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1. 发明授权

US07919745B2 Methods and systems for background correction in tandem mass spectrometry based quantitation 有权
标题翻译：基于串联质谱法定量的背景校正方法和系统
公开(公告)号：US07919745B2
公开(公告)日：2011-04-05
申请号：US12208277
申请日：2008-09-10
申请人： Ignat V. Shilov , Wilfred H. Tang
发明人： Ignat V. Shilov , Wilfred H. Tang
IPC分类号： B01D59/44
CPC分类号： H01J49/004 , Y10T436/105831
摘要： A corrected reporter ion intensity is calculated in tandem mass spectrometry based quantitation using isobaric labels. An analyte in each of two or more samples of a mixture of samples is labeled with a different isobaric label. The analyte is eluted from the mixture of samples and intensities of the eluting analyte are measured. An analyte intensity is selected at each of at least two times from the measured intensities. Tandem mass spectrometry is performed on the eluting analyte at each of the at least two times. A plurality of reporter ion intensities is produced. A system of linear equations is created expressing each reporter ion intensity of the plurality of reporter ion intensities as a sum of a background noise intensity and the product of a fragmentation efficiency and an analyte intensity. A corrected reporter ion intensity is calculated from a solution of the system of linear equations.
摘要翻译：在使用等压标记的基于串联质谱法的定量中计算校正的报告离子强度。样品混合物的两个或更多个样品中的每一个中的分析物用不同的同量异序标记物标记。分析物从样品混合物中洗脱，并测量洗脱分析物的强度。从测量的强度中选择至少两次的分析物强度。在洗脱分析物中进行至少两次的串联质谱分析。产生多个报道离子强度。产生线性方程式的系统，表示多个报告离子强度的每个报告离子强度作为背景噪声强度和碎裂效率与分析物强度的乘积之和。从线性方程组的解求出校正的报道离子强度。

2. 发明申请

US20090283673A1 METHODS AND SYSTEMS FOR ANALYSIS AND CORRECTION OF MASS SPECTROMETER DATA 有权
标题翻译：用于分析和校正大量光谱仪数据的方法和系统
公开(公告)号：US20090283673A1
公开(公告)日：2009-11-19
申请号：US12476141
申请日：2009-06-01
申请人： Ignat V. Shilov , Wilfred H. Tang
发明人： Ignat V. Shilov , Wilfred H. Tang
IPC分类号： B01D59/44 , H01J49/00
CPC分类号： H01J49/004 , H01J49/0036 , Y10T436/24
摘要： A background component of reporter ion signals is determined by fitting a distribution function. A plurality of samples that include a protein labeled with a plurality of isobaric reporter ions is analyzed at a plurality of different times using a mass spectrometer, producing a plurality of mass spectra for the plurality of isobaric reporter ions. A cumulative distribution is calculated for an inverse coefficient of differential expression of the plurality of mass spectra using a processor. A Pearson Type IV distribution shifted by a constant value is fitted to the cumulative distribution and the constant value is solved for using the processor. A background component for each spectrum of the plurality of mass spectra is calculated from the constant value, a calculated coefficient of differential expression for each spectrum, and an average reporter ion signal value for each spectrum using the processor.
摘要翻译：通过拟合分布函数来确定报告离子信号的背景分量。使用质谱仪在多个不同时间分析包括用多个等压报告基因离子标记的蛋白质的多个样品，产生多个等压报道离子的多个质谱。使用处理器计算多个质谱的差分表达的逆系数的累积分布。将一个固定值偏移的Pearson IV型分布拟合到累积分布中，并解决使用该处理器的常数值。从恒定值，每个光谱的差分表达式的计算系数和使用该处理器的每个光谱的平均报告离子信号值计算多个质谱中的每个光谱的背景分量。

3. 发明授权

US07982180B2 Methods and systems for analysis and correction of mass spectrometer data 有权
标题翻译：分析和校正质谱仪数据的方法和系统
公开(公告)号：US07982180B2
公开(公告)日：2011-07-19
申请号：US12476141
申请日：2009-06-01
申请人： Ignat V. Shilov , Wilfred H. Tang
发明人： Ignat V. Shilov , Wilfred H. Tang
IPC分类号： B01D59/44 , H01J49/00
CPC分类号： H01J49/004 , H01J49/0036 , Y10T436/24
摘要： A background component of reporter ion signals is determined by fitting a distribution function. A plurality of samples that include a protein labeled with a plurality of isobaric reporter ions is analyzed at a plurality of different times using a mass spectrometer, producing a plurality of mass spectra for the plurality of isobaric reporter ions. A cumulative distribution is calculated for an inverse coefficient of differential expression of the plurality of mass spectra using a processor. A Pearson Type IV distribution shifted by a constant value is fitted to the cumulative distribution and the constant value is solved for using the processor. A background component for each spectrum of the plurality of mass spectra is calculated from the constant value, a calculated coefficient of differential expression for each spectrum, and an average reporter ion signal value for each spectrum using the processor.
摘要翻译：通过拟合分布函数来确定报告离子信号的背景分量。使用质谱仪在多个不同时间分析包括用多个等压报告基因离子标记的蛋白质的多个样品，产生多个等压报道离子的多个质谱。使用处理器计算多个质谱的差分表达的逆系数的累积分布。将一个固定值偏移的Pearson IV型分布拟合到累积分布中，并解决使用该处理器的常数值。从恒定值，每个光谱的差分表达式的计算系数和使用该处理器的每个光谱的平均报告离子信号值计算多个质谱中的每个光谱的背景分量。

4. 发明申请

US20090065686A1 METHODS AND SYSTEMS FOR BACKGROUND CORRECTION IN TANDEM MASS SPECTROMETRY BASED QUANTITATION 有权
标题翻译：用于基于质谱质谱法定量的背景校正的方法和系统
公开(公告)号：US20090065686A1
公开(公告)日：2009-03-12
申请号：US12208277
申请日：2008-09-10
申请人： Ignat V. Shilov , Wilfred H. Tang
发明人： Ignat V. Shilov , Wilfred H. Tang
IPC分类号： G01D18/00 , B01D59/44
CPC分类号： H01J49/004 , Y10T436/105831
摘要： A corrected reporter ion intensity is calculated in tandem mass spectrometry based quantitation using isobaric labels. An analyte in each of two or more samples of a mixture of samples is labeled with a different isobaric label. The analyte is eluted from the mixture of samples and intensities of the eluting analyte are measured. An analyte intensity is selected at each of at least two times from the measured intensities. Tandem mass spectrometry is performed on the eluting analyte at each of the at least two times. A plurality of reporter ion intensities is produced. A system of linear equations is created expressing each reporter ion intensity of the plurality of reporter ion intensities as a sum of a background noise intensity and the product of a fragmentation efficiency and an analyte intensity. A corrected reporter ion intensity is calculated from a solution of the system of linear equations.
摘要翻译：在使用等压标记的基于串联质谱法的定量中计算校正的报告离子强度。样品混合物的两个或更多个样品中的每一个中的分析物用不同的同量异序标记物标记。分析物从样品混合物中洗脱，并测量洗脱分析物的强度。从测量的强度中选择至少两次的分析物强度。在洗脱分析物中进行至少两次的串联质谱分析。产生多个报道离子强度。产生线性方程式的系统，表示多个报告离子强度的每个报告离子强度作为背景噪声强度和碎裂效率与分析物强度的乘积之和。从线性方程组的解求出校正的报道离子强度。

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