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1. 发明申请

US20080268497A1 TREATMENT SOLUTION, METHOD FOR PRETREATMENT, METHOD FOR ACQUIRING INFORMATION AND METHOD FOR DETECTION 有权
标题翻译：治疗方案，预处理方法，获取信息的方法和检测方法
公开(公告)号：US20080268497A1
公开(公告)日：2008-10-30
申请号：US12098822
申请日：2008-04-07
申请人： Hiroyuki Hashimoto , Manabu Komatsu , Satoru Hatakeyama
发明人： Hiroyuki Hashimoto , Manabu Komatsu , Satoru Hatakeyama
IPC分类号： G01N1/30 , C12Q1/00
CPC分类号： G01N33/6848 , B82Y15/00 , G01N33/54313
摘要： The present invention intends to provide a high sensitivity method for identifying the type of a protein contained in each cell with a diameter of ten to several tens μm and a sample treatment solution required for it. The above is achieved by three steps of (1) treating a slice of a diseased tissue with an aqueous solution containing gold particles and a digestive enzyme, and digesting restrictively a protein of interest, (2) measuring a 2-dimensional distribution of fragmented peptides by TOF-SIMS, and (3) visualizing a 2-dimensional distribution of the target protein in the slice of the diseased tissue using the results of the proteome analysis and by means of a numerical analysis.
摘要翻译：本发明旨在提供用于鉴定直径为10至数十微米的每个电池中所含的蛋白质的类型的高灵敏度方法和所需的样品处理溶液。上述通过以下三个步骤实现：（1）用含有金颗粒和消化酶的水溶液处理患病组织的切片，并限制性地消化目的蛋白质，（2）测量片段肽的2维分布通过TOF-SIMS，以及（3）使用蛋白质组分析的结果并通过数值分析来显现病变组织切片中靶蛋白的二维分布。

2. 发明授权

US08084214B2 Treatment solution, method for pretreatment, method for acquiring information and method for detection 有权
标题翻译：治疗方案，预处理方法，获取信息的方法和检测方法
公开(公告)号：US08084214B2
公开(公告)日：2011-12-27
申请号：US12098822
申请日：2008-04-07
申请人： Hiroyuki Hashimoto , Manabu Komatsu , Satoru Hatakeyama
发明人： Hiroyuki Hashimoto , Manabu Komatsu , Satoru Hatakeyama
IPC分类号： G01N33/53
CPC分类号： G01N33/6848 , B82Y15/00 , G01N33/54313
摘要： The present invention intends to provide a high sensitivity method for identifying the type of a protein contained in each cell with a diameter of ten to several tens μm and a sample treatment solution required for it. The above is achieved by three steps of (1) treating a slice of a diseased tissue with an aqueous solution containing gold particles and a digestive enzyme, and digesting restrictively a protein of interest, (2) measuring a 2-dimensional distribution of fragmented peptides by TOF-SIMS, and (3) visualizing a 2-dimensional distribution of the target protein in the slice of the diseased tissue using the results of the proteome analysis and by means of a numerical analysis.
摘要翻译：本发明旨在提供用于鉴定直径为10至几十μm的每个电池中所含的蛋白质的类型的高灵敏度方法以及所需的样品处理溶液。上述通过以下三个步骤实现：（1）用含有金颗粒和消化酶的水溶液处理患病组织的切片，并限制性地消化目的蛋白质，（2）测量片段肽的2维分布通过TOF-SIMS，以及（3）使用蛋白质组分析的结果并通过数值分析来显现病变组织切片中靶蛋白的二维分布。

