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1. 发明申请

US20130098764A1 METHOD FOR PRODUCING REACTION INSTRUMENT FOR ELECTROPHORESIS, APPARATUS FOR PRODUCING REACTION INSTRUMENT FOR ELECTROPHORESIS, BASE FOR GEL IMMOBILIZATION, REACTION INSTRUMENT FOR ELECTROPHORESIS AND KIT FOR ELECTROPHORESIS 审中-公开
标题翻译：用于生产电泳反应仪的方法，用于生产用于电泳的反应仪器，用于凝胶固定的基质，用于电泳的反应仪器和用于电泳的试剂盒的装置的方法
公开(公告)号：US20130098764A1
公开(公告)日：2013-04-25
申请号：US13704886
申请日：2011-01-12
申请人： Hiroshi Ohki , Yutaka Unuma , Yuji Maruo , Tsuyoshi Tanaka , Hiroshi Yamaki
发明人： Hiroshi Ohki , Yutaka Unuma , Yuji Maruo , Tsuyoshi Tanaka , Hiroshi Yamaki
IPC分类号： G01N27/447
CPC分类号： G01N27/44795 , G01N27/44747
摘要： A production method of the present invention is a method of producing a reaction instrument for electrophoresis (10), which is constituted by (i) a substrate (1) and (ii) an electrophoresis gel (3) which is immobilized on the substrate (1), including the steps of: (i) discharging a liquid to a surface of the substrate (1) on which surface the gel is to be immobilized, thereby forming a liquid pool (5); and thereafter (ii) discharging a gel solution to the liquid pool (5). This provides a method of producing a reaction instrument for electrophoresis, which method makes it possible to form a gel with high reliability in high yield.
摘要翻译：本发明的制造方法是制造电泳用反应装置（10）的方法，其由（i）基板（1）和（ii）固定在基板上的电泳凝胶（3）构成 1），包括以下步骤：（i）将液体排出到要固定凝胶表面的基底（1）的表面，从而形成液体池（5）; （ii）将凝胶溶液排出到液池（5）。这提供了一种生产电泳反应仪器的方法，该方法使得可以以高产率形成高可靠性的凝胶。

2. 发明申请

US20140076727A1 ELECTROPHORESIS-GEL-FORMING MONOMER SOLUTION, SOLUTION EJECTING METHOD, METHOD FOR FORMING ELECTROPHORESIS GEL, ELECTROPHORESIS GEL, AND ELECTROPHORESIS REACTION INSTRUMENT 审中-公开
标题翻译：电泳 - 凝胶成型单体溶液，溶液喷射方法，形成电泳凝胶的方法，电泳凝胶和电泳反应仪
公开(公告)号：US20140076727A1
公开(公告)日：2014-03-20
申请号：US14007748
申请日：2012-03-27
申请人： Hiroshi Ohki , Hiroshi Yamaki , Yutaka Unuma , Yuji Maruo , Tsuyoshi Tanaka
发明人： Hiroshi Ohki , Hiroshi Yamaki , Yutaka Unuma , Yuji Maruo , Tsuyoshi Tanaka
IPC分类号： G01N27/447
CPC分类号： G01N27/447 , G01N27/44747
摘要： A solution ejecting method includes a first ejection step of ejecting an electrophoresis-gel-forming monomer solution containing at least a monomer that forms a gel structure and a gel polymerization accelerator that activates a gel polymerization initiator and a second ejection step of ejecting a gel polymerization initiating solution containing the gel polymerization initiator onto the electrophoresis-gel-forming monomer solution.
摘要翻译：溶液喷射方法包括：第一喷射步骤，喷射至少含有形成凝胶结构的单体的电泳 - 凝胶形成单体溶液和激活凝胶聚合引发剂的凝胶聚合促进剂和喷射凝胶聚合的第二喷射步骤将引发含有凝胶聚合引发剂的溶液引入电泳 - 凝胶形成单体溶液。

3. 发明申请

US20140224657A1 SAMPLE SEPARATION AND ADSORPTION APPLIANCE 有权
标题翻译：样品分离和吸附装置
公开(公告)号：US20140224657A1
公开(公告)日：2014-08-14
申请号：US14343528
申请日：2011-09-12
申请人： Kouji Kusumoto , Yutaka Unuma , Hideki Kinoshita , Shinichi Goto , Tsuyoshi Tanaka , Yuji Maruo
发明人： Kouji Kusumoto , Yutaka Unuma , Hideki Kinoshita , Shinichi Goto , Tsuyoshi Tanaka , Yuji Maruo
IPC分类号： G01N27/447
CPC分类号： G01N27/44773 , G01N27/44739
摘要： A sample separation and adsorption appliance (100) includes a negative electrode (2), a positive electrode (3), a sample separation unit (6) that has a first opening (17) opened to a side facing the negative electrode (2) and a second opening (18) opened to a side facing the positive electrode (3), the sample separation unit containing a separation gel (7), and a slit structure (8) including a slit (1) at a position facing the second opening (18). A transfer film (9) is arranged between the second opening (18) and the slit (1).
摘要翻译：样品分离吸附装置（100）包括负极（2），正极（3），样品分离单元（6），其具有朝向负极（2）的一侧开口的第一开口（17）以及向与所述正极（3）相对的一侧开放的第二开口（18），所述样品分离单元包含分离凝胶（7），以及狭缝结构（8），所述狭缝结构（8）在面向所述第二开（18）。转印膜（9）布置在第二开口（18）和狭缝（1）之间。

