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1. 发明授权

US07824855B2 Method for selectively separating and purifying RNA and method for separating and purifying nucleic acid 有权
标题翻译：选择性分离和纯化RNA的方法和分离和纯化核酸的方法
公开(公告)号：US07824855B2
公开(公告)日：2010-11-02
申请号：US10593183
申请日：2005-03-25
申请人： Hiroko Inomata , Rie Iwata
发明人： Hiroko Inomata , Rie Iwata
IPC分类号： C12Q1/68 , C07H21/00 , C07H21/02 , C07H21/04
CPC分类号： C12N15/1017 , B01D15/08
摘要： A method for selectively separating and purifying RNA from a mixture solution of nucleic acid containing DNA and RNA, wherein the method comprising the steps of: (1-a) adsorbing nucleic acid; (1-b) washing; (1-c) subjecting to a DNase treatment; (1-d) washing; and (1-e) desorbing the RNA from a nucleic acid-adsorbing porous membrane by a recovering solution, wherein in the step (1-c), a total amount of a DNase solution is 130 μl or less per 1 cm2 of the membrane. And a method for selectively separating and purifying RNA or DNA, which comprises the steps of: (2-a) adsorbing nucleic acid; (2-b) washing by a washing solution; and (2-c) desorbing the nucleic acid from a nucleic acid-adsorbing porous membrane, wherein the washing solution contains a water-soluble organic solvent having a concentration of 50% by weight or less, and does not contain a chaotropic salt.
摘要翻译：一种用于从含有DNA和RNA的核酸的混合溶液中选择性分离和纯化RNA的方法，其中所述方法包括以下步骤：（1-a）吸附核酸; （1-b）洗涤; （1-c）进行DNA酶处理; （1-d）洗涤; 和（1-e）通过回收溶液从核酸吸附多孔膜解吸RNA，其中在步骤（1-c）中，每1cm 2膜的DNA酶溶液的总量为130μl以下。以及选择性分离和纯化RNA或DNA的方法，包括以下步骤：（2-a）吸附核酸; （2-b）用洗涤液洗涤; 和（2-c）从核酸吸附多孔膜解吸所述核酸，其中所述洗涤液含有浓度为50重量％以下的水溶性有机溶剂，并且不含离液序列。

2. 发明申请

US20080248559A1 Method For Selectively Separating and Purifying Rna and Method For Separating and Purifying Nucleic Acid 有权
标题翻译：选择性分离和纯化Rna的方法和分离和纯化核酸的方法
公开(公告)号：US20080248559A1
公开(公告)日：2008-10-09
申请号：US10593183
申请日：2005-03-25
申请人： Hiroko Inomata , Rie Iwata
发明人： Hiroko Inomata , Rie Iwata
IPC分类号： C12N1/08 , C07H1/06 , C12M1/00
CPC分类号： C12N15/1017 , B01D15/08
摘要： A method for selectively separating and purifying RNA from a mixture solution of nucleic acid containing DNA and RNA, wherein the method comprising the steps of: (1-a) adsorbing nucleic acid; (1-b) washing; (1-c) subjecting to a DNase treatment; (1-d) washing; and (1-e) desorbing the RNA from a nucleic acid-adsorbing porous membrane by a recovering solution, wherein in the step (1-c), a total amount of a DNase solution is 130 μl or less per 1 cm2 of the membrane. And a method for selectively separating and purifying RNA or DNA, which comprises the steps of: (2-a) adsorbing nucleic acid; (2-b) washing by a washing solution; and (2-c) desorbing the nucleic acid from a nucleic acid-adsorbing porous membrane, wherein the washing solution contains a water-soluble organic solvent having a concentration of 50% by weight or less, and does not contain a chaotropic salt.
摘要翻译：一种用于从含有DNA和RNA的核酸的混合溶液中选择性分离和纯化RNA的方法，其中所述方法包括以下步骤：（1-a）吸附核酸; （1-b）洗涤; （1-c）进行DNA酶处理; （1-d）洗涤; 和（1-e）通过回收溶液从核酸吸附多孔膜解吸RNA，其中在步骤（1-c）中，DNase溶液的总量为每1cm 2 130ul或更少， 2的膜。以及选择性分离和纯化RNA或DNA的方法，包括以下步骤：（2-a）吸附核酸; （2-b）用洗涤液洗涤; 和（2-c）从核酸吸附多孔膜解吸核酸，其中洗涤液含有浓度为50重量％以下的水溶性有机溶剂，不含有离液序列。

