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1. 发明授权

US4465772A Method for disinfecting and washing of immobilized lactase 失效
标题翻译：消毒和洗涤固定化乳糖酶的方法
公开(公告)号：US4465772A
公开(公告)日：1984-08-14
申请号：US307578
申请日：1981-10-01
申请人： Hideo Hirohara , Hidefumi Yamamoto , Emiko Kawano , Mamoru Hattori , Hisao Yamaguchi
发明人： Hideo Hirohara , Hidefumi Yamamoto , Emiko Kawano , Mamoru Hattori , Hisao Yamaguchi
IPC分类号： A23C21/02 , C12N9/38 , C12N11/00 , C12N11/02 , C12N11/08 , C12P1/00 , C12P19/02
CPC分类号： A23C21/023 , C12N11/08 , C12N9/2402 , C12N9/2471 , C12N9/86 , C12P1/00 , C12Y302/01023 , C12Y302/01108 , C12Y305/02006 , Y10S435/80
摘要： Effective disinfection and washing of immobilized lactase from Aspergillus oryzae is carried out after a period of hydrolyzing lactose by treatment of the immobilized lactase with aqueous solutions of pH's 2 to 4 and 6 to 8.7, one or both of which contain a disinfectant. The solution having a pH of 6 to 8.7 may be a phosphate buffered solution. The lactase may be immobilized on a high polymer compound by covalent linkage. The immobilized lactase has outstanding resistance to chemicals and wide pH stability so that decrease of lactase activity is minimized.
摘要翻译：通过用pH为2至4和6至8.7的水溶液处理固定化的乳糖酶，在一段时间内水解乳糖后，从米曲霉中有效消毒和洗涤固定化的乳糖酶，其中一种或两种含有消毒剂。 pH为6至8.7的溶液可以是磷酸盐缓冲溶液。可通过共价连接将乳糖酶固定在高分子化合物上。固定化的乳糖酶具有优异的耐化学性和宽的pH稳定性，使得乳糖酶活性的降低最小化。

2. 发明授权

US4239854A Enzyme-immobilization carriers and preparation thereof 失效
标题翻译：酶固定载体及其制备
公开(公告)号：US4239854A
公开(公告)日：1980-12-16
申请号：US899466
申请日：1978-04-24
申请人： Hideo Hirohara , Shigeyasu Nabeshima , Tsuneyuki Nagase
发明人： Hideo Hirohara , Shigeyasu Nabeshima , Tsuneyuki Nagase
IPC分类号： B01J43/00 , C12N11/08 , C12P13/12 , C12P19/14 , C12P19/24 , C08G2/00
CPC分类号： C12P13/12 , B01J43/00 , C12N11/08 , C12P19/14 , C12P19/24
摘要： Enzyme-immobilization carriers which comprise a macroporous amphoteric ion-exchange resin having a cation-exchange capacity due to carboxymethyl groups of 0.5 meq/g-dry resin or more, an anion-exchange capacity of 1 meq/g-dry resin or more, and a specific surface area of 1 m.sup.2 /g-dry resin or more, the total volume of macropores having a diameter of 100 A to 2000 A being 0.1 cc/g-dry resin or more, and a process for producing said carriers.
摘要翻译：包含由羧甲基为0.5meq / g-干树脂或更多阳离子交换容量，1meq / g-干树脂或更多的阴离子交换容量而具有阳离子交换能力的大孔性两性离子交换树脂的酶固定载体，比表面积为1m 2 / g-干树脂或更高，直径为100A至2000A的大孔的总体积为0.1cc / g-干树脂或更高，以及用于生产所述载体的方法。

