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1. 发明申请

US20110159545A1 Fungamyl-like Alpha-Amylase Variants 审中-公开
标题翻译：戊酰样α-淀粉酶变体
公开(公告)号：US20110159545A1
公开(公告)日：2011-06-30
申请号：US13037854
申请日：2011-03-01
申请人： Henrik Bisgård-Frantzen , Allan Svendsen , Sven Pedersen
发明人： Henrik Bisgård-Frantzen , Allan Svendsen , Sven Pedersen
IPC分类号： C12P19/00 , C12N9/30 , C07H21/04 , A23L1/09 , C12C11/00 , A21D8/06 , A21D8/04
CPC分类号： C12Y302/01001 , A21D8/042 , C12C5/004 , C12C11/00 , C12N9/2417 , C12N9/242 , C12P7/06 , C12P19/02 , C12P19/14 , C13K1/06 , C13K7/00 , Y02E50/17
摘要： The invention relates to a variant of a parent Fungamyl-like fungal alpha-amylase, which exhibits improved thermal stability at acidic pH suitable for, e.g., starch processes.
摘要翻译：本发明涉及亲本的Fungamyl样真菌α-淀粉酶的变体，其在适于例如淀粉方法的酸性pH下表现出改进的热稳定性。

2. 发明授权

US07341858B2 Fungamyl-like alpha-amylase variants 有权
标题翻译：戊酰样α-淀粉酶变体
公开(公告)号：US07341858B2
公开(公告)日：2008-03-11
申请号：US11633385
申请日：2006-12-04
申请人： Henrik Bisgård-Frantzen , Allan Svendsen , Sven Pedersen
发明人： Henrik Bisgård-Frantzen , Allan Svendsen , Sven Pedersen
IPC分类号： C12N9/30 , C12N15/00 , C07K1/00 , C12P19/14 , C12P7/02
CPC分类号： C12Y302/01001 , A21D8/042 , C12C5/004 , C12C11/00 , C12N9/2417 , C12N9/242 , C12P7/06 , C12P19/02 , C12P19/14 , C13K1/06 , C13K7/00 , Y02E50/17
摘要： The invention relates to a variant of a parent Fungamyl-like fungal alpha-amylase, which exhibits improved thermal stability at acidic pH suitable for, e.g., starch processes.
摘要翻译：本发明涉及亲本的Fungamyl样真菌α-淀粉酶的变体，其在适于例如淀粉方法的酸性pH下表现出改进的热稳定性。

3. 发明授权

US07005288B1 Fungamyl-like alpha-amylase variants 有权
标题翻译：戊酰样α-淀粉酶变体
公开(公告)号：US07005288B1
公开(公告)日：2006-02-28
申请号：US09710339
申请日：2000-11-09
申请人： Henrik Bisgård-Frantzen , Allan Svendsen , Sven Pedersen
发明人： Henrik Bisgård-Frantzen , Allan Svendsen , Sven Pedersen
IPC分类号： C12N9/30 , C12N15/00 , C12N1/20 , C12P19/14 , C12P7/02
CPC分类号： C12Y302/01001 , A21D8/042 , C12C5/004 , C12C11/00 , C12N9/2417 , C12N9/242 , C12P7/06 , C12P19/02 , C12P19/14 , C13K1/06 , C13K7/00 , Y02E50/17
摘要： The invention relates to a variant of a parent Fungamyl-like fungal alpha-amylase, which exhibits improved thermal stability at acidic pH suitable for, e.g., starch processes.
摘要翻译：本发明涉及亲本的Fungamyl样真菌α-淀粉酶的变体，其在适于例如淀粉方法的酸性pH下表现出改进的热稳定性。

4. 发明授权

US06623948B1 Nucleic acid sequences encoding alkaline alpha-amylases 有权
标题翻译：编码碱性α-淀粉酶的核酸序列
公开(公告)号：US06623948B1
公开(公告)日：2003-09-23
申请号：US09540715
申请日：2000-03-31
申请人： Helle Outtrup , Lisbeth Hedegaard Hoeck , Bjarne Ronfeldt Nielsen , Torben Vedel Borchert , Vibeke Skovgaard Nielsen , Henrik Bisgård-Frantzen , Allan Svendsen , Carsten Andersen
发明人： Helle Outtrup , Lisbeth Hedegaard Hoeck , Bjarne Ronfeldt Nielsen , Torben Vedel Borchert , Vibeke Skovgaard Nielsen , Henrik Bisgård-Frantzen , Allan Svendsen , Carsten Andersen
IPC分类号： C12N928
CPC分类号： C12N9/2417 , C11D3/386
摘要： The present invention relates to isolated nucleic acid sequences encoding polypeptides having alpha-amylase activity [E.C. 3.2.1.1], which may be derived from Bacillus. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the nucleic acid sequences as well as methods for producing and using the polypeptides.
摘要翻译：本发明涉及编码具有α-淀粉酶活性的多肽的分离的核酸序列[E.C. 3.2.1.1]，其可以衍生自芽孢杆菌属。本发明还涉及包含核酸序列的核酸构建体，载体和宿主细胞以及产生和使用多肽的方法。

