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1. 发明申请

US20090269745A1 RNA EXTRACTION METHOD AND RNA DETECTION METHOD 审中-公开
标题翻译： RNA提取方法和RNA检测方法
公开(公告)号：US20090269745A1
公开(公告)日：2009-10-29
申请号：US12092067
申请日：2006-11-02
申请人： Hiroshi Tonoike , Yoshinari Shirasaki , Naoyuki Nishimura , Shigeru Tamatsukuri , Kuhomi Watanabe , Yasuhiko Sakakura , Hiroyuki Nakayama
发明人： Hiroshi Tonoike , Yoshinari Shirasaki , Naoyuki Nishimura , Shigeru Tamatsukuri , Kuhomi Watanabe , Yasuhiko Sakakura , Hiroyuki Nakayama
IPC分类号： C12Q1/68 , C12P19/34
CPC分类号： C12N15/1003 , C12Q1/6806 , C12Q2527/125 , C12Q2527/119 , C12Q2527/101
摘要： The present invention provides a method for inactivating RNase which generally presents in a sample such as biological sample (especially an excrement sample), or in a sample such as a living body-derived sample (especially an excrement-derived sample) obtained by separation of an RNA-including body therefrom or the like; a method for extracting and detecting RNA from the sample. An RNA extraction method, comprising the steps of: obtaining a mixture under a heating condition, said mixture comprising: a sample comprising an RNA-including body and RNase, and an alkaline treating reagent comprising at least a reducing agent, and having pH of 8.1 or higher, and conducting inactivation of the RNase and extraction of RNA from the RNA-including body by keeping the mixture under the heating condition. An RNA detection method, comprising conducting RNA amplification reaction by mixing a treated sample liquid comprising RNA extracted by the extraction method and an amplification reaction solution.
摘要翻译：本发明提供了通常存在于样品例如生物样品（特别是排泄物样品）中的RNase的灭活方法，或者通过分离获得的样品例如来自活体的样品（特别是排泄物衍生的样品）来自其的含RNA的体; 从样品中提取和检测RNA的方法。一种RNA提取方法，包括以下步骤：在加热条件下获得混合物，所述混合物包含：包含含RNA的体和RNase的样品和至少包含还原剂的碱处理试剂，pH为8.1 或更高，并且通过将混合物保持在加热条件下，使RNase失活并从含RNA的体内提取RNA。一种RNA检测方法，包括通过混合包含通过提取方法提取的RNA的经处理的样品液体和扩增反应溶液进行RNA扩增反应。

2. 发明授权

US06472187B1 Method for amplification of RNA 有权
标题翻译： RNA扩增方法
公开(公告)号：US06472187B1
公开(公告)日：2002-10-29
申请号：US09610901
申请日：2000-07-06
申请人： Hiroshi Tonoike , Naoyuki Nishimura
发明人： Hiroshi Tonoike , Naoyuki Nishimura
IPC分类号： C12P1934
CPC分类号： C12Q1/686 , C12Q2527/119 , C12Q2527/125 , C12Q2521/107
摘要： An object of the present invention is to establish a method for amplifying an RNA in a biologically-derived sample by preparing a reaction solution in which a nucleic acid synthesis is not inhibited even in the presence of various biologically-derived impurities and as well as to enable a convenient, rapid and highly sensitive analysis of the RNA in the sample. In a method of the present invention, a reaction solution having a pH higher than that of an ordinarily employed reaction solution and/or containing a polyamine and/or a sulfated polysaccharide.
摘要翻译：本发明的目的是建立一种通过制备其中核酸合成即使在各种生物衍生的杂质的存在下也不被抑制的反应溶液来扩增生物衍生的样品中的RNA的方法，以及使样品中RNA的方便，快速和高度敏感的分析。在本发明的方法中，具有pH高于通常使用的反应溶液和/或含有多胺和/或硫酸化多糖的pH的反应溶液。