3. 发明授权

US4607013A Biochemical process for optical resolution of cyclopentenolone derivatives 失效
标题翻译：环戊烯醇衍生物的光学拆分生化工艺
公开(公告)号：US4607013A
公开(公告)日：1986-08-19
申请号：US646809
申请日：1984-09-07
申请人： Satoshi Mitsuda , Hideo Hirohara
发明人： Satoshi Mitsuda , Hideo Hirohara
IPC分类号： C12P7/26 , C12P41/00 , C12P7/38
CPC分类号： C12P41/004 , C12P7/26
摘要： A biochemical process for optical resolution of 4-hydroxy-3-methyl-2-2'-propynyl-2-cyclopentenone, which comprises asymmetrically hydrolyzing organic carboxylic acid (saturated or unsaturated carboxylic acids having 1 to 18 carbon atoms) esters of (.+-.)-4-hydroxy-3-methyl-2-2'-propynyl-2-cyclopentenone by the action of an esterase originated from a microorganism or an animal pancreas to give optically active 4-hydroxy-3-methyl-2-2'-propynyl-2-cyclopentenone and esters of its antipode with high optical purity is disclosed. This resolution process is simple in steps as compared with the conventionally known organic chemical methods of optical resolution, and is quite advantageous industrially because it does not require expensive optically active reagents.
摘要翻译： PCT No.PCT / JP83 / 00086 Sec。 371日期：1984年9月7日 102（e）日期1984年9月7日PCT提交1983年3月18日PCT公布。出版物WO84 / 03714 日期：1984年9月27日。用于光学拆分4-羟基-3-甲基-2,2'-丙炔基-2-环戊烯酮的生物化学方法，其包括不对称水解有机羧酸（具有1至18个碳原子的饱和或不饱和羧酸碳原子）（+/-） - 4-羟基-3-甲基-2,2'-丙炔-2-环戊烯酮的酯通过起源于微生物或动物胰腺的酯酶的作用，得到光学活性的4-羟基公开了3-甲基-2,2'-丙炔基-2-环戊烯酮及其具有高光学纯度的对映体的酯。与常规已知的光学拆分的有机化学方法相比，该拆分方法的步骤简单，并且在工业上相当有利，因为它不需要昂贵的光学活性试剂。

4. 发明授权

US4108723A Method for optical resolution of DL-lysine compounds 失效
标题翻译： DL-赖氨酸化合物的光学拆分方法
公开(公告)号：US4108723A
公开(公告)日：1978-08-22
申请号：US829945
申请日：1977-09-01
申请人： Hideo Hirohara , Shigeyasu Nabeshima , Tsuneyuki Nagase
发明人： Hideo Hirohara , Shigeyasu Nabeshima , Tsuneyuki Nagase
IPC分类号： C12P41/00 , C12P13/08 , C12R1/465 , C07B19/02
CPC分类号： C12P13/08
摘要： A method for optical resolution of DL-lysine alkyl ester or acid-addition salts thereof by contacting an aqueous solution of the ester or salts thereof with a non-specific protease obtained by culturing bacteria belonging to the genus Streptomyces, e.g. St. griseus, at weakly acidic pH and a temperature not exceeding 60.degree. C until 60 - 90% by weight of L-lysine ester or acid-addition salts thereof takes part in a reaction, and recovering L-lysine or acid-addition salts thereof thus produced by a conventional manner. The protease may be immobilized on an insoluble carrier in advance.
摘要翻译：通过使其酯或盐的水溶液与通过培养属于链霉菌属（Streptomyces）属的细菌获得的非特异性蛋白酶接触来使DL-赖氨酸烷基酯或其酸加成盐进行光学拆分的方法。圣灰泥，在弱酸性pH和不超过60℃的温度下，直到60-90重量％的L-赖氨酸酯或其酸加成盐参与反应，并回收L-赖氨酸或酸加成盐因此通过常规方式产生。蛋白酶可以预先固定在不溶性载体上。

