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1. 发明申请

US20110314571A1 METHOD TO TRIGGER RNA INTERFERENCE 有权
标题翻译：触发RNA干扰的方法
公开(公告)号：US20110314571A1
公开(公告)日：2011-12-22
申请号：US13216942
申请日：2011-08-24
申请人： James C. Carrington , Edwards Allen
发明人： James C. Carrington , Edwards Allen
IPC分类号： A01H5/00 , C12N5/10 , C12N15/82
CPC分类号： C12N15/8279 , C12N15/111 , C12N15/113 , C12N15/8218 , C12N15/8245 , C12N15/8246 , C12N15/8247 , C12N15/8251 , C12N15/8261 , C12N15/8271 , C12N15/8274 , C12N15/8281 , C12N15/8283 , C12N15/8285 , C12N2310/14 , C12N2320/11
摘要： A method to generate siRNAs in vivo is described, as are constructs and compositions useful in the method. The method does not depend on the use of DNA or synthetic constructs that contain inverted duplications or dual promoters so as to form perfect or largely double-stranded RNA. Rather, the method depends on constructs that yield single-stranded RNA transcripts, and exploits endogenous or in vivo-produced miRNAs or siRNAs to initiate production of siRNAs. The miRNAs or siRNAs guide cleavage of the transcript and set the register for production of siRNAs (usually 21 nucleotides in length) encoded adjacent to the initiation cleavage site within the construct. The method results in specific formation of siRNAs of predictable size and register (phase) relative to the initiation cleavage site. The method can be used to produce specific siRNAs in vivo for inactivation or suppression of one or more target genes or other entities, such as pathogens.
摘要翻译：描述了在体内产生siRNA的方法，以及在该方法中有用的构建体和组合物。该方法不依赖于使用含有反向重复或双重启动子的DNA或合成构建体，以形成完整或大部分双链RNA。相反，该方法取决于产生单链RNA转录物的构建体，并利用内源或体内产生的miRNA或siRNA来引发siRNA的产生。 miRNA或siRNA指导转录物的切割，并设置用于生产与构建体内的起始切割位点相邻编码的siRNA（通常长度为21个核苷酸）的寄存器。该方法导致相对于起始切割位点具有可预测大小和寄生（相）的siRNA的特异性形成。该方法可用于在体内产生特异性siRNA以灭活或抑制一种或多种靶基因或其他实体，例如病原体。

2. 发明授权

US08030473B2 Method to trigger RNA interference 有权
标题翻译：触发RNA干扰的方法
公开(公告)号：US08030473B2
公开(公告)日：2011-10-04
申请号：US11334776
申请日：2006-01-06
申请人： James C. Carrington , Edwards Allen
发明人： James C. Carrington , Edwards Allen
IPC分类号： C12N15/113 , C12N15/63 , C12N15/82
CPC分类号： C12N15/8279 , C12N15/111 , C12N15/113 , C12N15/8218 , C12N15/8245 , C12N15/8246 , C12N15/8247 , C12N15/8251 , C12N15/8261 , C12N15/8271 , C12N15/8274 , C12N15/8281 , C12N15/8283 , C12N15/8285 , C12N2310/14 , C12N2320/11
摘要： A method to generate siRNAs in vivo is described, as are constructs and compositions useful in the method. The method does not depend on the use of DNA or synthetic constructs that contain inverted duplications or dual promoters so as to form perfect or largely double-stranded RNA. Rather, the method depends on constructs that yield single-stranded RNA transcripts, and exploits endogenous or in vivo-produced miRNAs or siRNAs to initiate production of siRNAs. The miRNAs or siRNAs guide cleavage of the transcript and set the register for production of siRNAs (usually 21 nucleotides in length) encoded adjacent to the initiation cleavage site within the construct. The method results in specific formation of siRNAs of predictable size and register (phase) relative to the initiation cleavage site. The method can be used to produce specific siRNAs in vivo for inactivation or suppression of one or more target genes or other entities, such as pathogens.
摘要翻译：描述了在体内产生siRNA的方法，以及在该方法中有用的构建体和组合物。该方法不依赖于使用含有反向重复或双重启动子的DNA或合成构建体，以形成完整或大部分双链RNA。相反，该方法取决于产生单链RNA转录物的构建体，并利用内源或体内产生的miRNA或siRNA来引发siRNA的产生。 miRNA或siRNA指导转录物的切割，并设置用于生产与构建体内的起始切割位点相邻编码的siRNA（通常长度为21个核苷酸）的寄存器。该方法导致相对于起始切割位点具有可预测大小和寄生（相）的siRNA的特异性形成。该方法可用于在体内产生特异性siRNA以灭活或抑制一种或多种靶基因或其他实体，例如病原体。

3. 发明授权

US08476422B2 Method to trigger RNA interference 有权
公开(公告)号：US08476422B2
公开(公告)日：2013-07-02
申请号：US13216942
申请日：2011-08-24
申请人： James C. Carrington , Edwards Allen
发明人： James C. Carrington , Edwards Allen
IPC分类号： C12N15/113 , C12N15/63 , C12N15/82
CPC分类号： C12N15/8279 , C12N15/111 , C12N15/113 , C12N15/8218 , C12N15/8245 , C12N15/8246 , C12N15/8247 , C12N15/8251 , C12N15/8261 , C12N15/8271 , C12N15/8274 , C12N15/8281 , C12N15/8283 , C12N15/8285 , C12N2310/14 , C12N2320/11
摘要： A method to generate siRNAs in vivo is described, as are constructs and compositions useful in the method. The method does not depend on the use of DNA or synthetic constructs that contain inverted duplications or dual promoters so as to form perfect or largely double -stranded RNA. Rather, the method depends on constructs that yield single-stranded RNA transcripts, and exploits endogenous or in vivo-produced miRNAs or siRNAs to initiate production of siRNAs. The miRNAs or siRNAs guide cleavage of the transcript and set the register for production of siRNAs (usually 21 nucleotides in length) encoded adjacent to the initiation cleavage site within the construct. The method results in specific formation of siRNAs of predictable size and register (phase) relative to the initiation cleavage site. The method can be used to produce specific siRNAs in vivo for inactivation or suppression of one or more target genes or other entities, such as pathogens.

