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1. 发明申请

US20200002700A1 COMPOSITIONS AND METHODS FOR PREPARING OLIGONUCLEOTIDE SOLUTIONS 审中-公开
公开(公告)号：US20200002700A1
公开(公告)日：2020-01-02
申请号：US16512986
申请日：2019-07-16
申请人： Illumina, Inc.
发明人： John R. Stuelpnagel , Mark S. Chee
IPC分类号： C12N15/10 , C12Q1/6834 , C12Q1/6837 , C12Q1/6876 , G11C7/10 , H03K19/096 , C12Q1/686 , C12Q1/6806
摘要： The present invention is directed to methods and compositions for generating a pool of oligonucleotides. The invention finds use in preparing a population or subpopulations of oligonucleotides in solution. The pool of oligonucleotides finds use in a variety of nucleic acid detection and/or amplification assays.

2. 发明申请

US20180094316A1 MULTIPLEX NUCLEIC ACID REACTIONS 审中-公开
公开(公告)号：US20180094316A1
公开(公告)日：2018-04-05
申请号：US15827141
申请日：2017-11-30
申请人： Illumina, Inc.
发明人： Arnold Oliphant , John R. Stuelpnagel , Mark S. Chee , Scott L. Butler , Jian-Bing Fan , Min-Jui Richard Shen
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6876 , C12Q1/6834 , C12Q1/6837 , C12Q2600/16 , Y10T436/143333 , C12Q2525/155 , C12Q2563/131 , C12Q2563/179
摘要： A method for detecting nucleic acids by (a) providing a sample having target nucleic acids, each nucleic acid having contiguous first, second, and third domains; (b) contacting the sample with probe sets to form hybridization complexes, wherein each probe set includes (i) a first probe having a sequence that is complementary to the first domain; and (ii) a second probe having a sequence substantially complementary to the third domain; (c) extending the first probes along the second domains of the complexes while the complexes are immobilized on a solid support; (d) ligating the extended first probes to the second probes to form templates; (e) amplifying the templates with primers that are complementary to the first and second priming sequences to produce amplicons; and (f) detecting the amplicons on the surface of a nucleic acid array.

3. 发明申请

US20180073016A1 COMPOSITIONS AND METHODS FOR REPRESENTATIONAL SELECTION OF NUCLEIC ACIDS FROM COMPLEX MIXTURE 审中-公开
公开(公告)号：US20180073016A1
公开(公告)日：2018-03-15
申请号：US15818712
申请日：2017-11-20
申请人： ILLUMINA, INC.
发明人： John R. Stuelpnagel , David L. Barker , Jorge Velarde , Steven M. Barnard , Michael Graige
IPC分类号： C12N15/10 , C12Q1/68
CPC分类号： C12N15/1072 , C12Q1/6837 , C12Q2565/515
摘要： The invention provides a method of selecting a representational sample of nucleic acid sequences from a complex mixture. The method includes: (a) contacting a complex mixture of nucleic acids under conditions sufficient for hybridization with a population of capture probes complementary to one or more nucleic acids comprising a predetermined portion of the sequence collectively present in the complex mixture to form hybridization complexes of the one or more nucleic acids with the population of probes, the population of capture probes being attached to a solid support, and (b) removing unhybridized nucleic acids to select a representational sample of nucleic acids having a complexity of less than 10% but more than 0.001% of the complex mixture, wherein the representational sample comprises a nucleic acid copy having a proportion of each sequence in the copy relative to all other sequences in the copy substantially the same as the proportions of the sequences in the predetermined portion of one or more nucleic acids within the complex mixture. A method of selecting a representational sample of genomic sequences from a complete genome also is provided. The invention further provides a nucleic acid population that includes a representational sample having a complexity of less than 10% but more than 0.001% of a complex mixture, the representational sample comprising a nucleic acid copy having a proportion of each sequence in the copy relative to all other sequences in the copy substantially the same as the proportions of sequences in a predetermined portion of a sequence collectively present in one or more nucleic acids within the complex mixture.

