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1. 发明申请

US20050054060A1 Method for the preparation of an evolved microorganism for the creation or the modification of metabolic pathways 有权
标题翻译：制备用于产生或修饰代谢途径的进化微生物的方法
公开(公告)号：US20050054060A1
公开(公告)日：2005-03-10
申请号：US10781499
申请日：2004-02-18
申请人： Michel Chateau , Benjamin Gonzalez , Isabelle Meynial-Salles , Philippe Soucaille , Olivier Zink
发明人： Michel Chateau , Benjamin Gonzalez , Isabelle Meynial-Salles , Philippe Soucaille , Olivier Zink
IPC分类号： C12N1/21 , C12N9/10 , C12N9/88 , C12N15/01 , C12N15/10 , C12P11/00 , C12P13/12 , C12P13/04 , C12N9/08 , C12P13/08
CPC分类号： C12N15/1058 , C12N9/0036 , C12N15/01 , C12P7/18 , C12P7/20 , C12P7/42 , C12P7/52 , C12P13/12 , C12P33/00
摘要： The present invention is directed to a method for the preparation of evolved microorganisms permitting the creation or modification of metabolic pathways. Strains of evolved microorganisms and evolved genes obtained by the method are also provided. The invention is also directed to the use of evolved microorganisms, genes or proteins in a biotransformation process.
摘要翻译：本发明涉及一种用于制备允许创建或修饰代谢途径的进化微生物的方法。还提供了通过该方法获得的进化微生物和进化基因的菌株。本发明还涉及在生物转化过程中使用进化微生物，基因或蛋白质。

2. 发明授权

US08252579B2 Evolved micro-organisms for the production of 1,2-propanediol 有权
标题翻译：用于生产1,2-丙二醇的进化微生物
公开(公告)号：US08252579B2
公开(公告)日：2012-08-28
申请号：US10585040
申请日：2005-01-12
申请人： Isabelle Meynial-Salles , Benjamin Gonzalez , Philippe Soucaille
发明人： Isabelle Meynial-Salles , Benjamin Gonzalez , Philippe Soucaille
IPC分类号： C12Q1/68 , C12P7/02 , C12N1/20 , C12N9/00 , C12N9/02 , C12N9/04 , C12N15/00 , C07H21/04
CPC分类号： C12P7/18 , C12N9/0051 , C12N15/01 , C12P7/28
摘要： The present invention concerns a new method of preparation of a strain of evolved micro-organisms for the production of 1,2-propanediol by the metabolism of a simple carbon source, which method comprises the growth under selection pressure in an appropriate growth medium containing a simple carbon source of an initial bacterial strain that has undergone the deletion of the gene tpiA and the deletion of at least one gene involved in the conversion of methylglyoxal (propanal) into lactate, in order to cause, in said initial strain, the evolution of one or more genes involved in the biosynthesis pathway from DHAP to methylglyoxal and then to 1,2-propanediol towards evolved genes that possess an improved “1,2-propanediol synthase activity”, the resulting strain or strains of evolved micro-organisms possessing an improved “1,2-propanediol synthase activity” then being selected and isolated.The invention also concerns the initial micro-organisms and the evolved micro-organisms thus obtained, and a method for the preparation of 1,2-propanediol and possibly acetone by culture of said evolved micro-organisms.
摘要翻译：本发明涉及通过简单碳源的代谢来制备用于生产1,2-丙二醇的进化微生物菌株的新方法，该方法包括在选择压力下在合适的生长培养基中生长，所述合适的生长培养基含有已经经历基因tpiA缺失的初始细菌菌株的简单碳源和涉及将甲基乙二醛（丙醛）转化成乳酸盐的至少一个基因的缺失，以便在所述初始菌株中引起涉及从DHAP到甲基乙二醛然后到1,2-丙二醇的生物合成途径中的一个或多个基因转化为具有改进的“1,2-丙二醇合酶活性”的进化基因，所得的产生的菌株或进化微生物菌株具有然后选择和分离改进的“1,2-丙二醇合酶活性”。本发明还涉及由此获得的初始微生物和进化微生物，以及通过培养所述进化微生物制备1,2-丙二醇和可能的丙酮的方法。

