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1. 发明申请

US20120042414A1 CHIMERIC PROMOTERS AND METHODS OF USE 有权
标题翻译： CHIMERIC PROMOTERS和使用方法
公开(公告)号：US20120042414A1
公开(公告)日：2012-02-16
申请号：US13209017
申请日：2011-08-12
申请人： Henrik Albert , Linda A. Castle , Jian Lu , Yumin Tao
发明人： Henrik Albert , Linda A. Castle , Jian Lu , Yumin Tao
IPC分类号： A01H1/00 , A01H5/00 , C07H21/04 , C12N15/82 , C12N15/63
CPC分类号： C12N15/8221 , C12N9/0069 , C12N15/82 , C12N15/8274 , C12Y113/11027
摘要： Compositions and methods comprising promoters from the 4-hydroxyphenylpyruvate dioxygenase (HPPD) gene and active variants and fragments thereof, as well as chimeric promoters employing regulatory regions of the HPPD promoters are provided. Further provided are expression cassettes and plants comprising the various promoters disclosed herein operably linked to a polynucleotide of interest. Methods employing the various promoters described herein to modulate the expression of polynucleotides of interest are further provided.
摘要翻译：提供了包含来自4-羟基苯基丙酮酸双加氧酶（HPPD）基因及其活性变体和片段的启动子的组合物和方法，以及采用HPPD启动子调节区的嵌合启动子。还提供了包含本文公开的各种启动子的表达盒和植物，其与感兴趣的多核苷酸可操作地连接。进一步提供了使用本文所述的各种启动子来调节感兴趣的多核苷酸的表达的方法。

2. 发明申请

US20120042412A1 Methods And Compositions For Targeting Sequences Of Interest To The Chloroplast 有权
标题翻译：用于靶向叶绿体感兴趣的方法和组合物
公开(公告)号：US20120042412A1
公开(公告)日：2012-02-16
申请号：US13208960
申请日：2011-08-12
申请人： Henrik Albert , Linda A. Castle , Matthew Heckert , Jian Lu , Daniel L. Siehl , Yumin Tao
发明人： Henrik Albert , Linda A. Castle , Matthew Heckert , Jian Lu , Daniel L. Siehl , Yumin Tao
IPC分类号： A01H1/06 , C12N15/82 , C12N5/10 , C12N9/96 , A01H5/10 , C07K19/00 , C12N9/02 , C12N15/62 , A01H5/00
CPC分类号： C12N15/8221 , C12N9/0069 , C12N15/82 , C12N15/8274 , C12Y113/11027
摘要： Chimeric polynucleotides comprising a nucleotide sequence encoding a chloroplast transit peptide operably linked to a heterologous polynucleotide of interest are provided, wherein the chloroplast transit peptide comprises an amino acid sequence having the chloroplast transit peptide sequence as set forth in SEQ ID NO:1 or a biologically active variant or fragment thereof or wherein the chloroplast transit peptide comprises the sequence set forth in SEQ ID NO: 58 or an active variant or fragment thereof. Chimeric polypeptides encoding the same, as well as, cells, plant cells, plants and seeds are further provided which comprise the chimeric polynucleotides. Compositions further include HPPD polypeptides and polynucleotides encoding the same as set forth in SEQ ID NOS: 57 and 60 or active variants and fragments thereof. Such sequences comprise the chloroplast transit peptide as set forth in SEQ ID NO: 58 or an active variants or fragments thereof. Cells, plant cells, plants and seeds are further provided which comprise such sequences. Methods of use of the various sequences are also provided.
摘要翻译：提供了包含编码与目标异源多核苷酸有效连接的叶绿体转运肽的核苷酸序列的嵌合多核苷酸，其中叶绿体转运肽包含具有如SEQ ID NO：1所示的叶绿体转运肽序列的氨基酸序列或生物学上活性变体或其片段，或其中叶绿体转运肽包含SEQ ID NO：58所示的序列或其活性变体或片段。还提供了编码其的嵌合多肽，以及细胞，植物细胞，植物和种子，其包含嵌合多核苷酸。组合物还包括编码与SEQ ID NO：57和60所示相同的HPPD多肽和多核苷酸或其活性变体和片段。这样的序列包含如SEQ ID NO：58所示的叶绿体转运肽或其活性变体或片段。进一步提供包含这些序列的细胞，植物细胞，植物和种子。还提供了使用各种序列的方法。