5. 发明授权

US5173423A Process for breeding a glabrous variety of rice crop and a glabrous plant 失效
标题翻译：育种无花果种的水稻作物和无毛植物的过程
公开(公告)号：US5173423A
公开(公告)日：1992-12-22
申请号：US479670
申请日：1990-02-12
申请人： Akira Nishikawa , Toshiya Yamamoto , Seiichi Tuji , Hirochika Sakano , Hideo Hirohara
发明人： Akira Nishikawa , Toshiya Yamamoto , Seiichi Tuji , Hirochika Sakano , Hideo Hirohara
IPC分类号： A01H1/02 , A01H4/00 , A01H5/10 , C12N5/00 , C12N5/04
CPC分类号： C12N5/04 , A01H1/02 , A01H4/00 , A01H5/10 , C12N5/0025
摘要： Disclosed herein is a process for breeding a glabrous variety of rice crop, this process comprising the steps of culturing tissues from a target rice plant, culturing the calli formed to a regeneration medium, growing the regenerates and selecting glabrous plants therefrom them, a new and distinct variety of a glabrous variety of a rice plant obtained by this process, and a process for the production of a glabrous plant which comprises further reproducing the plants. This glabrous variety is useful for rice cultivation.
摘要翻译：本发明是一种育种无花果种稻作物的方法，该方法包括以下步骤：从目标水稻植物培养组织，培养形成的愈伤组织至再生培养基，培养再生并从中选择无毛植物，通过该方法获得的具有不同种类的水稻植物的不同种类，以及用于生产无毛植物的方法，其包括进一步再现植物。这种无毛的品种对水稻种植很有用。

6. 发明授权

US4255521A Method for isolation of glucose isomerase 失效
标题翻译：葡萄糖异构酶分离方法
公开(公告)号：US4255521A
公开(公告)日：1981-03-10
申请号：US40872
申请日：1979-05-21
申请人： Hideo Hirohara , Shigeyasu Nabeshima , Satoshi Mitsuda , Tsuneyuki Nagase
发明人： Hideo Hirohara , Shigeyasu Nabeshima , Satoshi Mitsuda , Tsuneyuki Nagase
IPC分类号： C12N1/06 , C12N9/92
CPC分类号： C12N9/92 , C12N1/066 , Y10S435/803 , Y10S435/886 , Y10S435/887
摘要： In a process for extracting glucose isomerase from microorganism cells containing glucose isomerase by the so called high pressure release and impact method in which the suspension of the microorganism cells is homogenized for the extraction by releasing the pressure applied on said suspension instantaneously and giving the suspension an impact at very high velocity, the concentration of the suspension containing microorganism cells and the pressure applied on the said suspension are selected within specific ranges, namely from 0.5 to 10 wt. % (on a dry substance base) and from 400 to 700 Kg/cm.sup.2, respectively.
摘要翻译：在通过所谓的高压释放和冲击法从含有葡萄糖异构酶的微生物细胞中提取葡萄糖异构酶的方法中，其中微生物细胞的悬浮液通过释放立即施加在所述悬浮液上的压力并将悬浮液以非常高的速度冲击，含有微生物细胞的悬浮液的浓度和施加在所述悬浮液上的压力在特定范围内选择，即0.5至10wt。％（在干物质基础上）和400至700Kg / cm 2。

7. 发明授权

US4985365A Process for producing optically active benzyl alcohol compound 失效
标题翻译：光学活性苄醇化合物的制造方法
公开(公告)号：US4985365A
公开(公告)日：1991-01-15
申请号：US201927
申请日：1988-06-03
申请人： Satoshi Mitsuda , Noritada Matsuo , Hideo Hirohara
发明人： Satoshi Mitsuda , Noritada Matsuo , Hideo Hirohara
IPC分类号： A01N37/38 , C12P7/02 , C12P13/00 , C12P41/00
CPC分类号： C12P13/004 , A01N37/38 , C07C255/00 , C12P41/004 , Y10S435/822
摘要： A process for preparing resolved (S)-(-)-.alpha.-cyano-3-phenoxybenzyl alcohol or an (S)-isomer-rich optically active .alpha.-cyano-3-phenoxybenzyl alcohol compound.
摘要翻译：一种制备分解的（S） - （ - ） - α-氰基-3-苯氧基苄醇或（S） - 异构体的光学活性α-氰基-3-苯氧基苄醇化合物的方法。

