专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

1. 发明申请

US20050191689A1 Novel nucleic acid base pair 失效
标题翻译：新型核酸碱基对
公开(公告)号：US20050191689A1
公开(公告)日：2005-09-01
申请号：US11078607
申请日：2005-03-10
申请人： Ichiro Hirao , Masahide Ishikawa , Tsuyoshi Fujihara , Shigeyuki Yokoyama
发明人： Ichiro Hirao , Masahide Ishikawa , Tsuyoshi Fujihara , Shigeyuki Yokoyama
IPC分类号： C07H11/04 , C07H19/207 , C07H21/00 , C12N1/21 , C12N15/11 , C12P21/02 , C12Q1/68 , C07H21/04
CPC分类号： C07H11/04 , C07H19/207 , C07H21/00 , C12N15/11 , C12P21/02
摘要： A novel artificial nucleic acid base pair which is obtained by forming a selective base pair by introducing a group having steric hindrance (preferably a group having steric hindrance and static repulsion and a stacking effect) and can be recognized by a polymerase such as DNA polymerase; a novel artificial gene; and a method of designing nucleic acid bases so as to form a selective base pair with the use of steric hindrance, static repulsion and stacking effect at the base moiety of the nucleic acid. An artificial nucleic acid comprising these bases; a process for producing the same; a codon containing the same; a nucleic acid molecule containing the same; a process for producing a non-natural gene by using the same; a process for producing a novel protein by using the above nucleic acid molecule or non-natural gene, and the like.
摘要翻译：通过引入具有空间位阻的基团（优选具有空间位阻和静电排斥和堆叠效应的基团）形成选择性碱基对而获得的新型人造核酸碱基对，可以通过聚合酶如DNA聚合酶识别; 一种新型人工基因; 以及设计核酸碱基以在核酸的碱基部分上使用空间位阻，静电排斥和堆叠效应形成选择性碱基对的方法。包含这些碱的人造核酸; 其制造方法; 含有相同的密码子; 含有该核酸分子的核酸分子; 通过使用该方法生产非天然基因的方法; 通过使用上述核酸分子或非天然基因来生产新型蛋白质的方法等。

2. 发明授权

US07667031B2 Nucleic acid base pair 失效
标题翻译：核酸碱基对
公开(公告)号：US07667031B2
公开(公告)日：2010-02-23
申请号：US11078607
申请日：2005-03-10
申请人： Ichiro Hirao , Masahide Ishikawa , Tsuyoshi Fujihara , Shigeyuki Yokoyama
发明人： Ichiro Hirao , Masahide Ishikawa , Tsuyoshi Fujihara , Shigeyuki Yokoyama
IPC分类号： C07H21/00 , C07H21/04 , C07H19/06 , C07H19/048
CPC分类号： C07H11/04 , C07H19/207 , C07H21/00 , C12N15/11 , C12P21/02
摘要： A novel artificial nucleic acid base pair which is obtained by forming a selective base pair by introducing a group having steric hindrance (preferably a group having steric hindrance and static repulsion and a stacking effect) and can be recognized by a polymerase such as DNA polymerase; a novel artificial gene; and a method of designing nucleic acid bases so as to form a selective base pair with the use of steric hindrance, static repulsion and stacking effect at the base moiety of the nucleic acid. An artificial nucleic acid comprising these bases; a process for producing the same; a codon containing the same; a nucleic acid molecule containing the same; a process for producing a non-natural gene by using the same; a process for producing a novel protein by using the above nucleic acid molecule or non-natural gene, and the like.
摘要翻译：通过引入具有空间位阻的基团（优选具有空间位阻和静电排斥和堆叠效应的基团）形成选择性碱基对而获得的新型人造核酸碱基对，可以通过聚合酶如DNA聚合酶识别; 一种新型人工基因; 以及设计核酸碱基以在核酸的碱基部分上使用空间位阻，静电排斥和堆叠效应形成选择性碱基对的方法。包含这些碱的人造核酸; 其制造方法; 含有相同的密码子; 含有该核酸分子的核酸分子; 通过使用该方法生产非天然基因的方法; 通过使用上述核酸分子或非天然基因来生产新型蛋白质的方法等。