4. 发明申请

US20140110261A1 CHIP FOR ELECTROPHORESIS AND METHOD FOR PRODUCING SAME 审中-公开
标题翻译：电泳用芯片及其制造方法
公开(公告)号：US20140110261A1
公开(公告)日：2014-04-24
申请号：US14127614
申请日：2012-06-19
申请人： Hiroshi Ohki , Yuji Maruo , Yutaka Unuma
发明人： Hiroshi Ohki , Yuji Maruo , Yutaka Unuma
IPC分类号： B01D57/02 , B05D1/36
CPC分类号： B01D57/02 , B05D1/36 , G01N27/44747
摘要： A chip (10) for electrophoresis is provided, the chip including a gel (9) composed of a polymer prepared by polymerizing a monomer, and a support (1) configured to support the gel (9). In the chip (10) for electrophoresis, a surface of the support (1) in contact with the gel (9) is covered with a surface treatment compound (4) containing the monomer or a derivative of the monomer.
摘要翻译：提供了用于电泳的芯片（10），该芯片包括由通过聚合单体制备的聚合物构成的凝胶（9）和构造成支持凝胶（9）的载体（1）。在用于电泳的芯片（10）中，与含有单体或单体衍生物的表面处理化合物（4）覆盖与凝胶（9）接触的载体（1）的表面。

5. 发明授权

US09103781B2 Sample separation and adsorption appliance 有权
标题翻译：样品分离和吸附装置
公开(公告)号：US09103781B2
公开(公告)日：2015-08-11
申请号：US14343528
申请日：2011-09-12
申请人： Kouji Kusumoto , Yutaka Unuma , Hideki Kinoshita , Shinichi Goto , Tsuyoshi Tanaka , Yuji Maruo
发明人： Kouji Kusumoto , Yutaka Unuma , Hideki Kinoshita , Shinichi Goto , Tsuyoshi Tanaka , Yuji Maruo
IPC分类号： G01N27/447
CPC分类号： G01N27/44773 , G01N27/44739
摘要： A sample separation and adsorption appliance (100) includes a negative electrode (2), a positive electrode (3), a sample separation unit (6) that has a first opening (17) opened to a side facing the negative electrode (2) and a second opening (18) opened to a side facing the positive electrode (3), the sample separation unit containing a separation gel (7), and a slit structure (8) including a slit (1) at a position facing the second opening (18). A transfer film (9) is arranged between the second opening (18) and the slit (1).
摘要翻译：样品分离吸附装置（100）包括负极（2），正极（3），样品分离单元（6），其具有朝向负极（2）的一侧开口的第一开口（17）以及向与所述正极（3）相对的一侧开放的第二开口（18），所述样品分离单元包含分离凝胶（7），以及狭缝结构（8），所述狭缝结构（8）在面向所述第二开（18）。转印膜（9）布置在第二开口（18）和狭缝（1）之间。

6. 发明申请

US20110114485A1 ELECTROPHORESIS APPARATUS AND ELECTROPHORESIS METHOD 有权
标题翻译：电泳装置和电泳方法
公开(公告)号：US20110114485A1
公开(公告)日：2011-05-19
申请号：US13054271
申请日：2009-07-15
申请人： Koji Sakairi , Satonari Akutsu , Yuji Maruo , Takateru Matsunaga , Yutaka Unuma , Atsunori Hiratsuka , Hideki Kinoshita , Nao Sakaguchi , Kenji Yokoyama
发明人： Koji Sakairi , Satonari Akutsu , Yuji Maruo , Takateru Matsunaga , Yutaka Unuma , Atsunori Hiratsuka , Hideki Kinoshita , Nao Sakaguchi , Kenji Yokoyama
IPC分类号： G01N27/447 , G01N27/453
CPC分类号： G01N27/44773
摘要： An electrophoresis apparatus includes a sample separating section for containing a sample separating medium for separating a sample in a horizontal direction, the sample separating section containing the sample separating medium in such a manner that the sample separating medium has an exposed portion at least one end of a surface of the sample separating medium, the surface being in parallel with the horizontal direction; and medium connecting means for connecting a sample containing medium to the sample separating medium at a connecting region, the sample containing medium containing a sample, and the connecting region satisfying a particular equation. By this, an electrophoresis technique with better accuracy than conventional arts can be provided.
摘要翻译：电泳装置包括样品分离部分，用于容纳用于在水平方向上分离样品的样品分离介质，样品分离部分包含样品分离介质，使得样品分离介质具有暴露部分，至少一端所述样品分离介质的表面与所述水平方向平行; 以及介质连接装置，用于在连接区域连接包含样品的介质到样品分离介质，所述样品包含含有样品的介质和满足特定等式的连接区域。由此，可以提供比现有技术更好的精度的电泳技术。