3. 发明申请

US20120289411A1 METHOD FOR SCREENING NUCLEIC ACID LIGAND 审中-公开
标题翻译：筛选核酸配体的方法
公开(公告)号：US20120289411A1
公开(公告)日：2012-11-15
申请号：US13511641
申请日：2011-02-23
申请人： Satoru Hatakeyama , Takahisa Ibii
发明人： Satoru Hatakeyama , Takahisa Ibii
IPC分类号： C40B30/04 , C40B20/00
CPC分类号： C12N15/1048 , G01N33/5308
摘要： Provided is a method for screening a library of candidate for a nucleic acid ligand. The method includes the steps of: (a) preparing a library of candidate of nucleic acid ligands; (b) contacting, under the absence of a target substance, the library with a supporting member binding to at least one of conserved sequence domains included in the ligand, and then separating and removing a ligand which does not form an intermolecular duplex; and (c) dissociating the intermolecular duplex by contacting, under the presence of the target substance, the target substance with the remaining ligand forming the intermolecular duplex obtained in step (b), and then separating and collecting a ligand having a specific secondary structure formed by the binding to the target substance, wherein the method includes at least one time of step (b) and at least one time of step (c).
摘要翻译：提供了筛选核酸配体候选文库的方法。该方法包括以下步骤：（a）制备核酸配体候选文库; （b）在不存在目标物质的情况下，使文库与支配构件接触，所述支持构件与包含在配体中的保守序列结构域中的至少一个结合，然后分离和除去不形成分子间双键的配体; 和（c）通过在靶物质的存在下使靶物质与形成步骤（b）中获得的分子间双环体的剩余配体接触，然后分离和收集形成特定二级结构的配体，使分子内双链体解离通过与目标物质的结合，其中所述方法包括步骤（b）的至少一个时间和步骤（c）的至少一个时间）。

4. 发明授权

US07541195B2 Substrate for biomolecule microarray, biomolecule microarray, device and method of promoting interaction, and method of detecting interaction 失效
标题翻译：用于生物分子微阵列的基质，生物分子微阵列，促进相互作用的装置和方法以及检测相互作用的方法
公开(公告)号：US07541195B2
公开(公告)日：2009-06-02
申请号：US10560584
申请日：2004-06-09
申请人： Hideo Tashiro , Yasumitsu Kondoh , Tokuji Kitsunai , Satoru Hatakeyama
发明人： Hideo Tashiro , Yasumitsu Kondoh , Tokuji Kitsunai , Satoru Hatakeyama
IPC分类号： G01N33/543
CPC分类号： G01N21/6452 , B01J19/0046 , B01J2219/00527 , B01J2219/00605 , B01J2219/0061 , B01J2219/00612 , B01J2219/00621 , B01J2219/00626 , B01J2219/0063 , B01J2219/00637 , B01J2219/00653 , B01J2219/0072 , B01J2219/00722 , B01J2219/00725 , B01L3/5085 , C40B40/06 , C40B40/10 , G01N21/253 , G01N21/6428 , G01N33/5438 , G01N2021/6482
摘要： The substrate for biomolecule microarray has one or more spots for immobilizing a biomolecule. The spot for immobilizing a biomolecule protrudes from the surface of the substrate and has a flat surface for spotting on the top thereof, at least the surface of the substrate around the protruding spot part, the lateral surface of the protruding spot part and the flat surface for spotting are comprised of an electrically conductive substance. Alternatively, the spot for immobilizing a biomolecule protrudes from the surface of the substrate and has a flat surface for spotting on the top thereof, the protruding spot parts adjacent each other border through the lateral surface of the protruding spot part, and at least the lateral surface of the protruding spot part and the flat surface for spotting are comprised of an electrically conductive substance. The biomolecule microarray comprises the above substrate and a biomolecule and the biomolecule is immobilized on at least the flat surface for spotting on the substrate. The device of promoting interaction between biomolecules comprises a biomolecule microarray having one or more biomolecule-immobilized spots on a substrate, an electrode provided so as to face the surface having the biomolecule-immobilized spots of the microarray, and a power source for applying an electric field between the microarray and the electrode. The method of promoting interaction between biomolecules employing the above device. The method of detecting interaction between biomolecules. Provided are a substrate having biomolecule immobilization regions of prescribed shape on a biomolecule microarray, and means by which the interaction of biomolecules is rapidly conducted, the interaction of trace quantities of sample is promoted, and the interaction is detected and analyzed rapidly and with high sensitivity.
摘要翻译：用于生物分子微阵列的底物具有用于固定生物分子的一个或多个斑点。用于固定生物分子的点从基板的表面突出，并且具有用于在其顶部上点样的平坦表面，至少在突出的点部分周围的基板的表面，突出的点部分的侧表面和平坦表面用于点样由导电物质组成。或者，用于固定生物分子的斑点从基板的表面突出，并且具有用于在其顶部上斑点的平坦表面，突出的点部分彼此相邻，通过突出的斑点部分的侧表面，并且至少横向突出点部分的表面和用于点样的平坦表面由导电物质组成。生物分子微阵列包括上述底物和生物分子，生物分子至少固定在平坦表面上以便在基底上进行点样。促进生物分子之间的相互作用的装置包括在基底上具有一个或多个生物分子固定斑点的生物分子微阵列，设置成面对具有微阵列的生物分子固定点的表面的电极，以及用于施加电微阵列和电极之间的场。促进使用上述装置的生物分子之间相互作用的方法。检测生物分子之间相互作用的方法。提供了一种在生物分子微阵列上具有规定形状的生物分子固定区的底物，以及快速进行生物分子相互作用的手段，促进了微量样品的相互作用，并快速，高灵敏度地检测和分析了相互作用。

