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1. 发明申请

US20140087420A1 NOVEL MICROORGANISM RHIZOBIUM SP. KB10 HAVING PROPERTIES OF PROMOTING GROWH OF BOTRYOCOCCUS BRAUNII AND INCREASING FATTY ACID CONTENT 有权
标题翻译：新型微生物RHIZOBIUM SP。 KB10具有促进白僵菌生长和增加脂肪酸含量的特性
公开(公告)号：US20140087420A1
公开(公告)日：2014-03-27
申请号：US13816735
申请日：2013-01-11
申请人： Hee Mock Oh , Chi Yong Ahn , Young Ki Lee , So Ra Ko , Hee Sik Kim
发明人： Hee Mock Oh , Chi Yong Ahn , Young Ki Lee , So Ra Ko , Hee Sik Kim
IPC分类号： C12P39/00 , C10L1/18
CPC分类号： C12P39/00 , C10L1/026 , C10L1/1802 , C10L2200/0476 , C10L2290/26 , C12N1/12 , C12N1/38 , C12P7/64 , C12P7/6409 , C12P7/6427 , C12R1/41 , Y02E50/13
摘要： The present invention relates to Rhizobium sp. KB10 strain having properties of promoting growth of Botryococcus braunii, which is an alga capable of producing biodiesel, and also enhancing production performance of biodiesel. Mores specifically, it relates to novel Rhizobium sp. KB10 strain which has properties of promoting growth of Botryococcus braunii used for biodiesel production and also enhancing content of C18 (i.e., oleate) corresponding to high quality biodiesel component as much as 900%. By using root colonizing bacteria like Rhizobium, it is possible to promote effectively the slow cell growth of Botryococcus braunii and increase as much as possible the oleate amount, which is a high quality biodiesel component. Further, by carrying out mixture culture using such bacteria, problems associated with contamination by other microorganisms during a process of producing biodiesel by culture in an outside environment can be dramatically solved.
摘要翻译：本发明涉及根瘤菌属具有促进生产生物柴油的藻类Botryococcus braunii生长的特性的KB10菌株，以及提高生物柴油的生产性能。具体来说，它涉及新根瘤菌属 KB10菌株具有促进用于生物柴油生产的Botryococcus braunii生长的性质，并且还提高了对应于高质量生物柴油成分高达900％的C18（即油酸酯）含量。通过使用根瘤菌等细菌根瘤菌，可以有效地促进布鲁氏菌（Botryococcus braunii）的缓慢细胞生长，并尽可能地增加油酸盐量，这是高品质的生物柴油成分。此外，通过使用这种细菌进行混合培养，可以显着地解决与在外部环境下通过培养生产生物柴油的过程中由其他微生物污染相关的问题。

2. 发明授权

US09783859B2 Method of screening and quantifying various enzymatic activities using artificial genetic circuits 有权
公开(公告)号：US09783859B2
公开(公告)日：2017-10-10
申请号：US13376783
申请日：2010-06-08
申请人： Seung Goo Lee , Eugene Rha , Su Lim Choi , Jae Jun Song , Jong Hyun Choi , Hee Sik Kim
发明人： Seung Goo Lee , Eugene Rha , Su Lim Choi , Jae Jun Song , Jong Hyun Choi , Hee Sik Kim
IPC分类号： C40B30/00 , C12Q1/68 , C12N15/10
CPC分类号： C12Q1/6897 , C12N15/1086
摘要： A method of detecting and quantifying various enzymatic activities using a constructed artificial genetic circuit GESS (genetic enzyme screening system) for sensing phenolic compounds and a method of screening a trace of activities of target enzymes from a metagenome using the artificial genetic circuit, thereby securing target enzyme genes. When the method for screening and quantifying target enzymatic activity is used, useful genes can be screened from various genetic communities, including environmental or metagenomic libraries, at a single cell level in high throughput (million/day). Further, the sensitivity of the genetic circuit to phenol derivatives and the expression thereof can be controlled, and thus the genetic circuit can rapidly sense and quantify various enzymatic activities. Thus, the method can be advantageously used in the protein engineering technology for enzyme modification. Particularly, it can quantitatively investigate enzymatic activity, and thus can be applied to molecular evolution technology.

3. 发明申请

US20120238470A1 METHOD FOR SCREENING AND QUANTIFYING VARIOUS ENZYME ACTIVITIES USING A GENETIC ENZYME SCREENING SYSTEM 有权
标题翻译：使用遗传酶筛选系统筛选和定量各种酶活性的方法
公开(公告)号：US20120238470A1
公开(公告)日：2012-09-20
申请号：US13376783
申请日：2010-06-08
申请人： Seung Goo Lee , Eugene Rha , Su Lim Choi , Jae Jun Song , Jong Hyun Choi , Hee Sik Kim
发明人： Seung Goo Lee , Eugene Rha , Su Lim Choi , Jae Jun Song , Jong Hyun Choi , Hee Sik Kim
IPC分类号： C40B30/08
CPC分类号： C12Q1/6897 , C12N15/1086
摘要： A method of detecting and quantifying various enzymatic activities using a constructed artificial genetic circuit GESS (genetic enzyme screening system) for sensing phenolic compounds and a method of screening a trace of activities of target enzymes from a metagenome using the artificial genetic circuit, thereby securing target enzyme genes. When the method for screening and quantifying target enzymatic activity is used, useful genes can be screened from various genetic communities, including environmental or metagenomic libraries, at a single cell level in high throughput (million/day). Further, the sensitivity of the genetic circuit to phenol derivatives and the expression thereof can be controlled, and thus the genetic circuit can rapidly sense and quantify various enzymatic activities. Thus, the method can be advantageously used in the protein engineering technology for enzyme modification. Particularly, it can quantitatively investigate enzymatic activity, and thus can be applied to molecular evolution technology.
摘要翻译：使用用于感测酚类化合物的构建的人工遗传回路GESS（遗传酶筛选系统）检测和定量各种酶活性的方法，以及使用人造遗传回路从宏基因组筛选靶酶的活性痕迹的方法，由此确保靶酶基因。当使用筛选和定量靶酶活性的方法时，可以在高通量（百万/天）的单个细胞水平上从各种遗传群落（包括环境或宏基因组文库）筛选有用的基因。此外，可以控制遗传回路对苯酚衍生物的敏感性及其表达，因此遗传回路可以快速感测和定量各种酶活性。因此，该方法可有利地用于酶修饰的蛋白质工程技术中。特别是可以定量研究酶活性，从而可以应用于分子进化技术。

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