动或交换DNA分子片段来提供重组克隆，以提供具有所需特征的嵌合DNA分子和 /或DNA片段。

5. 发明授权

US07408049B2 Recombinational cloning using nucleic acids having recombination sites 有权
标题翻译：使用具有重组位点的核酸进行重组克隆
公开(公告)号：US07408049B2
公开(公告)日：2008-08-05
申请号：US10893235
申请日：2004-07-19
申请人： James L. Hartley , Michael A. Brasch , Gary F. Temple , Donna K. Fox
发明人： James L. Hartley , Michael A. Brasch , Gary F. Temple , Donna K. Fox
IPC分类号： C12N15/11
CPC分类号： C12N15/64 , C12N9/00 , C12N15/10 , C12N15/66
摘要： Recombinational cloning is provided by the use of nucleic acids, vectors and methods, in vitro and in vivo, for moving or exchanging segments of DNA molecules using engineered recombination sites and recombination proteins to provide chimeric DNA molecules that have the desired characteristic(s) and/or DNA segment(s).
摘要翻译：通过在体外和体内使用核酸，载体和方法，使用工程化重组位点和重组蛋白来移动或交换DNA分子片段来提供重组克隆，以提供具有所需特征的嵌合DNA分子和 /或DNA片段。

6. 发明申请

US20120183999A1 USE OF MULTIPLE RECOMBINATION SITES WITH UNIQUE SPECIFICITY IN COMBINATIONAL CLONING 审中-公开
标题翻译：在组合克隆中使用具有独特特异性的多个重组站点
公开(公告)号：US20120183999A1
公开(公告)日：2012-07-19
申请号：US13303771
申请日：2011-11-23
申请人： David CHEO , Michael A. Brasch , Gary F. Temple , James L. Hartley , Devon R.N. Byrd
发明人： David CHEO , Michael A. Brasch , Gary F. Temple , James L. Hartley , Devon R.N. Byrd
IPC分类号： C12P19/34 , C12N9/16 , C12N9/00 , C12N9/12
CPC分类号： C12N15/1086 , C07K14/47 , C12N9/00 , C12N15/10 , C12N15/102 , C12N15/1093 , C12N15/64 , C12N15/66 , C12P21/00
摘要： The present invention provides compositions and methods for recombinational cloning. The compositions include vectors having multiple recombination sites with unique specificity. The methods permit the simultaneous cloning of two or more different nucleic acid molecules. In some embodiments the molecules are fused together while in other embodiments the molecules are inserted into distinct sites in a vector. The invention also generally provides for linking or joining through recombination a number of molecules and/or compounds (e.g., chemical compounds, drugs, proteins or peptides, lipids, nucleic acids, carbohydrates, etc.) which may be the same or different. Such molecules and/or compounds or combinations of such molecules and/or compounds can also be bound through recombination to various structures or supports according to the invention.
摘要翻译：本发明提供了用于重组克隆的组合物和方法。组合物包括具有独特特异性的多个重组位点的载体。该方法允许同时克隆两种或更多种不同的核酸分子。在一些实施方案中，分子融合在一起，而在其它实施方案中，分子被插入载体中的不同位点。本发明还通常提供通过重组连接或连接许多可以相同或不同的分子和/或化合物（例如，化合物，药物，蛋白质或肽，脂质，核酸，碳水化合物等）。这样的分子和/或化合物或这些分子和/或化合物的组合也可以通过重组与根据本发明的各种结构或支持物结合。

