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    • 5. 发明授权
    • Method for producing extracellular proteins from genus Tepidimonas
    • 从Tepidimonas属产生胞外蛋白的方法
    • US09580738B2
    • 2017-02-28
    • US14838612
    • 2015-08-28
    • INDUSTRIAL TECHNOLOGY RESEARCH INSTITUTE
    • Yin-Lung HanJo-Shu ChangYung-Chong LouChieh-Lun ChengChih-Hsi Liu
    • C12P21/00
    • C12P21/00
    • The disclosure provides a method for producing extracellular proteins from genus Tepidimonas, including: performing a fermentation culturing to a bacteria of genus Tepidimonas with a culturing medium to form a fermented liquid, wherein the composition of the culturing medium includes a carbon source which is an organic acid, selected from a group consisting of acetate, lactate and butyrate; a nitrogen source selected from a group consisting of (NH4)2SO4, NH4NO3, NH4Cl and urea; phosphate; carbonate; MgCl2; yeast extract; and trace elements, and wherein the gas feeding rate for the fermentation culturing is about 0-0.1 vvm; and after the fermentation culturing is completed, collecting the fermented liquid, wherein the fermented liquid contains extracellular protein secreted from the bacteria of genus Tepidimonas.
    • 本发明提供了从Tepidimonas属产生细胞外蛋白的方法,包括:用培养基对培养培养基进行发酵培养以形成发酵液,其中培养基的组成包括作为有机物的碳源 酸,选自乙酸酯,乳酸和丁酸酯; 选自(NH 4)2 SO 4,NH 4 NO 3,NH 4 Cl和尿素的氮源; 磷酸盐 碳酸盐 MgCl2; 酵母抽提物; 和微量元素,其中发酵培养的气体进料速率为约0-0.1vvm; 在发酵培养完成后,收集发酵液,其中发酵液含有从Tepidimonas属细菌分泌的细胞外蛋白质。