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1. 发明申请

US20120225440A1 HEPATOCELLULAR CARCINOMA PROTEIN MARKER, AND METHOD FOR DETECTION OF HEPATOCELLULAR CARCINOMA USING THE SAME 审中-公开
标题翻译：肝细胞癌蛋白标记物及使用其检测肝细胞癌的方法
公开(公告)号：US20120225440A1
公开(公告)日：2012-09-06
申请号：US13471629
申请日：2012-05-15
申请人： Hirotaka Minagawa , Kenji Miyazaki , Yo Tabuse , Kenichi Kamijo , Shuichi Kaneko
发明人： Hirotaka Minagawa , Kenji Miyazaki , Yo Tabuse , Kenichi Kamijo , Shuichi Kaneko
IPC分类号： G01N33/574 , G01N27/62 , G01N21/64
CPC分类号： C07K14/4748 , G01N33/57438
摘要： A method of detecting hepatocellular carcinoma includes using an isolated protein including an amino acid sequence represented by SEQ ID NO: 1.
摘要翻译：检测肝细胞癌的方法包括使用包含SEQ ID NO：1所示氨基酸序列的分离蛋白。

2. 发明申请

US20100248256A1 HEPATOCELLULAR CARCINOMA PROTEIN MARKER, AND METHOD FOR DETECTION OF HEPATOCELLULAR CARCINOMA USING THE SAME 失效
标题翻译：肝细胞癌蛋白标记物及使用其检测肝细胞癌的方法
公开(公告)号：US20100248256A1
公开(公告)日：2010-09-30
申请号：US12452216
申请日：2008-06-16
申请人： Hirotaka Minagawa , Kenji Miyazaki , Yo Tabuse , Kenichi Kamijo , Shuichi Kaneko
发明人： Hirotaka Minagawa , Kenji Miyazaki , Yo Tabuse , Kenichi Kamijo , Shuichi Kaneko
IPC分类号： G01N33/53 , C07K14/435
CPC分类号： C07K14/4748 , G01N33/57438
摘要： Provided are: a method of assessing hepatocellular carcinoma by using a protein with a different phosphorylated state in hepatocellular carcinoma cells compared with non-hepatocellular carcinoma cells; and a hepatocellular carcinoma protein marker for detecting hepatocellular carcinoma formed of the protein. The hepatocellular carcinoma protein marker for detecting hepatocellular carcinoma includes tumor rejection antigen gp96 formed of the amino acid represented by SEQ ID NO: 1, and is measured for its phosphorylated state to detect the presence or absence of hepatocellular carcinoma.
摘要翻译：提供：与非肝细胞癌细胞相比，通过使用肝细胞癌细胞中具有不同磷酸化状态的蛋白质来评估肝细胞癌的方法; 和用于检测由蛋白质形成的肝细胞癌的肝细胞癌蛋白标记物。用于检测肝细胞癌的肝细胞癌蛋白标志物包括由SEQ ID NO：1表示的氨基酸形成的肿瘤排斥抗原gp96，并测量其磷酸化状态以检测肝细胞癌的存在或不存在。

3. 发明授权

US08207301B2 Hepatocellular carcinoma protein marker 失效
标题翻译：肝细胞癌蛋白标记
公开(公告)号：US08207301B2
公开(公告)日：2012-06-26
申请号：US12452216
申请日：2008-06-16
申请人： Hirotaka Minagawa , Kenji Miyazaki , Yo Tabuse , Kenichi Kamijo , Shuichi Kaneko
发明人： Hirotaka Minagawa , Kenji Miyazaki , Yo Tabuse , Kenichi Kamijo , Shuichi Kaneko
IPC分类号： C07K14/435
CPC分类号： C07K14/4748 , G01N33/57438
摘要： Provided are: a method of assessing hepatocellular carcinoma by using a protein with a different phosphorylated state in hepatocellular carcinoma cells compared with non-hepatocellular carcinoma cells; and a hepatocellular carcinoma protein marker for detecting hepatocellular carcinoma formed of the protein. The hepatocellular carcinoma protein marker for detecting hepatocellular carcinoma includes tumor rejection antigen gp96 formed of the amino acid represented by SEQ ID NO: 1, and is measured for its phosphorylated state to detect the presence or absence of hepatocellular carcinoma.
摘要翻译：提供：与非肝细胞癌细胞相比，通过使用肝细胞癌细胞中具有不同磷酸化状态的蛋白质来评估肝细胞癌的方法; 和用于检测由蛋白质形成的肝细胞癌的肝细胞癌蛋白标记物。用于检测肝细胞癌的肝细胞癌蛋白标志物包括由SEQ ID NO：1表示的氨基酸形成的肿瘤排斥抗原gp96，并测量其磷酸化状态以检测肝细胞癌的存在或不存在。