7. 发明申请

US20110275541A1 USE OF MULTIPLE RECOMBINATION SITES WITH UNIQUE SPECIFICITY IN COMBINATIONAL CLONING 审中-公开
标题翻译：在组合克隆中使用具有独特特异性的多个重组站点
公开(公告)号：US20110275541A1
公开(公告)日：2011-11-10
申请号：US11971052
申请日：2008-01-08
申请人： David Cheo , Michael A. Brasch , Gary F. Temple , James L. Hartley , Devon R.N. Byrd
发明人： David Cheo , Michael A. Brasch , Gary F. Temple , James L. Hartley , Devon R.N. Byrd
IPC分类号： C40B40/06
CPC分类号： C12N15/1086 , C07K14/47 , C12N9/00 , C12N15/10 , C12N15/102 , C12N15/1093 , C12N15/64 , C12N15/66 , C12P21/00
摘要： The present invention provides compositions and methods for recombinational cloning. The compositions include vectors having multiple recombination sites with unique specificity. The methods permit the simultaneous cloning of two or more different nucleic acid molecules. In some embodiments the molecules are fused together while in other embodiments the molecules are inserted into distinct sites in a vector. The invention also generally provides for linking or joining through recombination a number of molecules and/or compounds (e.g., chemical compounds, drugs, proteins or peptides, lipids, nucleic acids, carbohydrates, etc.) which may be the same or different. Such molecules and/or compounds or combinations of such molecules and/or compounds can also be bound through recombination to various structures or supports according to the invention.
摘要翻译：本发明提供了用于重组克隆的组合物和方法。组合物包括具有独特特异性的多个重组位点的载体。该方法允许同时克隆两种或更多种不同的核酸分子。在一些实施方案中，分子融合在一起，而在其它实施方案中，分子被插入载体中的不同位点。本发明还通常提供通过重组连接或连接许多可以相同或不同的分子和/或化合物（例如，化合物，药物，蛋白质或肽，脂质，核酸，碳水化合物等）。这样的分子和/或化合物或这些分子和/或化合物的组合也可以通过重组与根据本发明的各种结构或支持物结合。

8. 发明授权

US07351578B2 Use of multiple recombination sites with unique specificity in recombinational cloning 有权
标题翻译：在重组克隆中使用具有独特特异性的多个重组位点
公开(公告)号：US07351578B2
公开(公告)日：2008-04-01
申请号：US10640422
申请日：2003-08-14
申请人： David Cheo , Michael A. Brasch , Gary F. Temple , James L. Hartley , Devon R. N. Byrd
发明人： David Cheo , Michael A. Brasch , Gary F. Temple , James L. Hartley , Devon R. N. Byrd
IPC分类号： C12Q1/68
CPC分类号： C12N15/1086 , C07K14/47 , C12N9/00 , C12N15/10 , C12N15/102 , C12N15/1093 , C12N15/64 , C12N15/66 , C12P21/00
摘要： The present invention provides compositions and methods for recombinational cloning. The compositions include vectors having multiple recombination sites with unique specificity. The methods permit the simultaneous cloning of two or more different nucleic acid molecules. In some embodiments the molecules are fused together while in other embodiments the molecules are inserted into distinct sites in a vector. The invention also generally provides for linking or joining through recombination a number of molecules and/or compounds (e.g., chemical compounds, drugs, proteins or peptides, lipids, nucleic acids, carbohydrates, etc.) which may be the same or different. Such molecules and/or compounds or combinations of such molecules and/or compounds can also be bound through recombination to various structures or supports according to the invention.
摘要翻译：本发明提供了用于重组克隆的组合物和方法。组合物包括具有独特特异性的多个重组位点的载体。该方法允许同时克隆两种或更多种不同的核酸分子。在一些实施方案中，分子融合在一起，而在其它实施方案中，分子被插入载体中的不同位点。本发明还通常提供通过重组连接或连接许多可以相同或不同的分子和/或化合物（例如，化合物，药物，蛋白质或肽，脂质，核酸，碳水化合物等）。这样的分子和/或化合物或这些分子和/或化合物的组合也可以通过重组与根据本发明的各种结构或支持物结合。