3. 发明申请

US20090286247A1 Novel nucleic acid base pair 审中-公开
标题翻译：新型核酸碱基对
公开(公告)号：US20090286247A1
公开(公告)日：2009-11-19
申请号：US12459794
申请日：2009-07-07
申请人： Ichiro Hirao , Masahide Ishikawa , Tsuyoshi Fujihara , Shigeyuki Yokoyama
发明人： Ichiro Hirao , Masahide Ishikawa , Tsuyoshi Fujihara , Shigeyuki Yokoyama
IPC分类号： C12Q1/68 , C07H21/00 , C12P19/34 , C07K1/00 , C12N1/00
CPC分类号： C07H11/04 , C07H19/207 , C07H21/00 , C12N15/11 , C12P21/02
摘要： A novel artificial nucleic acid base pair which is obtained by forming a selective base pair by introducing a group having steric hindrance (preferably a group having steric hindrance and static repulsion and a stacking effect) and can be recognized by a polymerase such as DNA polymerase; a novel artificial gene; and a method of designing nucleic acid bases so as to form a selective base pair with the use of steric hindrance, static repulsion and stacking effect at the base moiety of the nucleic acid. An artificial nucleic acid comprising these bases; a process for producing the same; a codon containing the same; a nucleic acid molecule containing the same; a process for producing a non-natural gene by using the same; a process for producing a novel protein by using the above nucleic acid molecule or non-natural gene, and the like.
摘要翻译：通过引入具有空间位阻的基团（优选具有空间位阻和静电排斥和堆叠效应的基团）形成选择性碱基对而获得的新型人造核酸碱基对，可以通过聚合酶如DNA聚合酶识别; 一种新型人工基因; 以及设计核酸碱基以在核酸的碱基部分上使用空间位阻，静电排斥和堆叠效应形成选择性碱基对的方法。包含这些碱的人造核酸; 其制造方法; 含有相同的密码子; 含有该核酸分子的核酸分子; 通过使用该方法生产非天然基因的方法; 通过使用上述核酸分子或非天然基因来生产新型蛋白质的方法等。

4. 发明授权

US07101992B1 Nucleic acid base pair 有权
标题翻译：核酸碱基对
公开(公告)号：US07101992B1
公开(公告)日：2006-09-05
申请号：US09787196
申请日：2000-07-14
申请人： Ichiro Hirao , Masahide Ishikawa , Tsuyoshi Fujihara , Shigeyuki Yokoyama
发明人： Ichiro Hirao , Masahide Ishikawa , Tsuyoshi Fujihara , Shigeyuki Yokoyama
IPC分类号： C07H21/00 , C07H19/16 , C07H19/048
CPC分类号： C07H11/04 , C07H19/207 , C07H21/00 , C12N15/11 , C12P21/02
摘要： A novel artificial nucleic acid base pair which is obtained by forming a selective base pair by introducing a group having steric hindrance (preferably a group having steric hindrance and static repulsion and a stacking effect) and can be recognized by a polymerase such as DNA polymerase; a novel artificial gene; and a method of designing nucleic acid bases so as to form a selective base pair with the use of steric hindrance, static repulsion and stacking effect at the base moity of the nucleic acid. An artificial nucleic acid comprising these bases; a process for producing the same; a codon containing the same; a nucleic acid molecule containing the same; a process for producing a non-natural gene by using the same; a process for producing a novel protein by using the above nucleic acid molecule or non-natural gene, and the like.
摘要翻译：通过引入具有空间位阻的基团（优选具有空间位阻和静电排斥和堆叠效应的基团）形成选择性碱基对而获得的新型人造核酸碱基对，可以通过聚合酶如DNA聚合酶识别; 一种新型人工基因; 以及设计核酸碱基的方法，以便在核酸的基础上使用空间位阻，静电排斥和堆叠效应形成选择性碱基对。包含这些碱的人造核酸; 其制造方法; 含有相同的密码子; 含有该核酸分子的核酸分子; 通过使用该方法生产非天然基因的方法; 通过使用上述核酸分子或非天然基因来生产新型蛋白质的方法等。

