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1. 发明授权

US4334023A Process for obtaining cholesterol oxidase 失效
标题翻译：获得胆固醇氧化酶的方法
公开(公告)号：US4334023A
公开(公告)日：1982-06-08
申请号：US147460
申请日：1980-05-07
申请人： Helmgard Gauhl , Georg Schawohl , Hans Seidel , Klaus Beaucamp
发明人： Helmgard Gauhl , Georg Schawohl , Hans Seidel , Klaus Beaucamp
IPC分类号： C12N9/08 , C12N9/04 , C12R1/06 , C12R1/465
CPC分类号： C12N9/0006 , Y10S435/83 , Y10S435/886 , Y10S435/898
摘要： The present invention provides a process for obtaining cholesterol oxidase, wherein Streptomyces griseofuscus DSM 40191, Streptomyces hygroscopicus 40771, Streptomyces acidomyceticus DSM 40798 and/or Arthrobacter paraffinens DSM 312 are cultured and the enzyme obtained from the culture supernatant and/or the cells.
摘要翻译：本发明提供一种获得胆固醇氧化酶的方法，其中培养灰霉菌（Streptomyces griseofuscus）DSM 40191，吸水链霉菌40771，链霉菌酸性霉菌DSM 40798和/或节杆菌属链烷烃DSM 312，以及从培养上清液和/或细胞获得的酶。

2. 发明授权

US4343903A Process for obtaining cholesterol esterase from micro-organisms 失效
标题翻译：从微生物获得胆固醇酯酶的方法
公开(公告)号：US4343903A
公开(公告)日：1982-08-10
申请号：US175808
申请日：1980-08-06
申请人： Klaus Beaucamp , Michael Nelboeck , Helmgard Gauhl , Hans Seidel , Wolfgang Gruber , Herwig Brunner
发明人： Klaus Beaucamp , Michael Nelboeck , Helmgard Gauhl , Hans Seidel , Wolfgang Gruber , Herwig Brunner
IPC分类号： C12N1/14 , C12N1/20 , C12N9/18
CPC分类号： C12N9/18
摘要： The present invention provides a process for obtaining cholesterol esterase from micro-organisms, wherein a micro-organism capable of cholesterol esterase formation is cultured in an appropriate nutrient medium in the presence of lecithin as inducer and the enzyme is obtained from the culture medium and/or from the cells.
摘要翻译：本发明提供从微生物获得胆固醇酯酶的方法，其中在卵磷脂作为诱导剂的存在下，在合适的营养培养基中培养能够形成胆固醇酯酶的微生物，并且从培养基和/ 或从细胞。

3. 发明授权

US4360596A Process for the preparation of cholesterol esterase 失效
标题翻译：胆固醇酯酶的制备方法
公开(公告)号：US4360596A
公开(公告)日：1982-11-23
申请号：US175809
申请日：1980-08-06
申请人： Klaus Beaucamp , Michael Nelboeck , Helmgard Gauhl , Hans Seidel , Wolfgang Gruber , Herwig Brunner
发明人： Klaus Beaucamp , Michael Nelboeck , Helmgard Gauhl , Hans Seidel , Wolfgang Gruber , Herwig Brunner
IPC分类号： C12N9/18 , C12Q1/60
CPC分类号： C12N9/18 , C12Q1/60 , Y10S435/874
摘要： The present invention provides a process for obtaining cholesterol esterase from micro-organisms of the genus Pseudomonas, wherein Pseudomonas sp. DSM 1280 or DSM 1281 is cultured in an appropriate culture medium in the presence of an inducer and the enzyme is obtained from the culture medium and/or from the cells.
摘要翻译：本发明提供了从假单胞菌属的微生物获得胆固醇酯酶的方法，其中假单胞菌属 DSM 1280或DSM 1281在诱导剂存在下在合适的培养基中培养，并且从培养基和/或从细胞获得酶。