5. 发明授权

US08183058B2 Target substance detection element, target substance detection method, and method for producing target substance detection element 有权
标题翻译：靶物质检测元件，目标物质检测方法以及靶物质检测元件的制造方法
公开(公告)号：US08183058B2
公开(公告)日：2012-05-22
申请号：US12524348
申请日：2008-03-17
申请人： Satoru Hatakeyama , Takashi Ikeda
发明人： Satoru Hatakeyama , Takashi Ikeda
IPC分类号： G01N33/543
CPC分类号： G01N33/54386 , G01N33/54393 , Y10S435/97 , Y10S436/807 , Y10T156/10
摘要： It is intended to provide a target substance detection element wherein a target substance capturing body for capturing target substances is immobilized with good orientation in a desired region on the surface of the target substance detection element, a method for producing the target substance detection element, and a detection method using the target substance detection element. The present invention provides a target substance detection element for detecting the presence or absence or concentration of a target substance in a sample, characterized in that: the target substance detection element includes at least a detection substrate including plural layers and a target substance capturing body immobilized on the surface of the detection substrate; the target substance capturing body has at least a first peptide region specifically recognizing a first layer of the plural layers constituting the detection substrate and binding to the first layer and a second peptide region specifically recognizing a second layer different from the first layer of the plural layers and binding to the second layer; and the first layer and the second layer are adjacent to each other.
摘要翻译：旨在提供一种目标物质检测元件，其中用于捕获目标物质的目标物质捕获体被固定在目标物质检测元件的表面上的期望区域中具有良好取向，该目标物质检测元件的制造方法和使用目标物质检测元件的检测方法。本发明提供了一种用于检测样品中目标物质的存在或不存在或浓度的目标物质检测元件，其特征在于：所述目标物质检测元件至少包括包含多层的检测基板和固定的靶物质捕获体在检测基板的表面上; 目标物质捕捉体具有至少第一肽区域，其具体地识别构成检测基板的多层的第一层并与第一层结合，以及特异性识别与多层的第一层不同的第二层的第二肽区域并与第二层结合; 并且第一层和第二层彼此相邻。

6. 发明申请

US20100047928A1 TARGET SUBSTANCE DETECTION ELEMENT, TARGET SUBSTANCE DETECTION METHOD, AND METHOD FOR PRODUCING TARGET SUBSTANCE DETECTION ELEMENT 有权
标题翻译：目标物质检测元件，目标物质检测方法及产生目标物质检测元件的方法
公开(公告)号：US20100047928A1
公开(公告)日：2010-02-25
申请号：US12524348
申请日：2008-03-17
申请人： Satoru Hatakeyama , Takashi Ikeda
发明人： Satoru Hatakeyama , Takashi Ikeda
IPC分类号： G01N33/543 , B29C65/00
CPC分类号： G01N33/54386 , G01N33/54393 , Y10S435/97 , Y10S436/807 , Y10T156/10
摘要： It is intended to provide a target substance detection element wherein a target substance capturing body for capturing target substances is immobilized with good orientation in a desired region on the surface of the target substance detection element, a method for producing the target substance detection element, and a detection method using the target substance detection element. The present invention provides a target substance detection element for detecting the presence or absence or concentration of a target substance in a sample, characterized in that: the target substance detection element includes at least a detection substrate including plural layers and a target substance capturing body immobilized on the surface of the detection substrate; the target substance capturing body has at least a first peptide region specifically recognizing a first layer of the plural layers constituting the detection substrate and binding to the first layer and a second peptide region specifically recognizing a second layer different from the first layer of the plural layers and binding to the second layer; and the first layer and the second layer are adjacent to each other.
摘要翻译：旨在提供一种目标物质检测元件，其中用于捕获目标物质的目标物质捕获体被固定在目标物质检测元件的表面上的期望区域中具有良好取向，该目标物质检测元件的制造方法和使用目标物质检测元件的检测方法。本发明提供了一种用于检测样品中目标物质的存在或不存在或浓度的目标物质检测元件，其特征在于：所述目标物质检测元件至少包括包含多层的检测基板和固定的靶物质捕获体在检测基板的表面上; 目标物质捕捉体具有至少第一肽区域，其具体地识别构成检测基板的多层的第一层并与第一层结合，以及特异性识别与多层的第一层不同的第二层的第二肽区域并与第二层结合; 并且第一层和第二层彼此相邻。