5. 发明授权

US07745417B2 Nucleosides or nucleotides having novel unnatural bases and use thereof 有权
标题翻译：具有新的非天然碱基的核苷或核苷及其用途
公开(公告)号：US07745417B2
公开(公告)日：2010-06-29
申请号：US10521454
申请日：2003-02-28
申请人： Ichiro Hirao , Shigeyuki Yokoyama , Michiko Hirao , Tsuneo Mitsui
发明人： Ichiro Hirao , Shigeyuki Yokoyama , Michiko Hirao , Tsuneo Mitsui
IPC分类号： A61K31/70 , C07H19/00 , C07H21/00 , C07H21/02
CPC分类号： C07H11/04 , C07H7/06
摘要： The object of the present invention is to provide a nucleoside or nucleotide having an unnatural base. The nucleoside or nucleotide of the present invention has a 5-substituted-2-oxo(1H)-pyridin-3-yl group as a base. Preferably, the 5-position of the above base is substituted with a substituent selected from the group consisting of the following: 1) a photoreactive group selected from iodine and bromine; 2) an alkenyl group, an alkynyl group or an amino group, or a derivative thereof; 3) biotin or a derivative thereof; and 4) a fluorescent molecule selected from fluorescein, 6-carboxyfluorescein, tetramethyl-6-carboxyrhodamine, and derivatives thereof.
摘要翻译：本发明的目的是提供具有非天然碱基的核苷或核苷酸。本发明的核苷或核苷酸具有5-取代-2-氧代（1H） - 吡啶-3-基作为碱基。优选地，上述碱的5位被选自下列的取代基取代：1）选自碘和溴的光反应性基团; 2）烯基，炔基或氨基或其衍生物; 3）生物素或其衍生物; 和4）选自荧光素，6-羧基荧光素，四甲基-6-羧基罗丹明及其衍生物的荧光分子。

6. 发明申请

US20100036111A1 METHOD FOR REPLICATING NUCLEIC ACIDS AND NOVEL UNNATURAL BASE PAIRS 有权
标题翻译：用于替代核酸和新型基础配对的方法
公开(公告)号：US20100036111A1
公开(公告)日：2010-02-11
申请号：US12096517
申请日：2006-12-07
申请人： Ichiro Hirao , Shigeyuki Yokoyama
发明人： Ichiro Hirao , Shigeyuki Yokoyama
IPC分类号： C12P19/34 , C07H19/04 , C07H19/20
CPC分类号： C12P19/34 , C07H19/04 , C07H21/00 , C12Q1/6844 , C12Q2525/101 , C12Q2525/117 , C12Q2525/113
摘要： The present invention relates to a method for nucleic acid replication and novel artificial base pairs.The method of the present invention for nucleic acid replication is characterized in that a deoxyribonucleoside 5′-triphosphate, in which the hydroxyl group of phosphoric acid at the γ-position is substituted with a group selected from the group consisting of an amino group, a methylamino group, a dimethylamino group, a mercapto group and a fluoro group, is used as a substrate during replication reaction. The novel artificial base pairs of the present invention are characterized in that 7-(2-thienyl)-imidazo[4,5-b]pyridine (Ds) or an analog thereof forms a base pair with pyrrole-2-carbaldehyde (Pa) or an analog thereof.
摘要翻译：本发明涉及核酸复制的方法和新的人造碱基对。用于核酸复制的本发明的方法的特征在于，其中在γ-位上的磷酸的羟基被选自下列的基团取代的脱氧核糖核苷5'-三磷酸：氨基，甲基氨基，二甲基氨基，巯基和氟基在复制反应期间用作底物。本发明的新型人造碱基对的特征在于7-（2-噻吩基） - 咪唑并[4,5-b]吡啶（Ds）或其类似物与吡咯-2-甲醛（Pa）形成碱基对，或其类似物。