3. 发明申请

US20070072279A1 Evolved micro-organisms for the production of 1,2-propanediol 有权
标题翻译：用于生产1,2-丙二醇的进化微生物
公开(公告)号：US20070072279A1
公开(公告)日：2007-03-29
申请号：US10585040
申请日：2005-01-12
申请人： Isabelle Meynial-Salles , Benjamin Gonzalez , Philippe Soucaille
发明人： Isabelle Meynial-Salles , Benjamin Gonzalez , Philippe Soucaille
IPC分类号： C12P7/18 , C12N15/74 , C12N1/21
CPC分类号： C12P7/18 , C12N9/0051 , C12N15/01 , C12P7/28
摘要： The present invention concerns a new method of preparation of a strain of evolved micro-organisms for the production of 1,2-propanediol by the metabolism of a simple carbon source, which method comprises the growth under selection pressure in an appropriate growth medium containing a simple carbon source of an initial bacterial strain that has undergone the deletion of the gene tpiA and the deletion of at least one gene involved in the conversion of methylglyoxal (propanal) into lactate, in order to cause, in said initial strain, the evolution of one or more genes involved in the biosynthesis pathway from DHAP to methylglyoxal and then to 1,2-propanediol towards evolved genes that possess an improved “1,2-propanediol synthase activity”, the resulting strain or strains of evolved micro-organisms possessing an improved “1,2-propanediol synthase activity” then being selected and isolated. The invention also concerns the initial micro-organisms and the evolved micro-organisms thus obtained, and a method for the preparation of 1,2-propanediol and possibly acetone by culture of said evolved micro-organisms.
摘要翻译：本发明涉及通过简单碳源的代谢来制备用于生产1,2-丙二醇的进化微生物菌株的新方法，该方法包括在选择压力下在合适的生长培养基中生长，所述合适的生长培养基含有已经经历基因tpiA缺失的初始细菌菌株的简单碳源和涉及将甲基乙二醛（丙醛）转化成乳酸盐的至少一个基因的缺失，以便在所述初始菌株中引起涉及从DHAP到甲基乙二醛然后到1,2-丙二醇的生物合成途径中的一个或多个基因转化为具有改进的“1,2-丙二醇合酶活性”的进化基因，所得的产生的菌株或进化微生物菌株具有然后选择和分离改进的“1,2-丙二醇合酶活性”。本发明还涉及由此获得的初始微生物和进化微生物，以及通过培养所述进化微生物制备1,2-丙二醇和可能的丙酮的方法。

4. 发明申请

US20120135487A1 MUTANT GLYCEROL DEHYDROGENASE (GLYDH) FOR THE PRODUCTION OF A BIOCHEMICAL BY FERMENTATION 有权
标题翻译： MUTANT GLYCEROL DEHYDROGENASE（GLYDH）for the Production of A BIOCHEMICAL BY FERMENTATION
公开(公告)号：US20120135487A1
公开(公告)日：2012-05-31
申请号：US13387337
申请日：2010-07-30
申请人： Francois Voelker , Laurence Dumon-Seignovert , Isabelle Meynial-Salles , Philippe Soucaille
发明人： Francois Voelker , Laurence Dumon-Seignovert , Isabelle Meynial-Salles , Philippe Soucaille
IPC分类号： C12P7/18 , C12N1/21 , C12N1/19 , C12N9/04 , C12N15/53
CPC分类号： C12P7/18 , C12N9/0006
摘要： The present invention concerns a method for the production of 1,2-propanediol, comprising culturing a microorganism modified for an improved production of 1,2-propanediol in an appropriate culture medium and recovery of the 1,2-propanediol which may be further purified wherein the microorganism expresses a glycerol dehydrogenase (GlyDH) enzyme the inhibition of which activity by NAD+ and/or its substrate and/or its product is reduced.The present invention also relates to a mutant glycerol dehydrogenase (GlyDH) comprising at least one amino acid residue in the protein sequence of the parent enzyme replaced by a different amino acid residue at the same position wherein the mutant enzyme has retained more than 50% of the glycerol dehydrogenase activity of the parent enzyme and the glycerol dehydrogenase activity of the mutant GlyDH is less inhibited by NAD+ and/or by its substrate as compared to the parent enzyme and/or by its product as compared to the parent enzyme.
摘要翻译：本发明涉及一种生产1,2-丙二醇的方法，包括在合适的培养基中培养改良生产1,2-丙二醇的微生物，并回收可进一步纯化的1,2-丙二醇其中所述微生物表达甘油脱氢酶（GlyDH）酶，所述NAD +和/或其底物和/或其产物的活性的抑制被降低。本发明还涉及一种突变体甘油脱氢酶（GlyDH），其包含在相同位置的不同氨基酸残基所代替的母体酶的蛋白质序列中的至少一个氨基酸残基，其中突变酶保留超过50％与亲本酶相比，与亲本酶和/或其产物相比，母体酶的甘油脱氢酶活性和突变体GlyDH的甘油脱氢酶活性被NAD +和/或其底物抑制较少。

