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1. 发明授权

US08278427B2 Molecule for assigning genotype to phenotype and components thereof as well as method for constructing assigning molecule and method for utilizing assigning molecule 有权
标题翻译：用于将基因型分配给表型及其组分的分子以及用于构建分配分子的方法和用于分配分子的方法
公开(公告)号：US08278427B2
公开(公告)日：2012-10-02
申请号：US10460467
申请日：2003-06-13
申请人： Hiroshi Yanagawa , Etsuko Miyamoto , Hideaki Takashima
发明人： Hiroshi Yanagawa , Etsuko Miyamoto , Hideaki Takashima
IPC分类号： C07H21/04 , C12N15/00 , A61K31/70
CPC分类号： C12N15/1062
摘要： An assigning molecule obtained by ligating a genotype molecule to a phenotype molecule by transpeptidation, wherein the genotype molecule is constructed by bonding (a) a spacer molecule comprising a donor region which can be bonded to a 3′-terminal end of nucleic acid, a PEG region bonded to the donor region and a peptide acceptor region which is bonded to the PEG region, to (b) a 3′ end of a nucleic acid comprising a 5′ untranslated region comprising a transcription promoter and a translation enhancer; an ORF region which comprises a polyA sequence and which is bonded to the 3′-terminal side of the 5′ untranslated region; and a 3′-terminal region that is bonded to the 3′-terminal side of the ORF region.
摘要翻译：通过转基因将基因型分子连接到表型分子而获得的分配分子，其中所述基因型分子通过键合（a）包含可以键合到核酸的3'-末端的供体区的间隔分子，结合到供体区的PEG区和与PEG区结合的肽受体区，至（b）含有包含转录启动子和翻译增强子的5'非翻译区的核酸的3'末端; ORF区域，其包含polyA序列，并且其键合到5'非翻译区的3'末端侧; 和与ORF区域的3'末端侧键合的3'末端区域。

2. 发明授权

US07150978B2 Recombinant template used for producing a carboxy-terminal modified protien and a method of producing a carboxy-terminal modified protein 有权
标题翻译：用于制备羧基末端修饰的蛋白质的重组模板和产生羧基末端修饰蛋白质的方法
公开(公告)号：US07150978B2
公开(公告)日：2006-12-19
申请号：US10455453
申请日：2003-06-06
申请人： Hiroshi Yanagawa , Nobuhide Doi , Etsuko Miyamoto , Hideaki Takashima , Rieko Oyama
发明人： Hiroshi Yanagawa , Nobuhide Doi , Etsuko Miyamoto , Hideaki Takashima , Rieko Oyama
IPC分类号： C12P19/30
CPC分类号： C07K1/13 , C12P21/02
摘要： A translation template comprising an ORF region coding for a protein, a 5′ untranslated region comprising a transcription promoter and a translation enhancer and locating on the 5′ side of the ORF region, and a 3′ end region comprising a poly-A sequence and locating on the 3′ side of the ORF region, is expressed in a translation system in the presence of an agent for modifying a C-terminal of a protein, which comprises an acceptor portion having a group capable of binding to a protein through a transpeptidation reaction in a protein translation system and a modifying portion comprising a nonradioactive modifying substance linked to the acceptor portion via a nucleotide linker, to cause protein synthesis and the synthesized protein is purified. Thus, the yield of modified protein in a method of modifying C-terminal of protein is improved and detection of protein interaction based on various intermolecular interaction detection methods is realized at an improved level.
摘要翻译：翻译模板，其包含编码蛋白质的ORF区域，包含转录启动子的5'非翻译区和翻译增强子并且位于ORF区域的5'侧，以及包含poly-A序列的3'末端区域和位于ORF区域的3'侧的表达在翻译系统中，在存在用于修饰蛋白质C末端的试剂的情况下，其包含具有能够通过转肽酶结合蛋白质的基团的受体部分蛋白质翻译系统中的反应和包含通过核苷酸接头与受体部分连接的非放射性修饰物质的修饰部分进行蛋白质合成，合成的蛋白质被纯化。因此，改善蛋白质C-末端修饰方法中的修饰蛋白质的产率得到改善，基于各种分子间相互作用检测方法的蛋白质相互作用的检测得到提高。