7. 发明申请

US20090275017A1 Novel Nucleoside or Nucleotide Derivative and Use Thereof 失效
标题翻译：新型核苷或其衍生物及其用途
公开(公告)号：US20090275017A1
公开(公告)日：2009-11-05
申请号：US11667146
申请日：2005-11-08
申请人： Ichiro Hirao , Shigeyuki Yokoyama
发明人： Ichiro Hirao , Shigeyuki Yokoyama
IPC分类号： C12Q1/68 , C07H21/00 , C07H21/02 , C07H21/04
CPC分类号： C07H21/02
摘要： The object of the present invention is to provide a nucleoside or nucleotide having a 5-substituted-2-oxo(1H)pyridin-3-yl group as a base, as well as a method using the same.In one embodiment, the nucleoside or nucleotide of the present invention has a fluorescent dye selected from the group consisting of 5-FAM, 6-FAM, 5-TAMRA, 6-TAMRA, DANSYL, 5-HEX, 6-HEX, 5-TET, 6-TET, 5-ROX and 6-ROX or a quencher dye selected from the group consisting of DABCYL, BHQ1 and BHQ2, which is attached either directly or through a linker to the 5-position of the above base.
摘要翻译：本发明的目的是提供具有5-取代-2-氧代（1H）吡啶-3-基作为碱的核苷或核苷酸，以及使用其的方法。在一个实施方案中，本发明的核苷或核苷酸具有选自5-FAM，6-FAM，5-TAMRA，6-TAMRA，DANSYL，5-HEX，6-HEX，5- TET，6-TET，5-ROX和6-ROX或选自DABCYL，BHQ1和BHQ2的猝灭剂染料，其直接或通过接头连接到上述碱基的5位上。

8. 发明授权

US09285319B2 Nucleic acid base analogs with quenching and fluorescent activities and applications thereof 有权
标题翻译：具有淬灭和荧光活性的核酸碱基类似物及其应用
公开(公告)号：US09285319B2
公开(公告)日：2016-03-15
申请号：US13642111
申请日：2011-04-21
申请人： Ichiro Hirao , Michiko Hirao , Shigeyuki Yokoyama , Tsuneo Mitsui , Rie Yamashige
发明人： Ichiro Hirao , Michiko Hirao , Shigeyuki Yokoyama , Tsuneo Mitsui , Rie Yamashige
IPC分类号： C12Q1/68 , C07H21/02 , C07H21/04 , G01N21/64 , C07H19/044
CPC分类号： G01N21/6486 , C07H19/044 , C12Q1/6818 , C12Q2525/117
摘要： It is an object of the present invention to provide quenching or fluorescent nucleic acid base analogs and applications thereof. The quencher of the present invention has a 2-nitropyrrole structure represented by Formula I:[Formula 1] (in Formula I, R1 and R2 are groups independently selected from the group consisting of: ribose and deoxyribose; hydrogen, hydroxyl and SH groups, and halogens; substituted or unsubstituted alkyl, alkenyl, and alkynyl groups each having 2 to 10 carbon atoms; one or more five-membered heterocyclic rings, one or more six-membered heterocyclic rings, and one or more fused heterocyclic rings, these heterocylic rings containing nitrogen or sulfur, and one or more aromatic rings; sugars, sugar chains, amino acids, and peptides; and fluorescent molecules linked via linkers).
摘要翻译：本发明的目的是提供淬灭或荧光核酸碱基类似物及其应用。本发明的猝灭剂具有由式I表示的2-硝基吡咯结构：[式1]（式I中，R 1和R 2分别独立地选自：核糖和脱氧核糖;氢，羟基和SH基团，卤素;取代或未取代的各自具有2-10个碳原子的烷基，烯基和炔基;一个或多个五元杂环，一个或多个六元杂环和一个或多个稠合杂环，这些杂环含有氮或硫，以及一个或多个芳环;糖，糖链，氨基酸和肽;以及通过接头连接的荧光分子）。

