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1. 发明授权

US5512466A Control of fruit ripening and senescence in plants 失效
标题翻译：控制植物中的果实成熟和衰老
公开(公告)号：US5512466A
公开(公告)日：1996-04-30
申请号：US809457
申请日：1991-12-17
申请人： Harry J. Klee , Ganesh M. Kishore
发明人： Harry J. Klee , Ganesh M. Kishore
IPC分类号： A01H5/00 , C07H21/04 , C12N9/88 , C12N15/09 , C12N15/60 , C12N15/82 , C12N15/31 , A01H5/08 , C12N15/63
CPC分类号： C12N15/8249 , C12N9/88
摘要： A method for controlling the ripening of fruits and vegetables as well as a method for controlling senescence of plant tissue is described. The method generally embraces the expression of an ACC metabolizing enzyme in the fruit or other desired plant tissue to inhibit the production of ethylene in the fruit or plant tissue. The use of the ACC metabolizing enzyme ACC deaminase is described in detail. The ripening or senescence process in the fruit or plant tissue is inhibited by the expression of the ACC deaminase gene such that the shelf-life and marketability of the fruit or plant is enhanced. The ACC metabolizing enzyme may be used in combination with other methods for reducing ethylene production in transformed plants to further reduce the production of ethylene in the fruit or plant. DNA constructs containing the ACC deaminase gene are also described.
摘要翻译：描述了控制水果和蔬菜成熟的方法以及控制植物组织衰老的方法。该方法通常包含ACC代谢酶在果实或其它所需植物组织中的表达，以抑制水果或植物组织中的乙烯的产生。 ACC代谢酶ACC脱氨酶的使用详细描述。水果或植物组织中的成熟或衰老过程受到ACC脱氨酶基因的表达的抑制，使得水果或植物的保质期和市售性提高。 ACC代谢酶可以与其他减少转化植物中乙烯生产的方法结合使用，以进一步减少水果或植物中乙烯的产生。还描述了含有ACC脱氨酶基因的DNA构建体。

2. 发明授权

US08637283B2 Production of hydrocarbons in microorganisms 有权
标题翻译：在微生物中生产碳氢化合物
公开(公告)号：US08637283B2
公开(公告)日：2014-01-28
申请号：US13005691
申请日：2011-01-13
申请人： Abhay Kumar Singh , Ganesh M. Kishore
发明人： Abhay Kumar Singh , Ganesh M. Kishore
IPC分类号： C12P5/00 , C12Q1/68 , C12N9/02 , C12N9/00 , C12N1/20 , C12N15/00 , C07H21/04
CPC分类号： C12N1/20 , C12N9/0071 , C12N15/52 , C12P5/026 , Y02E50/343
摘要： A recombinant microorganism employing a bacterial pathway to produce a cyclic amino acid (e.g., coronamic acid or norcoronamic acid) and a plant enzyme (ACC oxidase) to oxidize the amino acid and produce an alkene (e.g., 1-butene or propene) is provided herein. Expression of these two biosynthetic modules in various microbial chassis will facilitate alkene production from diverse energy and carbon sources, including sugars, glycerol, CO2, CH4, H2, and sunlight.
摘要翻译：提供了使用细菌途径产生环状氨基酸的重组微生物（例如，冠状氨酸或诺卡诺酸）和植物酶（ACC氧化酶）以氧化氨基酸并产生烯烃（例如1-丁烯或丙烯）这里。这些两个生物合成模块在各种微生物底盘中的表达将促进来自不同能量和碳源（包括糖，甘油，二氧化碳，CH4，H2和阳光）的烯烃生产。

3. 发明申请

US20110177575A1 PRODUCTION OF HYDROCARBONS IN MICROORGANISMS 有权
标题翻译：在微生物中生产碳氢化合物
公开(公告)号：US20110177575A1
公开(公告)日：2011-07-21
申请号：US13005691
申请日：2011-01-13
申请人： Abhay Kumar Singh , Ganesh M. Kishore
发明人： Abhay Kumar Singh , Ganesh M. Kishore
IPC分类号： C12P5/02 , C12N1/00 , C12N1/21 , C12N1/19
CPC分类号： C12N1/20 , C12N9/0071 , C12N15/52 , C12P5/026 , Y02E50/343
摘要： A recombinant microorganism employing a bacterial pathway to produce a cyclic amino acid (e.g., coronamic acid or norcoronamic acid) and a plant enzyme (ACC oxidase) to oxidize the amino acid and produce an alkene (e.g., 1-butene or propene) is provided herein. Expression of these two biosynthetic modules in various microbial chassis will facilitate alkene production from diverse energy and carbon sources, including sugars, glycerol, CO2, CH4, H2, and sunlight.
摘要翻译：提供了使用细菌途径产生环状氨基酸的重组微生物（例如，冠状氨酸或诺卡诺酸）和植物酶（ACC氧化酶）以氧化氨基酸并产生烯烃（例如1-丁烯或丙烯）这里。这些两个生物合成模块在各种微生物底盘中的表达将促进来自不同能量和碳源（包括糖，甘油，二氧化碳，CH4，H2和阳光）的烯烃生产。