3. 发明授权

US09187762B2 Compositions and methods comprising sequences having hydroxyphenylpyruvate dioxygenase (HPPD) activity 有权
标题翻译：包含具有羟基苯基丙酮酸双加氧酶（HPPD）活性的序列的组合物和方法
公开(公告)号：US09187762B2
公开(公告)日：2015-11-17
申请号：US13208966
申请日：2011-08-12
申请人： Henrik Albert , Ericka R. Bermudez , Linda A. Castle , Yuxia Dong , Matthew J. Heckert , Jingtong Hou , Zhenglin Hou , Jian Lu , Daniel L. Siehl , Yumin Tao
发明人： Henrik Albert , Ericka R. Bermudez , Linda A. Castle , Yuxia Dong , Matthew J. Heckert , Jingtong Hou , Zhenglin Hou , Jian Lu , Daniel L. Siehl , Yumin Tao
IPC分类号： C12N15/00 , C12N5/10 , A01H5/00 , C12N15/82 , C12N9/02
CPC分类号： C12N15/8221 , C12N9/0069 , C12N15/82 , C12N15/8274 , C12Y113/11027
摘要： Compositions and methods comprising polynucleotides and polypeptides having 4-hydroxyphenylpyruvate dioxygenase (HPPD) activity and having insensitivity to an HPPD inhibitor are provided. Further provided are nucleic acid constructs, plants, plant cells, explants, seeds and grain having the HPPD sequences. Various methods of employing the HPPD sequences are provided. Such methods include, for example, methods for producing an HPPD inhibitor tolerant plant, plant cell, explant or seed and methods of controlling weeds in a field containing a crop employing the plants and/or seeds disclosed herein. Methods are also provided to identify additional HPPD variants. Further provided are various methods and compositions that allow the various HPPD polypeptides and variant and fragments thereof to be expressed in a chloroplast or transported to a chloroplast.
摘要翻译：提供了包含具有4-羟基苯基丙酮酸双加氧酶（HPPD）活性并且对HPPD抑制剂不敏感的多核苷酸和多肽的组合物和方法。还提供了具有HPPD序列的核酸构建体，植物，植物细胞，外植体，种子和谷粒。提供了使用HPPD序列的各种方法。这样的方法包括例如用于产生HPPD抑制剂耐受植物，植物细胞，外植体或种子的方法以及在含有本文公开的植物和/或种子的作物的田地中控制杂草的方法。还提供了用于鉴定另外的HPPD变体的方法。进一步提供了允许各种HPPD多肽及其变体和片段在叶绿体中表达或转运至叶绿体的各种方法和组合物。