9. 发明授权

US07198924B2 Methods and compositions for synthesis of nucleic acid molecules using multiple recognition sites 有权
标题翻译：使用多个识别位点合成核酸分子的方法和组合物
公开(公告)号：US07198924B2
公开(公告)日：2007-04-03
申请号：US10792035
申请日：2004-03-04
申请人： Jonathan D. Chesnut , John Carrino , Louis Leong , Knut Madden , Martin A. G. Gleeson , James Fan , Michael A. Brasch , David Cheo , James L. Hartley , Devon R. N. Byrd , Gary F. Temple
发明人： Jonathan D. Chesnut , John Carrino , Louis Leong , Knut Madden , Martin A. G. Gleeson , James Fan , Michael A. Brasch , David Cheo , James L. Hartley , Devon R. N. Byrd , Gary F. Temple
IPC分类号： C12P19/34 , C12Q1/68
CPC分类号： C12N15/66 , C12N9/90 , C12N15/10 , C12N15/64 , C12N15/902 , C12N2800/108 , C12N2800/30 , C12N2800/70 , C12N2840/20 , C12P19/34 , C12Y599/01
摘要： The present invention provides compositions and methods for recombinational cloning. The compositions include vectors having multiple recombination sites and/or multiple topoisomerase recognition sites. The methods permit the simultaneous cloning of two or more different nucleic acid molecules. In some embodiments the molecules are fused together while in other embodiments the molecules are inserted into distinct sites in a vector. The invention also generally provides for linking or joining through recombination a number of molecules and/or compounds (e.g., chemical compounds, drugs, proteins or peptides, lipids, nucleic acids, carbohydrates, etc.) which may be the same or different. The invention also provides host cells comprising nucleic acid molecules of the invention or prepared according to the methods of the invention, and also provides kits comprising the compositions, host cells and nucleic acid molecules of the invention, which may be used to synthesize nucleic acid molecules according to the methods of the invention.
摘要翻译：本发明提供了用于重组克隆的组合物和方法。组合物包括具有多个重组位点和/或多个拓扑异构酶识别位点的载体。该方法允许同时克隆两种或更多种不同的核酸分子。在一些实施方案中，分子融合在一起，而在其它实施方案中，分子被插入载体中的不同位点。本发明还通常提供通过重组连接或连接许多可以相同或不同的分子和/或化合物（例如，化合物，药物，蛋白质或肽，脂质，核酸，碳水化合物等）。本发明还提供了包含本发明的核酸分子或根据本发明的方法制备的宿主细胞，还提供了包含本发明的组合物，宿主细胞和核酸分子的试剂盒，其可用于合成核酸分子根据本发明的方法。

10. 发明授权

US07670823B1 Compositions for use in recombinational cloning of nucleic acids 有权
标题翻译：用于核酸重组克隆的组合物
公开(公告)号：US07670823B1
公开(公告)日：2010-03-02
申请号：US09517466
申请日：2000-03-02
申请人： James L. Hartley , Michael A. Brasch , Gary F. Temple , David Cheo
发明人： James L. Hartley , Michael A. Brasch , Gary F. Temple , David Cheo
IPC分类号： C07H21/04 , C12N1/20 , C12N15/00 , C12N5/10
CPC分类号： C12N15/11 , C12N15/10 , C12N15/63 , C12N2800/70
摘要： The present invention relates generally to compositions and methods for use in recombinational cloning of nucleic acid molecules. In particular, the invention relates to nucleic acid molecules encoding one or more recombination sites or portions thereof, to nucleic acid molecules comprising one or more of these recombination site nucleotide sequences and optionally comprising one or more additional physical or functional nucleotide sequences. The invention also relates to vectors comprising the nucleic acid molecules of the invention, to host cells comprising the vectors or nucleic acid molecules of the invention, to methods of producing polypeptides using the nucleic acid molecules of the invention, and to polypeptides encoded by these nucleic acid molecules or produced by the methods of the invention. The invention also relates to antibodies that bind to one or more polypeptides of the invention or epitopes thereof. The invention also relates to the use of these compositions in methods for recombinational cloning of nucleic acids, in vitro and in vivo, to provide chimeric DNA molecules that have particular characteristics and/or DNA segments.
摘要翻译：本发明一般涉及用于核酸分子重组克隆的组合物和方法。特别地，本发明涉及编码一个或多个重组位点或其部分的核酸分子与包含这些重组位点核苷酸序列中的一个或多个并且任选地包含一个或多个另外的物理或功能核苷酸序列的核酸分子。本发明还涉及包含本发明的核酸分子的载体，包含本发明的载体或核酸分子的宿主细胞，使用本发明的核酸分子产生多肽的方法，以及由这些核酸编码的多肽酸分子或通过本发明的方法制备。本发明还涉及结合本发明的一种或多种多肽或其表位的抗体。本发明还涉及这些组合物用于在体外和体内重组克隆核酸以提供具有特定特征和/或DNA片段的嵌合DNA分子的方法中的用途。

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