4. 发明申请

US20160348098A1 COMPOSITIONS AND METHODS FOR PREPARING OLIGONUCLEOTIDE SOLUTIONS 有权
标题翻译：用于制备寡核苷酸解决方案的组合物和方法
公开(公告)号：US20160348098A1
公开(公告)日：2016-12-01
申请号：US15237490
申请日：2016-08-15
申请人： ILLUMINA, INC.
发明人： John R. Stuelpnagel , Mark Chee
IPC分类号： C12N15/10 , C12Q1/68
CPC分类号： C12N15/1093 , C12Q1/6806 , C12Q1/6834 , C12Q1/6837 , C12Q1/686 , C12Q1/6876 , G11C7/1048 , H03K19/0966 , Y10S977/792 , C12Q2525/197 , C12Q2523/107
摘要： The present invention is directed to methods and compositions for generating a pool of oligonucleotides. The invention finds use in preparing a population or subpopulations of oligonucleotides in solution. The pool of oligonucleotides finds use in a variety of nucleic acid detection and/or amplification assays.
摘要翻译：本发明涉及用于产生寡核苷酸库的方法和组合物。本发明用于制备溶液中寡核苷酸的群体或亚群。寡核苷酸库可用于各种核酸检测和/或扩增测定。

5. 发明申请

US20150065387A1 COMPOSITIONS AND METHODS FOR REPRESENTATIONAL SELECTION OF NUCLEIC ACIDS FROM COMPLEX MIXTURES USING HYBRIDIZATION 有权
标题翻译：使用混合从复合混合物中代表性选择核酸的组合物和方法
公开(公告)号：US20150065387A1
公开(公告)日：2015-03-05
申请号：US14536354
申请日：2014-11-07
申请人： Illumina, Inc.
发明人： John R. Stuelpnagel , David L. Barker , Jorge Velarde, JR. , Steven M. Barnard , Michael Graige
IPC分类号： C12N15/10 , C12Q1/68
CPC分类号： C12N15/1072 , C12Q1/6837 , C12Q2565/515
摘要： The invention provides a method of selecting a representational sample of nucleic acid sequences from a complex mixture. The method includes: (a) contacting a complex mixture of nucleic acids under conditions sufficient for hybridization with a population of capture probes complementary to one or more nucleic acids comprising a predetermined portion of the sequence collectively present in the complex mixture to form hybridization complexes of the one or more nucleic acids with the population of probes, the population of capture probes being attached to a solid support, and (b) removing unhybridized nucleic acids to select a representational sample of nucleic acids having a complexity of less than 10% but more than 0.001% of the complex mixture, wherein the representational sample comprises a nucleic acid copy having a proportion of each sequence in the copy relative to all other sequences in the copy substantially the same as the proportions of the sequences in the predetermined portion of one or more nucleic acids within the complex mixture. A method of selecting a representational sample of genomic sequences from a complete genome also is provided. The invention further provides a nucleic acid population that includes a representational sample having a complexity of less than 10% but more than 0.001% of a complex mixture, the representational sample comprising a nucleic acid copy having a proportion of each sequence in the copy relative to all other sequences in the copy substantially the same as the proportions of sequences in a predetermined portion of a sequence collectively present in one or more nucleic acids within the complex mixture.
摘要翻译：本发明提供了从复杂混合物中选择核酸序列的表示样品的方法。该方法包括：（a）在足以与包含与复合物混合物中共同存在的序列的预定部分的一个或多个核酸互补的捕获探针的杂交群的条件下使核酸的复杂混合物接触，以形成杂合复合物具有探针群的一个或多个核酸，捕获探针的群体连接到固体支持物上，和（b）除去未杂交的核酸以选择复杂度小于10％但更多的核酸的代表性样品超过0.001％的复合混合物，其中代表性样品包含核酸拷贝，其拷贝中的每个序列的比例相对于拷贝中的所有其它序列基本上与在一个或更多个预定部分中的序列的比例相同复杂混合物中更多的核酸。还提供了从完整基因组中选择基因组序列的表示样品的方法。本发明还提供了一种核酸群体，其包括具有小于复合物混合物的10％但大于0.001％的复杂性的代表性样品，所述代表性样品包含核酸拷贝，所述核酸拷贝具有拷贝中每个序列的比例相对于拷贝中的所有其它序列基本上与复合物混合物中一个或多个核酸中共存的序列的预定部分中的序列比例相同。

