专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

1. 发明授权

US5824559A Method of analyzing 5-hydroxyindoles and catecholamines, and a device for performing the same 失效
标题翻译：分析5-羟基吲哚和儿茶酚胺的方法及其用途
公开(公告)号：US5824559A
公开(公告)日：1998-10-20
申请号：US664909
申请日：1996-06-17
申请人： Hitoshi Nohta , Masatoshi Yamaguchi , Junichi Ishida , Kivoshi Zaitsu , Hiroko Iida
发明人： Hitoshi Nohta , Masatoshi Yamaguchi , Junichi Ishida , Kivoshi Zaitsu , Hiroko Iida
IPC分类号： G01N33/52 , G01N33/48 , G01N21/76 , G01N30/02
CPC分类号： G01N33/52 , Y10T436/14 , Y10T436/145555 , Y10T436/17 , Y10T436/173845 , Y10T436/203332 , Y10T436/25
摘要： A method and a device for the analysis of 5-hydroxyindoles or catecholamines with high sensitivity. New chemiluminescence labeling agents, 6-aminomethylphthalhydrazide or 1,2-bis(phthalhydrazino)ethylenediamine, are reacted with 5-hydroxyindoles or catecholamines to form their stable derivatives. The derivatives emit strong luminescence in the presence of an oxidizing agent. In the chemiluminescence detection method, there is extremely low background noise and thus the method enables analysis with high sensitivity. The analysis of 5-hydroxyindoles or catecholamines in a sample is enabled by a method (1), in which each of the above-mentioned components is separated, reacted with said chemiluminescence labeling agent to form its derivative, and then chemiluminescent emitted by the reaction of the derivative with an oxidizing agent is detected, or by another method (2), in which the mixture of 5-hydroxyindoles or catecholamines in a sample is reacted with the chemiluminescence labeling agent to form its derivative mixture, subsequently each derivative is separated, and then chemiluminescent emitted by the reaction of the derivative with an oxidizing agent is detected.
摘要翻译：用于分析高灵敏度的5-羟基吲哚或儿茶酚胺的方法和装置。新化学发光标记试剂，6-氨基甲基邻苯二甲酰肼或1,2-双（邻苯二甲酰肼基）乙二胺与5-羟基吲哚或儿茶酚胺反应形成其稳定的衍生物。衍生物在氧化剂存在下发出强烈的发光。在化学发光检测方法中，具有极低的背景噪声，因此该方法能够以高灵敏度进行分析。通过方法（1）可以分析样品中的5-羟基吲哚或儿茶酚胺，其中将上述各成分分离，与所述化学发光标记试剂反应形成其衍生物，然后由反应发出化学发光或通过另一种方法（2），其中将样品中的5-羟基吲哚或儿茶酚胺的混合物与化学发光标记试剂反应以形成其衍生物混合物，随后分离每种衍生物，然后检测通过衍生物与氧化剂的反应发射的化学发光。

2. 发明授权

US06333199B1 Method of analyzing bisphenols and polyphenols 失效
标题翻译：分析双酚和多酚的方法
公开(公告)号：US06333199B1
公开(公告)日：2001-12-25
申请号：US09458653
申请日：1999-12-10
申请人： Hitoshi Nohta , Hideyuki Yoshida , Masatoshi Yamaguchi
发明人： Hitoshi Nohta , Hideyuki Yoshida , Masatoshi Yamaguchi
IPC分类号： G01N3300
CPC分类号： G01N31/22 , Y10T436/13 , Y10T436/203332 , Y10T436/212
摘要： The method of analyzing polyphenols and bisphenols in accordance with the present invention comprises a step of reacting a bisphenol and/or a polyphenol with a labeling reagent having a pyrene group, so as to generate a fluorescent derivative; a step of irradiating the fluorescent derivative with excitation light and detecting fluorescence emitted from the fluorescent derivative; and a step of calculating an amount of the bisphenol and/or polyphenol in a test sample according to an intensity of thus detected fluorescence and a relationship between a known concentration of a bisphenol and/or a polyphenol contained in a standard sample and fluorescence intensity.
摘要翻译：根据本发明的分析多酚和双酚的方法包括使双酚和/或多酚与具有芘基的标记试剂反应的步骤，以产生荧光衍生物; 用激发光照射荧光衍生物并检测从荧光衍生物发出的荧光的步骤; 以及根据这样检测的荧光的强度和标准样品中所含的双酚和/或多酚的已知浓度与荧光强度之间的关系来计算测试样品中的双酚和/或多酚的量的步骤。

3. 发明授权

US06242430B1 Cyclodextrin-based rotaxane dyes, labeling agent using the dye, and a method for labeling 失效
标题翻译：基于环糊精的轮烷染料，使用染料的标记试剂和标记方法
公开(公告)号：US06242430B1
公开(公告)日：2001-06-05
申请号：US09301635
申请日：1999-04-29
申请人： Tomomi Suzuki , Hitoshi Nohta , Shigetoshi Okazaki
发明人： Tomomi Suzuki , Hitoshi Nohta , Shigetoshi Okazaki
IPC分类号： A61K3120
CPC分类号： B82Y5/00 , A61K47/6951 , C09B57/00
摘要： This invention provides rotaxane type dyes. Specifically, the rotaxane type dye according to the invention has a rotaxane structure which binds dye molecules to both of the termini of a chain group penetrating a cyclodextrin ring, which possesses excellent water-solubility, and which is capable of having a plurality of different dyes.
摘要翻译：本发明提供了轮烷型染料。具体地说，根据本发明的轮烷型染料具有将染料分子结合到贯穿环糊精环的链基团的两末端的轮烷结构，其具有优异的水溶性，并且能够具有多种不同的染料。

4. 发明授权

US06207365B1 Fluorescent enzyme substrates and method for determining enzyme activities 失效
标题翻译：荧光酶底物和测定酶活性的方法
公开(公告)号：US06207365B1
公开(公告)日：2001-03-27
申请号：US09125288
申请日：1998-08-14
申请人： Hirofumi Shiono , Hitoshi Nohta , Chika Utsuyama
发明人： Hirofumi Shiono , Hitoshi Nohta , Chika Utsuyama
IPC分类号： C12Q100
CPC分类号： C12Q1/34 , C12Q2334/00 , C12Q2334/20
摘要： The enzyme substrate according to this invention has within its molecule both a group to be cleaved by an enzyme reaction and a group that forms a strongly fluorescent coumarin derivative through intramolecular lactonization when cleaved by the enzyme reaction. Furthermore, the method for determining an enzyme activity according to this invention comprises conducting the enzyme reaction by the use of the enzyme substrate of the invention and determining the enzyme activity by means of the measurement of fluorescence of the coumarin derivative formed.
摘要翻译：根据本发明的酶底物在其分子内具有通过酶反应切割的基团和当通过酶反应切割时通过分子内内酯形成强荧光香豆素衍生物的基团。此外，根据本发明的确定酶活性的方法包括通过使用本发明的酶底物进行酶反应，并通过测量形成的香豆素衍生物的荧光来测定酶活性。

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式