4. 发明申请

US20070026456A1 Method of biomolecule analysis and method of identifying biomolecule therewith 审中-公开
标题翻译：生物分子分析方法及其识别生物分子的方法
公开(公告)号：US20070026456A1
公开(公告)日：2007-02-01
申请号：US10570205
申请日：2004-09-02
申请人： Kenichi Kamijo , Hisao Kawaura , Toru Sano , Yo Tabuse , Hirotaka Minagawa , Kenji Miyazaki
发明人： Kenichi Kamijo , Hisao Kawaura , Toru Sano , Yo Tabuse , Hirotaka Minagawa , Kenji Miyazaki
IPC分类号： G01N33/53
CPC分类号： G01N33/6803
摘要： A biomolecule is analyzed with high accuracy. A biomolecule is analyzed with high likelihood. A biomolecule as an analysis subject is modified with a marker binding or adsorbing to only its specific portion (S101). Next, the biomolecule modified with the marker is developed on a base plate (S102). The arrangement of the biomolecule on the base plate is detected using a marker (S103). Then, scanning is performed along the shape of the biomolecule present on the detected position (S104). The biomolecule is analyzed based on an information relating to the shape or arrangement of the biomolecule obtained by scanning or an information relating to the arrangement of the marker on the biomolecule (S105).
摘要翻译：以高精度分析生物分子。分析生物分子的可能性很高。作为分析对象的生物分子用仅结合或吸附到其特定部分的标记进行修饰（S 101）。接下来，在基板上显影用标记物修饰的生物分子（S102）。使用标记检测基板上的生物分子的排列（S103）。然后，沿检测位置上存在的生物分子的形状进行扫描（S104）。基于与通过扫描获得的生物分子的形状或排列相关的信息或与生物分子上的标记的排列有关的信息来分析生物分子（S105）。

5. 发明申请

US20090319450A1 PROTEIN SEARCH METHOD AND DEVICE 审中-公开
标题翻译：蛋白质搜索方法和装置
公开(公告)号：US20090319450A1
公开(公告)日：2009-12-24
申请号：US12373675
申请日：2007-07-09
申请人： Reiji Teramoto , Hirotaka Minagawa , Kenichi Kamijo
发明人： Reiji Teramoto , Hirotaka Minagawa , Kenichi Kamijo
IPC分类号： G06F15/18 , G06F19/00 , G06N7/02
CPC分类号： G16B40/00 , G01N27/44773 , G16B20/00
摘要： A protein search method for searching for, as a target protein, a protein having direct or indirect relevance to information based on protein representation profiling data acquired by means of proteome analysis includes: determining, as a target protein, a protein that is relevant to the information based on significance of proteins obtained by using supervised learning from the information and the protein representation in the profiling data; and evaluating the performance of the target protein by means of evaluation data.
摘要翻译：一种蛋白质搜索方法，用于搜索基于通过蛋白质组分析获得的蛋白质表达谱分析数据与信息直接或间接相关的蛋白质的蛋白质搜索方法，包括：将作为靶蛋白的蛋白质确定为与基于通过使用来自信息的监督学习获得的蛋白质的信息和在分析数据中的蛋白质表示的信息; 并通过评估数据评估靶蛋白的表现。

6. 发明授权

US07651859B2 Method of analyzing c-terminal amino acid sequence of peptide 失效
标题翻译：分析肽末端氨基酸序列的方法
公开(公告)号：US07651859B2
公开(公告)日：2010-01-26
申请号：US10538305
申请日：2003-12-04
申请人： Kenji Miyazaki , Akira Tsugita , Kenichi Kamijo , Takuji Nabetani
发明人： Kenji Miyazaki , Akira Tsugita , Kenichi Kamijo , Takuji Nabetani
IPC分类号： G01N33/00
CPC分类号： G01N33/6821 , G01N33/6842
摘要： The present invention provides, as for a method for analyzing the C-terminal amino acid sequence of a peptide by using a reaction for successively releasing the C-terminal amino acids of the peptide, which method can suppress, when successively releasing the C-terminal amino acids of a peptide of long amino acid length, such a undesirable side reaction as cleavage of peptide bond in the intermediate position of the peptide and can carry out the chemical treatment thereof under widely applicable conditions, a following method wherein a dry sample of a peptide with long amino acid length is beforehand subjected to an N-acylation treatment; by using a reaction reagent where an alkanoic acid anhydride is combined with a small amount of a perfluoroalkanoic acid, successive release of C-terminal amino acids is conducted under mild conditions; a hydrolysis treatment is applied; then, selective fragmentization at site of arginine residue is performed by digestion by trypsin; thereafter, decreases in molecular weight are measured for the C-terminal side fragments derived from a series of reaction products by analysis in negative mode of a MALDI-TOF-MS apparatus; thereby, the C-terminal amino acid sequence of the peptide sample is identified.
摘要翻译：本发明提供了通过使用连续释放肽的C末端氨基酸的反应来分析肽的C末端氨基酸序列的方法，该方法可以在连续释放C端具有长氨基酸长度的肽的氨基酸，这种不利的副反应，如在肽的中间位置处的肽键断裂，并且可以在广泛适用的条件下进行化学处理，以下方法，其中将具有长氨基酸长度的肽预先进行N-酰化处理; 通过使用其中链烷酸酐与少量全氟链烷酸组合的反应试剂，在温和条件下连续释放C-末端氨基酸; 应用水解处理; 然后通过胰蛋白酶消化进行精氨酸残基位点的选择性碎裂; 此后，通过MALDI-TOF-MS装置的负模式的分析，测量衍生自一系列反应产物的C末端侧片段的分子量的降低; 鉴定出肽样品的C-末端氨基酸序列。