3. 发明申请

US20050010028A1 C-terminal modified protein and method for producing the same, modifying agent and translation template used for producing C-terminal modified protein, and method for detecting protein interaction with use of C-terminal modified protein 有权
标题翻译： C-末端修饰蛋白及其制备方法，用于生产C-末端修饰蛋白的修饰剂和翻译模板，以及使用C末端修饰蛋白检测蛋白质相互作用的方法
公开(公告)号：US20050010028A1
公开(公告)日：2005-01-13
申请号：US10455453
申请日：2003-06-06
申请人： Hiroshi Yanagawa , Nobuhide Doi , Etsuko Miyamoto , Hideaki Takashima , Rieko Oyama
发明人： Hiroshi Yanagawa , Nobuhide Doi , Etsuko Miyamoto , Hideaki Takashima , Rieko Oyama
IPC分类号： C07K1/13 , C12P21/02 , C07K9/00 , A61K38/14
CPC分类号： C07K1/13 , C12P21/02
摘要： A translation template comprising an ORF region coding for a protein, a 5′ untranslated region comprising a transcription promoter and a translation enhancer and locating on the 5′ side of the ORF region, and a 3′ end region comprising a poly-A sequence and locating on the 3′ side of the ORF region, is expressed in a translation system in the presence of an agent for modifying a C-terminal of a protein, which comprises an acceptor portion having a group capable of binding to a protein through a transpeptidation reaction in a protein translation system and a modifying portion comprising a nonradioactive modifying substance linked to the acceptor portion via a nucleotide linker, to cause protein synthesis and the synthesized protein is purified. Thus, the yield of modified protein in a method of modifying C-terminal of protein is improved and detection of protein interaction based on various intermolecular interaction detection methods is realized at an improved level.
摘要翻译：翻译模板，其包含编码蛋白质的ORF区域，包含转录启动子的5'非翻译区和翻译增强子并且位于ORF区域的5'侧，以及包含poly-A序列的3'末端区域和位于ORF区域的3'侧的表达在翻译系统中，在存在用于修饰蛋白质C末端的试剂的情况下，其包含具有能够通过转肽酶结合蛋白质的基团的受体部分蛋白质翻译系统中的反应和包含通过核苷酸接头与受体部分连接的非放射性修饰物质的修饰部分进行蛋白质合成，合成的蛋白质被纯化。因此，改善蛋白质C-末端修饰方法中的修饰蛋白质的产率得到改善，基于各种分子间相互作用检测方法的蛋白质相互作用的检测得到提高。

4. 发明申请

US20060183885A1 Protein forming complex with c-fos protein, nucleic acid encoding the same and method of using the same 审中-公开
标题翻译：蛋白质与c-fos蛋白形成复合物，核酸编码相同，使用方法相同
公开(公告)号：US20060183885A1
公开(公告)日：2006-08-17
申请号：US10538410
申请日：2003-11-19
申请人： Etsuko Miyamoto , Masamichi Ishizaka , Hiroshi Yanagawa
发明人： Etsuko Miyamoto , Masamichi Ishizaka , Hiroshi Yanagawa
IPC分类号： C07K14/705 , C12Q1/68 , G01N33/53 , C07H21/04
CPC分类号： C12N15/1055 , C07K14/47 , G01N2500/04
摘要： Proteins that interact with c-Fos, nucleic acids encoding them and inhibitors utilizing them as well as methods for detecting an interaction and screening methods utilizing a protein that interacts with c-Fos are provided. Comprehensive analysis of transcription control factor complexes in a mouse brain cDNA library with c-Fos as a bait by using the cotranslation selection and screening of in vitro virus (IVV) and the C-terminal labeling method are conducted, thereby to analyze proteins unknown so far, proteins known so far, but unknown to form a complex with the c-Fos protein, and so forth.
摘要翻译：提供了与c-Fos相互作用的蛋白质，编码它们的核酸和利用它们的抑制剂以及利用与c-Fos相互作用的蛋白质的相互作用和筛选方法的方法。通过使用共转染选择和筛选体外病毒（IVV）和C末端标记方法，将c-Fos作为诱饵的小鼠脑cDNA文库中的转录控制因子复合物进行综合分析，从而分析蛋白质未知迄今为止已知的蛋白质，但未知与c-Fos蛋白质形成复合物等等。