4. 发明授权

US09139842B2 Methods and compositions for targeting sequences of interest to the chloroplast 有权
标题翻译：靶向叶绿体感兴趣序列的方法和组合物
公开(公告)号：US09139842B2
公开(公告)日：2015-09-22
申请号：US13208960
申请日：2011-08-12
申请人： Henrik Albert , Linda A. Castle , Matthew Heckert , Jian Lu , Daniel L. Siehl , Yumin Tao
发明人： Henrik Albert , Linda A. Castle , Matthew Heckert , Jian Lu , Daniel L. Siehl , Yumin Tao
IPC分类号： C12N15/82 , C12N9/02
CPC分类号： C12N15/8221 , C12N9/0069 , C12N15/82 , C12N15/8274 , C12Y113/11027
摘要： Chimeric polynucleotides comprising a nucleotide sequence encoding a chloroplast transit peptide operably linked to a heterologous polynucleotide of interest are provided, wherein the chloroplast transit peptide comprises an amino acid sequence having the chloroplast transit peptide sequence as set forth in SEQ ID NO:1 or a biologically active variant or fragment thereof or wherein the chloroplast transit peptide comprises the sequence set forth in SEQ ID NO: 58 or an active variant or fragment thereof. Chimeric polypeptides encoding the same, as well as, cells, plant cells, plants and seeds are further provided which comprise the chimeric polynucleotides. Compositions further include HPPD polypeptides and polynucleotides encoding the same as set forth in SEQ ID NOS: 57 and 60 or active variants and fragments thereof. Such sequences comprise the chloroplast transit peptide as set forth in SEQ ID NO: 58 or an active variants or fragments thereof. Cells, plant cells, plants and seeds are further provided which comprise such sequences. Methods of use of the various sequences are also provided.
摘要翻译：提供了包含编码与目标异源多核苷酸有效连接的叶绿体转运肽的核苷酸序列的嵌合多核苷酸，其中叶绿体转运肽包含具有如SEQ ID NO：1所示的叶绿体转运肽序列的氨基酸序列或生物学上活性变体或其片段，或其中叶绿体转运肽包含SEQ ID NO：58所示的序列或其活性变体或片段。还提供了编码其的嵌合多肽，以及细胞，植物细胞，植物和种子，其包含嵌合多核苷酸。组合物还包括编码与SEQ ID NO：57和60所示相同的HPPD多肽和多核苷酸或其活性变体和片段。这样的序列包含如SEQ ID NO：58所示的叶绿体转运肽或其活性变体或片段。进一步提供包含这些序列的细胞，植物细胞，植物和种子。还提供了使用各种序列的方法。

5. 发明申请

US20120042413A1 Compositions And Methods Comprising Sequences Having Hydroxyphenylpyruvate Dioxygenase (HPPD) Activity 有权
标题翻译：包含具有羟基苯丙酮酸双加氧酶（HPPD）活性的序列的组合物和方法
公开(公告)号：US20120042413A1
公开(公告)日：2012-02-16
申请号：US13208966
申请日：2011-08-12
申请人： Henrik Albert , Ericka R. Bermudez , Linda A. Castle , Yuxia Dong , Matthew J. Heckert , Jingtong Hou , Zhenglin Hou , Jian Lu , Daniel L. Siehl , Yumin Tao
发明人： Henrik Albert , Ericka R. Bermudez , Linda A. Castle , Yuxia Dong , Matthew J. Heckert , Jingtong Hou , Zhenglin Hou , Jian Lu , Daniel L. Siehl , Yumin Tao
IPC分类号： C12N15/82 , C12N15/63 , C12N5/10 , A01H5/00 , A01H5/10 , C12N9/02 , C12Q1/68 , A01N41/10 , A01N43/80 , A01N43/56 , A01N57/20 , A01N43/90 , A01N43/54 , A01P13/00 , G01N33/573 , C12N15/53
CPC分类号： C12N15/8221 , C12N9/0069 , C12N15/82 , C12N15/8274 , C12Y113/11027
摘要： Compositions and methods comprising polynucleotides and polypeptides having 4-hydroxyphenylpyruvate dioxygenase (HPPD) activity and having insensitivity to an HPPD inhibitor are provided. Further provided are nucleic acid constructs, plants, plant cells, explants, seeds and grain having the HPPD sequences. Various methods of employing the HPPD sequences are provided. Such methods include, for example, methods for producing an HPPD inhibitor tolerant plant, plant cell, explant or seed and methods of controlling weeds in a field containing a crop employing the plants and/or seeds disclosed herein. Methods are also provided to identify additional HPPD variants. Further provided are various methods and compositions that allow the various HPPD polypeptides and variant and fragments thereof to be expressed in a chloroplast or transported to a chloroplast.
摘要翻译：提供了包含具有4-羟基苯基丙酮酸双加氧酶（HPPD）活性并且对HPPD抑制剂不敏感的多核苷酸和多肽的组合物和方法。还提供了具有HPPD序列的核酸构建体，植物，植物细胞，外植体，种子和谷粒。提供了使用HPPD序列的各种方法。这样的方法包括例如用于产生HPPD抑制剂耐受植物，植物细胞，外植体或种子的方法以及在含有本文公开的植物和/或种子的作物的田地中控制杂草的方法。还提供了用于鉴定另外的HPPD变体的方法。还提供允许各种HPPD多肽及其变体和片段在叶绿体中表达或转运至叶绿体的各种方法和组合物。