3. 发明授权

US5720806A Filler for ink jet recording paper 失效
标题翻译：喷墨记录纸填料
公开(公告)号：US5720806A
公开(公告)日：1998-02-24
申请号：US722830
申请日：1996-09-27
申请人： Tsuneo Fujii , Hiroshi Tonoike , Daisaku Isomura
发明人： Tsuneo Fujii , Hiroshi Tonoike , Daisaku Isomura
IPC分类号： B41M5/00 , B41M5/50 , B41M5/52 , C09C1/30 , D21H19/38 , C08K5/00
CPC分类号： C09C1/30 , B41M5/52 , B41M5/5218 , C01P2002/02 , C01P2004/51 , C01P2004/61 , C01P2006/12 , C01P2006/16 , C01P2006/19 , C01P2006/22
摘要： The invention provides a filler for ink jet recording paper which is composed of an amorphous silica containing 0.3-1.0% by weight of Al.sub.2 O.sub.3 and having a BET specific surface area of 250-400 m.sup.2 /g, a mean particle size of 3.6-10.0 .mu.m and a peak position of pore radius as measured by mercury injection method of 37.5-75 angstrom. A coat layer of ink jet recording paper formed of this filler excels not only in true circle property of dots and ink absorbing property when printed by ink jet recording system, but also in surface strength and writing quality. Furthermore, the coating liquid containing this filler has a low viscosity and hence exhibits good coating workability.
摘要翻译：本发明提供一种喷墨记录纸用填料，其由含有0.3〜1.0重量％的Al 2 O 3的无定形二氧化硅构成，BET比表面积为250〜400m 2 / g，平均粒径为3.6〜10.0μm m和通过汞注入法测量的孔半径的峰值位置为37.5-75埃。由该填料形成的喷墨记录纸的涂层不仅在通过喷墨记录系统打印时不但具有点和油墨吸收性的真实圆特性，而且还具有表面强度和书写质量。此外，含有该填料的涂布液的粘度低，因此具有良好的涂布加工性。

4. 发明授权

US06767723B2 Method for synthesis of nucleic acids 有权
标题翻译：核酸的合成方法
公开(公告)号：US06767723B2
公开(公告)日：2004-07-27
申请号：US09818583
申请日：2001-03-28
申请人： Hiroshi Tonoike
发明人： Hiroshi Tonoike
IPC分类号： C12P1934
CPC分类号： C12Q1/6806 , C12Q2527/125
摘要： An object of the present invention is to provide a method of treatment and a method of storage that are useful in conducting a nucleic acid synthesis procedure capable of directly amplifying an intended nucleic acid in a living body-derived sample without purification steps. The present invention provides a method for synthesis of nucleic acids in which a living body-derived sample itself is mixed with a reaction solution for gene amplification and allowed to react, which method comprises treating the sample with a surfactant before the reaction to destruct solid components such as cells or bacterial bodies containing nucleic acids and uniformly disperse them in the sample liquid.
摘要翻译：本发明的目的是提供一种治疗方法和储存方法，其可用于进行能够直接扩增活体来源的样品中的目的核酸而不进行纯化步骤的核酸合成方法。本发明提供了一种用于合成核酸的方法，其中将活体衍生的样品本身与用于基因扩增的反应溶液混合并进行反应，该方法包括在反应之前用表面活性剂处理样品以破坏固体组分如细胞或含有核酸的细菌体并将其均匀地分散在样品液体中。

5. 发明授权

US06576447B2 Method for synthesis of nucleic acids 失效
标题翻译：核酸的合成方法
公开(公告)号：US06576447B2
公开(公告)日：2003-06-10
申请号：US09809219
申请日：2001-03-16
申请人： Hiroshi Tonoike
发明人： Hiroshi Tonoike
IPC分类号： C12P1934
CPC分类号： C12P19/34 , C12Q1/6806 , C12Q2527/125
摘要： An object of the present invention is to provide a novel method for suppressing the action of nucleic acid synthesis inhibitory substances and thereby amplifying a nucleic acid in a sample efficiently. According to the present invention, in a method for synthesis of nucleic acids to amplify an intended nucleic acid in a sample, the sample is brought in advance into contact with an insoluble polymer of a polyanion, a sulfated polymer or a sulfated polysaccharide, to remove nucleic acid synthesis inhibitory substances.
摘要翻译：本发明的目的是提供抑制核酸合成抑制物质的作用的新方法，从而有效地扩增样品中的核酸。根据本发明，在核酸的合成方法中，预先将样品中的核酸提前与聚阴离子，硫酸化聚合物或硫酸化多糖的不溶性聚合物接触以除去核酸合成抑制物质。

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