7. 发明授权

US08702950B2 Device for electrophoresis, device for transfer, device for electrophoresis and transfer, chip for electrophoresis and transfer, and method for electrophoresis, method for transfer, and method for electrophoresis and transfer 失效
标题翻译：电泳装置，转移装置，电泳和转印装置，电泳和转印用芯片，电泳方法，转印方法及电泳转印方法
公开(公告)号：US08702950B2
公开(公告)日：2014-04-22
申请号：US13607844
申请日：2012-09-10
申请人： Yuji Maruo , Yutaka Unuma , Atsunori Hiratsuka , Hideki Kinoshita , Kenji Yokoyama , Koji Sakairi , Satonari Akutsu
发明人： Yuji Maruo , Yutaka Unuma , Atsunori Hiratsuka , Hideki Kinoshita , Kenji Yokoyama , Koji Sakairi , Satonari Akutsu
IPC分类号： G01N27/26 , B01D57/02
CPC分类号： G01N27/44704 , B01L3/50273
摘要： A device for electrophoresis applies a voltage to a medium in contact with a plurality of electric conductors so that a potential of adjacent conductors is within a certain range. This allows preventing decline in electrophoresis speed. A device for electrophoresis and transfer includes an electrode having a plurality of electrode regions being insulated one another and arranged in a specific direction. This allows providing a practical and easy-to-use device for electrophoresis and transfer. A device for transfer alters an applied voltage or applied voltage duration to a certain position to another position. This allows improving transfer efficiency.
摘要翻译：用于电泳的装置将电压施加到与多个电导体接触的介质，使得相邻导体的电位在一定范围内。这样可以防止电泳速度下降。用于电泳和转印的装置包括具有彼此绝缘并沿特定方向布置的多个电极区域的电极。这允许提供用于电泳和转移的实用且易于使用的装置。用于传送的装置将施加的电压或施加的电压持续时间改变到某个位置到另一位置。这样可以提高传输效率。

8. 发明授权

US07964077B2 Automated two-dimensional electrophoresis apparatus and instrument constituting the apparatus 有权
标题翻译：构成装置的自动二维电泳装置和仪器
公开(公告)号：US07964077B2
公开(公告)日：2011-06-21
申请号：US11510838
申请日：2006-08-28
申请人： Yuji Maruo , Katsuyoshi Takahashi , Yutaka Unuma , Keisuke Usui , Hideki Kinoshita , Kisho Shiseki , Yoshio Suzuki , Atsunori Hiratsuka , Hiroyuki Fukui , Kenji Yokoyama , Ichiji Namatame , Kouhei Yodoya , Yasuhiro Ogawa , Koji Sakairi , Chie Hayashida , Satonari Akutsu , Kazuyoshi Yano , Isao Karube
发明人： Yuji Maruo , Katsuyoshi Takahashi , Yutaka Unuma , Keisuke Usui , Hideki Kinoshita , Kisho Shiseki , Yoshio Suzuki , Atsunori Hiratsuka , Hiroyuki Fukui , Kenji Yokoyama , Ichiji Namatame , Kouhei Yodoya , Yasuhiro Ogawa , Koji Sakairi , Chie Hayashida , Satonari Akutsu , Kazuyoshi Yano , Isao Karube
IPC分类号： G01N27/26 , G01N27/447
CPC分类号： G01N27/44778
摘要： The present invention is to provide a sample separation instrument used in a sample separation apparatus which includes: holding means for holding a first medium supporter which supports a first medium; and driving means for moving fixing means or the holding means in a direction parallel or perpendicular to a plane whose sides extend in the first direction and in the second directions. The sample separation instrument includes an insulator for storing a second medium which allows a sample separated in the first medium in the first direction to be further separated in the second direction different from the first direction, wherein: the insulator includes a first opening and a second opening each of which defines the second direction in which the second medium is electrified, and the second opening has a shape which allows the first medium containing the separated sample to be attached to the second medium along the first direction. The present invention realizes automation of the two-dimensional electrophoresis to enhance the convenience of the two-dimensional electrophoresis, to less depend on the operator's skill, and to enhance the reproducibility of the electrophoresis result.
摘要翻译：本发明提供一种在样品分离装置中使用的样品分离装置，其包括：保持装置，用于保持支撑第一介质的第一介质支持体; 以及用于在平行或垂直于其侧面沿第一方向和第二方向延伸的平面的方向上移动固定装置或保持装置的驱动装置。样品分离装置包括用于存储第二介质的绝缘体，其允许在第一方向上在第一介质中分离的样品在与第一方向不同的第二方向上进一步分离，其中：绝缘体包括第一开口和第二开口其中每个开口限定第二介质带电的第二方向，第二开口具有允许包含分离样品的第一介质沿着第一方向附着到第二介质的形状。本发明实现了二维电泳的自动化，以增强二维电泳的便利性，较少依赖于操作者的技能，并提高电泳结果的再现性。