4. 发明授权

US5766885A Potyvirus vectors for the expression of foreign genes 失效
标题翻译： Potyvirus载体用于表达外源基因
公开(公告)号：US5766885A
公开(公告)日：1998-06-16
申请号：US468067
申请日：1995-06-06
申请人： James C. Carrington , Valerian V. Dolja
发明人： James C. Carrington , Valerian V. Dolja
IPC分类号： C12N7/01 , C12N15/62 , C12N15/82 , C12N15/01 , C12P21/00
CPC分类号： C12N15/8203 , C12N15/62 , C12N15/8216 , C12N15/8241 , C07K2319/61
摘要： The present invention provides an expression vector adapted for expressing heterologous proteins in plants susceptible to a polyprotein-producing plant virus. The vector utilizes the unique ability of viral polyprotein proteases to cleave heterologous proteins from viral polyproteins. Also provided is a method for expressing heterologous proteins in plants using these unique expression vectors.
摘要翻译：本发明提供了适于在对产生多聚蛋白的植物病毒易感的植物中表达异源蛋白质的表达载体。该载体利用病毒多蛋白蛋白酶独特的能力从病毒多糖蛋白切割异源蛋白质。还提供了使用这些独特的表达载体在植物中表达异源蛋白质的方法。

5. 发明授权

US5491076A Expression of foreign genes using a replicating polyprotein producing virus vector 失效
标题翻译：使用复制多蛋白产生病毒载体表达外源基因
公开(公告)号：US5491076A
公开(公告)日：1996-02-13
申请号：US146881
申请日：1993-11-01
申请人： James C. Carrington , Valerian V. Dolja
发明人： James C. Carrington , Valerian V. Dolja
IPC分类号： C12N7/01 , C12N15/62 , C12N15/82 , C12N15/01 , C12P21/00
CPC分类号： C12N15/8203 , C12N15/62 , C12N15/8216 , C12N15/8241 , C07K2319/61
摘要： The present invention provides an expression vector adapted for expressing heterologous proteins in plants susceptible to a polyprotein-producing plant virus. The vector utilizes the unique ability of viral polyprotein proteases to cleave heterologous proteins from viral polyproteins. Also provided is a method for expressing heterologous proteins in plants using these unique expression vectors.
摘要翻译：本发明提供了适于在对产生多聚蛋白的植物病毒易感的植物中表达异源蛋白质的表达载体。该载体利用病毒多蛋白蛋白酶独特的能力从病毒多糖蛋白切割异源蛋白质。还提供了使用这些独特的表达载体在植物中表达异源蛋白质的方法。

6. 发明授权

US5179007A Method and vector for the purification of foreign proteins 失效
标题翻译：用于纯化外源蛋白的方法和载体
公开(公告)号：US5179007A
公开(公告)日：1993-01-12
申请号：US377438
申请日：1989-07-07
申请人： Donald L. Jarvis , James C. Carrington
发明人： Donald L. Jarvis , James C. Carrington
IPC分类号： C07K14/01 , C07K14/08 , C12N9/50 , C12N15/62 , C12N15/866
CPC分类号： C12N15/86 , C07K14/005 , C12N15/62 , C12N9/503 , C07K2319/09 , C07K2319/50 , C07K2319/61 , C12N2710/14143 , C12N2770/34022
摘要： The present invention provides an method for isolating and purifying recombinantly produced proteins. This invention involves the use of an expression vector which includes a nuclear targeting signal sequence which effectively directs newly synthesized proteins to the nucleus; a cleavage recognition sequence which cleaves specifically at a pre-determined cleavage site after addition of a viral enzyme; and a cDNA sequence which codes for a desired protein. Specifically the production and isolation of a desired protein is accomplished through the use of lepidopteran cells transfected or infected with recombinant baculovirus expression vector comprising a polyhedrin gene derived nuclear targeting signal sequence and a cleavage recognition sequence derived from a potyvirus polyprotein. The newly synthesized proteins are directed into the nucleus whereupon nuclear protein is extracted from the nucleus. Thereafter the desired protein is bound to an affinity matrix embedded with antibodies directed against the nuclear targeting sequence. Afterwards the desired protein is cleaved from the affinity matrix with the addition of a viral enzyme.
摘要翻译：本发明提供了分离和纯化重组产生的蛋白质的方法。本发明涉及使用表达载体，其包括有效地将新合成的蛋白质引导至细胞核的靶向靶向信号序列; 在添加病毒酶之后在预定切割位点特异性切割的切割识别序列; 和编码所需蛋白质的cDNA序列。具体地，通过使用转染或感染重组杆状病毒表达载体的鳞翅目细胞来实现所需蛋白质的生产和分离，所述重组杆状病毒表达载体包含衍生自多核苷酸基因的靶向信号序列和衍生自病毒多蛋白的切割识别序列。新合成的蛋白质被导入细胞核，从核中提取核蛋白质。此后，所需的蛋白质与嵌入针对核靶向序列的抗体的亲和基质结合。之后，通过加入病毒酶将所需蛋白质从亲和基质上切下。

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