3. 发明申请

US20080113356A1 Method of Separating and Purifying Nucleic Acid 审中-公开
标题翻译：分离和纯化核酸的方法
公开(公告)号：US20080113356A1
公开(公告)日：2008-05-15
申请号：US11794426
申请日：2006-02-02
申请人： Tasuku Sasaki , Hiroko Inomata , Rie Iwata
发明人： Tasuku Sasaki , Hiroko Inomata , Rie Iwata
IPC分类号： C12Q1/68 , C12N1/08 , C07H21/04
CPC分类号： C12N15/1006
摘要： A method for separating and purifying a nucleic acid comprising steps of: (1) adding a lysis solution to a biomaterial to prepare a sample solution containing a nucleic acid, and adding a water-soluble organic solvent or a solution containing a water-soluble organic solvent to the sample solution thereby preparing a sample solution containing the water-soluble organic solvent; (2) contacting the sample solution containing the water-soluble organic solvent with a solid phase thereby adsorbing the nucleic acid on the solid phase; (3) contacting a washing solution with the solid phase thereby washing the solid phase in a state where the nucleic acid is adsorbed on the solid phase; and (4) contacting a recovering solution with the solid phase thereby desorbing the nucleic acid from the solid phase, wherein, in the step (1), the water-soluble organic solvent or the solution containing the water-soluble organic solvent is added separately in at least two batches.
摘要翻译：一种分离和纯化核酸的方法，包括以下步骤：（1）向生物材料中加入裂解溶液以制备含有核酸的样品溶液，并加入水溶性有机溶剂或含有水溶性有机溶剂溶剂，从而制备含有水溶性有机溶剂的样品溶液; （2）使含有水溶性有机溶剂的样品溶液与固相接触，从而将核酸吸附在固相上; （3）使洗涤液与固相接触，从而在核酸被吸附在固相上的状态下洗涤固相; 和（4）使回收溶液与固相接触，从而从固相中解吸核酸，其中在步骤（1）中，分别加入水溶性有机溶剂或含有水溶性有机溶剂的溶液至少两批。

4. 发明申请

US20070175826A1 Method for separating and purifying nucleic acid 审中-公开
标题翻译：核酸分离纯化方法
公开(公告)号：US20070175826A1
公开(公告)日：2007-08-02
申请号：US10593270
申请日：2005-03-18
申请人： Rie Iwata , Yoshihiko Makino
发明人： Rie Iwata , Yoshihiko Makino
IPC分类号： B01D61/24
CPC分类号： C07H21/00 , C12N15/1017 , Y10T436/255
摘要： A method for separating and purifying a nucleic acid, which comprises: (1) adsorbing the nucleic acid to a nucleic acid adsorbing porous membrane by passing a sample solution containing the nucleic acid through the nucleic acid adsorbing porous membrane; (2) washing the nucleic acid adsorbing porous membrane by passing a washing solution through the nucleic acid adsorbing porous membrane, while the nucleic acid is adsorbed to the nucleic acid adsorbing porous membrane; and (3) desorbing the nucleic acid from the nucleic acid adsorbing porous membrane by passing a recovering solution through the nucleic acid adsorbing porous membrane, wherein the nucleic acid adsorbing porous membrane is a porous membrane that has a contact angle of 60 degree or less after 17 m seconds of contact of the porous membrane with 3 μl of water dropped to the porous membrane.
摘要翻译：一种分离和纯化核酸的方法，其包括：（1）通过使含有核酸的样品溶液通过核酸吸附多孔膜将核酸吸附到吸附多孔膜的核酸上; （2）在核酸吸附于核酸吸附多孔膜的同时，通过使洗涤液通过核酸吸附多孔膜来洗涤核酸吸附多孔膜; （3）通过使回收溶液通过核酸吸附多孔膜从吸附多孔膜的核酸解吸核酸，其中，所述核酸吸附多孔膜是在多孔膜之后接触角为60度以下的多孔膜，多孔膜与3微摩尔水的接触17分钟滴加到多孔膜上。

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