8. 发明授权

US4247642A Enzyme immobilization with pullulan gel 失效
标题翻译：酶固定与支链淀粉凝胶
公开(公告)号：US4247642A
公开(公告)日：1981-01-27
申请号：US878572
申请日：1978-02-16
申请人： Hideo Hirohara , Shigeyasu Nabeshima , Masanori Fujimoto , Tsuneyuki Nagase
发明人： Hideo Hirohara , Shigeyasu Nabeshima , Masanori Fujimoto , Tsuneyuki Nagase
IPC分类号： C08B37/00 , C12N11/10
CPC分类号： C08B37/0018 , C12N11/10
摘要： Enzymes are immobilized by a process wherein the carrier used for immobilizing is a hydrophilic pullulan gel having a three-dimensionally reticulated structure obtained by the reaction between pullulan and a bifunctional compound capable of forming an ether linkage with the hydroxyl group contained in the glucose unit of pullulan, or an ionic pullulan gel obtained by the reaction between said hydrophilic pullulan gel and a compound having at one end an ionizable group and at the other end a functional group capable of forming an ether linkage with the hydroxyl group in the pullulan gel in the presence of an alkaline coumpound. Immobilized enzymes prepared according to this process have a high activity and good retention of the activity.
摘要翻译：通过一种方法将酶固定化，其中用于固定的载体是具有三维网状结构的亲水支链淀粉凝胶，其通过支链淀粉与能够与葡萄糖单元中所含的羟基形成醚键的双官能化合物反应而获得支链淀粉或通过所述亲水支链淀粉凝胶和一端具有可离子化基团的反应获得的离子支链淀粉凝胶，另一端是能够与支链淀粉凝胶中的羟基形成醚键的官能团碱性化合物的存在。根据该方法制备的固定化酶具有高活性和良好的活性保留性。

9. 发明授权

US4170696A Enzyme-immobilization carrier and preparation thereof 失效
标题翻译：酶固定载体及其制备
公开(公告)号：US4170696A
公开(公告)日：1979-10-09
申请号：US875120
申请日：1978-02-03
申请人： Hideo Hirohara , Shigeyasu Nabeshima , Tsuneyuki Nagase
发明人： Hideo Hirohara , Shigeyasu Nabeshima , Tsuneyuki Nagase
IPC分类号： C08F8/00 , C08F8/32 , C08G85/00 , C12N11/02 , C12N11/08 , C08G2/00
CPC分类号： C12N11/08 , C08G85/00
摘要： A diethylaminoethyl derivative or its salt is reacted with a synthetic resin of a macroporous type having functional groups reactive with said diethylaminoethyl derivative or its salt, having a specific area of at least 1 m.sup.2 /g-dry resin and containing macropores of which the total volume of those with pore sizes from 100 A to 2,000 A is at least 0.1 cc/g-dry resin, in the presence of an alkaline compound to give an enzyme-immobilization carrier which can provide an immobilized enzyme high in activity with good activity retentivity and also large in amount of the immobilized enzyme per unit weight of the carrier.
摘要翻译：二乙基氨基乙基衍生物或其盐与具有与所述二乙基氨基乙基衍生物或其盐反应的官能团的大孔类型的合成树脂反应，比表面积至少为1m 2 / g-干树脂，并含有其总体积在碱性化合物的存在下，孔径为100A至2,000A的那些是至少0.1cc / g-干树脂，得到能够提供具有良好活性保持性的活性高的固定化酶的酶固定载体，每单位重量载体的固定化酶量也很大。

10. 发明授权

US4146432A Immobilized proteases 失效
标题翻译：固定蛋白酶
公开(公告)号：US4146432A
公开(公告)日：1979-03-27
申请号：US801246
申请日：1977-05-27
申请人： Hideo Hirohara , Shigeyasu Nabeshima , Tsuneyuki Nagase
发明人： Hideo Hirohara , Shigeyasu Nabeshima , Tsuneyuki Nagase
IPC分类号： C12N9/52 , C12N11/08 , C12N11/10 , C07G7/02
CPC分类号： C12N9/52 , C12N11/08 , C12N11/10
摘要： Non-specific proteases produced by bacteria belonging to the genus Streptomyces are immobilized by adsorption on a specific class of anion exchangers to produce water-insoluble enzymatically active immobilized proteases.
摘要翻译：通过吸附在特定类型的阴离子交换剂上固定属于链霉菌属的细菌产生的非特异性蛋白酶，以产生不溶于水的酶活性固定蛋白酶。

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