5. 发明授权

US08980604B2 Mutant glycerol dehydrogenase (GlyDH) for the production of a biochemical by fermentation 有权
标题翻译：突变型甘油脱氢酶（GlyDH）用于通过发酵生产生化
公开(公告)号：US08980604B2
公开(公告)日：2015-03-17
申请号：US13387337
申请日：2010-07-30
申请人： Francois Voelker , Laurence Dumon-Seignovert , Isabelle Meynial-Salles , Philippe Soucaille
发明人： Francois Voelker , Laurence Dumon-Seignovert , Isabelle Meynial-Salles , Philippe Soucaille
IPC分类号： C12N9/04 , C12N15/09 , C12P7/18 , A61K38/00
CPC分类号： C12P7/18 , C12N9/0006
摘要： The present invention concerns a method for the production of 1,2-propanediol, comprising culturing a microorganism modified for an improved production of 1,2-propanediol in an appropriate culture medium and recovery of the 1,2-propanediol which may be further purified wherein the microorganism expresses a glycerol dehydrogenase (GlyDH) enzyme the inhibition of which activity by NAD+ and/or its substrate and/or its product is reduced.The present invention also relates to a mutant glycerol dehydrogenase (GlyDH) comprising at least one amino acid residue in the protein sequence of the parent enzyme replaced by a different amino acid residue at the same position wherein the mutant enzyme has retained more than 50% of the glycerol dehydrogenase activity of the parent enzyme and the glycerol dehydrogenase activity of the mutant GlyDH is less inhibited by NAD+ and/or by its substrate as compared to the parent enzyme and/or by its product as compared to the parent enzyme.
摘要翻译：本发明涉及一种生产1,2-丙二醇的方法，包括在合适的培养基中培养改良生产1,2-丙二醇的微生物，并回收可进一步纯化的1,2-丙二醇其中所述微生物表达甘油脱氢酶（GlyDH）酶，所述NAD +和/或其底物和/或其产物的活性的抑制被降低。本发明还涉及一种突变体甘油脱氢酶（GlyDH），其包含在相同位置的不同氨基酸残基所代替的母体酶的蛋白质序列中的至少一个氨基酸残基，其中突变酶保留超过50％与亲本酶相比，与亲本酶和/或其产物相比，母体酶的甘油脱氢酶活性和突变体GlyDH的甘油脱氢酶活性被NAD +和/或其底物抑制较少。

6. 发明授权

US07745195B2 Method for the preparation of an evolved microorganism for the creation or the modification of metabolic pathways 有权
标题翻译：制备用于产生或修饰代谢途径的进化微生物的方法
公开(公告)号：US07745195B2
公开(公告)日：2010-06-29
申请号：US10781499
申请日：2004-02-18
申请人： Michel Chateau , Benjamin Gonzalez , Isabelle Meynial-Salles , Philippe Noel Paul Soucaille , Olivier Zink
发明人： Michel Chateau , Benjamin Gonzalez , Isabelle Meynial-Salles , Philippe Noel Paul Soucaille , Olivier Zink
IPC分类号： C12N1/00 , C12N1/36
CPC分类号： C12N15/1058 , C12N9/0036 , C12N15/01 , C12P7/18 , C12P7/20 , C12P7/42 , C12P7/52 , C12P13/12 , C12P33/00
摘要： The present invention is directed to a method for the preparation of evolved microorganisms permitting the creation or modification of metabolic pathways. Strains of evolved microorganisms and evolved genes obtained by the method are also provided. The invention is also directed to the use of evolved microorganisms, genes or proteins in a biotransformation process.
摘要翻译：本发明涉及一种用于制备允许创建或修饰代谢途径的进化微生物的方法。还提供了通过该方法获得的进化微生物和进化基因的菌株。本发明还涉及在生物转化过程中使用进化微生物，基因或蛋白质。

7. 发明申请

US20060270013A1 Method for the production of evolved microorganisms which permit the generation or modification of metabolic pathways 审中-公开
标题翻译：用于生产允许产生或修饰代谢途径的进化微生物的方法
公开(公告)号：US20060270013A1
公开(公告)日：2006-11-30
申请号：US10546139
申请日：2004-02-17
申请人： Michel Chateau , Benjamin Gonzalez , Isabelle Meynial-Salles , Philippe Soucailles , Olivier Zink
发明人： Michel Chateau , Benjamin Gonzalez , Isabelle Meynial-Salles , Philippe Soucailles , Olivier Zink
IPC分类号： C12N15/74 , C12N9/10 , C12N1/21
CPC分类号： C12N15/1058 , C12N9/0036 , C12N15/01 , C12P7/18 , C12P7/20 , C12P7/42 , C12P7/52 , C12P13/12 , C12P33/00
摘要： The invention relates to a method for the preparation of evolved microorganisms which permit a modification of metabolic pathways, comprising the following steps: a) production of a modified microorganism by genetic modification of initial microorganism cells such as to inhibit the production of or the consumption of a metabolite when the microorganism is cultivated in a defined medium which also affects the capacity of the microorganism for growth, b) culture of the modified microorganisms to induce evolution in said cells, c) selection of the cells of modified microorganisms which are capable of developing in the defined medium. The invention also relates to the strains of evolved microorganisms, the genes evolved which code for evolved proteins and the use of said evolved microorganisms, genes or proteins in a biotransformation method.
摘要翻译：本发明涉及一种用于制备能够修饰代谢途径的进化微生物的方法，包括以下步骤：a）通过初始微生物细胞的遗传修饰来产生经修饰的微生物，例如抑制或抑制微生物在限定培养基中培养的代谢物，其也影响微生物生长的能力，b）培养经修饰的微生物以诱导所述细胞中的进化，c）选择能够发育的修饰的微生物细胞在定义的介质中。本发明还涉及进化微生物菌株，进化型编码进化蛋白质的基因以及所述进化微生物，基因或蛋白质在生物转化方法中的应用。

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