6. 发明授权

US08993837B2 Chimeric promoters and methods of use 有权
标题翻译：嵌合启动子和使用方法
公开(公告)号：US08993837B2
公开(公告)日：2015-03-31
申请号：US13209017
申请日：2011-08-12
申请人： Henrik Albert , Linda A. Castle , Jian Lu , Yumin Tao
发明人： Henrik Albert , Linda A. Castle , Jian Lu , Yumin Tao
IPC分类号： C12N15/82 , A01H5/00 , C12N15/00 , C12N5/10 , C07H21/04 , C12N9/02
CPC分类号： C12N15/8221 , C12N9/0069 , C12N15/82 , C12N15/8274 , C12Y113/11027
摘要： Compositions and methods comprising promoters from the 4-hydroxyphenylpyruvate dioxygenase (HPPD) gene and active variants and fragments thereof, as well as chimeric promoters employing regulatory regions of the HPPD promoters are provided. Further provided are expression cassettes and plants comprising the various promoters disclosed herein operably linked to a polynucleotide of interest. Methods employing the various promoters described herein to modulate the expression of polynucleotides of interest are further provided.
摘要翻译：提供了包含来自4-羟基苯基丙酮酸双加氧酶（HPPD）基因及其活性变体和片段的启动子的组合物和方法，以及采用HPPD启动子调节区的嵌合启动子。还提供了包含本文公开的各种启动子的表达盒和植物，其与感兴趣的多核苷酸可操作地连接。进一步提供了使用本文所述的各种启动子来调节感兴趣的多核苷酸的表达的方法。

7. 发明申请

US20170336482A1 Systems, Apparatus, and Methods of Nonlinear Terahertz (THz) Magnetic Resonance Measurement 有权
公开(公告)号：US20170336482A1
公开(公告)日：2017-11-23
申请号：US15600029
申请日：2017-05-19
申请人： Harold Young HWANG , Jian Lu , Yaqing Zhang , Benjamin K. Ofori-Okai , Keith A. Nelson , Xian Li
发明人： Harold Young HWANG , Jian Lu , Yaqing Zhang , Benjamin K. Ofori-Okai , Keith A. Nelson , Xian Li
IPC分类号： G01R33/26 , G01R33/60
CPC分类号： G01R33/26 , G01N24/10 , G01R33/323 , G01R33/60
摘要： A nonlinear terahertz (THz) spectroscopy technique uses a sample illuminated by two THz pulses separately. The illumination generates two signals BA and BB, corresponding to the first and second THz pulse, respectively, after interaction with the sample. The interaction includes excitation of at least one ESR transition in the sample. The sample is also illuminated by the two THz pulses together, with an inter-pulse delay τ, generating a third signal BAB. A nonlinear signal BNL is then derived via BNL=BAB−BA−BB. This nonlinear signal BNL can be then processed (e.g., Fourier transform) to study the properties of the sample.

8. 发明授权

US08999371B2 Contrast pattern application for three-dimensional imaging 有权
标题翻译：对比度图案应用三维成像
公开(公告)号：US08999371B2
公开(公告)日：2015-04-07
申请号：US13424200
申请日：2012-03-19
申请人： Jian Lu , David Licata
发明人： Jian Lu , David Licata
IPC分类号： A61B6/14 , A61C9/00 , A61C13/00 , A61K6/00 , A61K6/02 , C09D5/02 , C09D7/12 , C08K3/22 , C09K3/30
CPC分类号： A61K6/002 , A61B2090/395 , A61C9/0053 , A61C9/006 , A61C13/0004 , A61C2201/005 , A61K6/02 , A61K6/10 , A61K47/02 , A61K47/06 , A61K47/10 , A61K47/32 , A61M11/02 , A61M31/005 , B05B11/0037 , C08K2003/2241 , C09D5/004 , C09D5/021 , C09D7/61 , C09D7/63 , C09D139/06 , C09K3/30
摘要： Provided herein are compositions for applying a reference marker pattern or grid to the surface of an object to be imaged three-dimensionally. These compositions can be quick drying for ease of use. Methods of use of the compositions are also provided as well as devices and kits for the application of the compositions.
摘要翻译：本文提供了用于将参考标记图案或网格应用于三维成像的物体的表面的组合物。这些组合物可以快速干燥以方便使用。还提供了组合物的使用方法以及用于组合物的应用的装置和试剂盒。