5. 发明授权

US06436888B1 &agr;-amylase mutants 有权
标题翻译： α-淀粉酶突变体
公开(公告)号：US06436888B1
公开(公告)日：2002-08-20
申请号：US09672459
申请日：2000-09-28
申请人： Allan Svendsen , Torben Vedel Borchert , Henrik Bisgård-Frantzen
发明人： Allan Svendsen , Torben Vedel Borchert , Henrik Bisgård-Frantzen
IPC分类号： C12N928
CPC分类号： C12N9/2417 , C11D3/386 , C12P19/02 , C12P19/14
摘要： The invention relates to a variant of a parent Termamyl-like a-amylase, which variant has a-amylase activity and exhibits an alteration in at least one of the following properties relative to parent a-amylase: substrate specificity, substrate binding, substrate cleavage pattern, thermal stability, pH/activity profile, pH/stability profile, stability towards oxidation, Ca2+ dependency and specific activity.
摘要翻译：本发明涉及母体类戊酰样α-淀粉酶的变体，该变体具有α-淀粉酶活性并且相对于亲本α-淀粉酶在下列特性中表现出至少一个变化：底物特异性，底物结合，底物切割图案，热稳定性，pH /活性曲线，pH /稳定性分布，对氧化的稳定性，Ca 2+依赖性和比活性。

6. 发明授权

US07816113B2 Starch debranching enzymes 失效
标题翻译：淀粉脱支酶
公开(公告)号：US07816113B2
公开(公告)日：2010-10-19
申请号：US12115123
申请日：2008-05-05
申请人： Henrik Bisgård-Frantzen , Allan Svendsen
发明人： Henrik Bisgård-Frantzen , Allan Svendsen
IPC分类号： C12N9/44 , C12N9/24 , C12N9/00 , C12P19/16 , C12P19/20 , C12P21/06 , C07H21/04
CPC分类号： C12N9/2457 , C12N9/2451 , C12N9/246 , C12P19/16 , C12Y302/01041 , C12Y302/01068
摘要： The invention relates to a genetically engineered variant of a parent starch debranching enzyme, i.e. a pullulanase or an isoamylase, the enzyme variant having an improved thermostability at a pH in the range of 4-6 compared to the parent enzyme and/or an increased activity towards amylopectin and/or glycogen compared to the parent enzyme, to methods for producing such starch debranching enzyme variants with improved thermostability and/or altered substrate specificity, and to a method for converting starch to one or more sugars using at least one such enzyme variant.
摘要翻译：本发明涉及母体淀粉脱支酶（即支链淀粉酶或异淀粉酶）的遗传改造变体，所述酶变体在与亲本酶相比在4-6的范围内具有改善的热稳定性和/或增加的活性对于支链淀粉和/或糖原，与制备具有改进的热稳定性和/或改变的底物特异性的淀粉脱支酶变体的方法以及使用至少一种这样的酶变体将淀粉转化为一种或多种糖的方法。

7. 发明授权

US06448049B1 Starch conversion process 有权
标题翻译：淀粉转化过程
公开(公告)号：US06448049B1
公开(公告)日：2002-09-10
申请号：US09544123
申请日：2000-04-06
申请人： Noriko Tsutsumi , Henrik Bisgård-Frantzen , Allan Svendsen
发明人： Noriko Tsutsumi , Henrik Bisgård-Frantzen , Allan Svendsen
IPC分类号： C12N944
CPC分类号： C12N9/246 , C12N9/2451 , C12P19/16 , C12Y302/01001 , C12Y302/01068
摘要： The present invention relates to a starch conversion process of the type which includes a debranching step wherein an isoamylase being active at the process conditions prevailing is used for debranching the starch and to the use of thermostable isoamylases for starch conversion. The invention further relates to an isolated isoamylase obtained from a strain of the genus Rhodothermus and to cloned DNA sequences encoding isoamylases derived from a strain of Rhodothermus or Sulfolobus, to expression vectors comprising said DNA sequence, host cells comprising such expression vectors, and finally to methods for producing said isoamylases.
摘要翻译：本发明涉及一种淀粉转化方法，其包括脱支步骤，其中使用主要工艺条件的活性的异淀粉酶用于脱淀淀粉和使用热稳定的异戊酰化酶进行淀粉转化。本发明还涉及从Rhodothermus菌株获得的分离的异淀粉酶，以及克隆编码来自Rhodothermus或Sulfolobus菌株的异戊酰化酶的DNA序列，包含所述DNA序列的表达载体，包含该表达载体的宿主细胞，最后到生产所述异戊酰化酶的方法。