5. 发明授权

US06361943B1 Molecule that homologizes genotype and phenotype and utilization thereof 有权
标题翻译：分子基因型和表型同源化及其利用
公开(公告)号：US06361943B1
公开(公告)日：2002-03-26
申请号：US09284627
申请日：1999-06-02
申请人： Hiroshi Yanagawa , Naoto Nemoto , Etsuko Miyamoto , Yuzuru Husimi
发明人： Hiroshi Yanagawa , Naoto Nemoto , Etsuko Miyamoto , Yuzuru Husimi
IPC分类号： C12Q168
CPC分类号： C12N15/11 , C12N15/1062
摘要： A molecule comprising a nucleic acid portion and a protein portion directly bound to said nucleic acid portion with a covalent bond, wherein said nucleic acid portion comprises a polymer of nucleoside, and said protein portion is encoded by said nucleic acid portion, and a method for constructing the molecule, which comprises (a) preparing a DNA containing a gene which has no termination codon, (b) transcribing the prepared DNA into RNA, (c) bonding a chimeric spacer composed of DNA and RNA to a 3′-terminal end of the obtained RNA, (d) bonding to a 3′-terminal end of the obtained bonded product, a nucleoside or a substance having a chemical structure analogous to that of a nucleoside, which can be covalently bound to an amino acid or substance having a chemical structure analogous to that of an amino acid, and (e) performing protein synthesis in a cell-free protein synthesis system using the obtained bonded product as mRNA to bond a nucleic acid portion containing the gene to a translation product of the gene. The molecule of the present invention is an extremely useful substance that can be used for evolutionary molecular engineering, i.e., modification of functional biopolymers such as enzymes, antibodies, and ribozymes, and creation of biopolymers having functions which cannot be found in living organisms.
摘要翻译：包含核酸部分和与所述核酸部分直接键合有共价键的蛋白质部分的分子，其中所述核酸部分包含核苷聚合物，所述蛋白质部分由所述核酸部分编码，构建分子，其包括（a）制备含有不具有终止密码子的基因的DNA，（b）将制备的DNA转录成RNA，（c）将由DNA和RNA组成的嵌合间隔物结合至3'末端的所得RNA，（d）与获得的结合产物的3'-末端结合，具有与核苷类似的化学结构的核苷或物质，其可共价结合于具有与氨基酸类似的化学结构，和（e）使用获得的结合产物作为mRNA将含有该基因的核酸部分键合到无转化蛋白质合成系统中进行蛋白质合成该基因的产物。本发明的分子是可用于进化分子工程，即功能性生物聚合物如酶，抗体和核酶的修饰以及具有在活生物体中不能发现的功能的生物聚合物的非常有用的物质。

6. 发明授权

US06228994B1 Labeled protein and its producing method, labeling compound to be used in the method, and method for analyzing function of genes 有权
公开(公告)号：US06228994B1
公开(公告)日：2001-05-08
申请号：US09190276
申请日：1998-11-13
申请人： Hiroshi Yanagawa , Naoto Nemoto , Etsuko Miyamoto
发明人： Hiroshi Yanagawa , Naoto Nemoto , Etsuko Miyamoto
IPC分类号： C07K113
CPC分类号： C07H19/20 , C07H19/04 , C07K1/13
摘要： A protein having a labeling compound attached to its C-terminal, in which the compound comprises a label portion comprising a label substance and an acceptor portion comprising a compound having an ability of binding to a C-terminal of a synthesized protein when protein synthesis is carried out in a cell-free protein synthesis system or in a living cell is provided. Also, a method for producing the protein comprising the step of performing synthesis of a protein in a cell-free protein synthesis system or in a living cell in the presence of a labeling compound comprising a label portion comprising a label substance and an acceptor portion comprising a compound having an ability of binding to a C-terminal of a synthesized protein when protein synthesis is carried out in the cell-free protein synthesis system or in the living cell, the labeling compound being present at a concentration effective for the labeling compound to bind to the C-terminal of the synthesized protein is provided. Also, the labeling compound and a method for analyzing a function of a gene are further provided.