4. 发明授权

US4237221A Process for obtaining maltose phosphorylase and .beta.-phosphoglucomutase and method of determining .alpha.-amylase using same 失效
标题翻译：获得麦芽糖磷酸化酶和β-磷酸果糖变位酶的方法和使用其确定α-淀粉酶的方法
公开(公告)号：US4237221A
公开(公告)日：1980-12-02
申请号：US953723
申请日：1978-10-20
申请人： Helmgard Gauhl , Hans Seidel
发明人： Helmgard Gauhl , Hans Seidel
IPC分类号： C12N9/10 , C12N9/12 , C12Q1/40 , C12R1/225 , C12R1/24 , C12R1/25 , C12R1/46 , C12Q1/32 , C12Q1/48
CPC分类号： C12N9/1051 , C12N9/12 , C12Q1/40
摘要： The present invention provides a process for obtaining maltose phosphorylase and/or .beta.-phosphoglucomutase from micro-organisms, wherein the starting material used is selected from Lactobacillus brevis DSM 20054, NCIB 8836, 8561 and 8562, Lactobacillus plantarum DSM 20174 and 43, Lactobacillus reuteri DSM 20016, Lactobacillus fermentum DSM 20052, Streptococcus spec. DSM 1118, DSM 119, DSM 1120 and DSM 1121.The present invention also provides a composition and process for determining .alpha.-amylase, wherein maltose phosphorylase and .beta.-phosphoglucomutase obtained by such a process is used, as a crude extract or in an enriched form, optionally with the addition of .alpha.-glucose-1,6-diphosphate and of divalent manganese ions.
摘要翻译：本发明提供从微生物获得麦芽糖磷酸化酶和/或β-磷酸果糖调蛋白酶的方法，其中使用的起始材料选自短乳杆菌DSM 20054，NCIB8836,8561和8562，植物乳杆菌DSM 20174和43，罗伊氏乳杆菌（Lactobacillus reuteri） DSM 20016，Lactobacillus fermentum DSM 20052，Streptococcus spec。 DSM 1118，DSM 119，DSM 1120和DSM 1121.本发明还提供了用于测定α-淀粉酶的组合物和方法，其中使用通过这种方法获得的麦芽糖磷酸化酶和β-磷酸果糖调蛋白酶作为粗提取物或富集形式，任选加入α-葡萄糖-1,6-二磷酸酯和二价锰离子。

5. 发明授权

US5024945A Process for obtaining sarcosine oxidase from microorganisms 失效
标题翻译：从微生物获得肌氨酸氧化酶的方法
公开(公告)号：US5024945A
公开(公告)日：1991-06-18
申请号：US629504
申请日：1984-07-10
申请人： Ulrich Mayr , Helmgard Gauhl , Hans Seidel
发明人： Ulrich Mayr , Helmgard Gauhl , Hans Seidel
IPC分类号： C12N9/02 , C12N9/06 , C12R1/38
CPC分类号： C12N9/0032 , Y10S435/874
摘要： The present invention provides a process for obtaining sarcosine oxidase from micro-organisms by culturing thereof and obtaining the enzyme from the biomass or from the culture broth, wherein a Pseudomonas strain is cultured.
摘要翻译：本发明提供了一种通过培养微生物获得肌氨酸氧化酶并从生物量或培养液中获得酶的方法，其中培养了假单胞菌属菌株。

6. 发明授权

US4615981A Process for obtaining cholesterol esterase from microorganisms 失效
标题翻译：从微生物获得胆固醇酯酶的方法
公开(公告)号：US4615981A
公开(公告)日：1986-10-07
申请号：US663149
申请日：1984-10-22
申请人： Herbert von der Eltz , Helmgard Gauhl , Hans Seidel
发明人： Herbert von der Eltz , Helmgard Gauhl , Hans Seidel
IPC分类号： C12N9/16 , C12N9/18 , C12R1/38
CPC分类号： C12N9/18 , Y10S435/874
摘要： The present invention provides a process for obtaining cholesterol esterase from micro-organisms by culturing a micro-organism capable of forming cholesterol esterase in an appropriate nutrient medium in the presence of an inductor and obtaining the enzyme from the culture liquid and/or from the cells, therein the inductor used is a compound of the general formula: ##STR1## in which R and R.sub.1 are alkyl or alkoxy radicals containing 14 to 18 carbon atoms and R or R.sub.1 can also be a hydrogen atom and R.sub.2 is an alkylamino radical containing 2 to 8 carbon atoms, an alkyl-trimethylammonium radical containing 3 to 8 carbon atoms, an alkylpyridine radical containing up to 4 carbon atoms in the alkyl moiety or a radical of the general formula --CH.sub.2 --(CHOH).sub.n --CH.sub.2 OH, in which n is a whole number of from 1 to 4.
摘要翻译：本发明提供从微生物获得胆固醇酯酶的方法，通过在电感器存在下培养能够在合适营养培养基中形成胆固醇酯酶的微生物，并从培养液和/或细胞中获得酶其中所用的电感是通式为：其中R和R 1为含有14至18个碳原子的烷基或烷氧基的化合物，R或R 1也可以是氢原子，R 2是含有2个烷基氨基的化合物至8个碳原子，含有3至8个碳原子的烷基 - 三甲基铵基团，烷基部分含有至多4个碳原子的烷基吡啶基团或通式-CH 2 - （CHOH）n -CH 2 OH基团，其中n 是从1到4的整数。