6. 发明申请

US20170175109A1 COMPOSITIONS AND METHODS FOR REPRESENTATIONAL SELECTION OF NUCLEIC ACIDS FROM COMPLEX MIXTURE 审中-公开
公开(公告)号：US20170175109A1
公开(公告)日：2017-06-22
申请号：US15451035
申请日：2017-03-06
申请人： Illumina, Inc.
发明人： John R. Stuelpnagel , David L. Barker , Jorge Velarde, Jr. , Steven M. Barnard , Michael Graige
IPC分类号： C12N15/10 , C12Q1/68
CPC分类号： C12N15/1072 , C12Q1/6837 , C12Q2565/515
摘要： The invention provides a method of selecting a representational sample of nucleic acid sequences from a complex mixture. The method includes: (a) contacting a complex mixture of nucleic acids under conditions sufficient for hybridization with a population of capture probes complementary to one or more nucleic acids comprising a predetermined portion of the sequence collectively present in the complex mixture to form hybridization complexes of the one or more nucleic acids with the population of probes, the population of capture probes being attached to a solid support, and (b) removing unhybridized nucleic acids to select a representational sample of nucleic acids having a complexity of less than 10% but more than 0.001% of the complex mixture, wherein the representational sample comprises a nucleic acid copy having a proportion of each sequence in the copy relative to all other sequences in the copy substantially the same as the proportions of the sequences in the predetermined portion of one or more nucleic acids within the complex mixture. A method of selecting a representational sample of genomic sequences from a complete genome also is provided. The invention further provides a nucleic acid population that includes a representational sample having a complexity of less than 10% but more than 0.001% of a complex mixture, the representational sample comprising a nucleic acid copy having a proportion of each sequence in the copy relative to all other sequences in the copy substantially the same as the proportions of sequences in a predetermined portion of a sequence collectively present in one or more nucleic acids within the complex mixture.