7. 发明授权

US07838243B2 Protein forming complex with c-Jun protein, nucleic acid encoding the same and method of using the same 有权
标题翻译：蛋白质与c-Jun蛋白形成复合物，核酸编码相同，使用方法相同
公开(公告)号：US07838243B2
公开(公告)日：2010-11-23
申请号：US10579802
申请日：2004-11-19
申请人： Etsuko Miyamoto , Kenichi Horisawa , Hiroshi Yanagawa
发明人： Etsuko Miyamoto , Kenichi Horisawa , Hiroshi Yanagawa
IPC分类号： G01N33/53 , C12Q1/68
CPC分类号： C12N15/1055
摘要： Comprehensive analysis of transcription control factor complexes in a mouse brain cDNA library with c-Jun as a bait by using the cotranslation selection and screening of in vitro virus (IVV) and the C-terminal labeling method are conducted, thereby to provide known and unknown proteins which are unknown to form a complex with the c-Jun protein, whereby proteins that interact with c-Jun, nucleic acids encoding them and inhibitors utilizing them as well as methods for detecting an interaction and screening methods are provided.
摘要翻译：通过使用共转染选择和筛选体外病毒（IVV）和C末端标记方法，在c-Jun作为诱饵的小鼠脑cDNA文库中进行转录控制因子复合物的综合分析，从而提供已知和未知的与c-Jun蛋白形成复合物的未知蛋白质，提供了与c-Jun相互作用的蛋白质，编码它们的核酸和利用它们的抑制剂以及用于检测相互作用和筛选方法的方法。

8. 发明授权

US07670787B2 Protein forming complex with c-Fos protein, nucleic acid encoding the same and method of using the same 失效
标题翻译：蛋白质与c-Fos蛋白形成复合物，核酸编码相同，使用方法相同
公开(公告)号：US07670787B2
公开(公告)日：2010-03-02
申请号：US11976827
申请日：2007-10-29
申请人： Etsuko Miyamoto , Masamichi Ishizaka , Hiroshi Yanagawa
发明人： Etsuko Miyamoto , Masamichi Ishizaka , Hiroshi Yanagawa
IPC分类号： G01N33/53
CPC分类号： C12N15/1055 , C07K14/47 , G01N2500/04
摘要： Proteins that interact with c-Fos, nucleic acids encoding them and inhibitors utilizing them as well as methods for detecting an interaction and screening methods utilizing a protein that interacts with c-Fos are provided. Comprehensive analysis of transcription control factor complexes in a mouse brain cDNA library with c-Fos as a bait by using the cotranslation selection and screening of in vitro virus (IVV) and the C-terminal labeling method are conducted, thereby to analyze proteins unknown so far, proteins known so far, but unknown to form a complex with the c-Fos protein, and so forth.
摘要翻译：提供了与c-Fos相互作用的蛋白质，编码它们的核酸和利用它们的抑制剂以及利用与c-Fos相互作用的蛋白质的相互作用和筛选方法的方法。通过使用共转染选择和筛选体外病毒（IVV）和C末端标记方法，将c-Fos作为诱饵的小鼠脑cDNA文库中的转录控制因子复合物进行综合分析，从而分析蛋白质未知迄今为止已知的蛋白质，但未知与c-Fos蛋白质形成复合物等等。

