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1. 发明授权

US09169493B2 Construction of fully-deleted adenovirus-based gene delivery vectors and uses thereof 有权
标题翻译：完全缺失的基于腺病毒的基因递送载体的构建及其用途
公开(公告)号：US09169493B2
公开(公告)日：2015-10-27
申请号：US14494991
申请日：2014-09-24
申请人： Isogenis, Inc.
发明人： Miles B. Brennan , Erin K. Spiegel , Uwe D. Staerz , Charles Wall , Janae Wheeler , William J. Maslanik , Xianghua Zhang
IPC分类号： C12N15/861 , C12N15/00 , C12N15/86 , C12N7/00
CPC分类号： C12N15/86 , C12N7/00 , C12N2710/10043 , C12N2710/10052 , C12N2710/10343 , C12N2710/10352
摘要： The embodiments disclosed herein relate to the construction of fully-deleted Adenovirus-based gene delivery vectors packaged without helper Adenovirus, and more particularly to their use in gene therapy for gene and protein expression, vaccine development, and immunosuppressive therapy for allogeneic transplantation. In an embodiment, a method for propagating an adenoviral vector includes (a) providing an Adenovirus packaging cell line; (b) transfecting a fully-deleted Adenoviral vector construct into the cell line; and optionally (c) transfecting a packaging construct into the cell line, wherein the fully-deleted Adenoviral vector construct and optionally the packaging construct can transfect the Adenovirus packaging cell line resulting in the encapsidation of a fully-deleted Adenoviral vector independent of helper Adenovirus. In an embodiment, a target cell is transduced with the encapsidated fully-deleted Adenoviral vector for treating a condition, disease or a disorder.
摘要翻译：本文公开的实施方案涉及不含辅助腺病毒包装的完全缺失的基于腺病毒的基因递送载体的构建，更具体地涉及其用于基因和蛋白质表达的基因治疗，疫苗开发和用于同种异体移植的免疫抑制治疗。在一个实施方案中，用于扩增腺病毒载体的方法包括（a）提供腺病毒包装细胞系; （b）将完全缺失的腺病毒载体构建体转染到细胞系中; 并且任选地（c）将包装构建体转染到细胞系中，其中完全缺失的腺病毒载体构建体和任选的包装构建体可以转染腺病毒包装细胞系，导致不依赖于辅助性腺病毒的完全缺失的腺病毒载体的包囊。在一个实施方案中，用封闭的完全缺失的腺病毒载体转导靶细胞用于治疗病症，疾病或病症。

2. 发明授权

US09719107B2 Construction of fully-deleted adenovirus-based gene delivery vectors and uses thereof 有权
公开(公告)号：US09719107B2
公开(公告)日：2017-08-01
申请号：US14921421
申请日：2015-10-23
申请人： Isogenis, Inc.
发明人： Miles B. Brennan , Erin K. Spiegel , Uwe D. Staerz , Charles Wall , Janae Wheeler , William J. Maslanik , Xianghua Zhang
IPC分类号： C12N5/00 , C12N15/86 , C12N7/00
CPC分类号： C12N15/86 , C12N7/00 , C12N2710/10043 , C12N2710/10052 , C12N2710/10343 , C12N2710/10352
摘要： The embodiments disclosed herein relate to the construction of fully-deleted Adenovirus-based gene delivery vectors packaged without helper Adenovirus, and more particularly to their use in gene therapy for gene and protein expression, vaccine development, and immunosuppressive therapy for allogeneic transplantation. In an embodiment, a method for propagating an adenoviral vector includes (a) providing an Adenovirus packaging cell line; (b) transfecting a fully-deleted Adenoviral vector construct into the cell line; and optionally (c) transfecting a packaging construct into the cell line, wherein the fully-deleted Adenoviral vector construct and optionally the packaging construct can transfect the Adenovirus packaging cell line resulting in the encapsidation of a fully-deleted Adenoviral vector independent of helper Adenovirus. In an embodiment, a target cell is transduced with the encapsidated fully-deleted Adenoviral vector for treating a condition, disease or a disorder.