7. 发明授权

US4416983A Determination of NAD(P)H or salicylate 失效
标题翻译：测定NAD（P）H或水杨酸
公开(公告)号：US4416983A
公开(公告)日：1983-11-22
申请号：US328312
申请日：1981-12-07
申请人： Albert Roder , Joachim Siedel , Hans Mollering , Hans Seidel , Helmgard Gauhl
发明人： Albert Roder , Joachim Siedel , Hans Mollering , Hans Seidel , Helmgard Gauhl
IPC分类号： G01N33/66 , C12Q1/00 , C12Q1/26 , C12N9/02 , C12Q1/14 , C12Q1/32 , C12Q1/38 , C12Q1/40 , C12Q1/54 , C12Q1/56
CPC分类号： C12Q1/26 , C12Q1/008 , Y10S435/805 , Y10S435/81
摘要： The present invention provides a process for the determination of NAD(P)H or of salicylate, wherein, in a NAD(P)H-dependent reaction, salicylate is decarboxylated by salicylate hydroxylase and a colored material is formed from the decarboxylation product in the presence of tyrosinase by oxidative coupling with an appropriate colored material component, the colored material formed then being determined photometrically.The present invention also provides a reagent for the determination of NADH or NADPH, wherein it contains salicylate, a chromogenic hydrazone or amine, salicylate hydroxylase, tyrosinase and buffer, as well as a reagent for the determination of salicylate, wherein it contains NAD(P)H, a chromogenic hydrazone or amine, salicylate hydroxylase, tyrosinase and buffer.
摘要翻译：本发明提供了测定NAD（P）H或水杨酸盐的方法，其中在NAD（P）H依赖性反应中，水杨酸盐被水杨酸羟化酶脱羧，并且着色材料由通过与合适的着色材料组分的氧化偶联来存在酪氨酸酶，形成的有色材料然后被光度测定。本发明还提供了用于测定NADH或NADPH的试剂，其中含有水杨酸盐，显色腙或胺，水杨酸羟化酶，酪氨酸酶和缓冲液，以及用于测定水杨酸盐的试剂，其中它含有NAD（P ）H，显色腙或胺，水杨酸羟化酶，酪氨酸酶和缓冲液。

8. 发明授权

US4399218A Method and reagent for the determination of glycerin 失效
标题翻译：测定甘油的方法和试剂
公开(公告)号：US4399218A
公开(公告)日：1983-08-16
申请号：US228010
申请日：1981-01-23
申请人： Helmgard Gauhl , Hans Seidel , Gunter Lang , Albert Roder , Joachim Ziegenhorn
发明人： Helmgard Gauhl , Hans Seidel , Gunter Lang , Albert Roder , Joachim Ziegenhorn
IPC分类号： C07D221/04 , C07D231/26 , C07D231/46 , C07D231/48 , C12N9/04 , C12P7/20 , C12Q1/26 , C12Q1/28 , C12Q1/44 , C12Q1/61 , C12R1/66 , G01N33/92 , C12N1/14 , C12Q1/30
CPC分类号： C12N9/0006 , C07D231/26 , C07D231/46 , C12Q1/26 , C12Q1/28 , C12Q2326/00 , C12Q2326/50 , C12Q2326/96
摘要： For the determination of glycerin by oxidation with oxygen in the presence of glycerinoxidase and measurement of the oxygen consumption or of the H.sub.2 O.sub.2 formation, a glycerinoxidase from Aspergillus spec. DSM 1729 is used. A reagent suitable for this method consists of glycerinoxidase from Aspergillus spec. DSM 1729 and a system for the determination of H.sub.2 O.sub.2 and contains additionally, if desired, an agent for the saponification of esterified glycerin.
摘要翻译：为了通过在存在甘油氧化酶的情况下用氧氧化测定甘油，测量氧气消耗量或H2O2的形成，可以从曲霉属规格的甘油氧化酶。 DSM 1729被使用。适合该方法的试剂由曲霉属的甘油氧化酶组成。 DSM 1729和用于测定H 2 O 2的系统，另外如果需要，还含有用于皂化酯化甘油的试剂。