9. 发明申请

US20080177030A1 Protein forming complex with c-Fos protein, nucleic acid encoding the same and method of using the same 失效
标题翻译：蛋白质与c-Fos蛋白形成复合物，核酸编码相同，使用方法相同
公开(公告)号：US20080177030A1
公开(公告)日：2008-07-24
申请号：US11976827
申请日：2007-10-29
申请人： Etsuko Miyamoto , Masamichi Ishizaka , Hiroshi Yanagawa
发明人： Etsuko Miyamoto , Masamichi Ishizaka , Hiroshi Yanagawa
IPC分类号： C07K14/47 , C07K14/00
CPC分类号： C12N15/1055 , C07K14/47 , G01N2500/04
摘要： Proteins that interact with c-Fos, nucleic acids encoding them and inhibitors utilizing them as well as methods for detecting an interaction and screening methods utilizing a protein that interacts with c-Fos are provided. Comprehensive analysis of transcription control factor complexes in a mouse brain cDNA library with c-Fos as a bait by using the cotranslation selection and screening of in vitro virus (IVV) and the C-terminal labeling method are conducted, thereby to analyze proteins unknown so far, proteins known so far, but unknown to form a complex with the c-Fos protein, and so forth.
摘要翻译：提供了与c-Fos相互作用的蛋白质，编码它们的核酸和利用它们的抑制剂以及利用与c-Fos相互作用的蛋白质的相互作用和筛选方法的方法。通过使用共转染选择和筛选体外病毒（IVV）和C末端标记方法，将c-Fos作为诱饵的小鼠脑cDNA文库中的转录控制因子复合物进行综合分析，从而分析蛋白质未知迄今为止已知的蛋白质，但未知与c-Fos蛋白质形成复合物等等。

10. 发明授权

US07041446B2 Labeled protein and its producing method, labeling compound to be used in the method, and method for analyzing function of genes 有权
标题翻译：标记蛋白及其制备方法，方法中使用的标记化合物和分析基因功能的方法
公开(公告)号：US07041446B2
公开(公告)日：2006-05-09
申请号：US09794128
申请日：2001-02-28
申请人： Hiroshi Yanagawa , Naoto Nemoto , Etsuko Miyamoto
发明人： Hiroshi Yanagawa , Naoto Nemoto , Etsuko Miyamoto
IPC分类号： C12Q1/68 , C07K1/13
CPC分类号： C07H19/20 , C07H19/04 , C07K1/13
摘要： A protein having a labeling compound attached to its C-terminal, in which the compound comprises a label portion comprising a label substance and an acceptor portion comprising a compound having an ability of binding to a C-terminal of a synthesized protein when protein synthesis is carried out in a cell-free protein synthesis system or in a living cell is provided. Also, a method for producing the protein comprising the step of performing synthesis of a protein in a cell-free protein synthesis system or in a living cell in the presence of a labeling compound comprising a label portion comprising a label substance and an acceptor portion comprising a compound having an ability of binding to a C-terminal of a synthesized protein when protein synthesis is carried out in the cell-free protein synthesis system or in the living cell, the labeling compound being present at a concentration effective for the labeling compound to bind to the C-terminal of the synthesized protein is provided. Also, the labeling compound and a method for analyzing a function of a gene are further provided.
摘要翻译：一种具有附着于其C-末端的标记化合物的蛋白质，其中所述化合物包含标记物部分，所述标记物包含标记物质和受体部分，所述受体部分包含当蛋白质合成为蛋白质合成时具有与合成蛋白质C末端结合的能力的化合物在无细胞蛋白质合成系统或活细胞中进行。另外，制备蛋白质的方法包括以下步骤：在标记化合物存在下，在无细胞蛋白质合成系统或活细胞中进行蛋白质的合成，所述标记化合物包含标记物质和受体部分，所述标记物包含当在无细胞蛋白质合成系统或活细胞中进行蛋白质合成时，具有与合成蛋白质C末端结合的能力的化合物，标记化合物以对标记化合物有效的浓度存在提供了与合成蛋白质的C-末端结合。此外，还提供了标记化合物和分析基因功能的方法。

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