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    • 9. 发明申请
    • DIFFERENTIATION OF ISOBARIC AMINO ACIDS AND OTHER SPECIES
    • ISOBARIC氨基酸和其他物种的差异
    • US20130109578A1
    • 2013-05-02
    • US13386333
    • 2009-07-22
    • James R. HeathSu Seong LeeJaehong LimJunhoe ChaSylvia TanShi Yun Yeo
    • James R. HeathSu Seong LeeJaehong LimJunhoe ChaSylvia TanShi Yun Yeo
    • G01N33/68C07K1/13
    • G01N33/6848C07B2200/11C07K1/047C07K1/13C07K7/06C40B50/16G01N33/6812G01N33/6818
    • Techniques for differentiating isobaric species are described. An isobaric species may be substituted with a tagging species identified using mass spectrometry. The isobaric species may be a subunit of a first polymer having a defined sequence, e.g., the isobaric species may be an amino acid in a protein or a peptide sequence. A tagging species may be substituted for the isobaric species in a second polymer having an otherwise identical sequence as the first polymer. The second polymer may have the same number of sequences as the first polymer, and substantially the same sequence of subunits, with a few exceptions such as the tagging species for the isobaric species. The first polymer and the second polymer may be prepared in the same reaction vessel. A polymer/protein of defined subunit sequence containing an isobaric species or a tagging species may be analyzed by mass spectrometry to determine the sequence.
    • 描述了用于区分等压物种的技术。 同量异位物质可以用用质谱鉴定的标记物种代替。 等压异构物可以是具有确定的序列的第一聚合物的亚单位,例如,同量异位物质可以是蛋白质或肽序列中的氨基酸。 在具有与第一聚合物相同的序列的第二聚合物中,标记物质可以代替同量异位物质。 第二聚合物可以具有与第一聚合物相同数量的序列,以及基本上相同的亚基序列,只有少数例外,例如同量异序物种的标记物种。 第一聚合物和第二聚合物可以在相同的反应容器中制备。 可以通过质谱分析含有等压物种或标记物质的限定亚单位序列的聚合物/蛋白质来确定序列。