7. 发明申请

US20140045720A1 COMPOSITIONS AND METHODS FOR REPRESENTATIONAL SELECTION OF NUCLEIC ACIDS FROM COMPLEX MIXTURES USING HYBRIDIZATION 有权
标题翻译：使用混合从复合混合物中代表性选择核酸的组合物和方法
公开(公告)号：US20140045720A1
公开(公告)日：2014-02-13
申请号：US14046859
申请日：2013-10-04
申请人： Illumina, Inc.
发明人： John R. Stuelpnagel , David L. Barker , Jorge Velarde, JR. , Steven M. Barnard , Michael Graige
IPC分类号： C12N15/10
CPC分类号： C12N15/1072 , C12Q1/6837 , C12Q2565/515
摘要： The invention provides a method of selecting a representational sample of nucleic acid sequences from a complex mixture. The method includes: (a) contacting a complex mixture of nucleic acids under conditions sufficient for hybridization with a population of capture probes complementary to one or more nucleic acids comprising a predetermined portion of the sequence collectively present in the complex mixture to form hybridization complexes of the one or more nucleic acids with the population of probes, the population of capture probes being attached to a solid support, and (b) removing unhybridized nucleic acids to select a representational sample of nucleic acids having a complexity of less than 10% but more than 0.001% of the complex mixture, wherein the representational sample comprises a nucleic acid copy having a proportion of each sequence in the copy relative to all other sequences in the copy substantially the same as the proportions of the sequences in the predetermined portion of one or more nucleic acids within the complex mixture. A method of selecting a representational sample of genomic sequences from a complete genome also is provided. The invention further provides a nucleic acid population that includes a representational sample having a complexity of less than 10% but more than 0.001% of a complex mixture, the representational sample comprising a nucleic acid copy having a proportion of each sequence in the copy relative to all other sequences in the copy substantially the same as the proportions of sequences in a predetermined portion of a sequence collectively present in one or more nucleic acids within the complex mixture.
摘要翻译：本发明提供了从复杂混合物中选择核酸序列的表示样品的方法。该方法包括：（a）在足以与包含与复合物混合物中共同存在的序列的预定部分的一个或多个核酸互补的捕获探针的杂交群的条件下使核酸的复杂混合物接触，以形成杂合复合物具有探针群的一个或多个核酸，捕获探针的群体连接到固体支持物上，和（b）除去未杂交的核酸以选择复杂度小于10％但更多的核酸的代表性样品超过0.001％的复合混合物，其中代表性样品包含核酸拷贝，其拷贝中的每个序列的比例相对于拷贝中的所有其它序列基本上与在一个或更多个预定部分中的序列的比例相同复杂混合物中更多的核酸。还提供了从完整基因组中选择基因组序列的表示样品的方法。本发明还提供了一种核酸群体，其包括具有小于复合物混合物的10％但大于0.001％的复杂性的代表性样品，所述代表性样品包含核酸拷贝，所述核酸拷贝具有拷贝中每个序列的比例相对于拷贝中的所有其它序列基本上与复合物混合物中一个或多个核酸中共存的序列的预定部分中的序列比例相同。

8. 发明申请

US20130345067A1 USE OF MICROFLUIDIC SYSTEMS IN THE DETECTION OF TARGET ANALYTES USING MICROSPHERE ARRAYS 有权
公开(公告)号：US20130345067A1
公开(公告)日：2013-12-26
申请号：US13912668
申请日：2013-06-07
申请人： Illumina, Inc
发明人： Mark S. Chee , Todd A. Dickinson , Kevin Gunderson , Don O'Neil , John R. Stuelpnagel
IPC分类号： C12Q1/68
CPC分类号： B01L3/502761 , B01L2200/0668 , B01L2300/0816 , B01L2300/0867 , B01L2400/0415 , C12Q1/68 , C12Q1/6816 , C12Q1/6869 , C12Q1/6874 , C12Q2565/629 , C12Q2563/149
摘要： The invention relates generally to methods and apparatus for conducting analyses, particularly microfluidic devices for the detection of target analytes.

9. 发明申请

US20170144126A1 COMPOSITE ARRAYS UTILIZING MICROSPHERES WITH A HYBRIDIZATION CHAMBER 审中-公开
公开(公告)号：US20170144126A1
公开(公告)日：2017-05-25
申请号：US15354328
申请日：2016-11-17
申请人： Illumina, Inc.
发明人： John R. Stuelpnagel , Mark S. Chee , Steven R. Auger , Gan G, Wang , Laura S. Casas , Shawn Christopher Baker , Robert C. Kain
IPC分类号： B01J19/00
CPC分类号： B01J19/0046 , B01J2219/00315 , B01J2219/00317 , B01J2219/00382 , B01J2219/005 , B01J2219/00511 , B01J2219/00524 , B01J2219/00585 , B01J2219/00596 , B01J2219/00605 , B01J2219/00612 , B01J2219/00621 , B01J2219/00626 , B01J2219/00637 , B01J2219/00648 , B01J2219/00659 , B01J2219/00704 , B01J2219/0072 , B01J2219/00722 , C40B40/06 , G01N33/54393
摘要： The invention relates to sensor compositions comprising a composite array of individual arrays, to allow for simultaneous processing of a number of samples. The invention further provides methods of making and using the composite arrays. The invention further provides a hybridization chamber for use with a composite array.

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