7. 发明申请

US20070004019A1 Device and method for introducing particle into cell and device and method for collecting particle from cell 失效
标题翻译：将颗粒引入细胞和装置的装置和方法以及从细胞中收集颗粒的方法
公开(公告)号：US20070004019A1
公开(公告)日：2007-01-04
申请号：US11165524
申请日：2005-06-24
申请人： Satoru Hatakeyama , Hideaki Okamoto , Norihiko Utsunomiya , Junta Yamamichi
发明人： Satoru Hatakeyama , Hideaki Okamoto , Norihiko Utsunomiya , Junta Yamamichi
IPC分类号： C12N13/00
CPC分类号： C12N13/00 , C12M35/02
摘要： A device for introducing fine magnetic particles into a cell by applying an electric field to a reaction field where a sample solution containing cells and the fine magnetic particles are introduced, in order to form pores in the surface of the cell, and then applying a magnetic field to the reaction field to introduce the fine magnetic particles into the cell through the pore(s).
摘要翻译：通过向导入含有电池和微细粒子的样品溶液的反应场施加电场，将细磁粒子引入电池的装置，以在电池表面形成孔，然后施加磁通过反应场将微细磁性颗粒引入细胞中。

8. 发明授权

US07989219B2 Bispecific capturing molecule 有权
标题翻译：双特异性捕获分子
公开(公告)号：US07989219B2
公开(公告)日：2011-08-02
申请号：US11913045
申请日：2006-05-30
申请人： Hidenori Shiotsuka , Satoru Hatakeyama , Tsuyoshi Nomoto , Masaru Kaieda , Junta Yamamichi
发明人： Hidenori Shiotsuka , Satoru Hatakeyama , Tsuyoshi Nomoto , Masaru Kaieda , Junta Yamamichi
IPC分类号： G01N33/53
CPC分类号： C07K16/40 , C07K2317/22 , C07K2317/31 , C07K2319/73 , G01N33/531 , G01N33/566 , G01N33/6854 , Y10S435/97 , Y10S436/81
摘要： A capturing molecule having an association containing a plurality of polypeptide chains that specifically bind to different sites of a target substance, characterized in that each of the polypeptide chains has a domain having a hypervariable loop structure at a binding site binding to the target substance and an association portion for forming the association, and the polypeptide chains are associated via the association portions present in the polypeptide chains.
摘要翻译：具有包含特异性结合靶物质的不同位点的多个多肽链的缔合的捕获分子，其特征在于，每个多肽链具有在结合位点与目标物质具有高变环结构的结构域，以及用于形成缔合的缔合部分和多肽链通过存在于多肽链中的缔合部分相关联。

9. 发明申请

US20090170220A1 BISPECIFIC CAPTURING MOLECULE 有权
标题翻译：双向捕获分子
公开(公告)号：US20090170220A1
公开(公告)日：2009-07-02
申请号：US11913045
申请日：2006-05-30
申请人： Hidenori Shiotsuka , Satoru Hatakeyama , Tsuyoshi Nomoto , Masaru Kaieda , Junta Yamamichi
发明人： Hidenori Shiotsuka , Satoru Hatakeyama , Tsuyoshi Nomoto , Masaru Kaieda , Junta Yamamichi
IPC分类号： G01N33/543 , C07K16/18 , B01J19/00
CPC分类号： C07K16/40 , C07K2317/22 , C07K2317/31 , C07K2319/73 , G01N33/531 , G01N33/566 , G01N33/6854 , Y10S435/97 , Y10S436/81
摘要： A capturing molecule having an association containing a plurality of polypeptide chains that specifically bind to different sites of a target substance, characterized in that each of the polypeptide chains has a domain having a hypervariable loop structure at a binding site binding to the target substance and an association portion for forming the association, and the polypeptide chains are associated via the association portions present in the polypeptide chains.
摘要翻译：具有包含特异性结合靶物质的不同位点的多个多肽链的缔合的捕获分子，其特征在于，每个多肽链具有在结合位点与目标物质具有高变环结构的结构域，以及用于形成缔合的缔合部分和多肽链通过存在于多肽链中的缔合部分相关联。