7. 发明申请

US20080213911A1 Method of Analyzing C-Terminal Amino Acid Sequence of Peptide 失效
标题翻译：分析C端氨基酸序列的方法
公开(公告)号：US20080213911A1
公开(公告)日：2008-09-04
申请号：US10589495
申请日：2004-08-24
申请人： Kenji Miyazaki , Akira Tsugita , Kenichi Kamijo
发明人： Kenji Miyazaki , Akira Tsugita , Kenichi Kamijo
IPC分类号： G01N33/00
CPC分类号： G01N33/6842 , G01N33/6821 , Y10T436/24
摘要： An analyte peptide is selectively degraded sequentially by using an alkanoic anhydride (S101). The original peptide and a series of degradation reaction products having peptide in which one or more C-terminal-sided amino acids are deleted, are subjected to a certain posttreatment (S102). The molecular weight of the reaction products is measured by mass spectrometry (S103). And, the amino acid sequence of the original peptide from C-terminal is determined, based on the molecular weight obtained by mass spectrometry (S104).
摘要翻译：通过使用链烷酸酐顺序地降解分析物肽（S101）。将具有缺失一个或多个C末端氨基酸的肽的原始肽和一系列降解反应产物进行一定的后处理（S102）。通过质谱法测定反应产物的分子量（S103）。并且，基于通过质谱法获得的分子量测定来自C末端的原始肽的氨基酸序列（S104）。

8. 发明申请

US20060177887A1 Method of analyzing a protein 审中-公开
标题翻译：分析蛋白质的方法
公开(公告)号：US20060177887A1
公开(公告)日：2006-08-10
申请号：US11322320
申请日：2006-01-03
申请人： Kenji Miyazaki , Hiroaki Torii , Kenichi Kamijo , Akira Tsugita
发明人： Kenji Miyazaki , Hiroaki Torii , Kenichi Kamijo , Akira Tsugita
IPC分类号： C12Q1/37 , G06F19/00 , G01N33/00
CPC分类号： G01N33/6842 , G01N30/6095 , G01N30/7233 , G01N33/6821 , G01N33/6848
摘要： Provided a method of acquiring information on internal sequence which is applicable to a trace amount of protein and has a high reliability. A protein to be analyzed is limitedly cleaved at a specified position of amino acid to prepare a mixture of a plurality of peptide fragments; one or more peptide fragments are isolated and purified from the peptide fragment mixture; C-terminal amino acids of the isolated and purified peptide fragment are successively released by chemical reaction to prepare a mixture containing a series of resulting products; mass spectrometry is applied to both the reaction products of the successive truncation and unreacted peptide fragment not subjected to the successive truncation reaction; then results of the mass spectrometry is analyzed to acquire chemical structure information of the target protein.
摘要翻译：提供一种获取适用于微量蛋白质并具有高可靠性的内部序列信息的方法。要分析的蛋白质在氨基酸的指定位置被有限地切割以制备多个肽片段的混合物; 从肽片段混合物中分离和纯化一个或多个肽片段; 分离和纯化的肽片段的C-末端氨基酸通过化学反应连续释放，制备含有一系列产物的混合物; 对连续截断的反应产物和未进行连续截短反应的未反应的肽片段进行质谱分析; 然后分析质谱结果，获得目标蛋白质的化学结构信息。