3. 发明申请

US20160168591A1 CONSTRUCTION OF FULLY-DELETED ADENOVIRUS-BASED GENE DELIVERY VECTORS AND USES THEREOF 有权
公开(公告)号：US20160168591A1
公开(公告)日：2016-06-16
申请号：US14921421
申请日：2015-10-23
申请人： Isogenis, Inc.
发明人： Miles B. Brennan , Erin K. Spiegel , Uwe D. Staerz , Charles Wall , Janae Wheeler , William J. Maslanik , Xianghua Zhang
IPC分类号： C12N15/86
CPC分类号： C12N15/86 , C12N7/00 , C12N2710/10043 , C12N2710/10052 , C12N2710/10343 , C12N2710/10352
摘要： The embodiments disclosed herein relate to the construction of fully-deleted Adenovirus-based gene delivery vectors packaged without helper Adenovirus, and more particularly to their use in gene therapy for gene and protein expression, vaccine development, and immunosuppressive therapy for allogeneic transplantation. In an embodiment, a method for propagating an adenoviral vector includes (a) providing an Adenovirus packaging cell line; (b) transfecting a fully-deleted Adenoviral vector construct into the cell line; and optionally (c) transfecting a packaging construct into the cell line, wherein the fully-deleted Adenoviral vector construct and optionally the packaging construct can transfect the Adenovirus packaging cell line resulting in the encapsidation of a fully-deleted Adenoviral vector independent of helper Adenovirus. In an embodiment, a target cell is transduced with the encapsidated fully-deleted Adenoviral vector for treating a condition, disease or a disorder.

4. 发明申请

US20150093831A1 CONSTRUCTION OF FULLY-DELETED ADENOVIRUS-BASED GENE DELIVERY VECTORS AND USES THEREOF 有权
标题翻译：全基因的基于腺病毒的基因递送载体的构建及其用途
公开(公告)号：US20150093831A1
公开(公告)日：2015-04-02
申请号：US14494991
申请日：2014-09-24
申请人： ISOGENIS, INC.
发明人： Miles B. Brennan , Erin K. Spiegel , Uwe D. Staerz , Charles Wall , Janae Wheeler , William J. Maslanik , Xianghua Zhang
IPC分类号： C12N15/86
CPC分类号： C12N15/86 , C12N7/00 , C12N2710/10043 , C12N2710/10052 , C12N2710/10343 , C12N2710/10352
摘要： The embodiments disclosed herein relate to the construction of fully-deleted Adenovirus-based gene delivery vectors packaged without helper Adenovirus, and more particularly to their use in gene therapy for gene and protein expression, vaccine development, and immunosuppressive therapy for allogeneic transplantation. In an embodiment, a method for propagating an adenoviral vector includes (a) providing an Adenovirus packaging cell line; (b) transfecting a fully-deleted Adenoviral vector construct into the cell line; and optionally (c) transfecting a packaging construct into the cell line, wherein the fully-deleted Adenoviral vector construct and optionally the packaging construct can transfect the Adenovirus packaging cell line resulting in the encapsidation of a fully-deleted Adenoviral vector independent of helper Adenovirus. In an embodiment, a target cell is transduced with the encapsidated fully-deleted Adenoviral vector for treating a condition, disease or a disorder.
摘要翻译：本文公开的实施方案涉及不含辅助腺病毒包装的完全缺失的基于腺病毒的基因递送载体的构建，更具体地涉及其用于基因和蛋白质表达的基因治疗，疫苗开发和用于同种异体移植的免疫抑制治疗。在一个实施方案中，用于扩增腺病毒载体的方法包括（a）提供腺病毒包装细胞系; （b）将完全缺失的腺病毒载体构建体转染到细胞系中; 并且任选地（c）将包装构建体转染到细胞系中，其中完全缺失的腺病毒载体构建体和任选的包装构建体可以转染腺病毒包装细胞系，导致不依赖于辅助性腺病毒的完全缺失的腺病毒载体的包囊。在一个实施方案中，用封闭的完全缺失的腺病毒载体转导靶细胞用于治疗病症，疾病或病症。

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