10. 发明申请

US20070037155A1 Substrate for biomolecule microarray, biomolecule microarray, device and method of promoting interaction, and method of detecting interaction 失效
标题翻译：用于生物分子微阵列的基质，生物分子微阵列，促进相互作用的装置和方法以及检测相互作用的方法
公开(公告)号：US20070037155A1
公开(公告)日：2007-02-15
申请号：US10560584
申请日：2004-06-09
申请人： Hideo Tashiro , Yasumitsu Kondoh , Tokuji Kitsunai , Satoru Hatakeyama
发明人： Hideo Tashiro , Yasumitsu Kondoh , Tokuji Kitsunai , Satoru Hatakeyama
IPC分类号： C12Q1/68 , G01N33/53 , C12M3/00
CPC分类号： G01N21/6452 , B01J19/0046 , B01J2219/00527 , B01J2219/00605 , B01J2219/0061 , B01J2219/00612 , B01J2219/00621 , B01J2219/00626 , B01J2219/0063 , B01J2219/00637 , B01J2219/00653 , B01J2219/0072 , B01J2219/00722 , B01J2219/00725 , B01L3/5085 , C40B40/06 , C40B40/10 , G01N21/253 , G01N21/6428 , G01N33/5438 , G01N2021/6482
摘要： The substrate for biomolecule microarray has one or more spots for immobilizing a biomolecule. The spot for immobilizing a biomolecule protrudes from the surface of the substrate and has a flat surface for spotting on the top thereof, at least the surface of the substrate around the protruding spot part, the lateral surface of the protruding spot part and the flat surface for spotting are comprised of an electrically conductive substance. Alternatively, the spot for immobilizing a biomolecule protrudes from the surface of the substrate and has a flat surface for spotting on the top thereof, the protruding spot parts adjacent each other border through the lateral surface of the protruding spot part, and at least the lateral surface of the protruding spot part and the flat surface for spotting are comprised of an electrically conductive substance. The biomolecule microarray comprises the above substrate and a biomolecule and the biomolecule is immobilized on at least the flat surface for spotting on the substrate. The device of promoting interaction between biomolecules comprises a biomolecule microarray having one or more biomolecule-immobilized spots on a substrate, an electrode provided so as to face the surface having the biomolecule-immobilized spots of the microarray, and a power source for applying an electric field between the microarray and the electrode. The method of promoting interaction between biomolecules employing the above device. The method of detecting interaction between biomolecules. Provided are a substrate having biomolecule immobilization regions of prescribed shape on a biomolecule microarray, and means by which the interaction of biomolecules is rapidly conducted, the interaction of trace quantities of sample is promoted, and the interaction is detected and analyzed rapidly and with high sensitivity.
摘要翻译：用于生物分子微阵列的底物具有用于固定生物分子的一个或多个斑点。用于固定生物分子的点从基板的表面突出，并且具有用于在其顶部上点样的平坦表面，至少在突出的点部分周围的基板的表面，突出的点部分的侧表面和平坦表面用于点样由导电物质组成。或者，用于固定生物分子的斑点从基板的表面突出，并且具有用于在其顶部上斑点的平坦表面，突出的点部分彼此相邻，通过突出的斑点部分的侧表面，并且至少横向突出点部分的表面和用于点样的平坦表面由导电物质组成。生物分子微阵列包括上述底物和生物分子，生物分子至少固定在平坦表面上以便在基底上进行点样。促进生物分子之间的相互作用的装置包括在基底上具有一个或多个生物分子固定斑点的生物分子微阵列，设置成面对具有微阵列的生物分子固定点的表面的电极，以及用于施加电微阵列和电极之间的场。促进使用上述装置的生物分子之间相互作用的方法。检测生物分子之间相互作用的方法。提供了一种在生物分子微阵列上具有规定形状的生物分子固定区的底物，以及快速进行生物分子相互作用的手段，促进了微量样品的相互作用，并快速，高灵敏度地检测和分析了相互作用。

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