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    • 6. 发明授权
    • Bacterial cell component-unresponsive model mouse
    • 细菌细胞成分 - 反应性模型小鼠
    • US07078585B1
    • 2006-07-18
    • US09889324
    • 2000-01-13
    • Shizuo AkiraOsamu TakeuchiKiyoshi Takeda
    • Shizuo AkiraOsamu TakeuchiKiyoshi Takeda
    • A01K67/027
    • C12N15/8509A01K67/0276A01K2217/075A01K2227/105A01K2267/03A01K2267/0337C07K14/705G01N33/5047G01N33/5055G01N2800/52
    • A knockout mouse which is unresponsive to peptidoglycan, a lipoprotein/lipopeptide and the like, and is useful for elucidating the contribution of individual members of the TLR family to a signaling stimulated with bacterial cell components in vivo, in particular, the role of TLR2 and MyD88 in vivo. A bacterial cell component-unresponsive knockout mouse is generated by a process comprising the steps of: a targeting vector is constructed by replacing a whole or a part of a gene fragment of an exon region containing a cytoplasmic region of TLR2 or MyD88 gene and the like with a plasmid having a poly A signal and a marker gene; the targeting vector is introduced into an embryonic stem cell; the targeting embryonic stem cell having a homologously recombined TLR2 or MyD88 gene is microinjected into the blastocyst of a mouse and the blastocyst is put back into the uterus of a recipient mouse.
    • 对肽聚糖,脂蛋白/脂肽等无反应的敲除小鼠,并且可用于阐明TLR家族的各个成员对体内细菌细胞成分刺激的信号传导的贡献,特别是TLR2和 MyD88体内 通过包括以下步骤的方法产生细菌细胞成分 - 无反应的敲除小鼠:通过将包含TLR2或MyD88基因的细胞质区域的外显子区域的全部或部分的全部或部分替换来构建靶向载体等 具有聚A信号和标记基因的质粒; 将靶向载体导入胚胎干细胞; 将具有同源重组的TLR2或MyD88基因的靶向胚胎干细胞显微注射到小鼠的囊胚中,并将囊胚放回受体小鼠的子宫中。
    • 8. 发明授权
    • Curable polyorganosiloxane composition
    • 可固化的聚有机硅氧烷组合物
    • US4921926A
    • 1990-05-01
    • US271985
    • 1988-11-16
    • Tsuneo MotegiYasuji MatsumotoKiyoshi TakedaShuji Chiba
    • Tsuneo MotegiYasuji MatsumotoKiyoshi TakedaShuji Chiba
    • C08K5/14C08L83/04C08L83/06C08L83/07
    • C08L83/04C08G77/16C08G77/18C08G77/20
    • A curable polyorganosiloxane composition is disclosed, comprising: (A) 100 parts by weight of polyorganosiloxane having at least two hydroxyl groups or hydrolyzable groups bonded to a silicon atom in the molecule thereof and having a viscosity at 25.degree. C. of 10 to 1,000,000 cSt, (B) 0 to 2,000 parts by weight of polyorganosiloxane having at least two alkenyl groups bonded to a silicon atom in the molecule thereof and having a viscosity at 25.degree. C. of 10 to 1,000,000 cSt, (C) 0.5 to 20 parts by weight of an organosilicon compound having more than 2 (on the average) hydrolyzable groups bonded to a silicon atom in the molecule thereof, (D) 0 to 10 parts by weight of a catalyst for a condensation reaction, and (E) 0.1 to 5 parts by weight of an organic peroxide, wherein at least 0.01 mol % of all organic groups bonded to a silicon atom as contained in (A) to (C) is an alkenyl group. The composition has both condensation reaction curability and ultraviolet ray curability.
    • 公开了一种可固化聚有机硅氧烷组合物,其包含:(A)100重量份具有至少两个羟基的聚有机硅氧烷或与其分子中的硅原子键合的可水解基团,并且在25℃下的粘度为10至1,000,000cSt ,(B)0〜2,000重量份具有至少两个与分子中的硅原子键合的烯基的聚有机硅氧烷,其在25℃下的粘度为10〜1,000,000cSt,(C)为0.5〜20份, (D)0〜10重量份用于缩合反应的催化剂的(E)0.1〜5(重量)的有机硅化合物, (A)〜(C)中所含的与硅原子键合的全部有机基团的至少0.01摩尔%为烯基的有机过氧化物。 该组合物具有缩合反应固化性和紫外线固化性。