9. 发明授权

US08715984B2 Modified tRNA containing unnatural base and use thereof 失效
标题翻译：含有非天然碱的修饰的tRNA及其用途
公开(公告)号：US08715984B2
公开(公告)日：2014-05-06
申请号：US12866720
申请日：2009-02-03
申请人： Shun-ichi Sekine , Ryuya Fukunaga , Shigeyuki Yokoyama , Ichiro Hirao , Yoko Harada
发明人： Shun-ichi Sekine , Ryuya Fukunaga , Shigeyuki Yokoyama , Ichiro Hirao , Yoko Harada
IPC分类号： C12N9/00 , C12P21/06
CPC分类号： C12N15/67 , C12N15/11 , C12N2310/33 , C12P21/02 , C12Q1/25 , C12Q1/48 , G01N2500/00
摘要： In the method for introducing a noncanonical amino acid residue into a desired position in a protein, the structure of tRNA is so modified as to have improved affinity for aminoacyl-tRNA synthetase or improved specificity to aminoacyl-tRNA synthetase. An unnatural base is contained at any position in tRNA, whereby the efficiency of aminoacylation of the tRNA with a noncanonical amino acid can be improved.
摘要翻译：在将非氨基酸氨基酸残基引入蛋白质中所需位置的方法中，对tRNA的结构进行了修饰，以改善对氨基-tRNA合成酶的亲和力或提高了对氨基-tRNA合成酶的特异性。在tRNA中的任何位置都含有非天然碱基，从而可以提高tRNA与非科学氨基酸的氨酰化效率。

10. 发明授权

US08686130B2 Artificial fluorescent bases 有权
标题翻译：人造荧光基
公开(公告)号：US08686130B2
公开(公告)日：2014-04-01
申请号：US13500303
申请日：2010-10-06
申请人： Ichiro Hirao , Michiko Hirao , Shigeyuki Yokoyama , Tsuneo Mitsui
发明人： Ichiro Hirao , Michiko Hirao , Shigeyuki Yokoyama , Tsuneo Mitsui
IPC分类号： C07H19/00 , C07H19/22
CPC分类号： C07D471/04 , C07D473/32 , C07H19/173 , C07H19/23 , C09K11/06 , C09K2211/1074
摘要： The present invention relates to novel unnatural fluorescent nucleic acid bases, that is, a purine base, a 1-deazapurine base, and a 1,7-deazapurine base each having a functional group which consists of two or more heterocyclic moieties linked together, at the 6-position thereof (the 6-position of purine ring). The present invention also relates to a compound containing the unnatural base, a derivative thereof, and a nucleic acid containing a nucleotide having the unnatural base. The present invention also relates to a method of preparing the nucleic acid. The unnatural base of the present invention has excellent fluorescence characteristics and also has excellent properties as a universal base.
摘要翻译：本发明涉及新型非天然荧光核酸碱基，即嘌呤碱，1-脱氮嘌呤碱和1,7-deazapurine碱，其各自具有由两个或多个连接在一起的杂环基部分组成的官能团，在其6-位（嘌呤环的6-位）。本发明还涉及含有非天然碱，其衍生物和含有具有非天然碱基的核苷酸的核酸的化合物。本发明还涉及制备核酸的方法。本发明的非天然碱具有优异的荧光特性，也具有优异的作为通用基材的特性。

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式