9. 发明申请

US20070278102A1 Electrophoresis Apparatus and Device Therefor 有权
标题翻译：电泳装置及其装置
公开(公告)号：US20070278102A1
公开(公告)日：2007-12-06
申请号：US11663646
申请日：2006-09-05
申请人： Chie Hayashida , Koji Sakairi , Katsuyoshi Takahashi , Yuji Maruo , Michinobu Mieda , Yutaka Unuma
发明人： Chie Hayashida , Koji Sakairi , Katsuyoshi Takahashi , Yuji Maruo , Michinobu Mieda , Yutaka Unuma
IPC分类号： G01N27/447 , B01D57/02 , G01N21/64
CPC分类号： G01N27/44704 , B01D57/02 , G01N21/6428
摘要： There is provided an electrophoresis device (100) including an insulator (10) that includes: a first-separating-medium storing section (4′) for storing therein a first separating medium (4); a first opening (7) and a second opening (8) that are in communication with the first-separating-medium storing section (4′) and for defining a direction of separation on the first separating medium (4); and a third opening (9) that is covered with a penetrable insulating thin film (3), wherein the first separating medium storing section (4′) is in communication with outside via the first opening (7) and the second opening (8). There is also provided an electrophoresis apparatus including the electrophoresis device (100). This realizes an electrophoresis apparatus, and a device therefor, that enables an operator to sample separated proteins without ever making contact with the electrophoresed gel, and that can easily be used with various types of analyses, thereby improving convenience of electrophoresis.
摘要翻译：提供了一种包括绝缘体（10）的电泳装置（100），其包括：用于在其中存储第一分离介质（4）的第一分离介质存储部分（4'）; 第一开口（7）和第二开口（8），其与第一分离介质存储部分（4'）连通并且用于限定第一分离介质（4）上的分离方向; 和覆盖有可穿透绝缘薄膜（3）的第三开口（9），其中第一分离介质容纳部分（4'）经由第一开口（7）和第二开口（8）与外部连通，。还提供了包括电泳装置（100）的电泳装置。这实现了一种电泳装置及其装置，其使得操作者能够对分离的蛋白质进行取样，而不会与电泳凝胶接触，并且可以容易地与各种类型的分析一起使用，从而提高电泳的方便性。

10. 发明申请

US20120273360A1 INSTRUMENT FOR ELECTROPHORESIS AND ELECTROPHORESIS APPARATUS 审中-公开
标题翻译：电泳和电泳装置仪器
公开(公告)号：US20120273360A1
公开(公告)日：2012-11-01
申请号：US13518686
申请日：2010-12-21
申请人： Hideki Kinoshita , Yuji Maruo , Yutaka Unuma , Atsunori Hiratsuka , Koji Sakairi , Satonari Akutsu
发明人： Hideki Kinoshita , Yuji Maruo , Yutaka Unuma , Atsunori Hiratsuka , Koji Sakairi , Satonari Akutsu
IPC分类号： C25B9/00 , G01N27/447
CPC分类号： G01N27/44756 , G01N27/44743
摘要： An instrument for electrophoresis includes a sample-separating portion in which a slab sample-separating medium that separates a sample is provided. The instrument for electrophoresis includes a sample-transporting portion that transports a sample-containing medium containing the sample to the sample-separating portion. The instrument for electrophoresis includes a positioning portion that is provided to the sample-separating portion and/or the sample-transporting portion and used for connecting the sample-transporting portion to a predetermined position of the sample-separating portion.
摘要翻译：电子仪器包括样品分离部分，其中提供分离样品的板坯样品分离介质。用于电泳的仪器包括将含有样品的含样本培养基输送到样品分离部分的样品输送部分。电泳仪包括设置在样品分离部分和/或样品输送部分上的定位部分，用于将样品输送部分连接到样品分离部分的预定位置。

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