9. 发明申请

US20120179666A1 METHODS AND SYSTEMS FOR MONITORING AND TRACKING VIDEOS ON THE INTERNET 有权
标题翻译：在互联网上监控和跟踪视频的方法和系统
公开(公告)号：US20120179666A1
公开(公告)日：2012-07-12
申请号：US13360330
申请日：2012-01-27
申请人： Jian Lu , Lun Cui
发明人： Jian Lu , Lun Cui
IPC分类号： G06F17/30
CPC分类号： G06F17/30864 , G06F17/30784 , G06F17/30817 , G06F17/3082 , G06F17/3084
摘要： Method and system for discovering and identifying a video object. The method includes crawling at least one predetermined website, discovering at least one video link at the predetermined website, processing information associated with a first database for storing one or more video links, and determining whether the discovered video link was already discovered before based on at least information associated with the first database. Additionally, the method includes, if the discovered video link is determined not to have been discovered before, updating the first database based on at least information associated with the discovered video link, downloading at least one video object based on at least information associated with the discovered video link, and processing information associated with the downloaded video object.
摘要翻译：用于发现和识别视频对象的方法和系统。该方法包括爬行至少一个预定网站，在预定网站上发现至少一个视频链路，处理与第一数据库相关联的信息以存储一个或多个视频链路，以及基于在之前确定所发现的视频链路是否已经被发现与第一数据库相关联的最少信息。另外，该方法包括：如果确定所发现的视频链路不是以前被发现，则至少基于与所发现的视频链路相关联的信息来更新第一数据库，至少基于至少一个视频对象的信息，发现的视频链接，以及处理与下载的视频对象相关联的信息。

10. 发明申请

US20100226430A1 Robust Single-Pass Variable Bit Rate Encoding 有权
标题翻译：鲁棒单通可变比特率编码
公开(公告)号：US20100226430A1
公开(公告)日：2010-09-09
申请号：US12784203
申请日：2010-05-20
申请人： Eric Hamilton , Jian Lu , Gregory K. Wallace , Peter Chou
发明人： Eric Hamilton , Jian Lu , Gregory K. Wallace , Peter Chou
IPC分类号： H04N7/26
CPC分类号： H04N19/177 , H04N19/115 , H04N19/136 , H04N19/152 , H04N19/172 , H04N19/61
摘要： An encoding manager facilitates robust single-pass variable bit rate video encoding of a video sequence. Before encoding the video sequence, the encoding manager determines the size of a buffer to use for keeping track of over/under used bits generated during encoding. The encoding manager uses the target bit rate for the video sequence and the length of the video sequence to determine the size of the buffer. After allocating bits to a frame of the sequence, the encoding manager determines the quant to use to encode that frame. The determination of a quant to use to encode a frame is informed by the fullness of the buffer. The encoding manager adjusts the quant to use (and thus the aggressiveness of its encoding) in response to the amount of overflow generated thus far by the encoding of the video sequence.
摘要翻译：编码管理器便于视频序列的鲁棒单遍可变比特率视频编码。在对视频序列进行编码之前，编码管理器确定用于跟踪在编码期间生成的过/未使用位的缓冲器的大小。编码管理器使用视频序列的目标比特率和视频序列的长度来确定缓冲器的大小。在将比特分配给序列的帧之后，编码管理器确定用于编码该帧的量。通过缓冲器的丰满度来通知用于编码帧的量化的确定。编码管理器响应于视频序列的编码到目前为止产生的溢出量来调整使用的量化（并因此调整其编码的侵略性）。

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