9. 发明授权

US4397952A Process for obtaining glucose dehydrogenase and micro-organism therefor 失效
标题翻译：获得葡萄糖脱氢酶及其微生物的方法
公开(公告)号：US4397952A
公开(公告)日：1983-08-09
申请号：US260480
申请日：1981-05-04
申请人： Detlef von Hoerschelmann , Hans Seidel , Gerhard Berger , Armin Masuth , Klaus Beaucamp , Wolfgang Gruber
发明人： Detlef von Hoerschelmann , Hans Seidel , Gerhard Berger , Armin Masuth , Klaus Beaucamp , Wolfgang Gruber
IPC分类号： C12N1/20 , C12N9/04 , C12Q1/54 , C12Q1/32 , C12R1/085
CPC分类号： C12N9/0006 , C12Q1/54 , C12R1/085 , Y10S435/834
摘要： The present invention provides a process for obtaining glucose dehydrogenase by culturing a micro-organism in an appropriate nutrient medium and obtaining the enzyme from the cell mass or from the culture medium, wherein the micro-organism cultured is Bacillus cereus DSM 1644. The nutrient medium used contains a carbon source which consists predominantly or solely of glycerol.
摘要翻译：本发明提供了通过在合适的营养培养基中培养微生物并从细胞块或培养基获得酶的方法，其中培养的微生物是蜡状芽孢杆菌DSM 1644.营养培养基使用的碳源主要或仅由甘油组成。

10. 发明授权

US4186052A NADH Peroxidase for hydrogen peroxide determination 失效
标题翻译： NADH过氧化物酶测定过氧化氢
公开(公告)号：US4186052A
公开(公告)日：1980-01-29
申请号：US811422
申请日：1977-06-29
申请人： Albert Roder , Hans Mollering , Wolfgang Gruber , Klaus Beaucamp , Hans Seidel , Peter Stahl , Detlef von Hoerschelmann
发明人： Albert Roder , Hans Mollering , Wolfgang Gruber , Klaus Beaucamp , Hans Seidel , Peter Stahl , Detlef von Hoerschelmann
IPC分类号： C12N9/08 , C12Q1/28 , G01N33/50 , C07G7/02 , C12D13/10 , G01N31/14
CPC分类号： C12N9/0065 , C12Q1/28
摘要： A novel peroxidase for reduced nicotinamide-adenine-dinucleotide is provided which peroxidase oxidizes reduced nicotinamide-adenine-dinucleotide with hydrogen peroxide to give nicotinamide-adenine-dinucleotide and water and is characterized by a Michaelis constant K.sub.Mto hydrogen peroxide of 2.8.times.10.sup.-5 M andto reduced nicotinamide-adenine-dinucleotide of 1.7.times.10.sup.-5 M,measured at 25.degree. C. in 0.2M tris buffer of pH 6.0, containing 0.1M potassium acetate. The peroxidase can be prepared by liberating the enzyme from Streptococcus faecalis ATCC 8043 by digestion or by treatment with a surface-active agent and isolating same from the enzyme solution obtained. Hydrogen peroxide is determined in a simple reaction or in a coupled reaction with a specific oxidase by contacting the said peroxidase with the H.sub.2 O.sub.2 producing reaction mixture at a pH of from 6.0 to 9.0 and measuring the change of extinction as a measure of H.sub.2 O.sub.2 and of the concentration of nicotinamide-adenine-dinucleotide initially present in said reaction mixture.
摘要翻译：提供了一种用于还原型烟酰胺 - 腺嘌呤二核苷酸的新型过氧化物酶，其过氧化物酶用过氧化氢氧化还原型烟酰胺 - 腺嘌呤二核苷酸，得到烟酰胺 - 腺嘌呤二核苷酸和水，其特征在于具有2.8x10-5M的过氧化氢的米氏常数KM，降低了1.7×10-5M的烟酰胺 - 腺嘌呤二核苷酸，在25℃下，在含有0.1M醋酸钾的0.2M Tris缓冲液pH6.0中测定。过氧化物酶可以通过消化或通过用表面活性剂处理从粪链球菌ATCC 8043中释放酶制备，并从获得的酶溶液中分离。过氧化氢在简单的反应或与特定氧化酶的偶联反应中通过使所述过氧化物酶与产生H 2 O 2的反应混合物在6.0至9.0的pH下接触并测定作为H 2 O 2和最初存在于所述反应混合物中的烟酰胺 - 腺嘌呤二核苷酸的浓度。

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