9. 发明授权

US07384790B2 Method of analyzing peptide for determining C-terminal amino acid sequence 失效
标题翻译：分析肽用于测定C末端氨基酸序列的方法
公开(公告)号：US07384790B2
公开(公告)日：2008-06-10
申请号：US10489198
申请日：2003-03-24
申请人： Kenji Miyazaki , Akira Tsugita , Naoyuki Takahashi , Takuji Nabetani , Toshimasa Yamazaki , Kenichi Kamijo
发明人： Kenji Miyazaki , Akira Tsugita , Naoyuki Takahashi , Takuji Nabetani , Toshimasa Yamazaki , Kenichi Kamijo
IPC分类号： G01N33/68 , G01N33/483 , G01N27/12
CPC分类号： G01N33/6821 , G01N33/6842
摘要： The present invention provides a method for analyzing the C-terminal amino acid sequence of a peptide by applying reaction technique for successively releasing the C-terminal amino acids therefrom, which method can suppress, when releasing the C-terminal amino acids of the peptide in sequence, such as an undesirable side reaction as cleavage of peptide bond in the intermediate position of the peptide, and allows to carry out the chemical treatment thereof under widely applicable conditions. In the method according to the present invention, an alkanoic acid anhydride and a perfluoroalkanoic acid both of vapor phase, which are supplied from a mixture containing an alkanoic acid anhydride with a small amount of a perfluoroalkanoic acid added thereto, are allowed to act on a dry sample of the peptide to be examined in a dry atmosphere at a temperature chosen in a range of 15 to 60° C.; whereby the release of the C-terminal amino acid is resulted from successive formation of a 5-oxazolone structure being followed by cleavage of the 5-oxazolone ring; and then the C-terminal amino acids sequence is identified by analysis based on the decrease in molecular weight in a series of the reaction products obtained.
摘要翻译：本发明提供了通过应用从其中连续释放C末端氨基酸的反应技术来分析肽的C末端氨基酸序列的方法，当释放肽的C末端氨基酸时，该方法可以抑制序列，例如作为在肽的中间位置处的肽键的切割的不期望的副反应，并且允许在广泛适用的条件下进行其化学处理。在根据本发明的方法中，允许从含有链烷酸酐和少量全氟链烷酸的混合物中提供的气相的链烷酸酐和全氟烷酸作用于在干燥气氛中在15至60℃范围内选择的温度下待检测的肽的干样品; 由此C末端氨基酸的释放是由连续形成5-恶唑酮结构而引起的，随后是5-恶唑酮环的裂解; 然后通过基于所获得的一系列反应产物中分子量的降低的分析来鉴定C-末端氨基酸序列。

10. 发明授权

US08119411B2 Method for analyzing C-terminal amino acid sequence of peptide using mass spectrometry 失效
标题翻译：使用质谱分析肽的C-末端氨基酸序列的方法
公开(公告)号：US08119411B2
公开(公告)日：2012-02-21
申请号：US12973158
申请日：2010-12-20
申请人： Kenji Miyazaki , Akira Tsugita , Kenichi Kamijo , Hiroaki Torii
发明人： Kenji Miyazaki , Akira Tsugita , Kenichi Kamijo , Hiroaki Torii
IPC分类号： G01N33/00
CPC分类号： G01N33/6821 , G01N33/6842 , G01N33/6851
摘要： The present invention provides a method for analyzing the C-terminal amino acid sequence of a peptide by using a reaction for successively releasing the C-terminal amino acids of the peptide, which method can suppress, when successively releasing the C-terminal amino acids of a peptide of long amino acid length, such a undesirable side reaction as cleavage of peptide bond in the intermediate position of the peptide and can carry out the chemical treatment thereof under widely applicable conditions; In the method, a dry sample of a peptide with long amino acid length is beforehand subjected to an N-acylation treatment; by using a reaction reagent where an alkanoic acid anhydride is combined with a small amount of a perfluoroalkanoic acid, successive release of C-terminal amino acids is conducted under mild conditions; a hydrolysis treatment is applied; then, selective fragmentization at site of arginine residue is performed by digestion by trypsin; thereafter, decreases in molecular weight are measured for the C-terminal side fragments derived from a series of reaction products with use of a MALDI-TOF-MS apparatus; thereby, the C-terminal amino acid sequence of the peptide sample is identified.
摘要翻译：本发明提供了通过使用连续释放肽的C末端氨基酸的反应来分析肽的C末端氨基酸序列的方法，当连续释放C末端氨基酸的C末端氨基酸时，该方法可以抑制长氨基酸长度的肽，这种不利的副反应，如在肽的中间位置处的肽键断裂，并且可以在广泛适用的条件下进行化学处理; 在该方法中，将具有长氨基酸长度的肽的干样品预先进行N-酰化处理; 通过使用其中链烷酸酐与少量全氟链烷酸组合的反应试剂，在温和条件下连续释放C-末端氨基酸; 应用水解处理; 然后通过胰蛋白酶消化进行精氨酸残基位点的选择性碎裂; 此后，使用MALDI-TOF-MS装置测量衍生自一系列反应产物的C-末端侧片段的分子量的降低; 鉴定出肽样品的C-末端氨基酸序列。

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