8. 发明申请

US20120122754A1 Alpha-Amylase Variants 有权
标题翻译： α-淀粉酶变体
公开(公告)号：US20120122754A1
公开(公告)日：2012-05-17
申请号：US13355047
申请日：2012-01-20
申请人： Carsten Andersen , Christel Thea Jørgensen , Henrik Bisgård-Frantzen , Allan Svendsen , Søren Kjærulff
发明人： Carsten Andersen , Christel Thea Jørgensen , Henrik Bisgård-Frantzen , Allan Svendsen , Søren Kjærulff
IPC分类号： C11D3/386 , C12N9/28
CPC分类号： C12N9/2417 , C07K2319/00 , C12P19/04 , C12P19/14 , C12Y302/01001 , Y02E50/17 , Y02P20/52
摘要： The invention relates to a variant of a parent Termamyl-like alpha-amylase, which variant exhibits altered properties, in particular reduced capability of cleaving a substrate close to the branching point, and improved substrate specificity and/or improved specific activity relative to the parent alpha-amylase.
摘要翻译：本发明涉及母体类戊酰样α-淀粉酶的变体，该变体表现出改变的性质，特别是降低了切割接近分支点的底物的能力，以及相对于母体改善的底物特异性和/或改善的比活性 α-淀粉酶。

9. 发明授权

US07115409B1 α-amylase mutants 失效
标题翻译： α-淀粉酶突变体
公开(公告)号：US07115409B1
公开(公告)日：2006-10-03
申请号：US09327563
申请日：1999-06-08
申请人： Allan Svendsen , Henrik Bisgård-Frantzen , Torben Vedel Borchert
发明人： Allan Svendsen , Henrik Bisgård-Frantzen , Torben Vedel Borchert
IPC分类号： C12N9/26 , C12N9/28 , C07H21/04
CPC分类号： C12N9/2417 , C11D3/386
摘要： The present invention relates to a method of constructing a variant of a parent Termamyl-like α-amylase, which variant has α-amylase activity and at least one altered property as compared to the parent α-amylase, comprises i) analysing the structure of the parent Termamyl-like α-amylase to identify at least one amino acid residue or at least one structural part of the Termamyl-like α-amylase structure, which amino acid residue or structural part is believed to be of relevance for altering the property of the parent Termamyl-like α-amylase (as evaluated on the basis of structural or functional considerations), ii) constructing a Termamyl-like α-amylase variant, which as compared to the parent Termamyl-like α-amylase, has been modified in the amino acid residue or structural part identified in i) so as to alter the property, and, optionally, iii) testing the resulting Termamyl-like α-amylase variant with respect to the property in question.
摘要翻译：本发明涉及一种构建母体类戊酰样α-淀粉酶的变体的方法，该变体具有与母体α-淀粉酶相比具有α-淀粉酶活性和至少一种改变的性质，包括：i）分析鉴定至少一个氨基酸残基或类甲酰样α-淀粉酶结构的至少一个结构部分，所述氨基酸残基或结构部分被认为与改变其性质有关的相关性母体类戊酰样α-淀粉酶（根据结构或功能考虑进行评估），ii）构建与母体类戊酰样α-淀粉酶相比的类戊酰样α-淀粉酶变体，已经在在i）中鉴定的氨基酸残基或结构部分，以改变其性质，以及任选地，iii）相对于所讨论的性质测试所得的类戊酰样α-淀粉酶变体。

10. 发明授权

US06440716B1 &agr;-amylase mutants 失效
标题翻译： α-淀粉酶突变体
公开(公告)号：US06440716B1
公开(公告)日：2002-08-27
申请号：US09636252
申请日：2000-08-10
申请人： Allan Svendsen , Henrik Bisgård-Frantzen , Torben Vedel Borchert
发明人： Allan Svendsen , Henrik Bisgård-Frantzen , Torben Vedel Borchert
IPC分类号： C12N928
CPC分类号： C11D3/386 , C12N9/2417
摘要： The present invention relates to a method of constructing a variant of a parent Termamyl-like &agr;-amylase, which variant has &agr;-amylase activity and at least one altered property as compared to the parent &agr;-amylase, comprises i) analysing the structure of the parent Termamyl-like &agr;-amylase to identify at least one amino acid residue or at least one structural part of the Termamyl-like &agr;-amylase structure, which amino acid residue or structural part is believed to be of relevance for altering the property of the parent Termamyl-like &agr;-amylase (as evaluated on the basis of structural or functional considerations), ii) constructing a Termamyl-like &agr;-amylase variant, which as compared to the parent Termamyl-like &agr;-amylase, has been modified in the amino acid residue or structural part identified in i) so as to alter the property, and, optionally, iii) testing the resulting Termamyl-like &agr;-amylase variant with respect to the property in question.
摘要翻译：本发明涉及一种构建母体类戊酰样α-淀粉酶的变体的方法，该变体与母体α-淀粉酶相比具有α-淀粉酶活性和至少一种改变的性质，包括：i）分析母体类戊酰样α-淀粉酶，以鉴定至少一个氨基酸残基或至少一个类甲酰样α-淀粉酶结构的结构部分，该氨基酸残基或结构部分被认为与改变亲本类戊酰样α-淀粉酶（根据结构或功能考虑进行评估），ii）构建与母体类戊酰样α-淀粉酶相比较的类戊酰样α-淀粉酶变体，其在在i）中鉴定的氨基酸残基或结构部分，以便改变其性质，以及任选地，iii）相对于所讨论的性质测试所得到的类戊酰样α-淀粉酶变体。

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