4. 发明授权

US07709666B2 Transgenic plants containing altered levels of sterol compounds and tocopherols 有权
标题翻译：含有改变水平的固醇化合物和生育酚的转基因植物
公开(公告)号：US07709666B2
公开(公告)日：2010-05-04
申请号：US10647517
申请日：2004-01-16
申请人： Mylavarapu Venkatramesh , David R. Corbin , B. Ganesh Bhat , Sekhar S. Boddupalli , Robert J. Grebenok , Ganesh M. Kishore , Kathryn D. Lardizabal , Michael W. Lassner , Shaukat H. Rangwala , Balasulojini Karunanandaa
发明人： Mylavarapu Venkatramesh , David R. Corbin , B. Ganesh Bhat , Sekhar S. Boddupalli , Robert J. Grebenok , Ganesh M. Kishore , Kathryn D. Lardizabal , Michael W. Lassner , Shaukat H. Rangwala , Balasulojini Karunanandaa
IPC分类号： C07C59/147
CPC分类号： C12N9/001 , C12N15/8243
摘要： Provided are recombinant constructs comprising DNA sequences encoding enzymes effective in altering the biosynthesis and accumulation of sterol compounds and tocopherols in transgenic plants. Also provided are methods of using such constructs to produce transgenic plants, seeds of which contain elevated levels of sitostanol and/or sitostanol esters, and α-tocopherol, as well as reduced levels of campesterol and campestanol and their corresponding esters. These seeds also contain the novel sterol brassicastanol. Oil obtained from seeds of such transgenic plants is also provided. This oil can be used to prepare food and pharmaceutical compositions effective in lowering the level of low density lipoprotein cholesterol in blood serum. In addition, novel DNA sequences encoding plant steroid 5α-reductases are also disclosed.
摘要翻译：提供了包含编码有效改变转基因植物中甾醇化合物和生育酚的生物合成和积累的酶的DNA序列的重组构建体。还提供了使用这种构建体产生转基因植物的方法，其种子含有升高水平的谷甾烷醇和/或谷甾烷醇酯和α-生育酚，以及降低水平的菜油甾醇和菜油甾醇及其相应的酯。这些种子还含有新的甾醇黄豆糖醇。还提供了从这种转基因植物的种子获得的油。该油可用于制备有效降低血清中低密度脂蛋白胆固醇水平的食品和药物组合物。此外，还公开了编码植物类固醇5α-还原酶的新型DNA序列。

5. 发明授权

US07195917B2 Methods for controlling gibberellin levels 有权
标题翻译：控制赤霉素水平的方法
公开(公告)号：US07195917B2
公开(公告)日：2007-03-27
申请号：US10401321
申请日：2003-03-27
申请人： Sherri M. Brown , Tedd D. Elich , Gregory R. Heck , Ganesh M. Kishore , Eugene W. Logusch , Sherry J. Logusch , Kenneth J. Piller , Sudabathula Rao , Joel E. Ream , Scott R. Baerson
发明人： Sherri M. Brown , Tedd D. Elich , Gregory R. Heck , Ganesh M. Kishore , Eugene W. Logusch , Sherry J. Logusch , Kenneth J. Piller , Sudabathula Rao , Joel E. Ream , Scott R. Baerson
IPC分类号： C12N15/82 , A01H5/00 , C12N5/10
CPC分类号： C12N9/0071 , C12N9/00 , C12N9/0069 , C12N15/8222 , C12N15/8243 , C12N15/8297
摘要： Methods and materials are disclosed for the inhibition and control of gibberellic acid levels. In particular, nucleic acid sequences of copalyl diphosphate synthase. 3-β hydroxylase, and 2-oxidase and additional nucleic acid sequences are disclosed. Gibberellic acid levels may be inhibited or controlled by preparation of a chimeric expression construct capable of expressing a RNA or protein product which suppresses the gibberellin biosynthetic pathway sequence, diverts substrates from the pathway or degrades pathway substrates or products. The sequence is preferably a copalyl diphosphate synthase sequence, a 3β-hydroxylase sequence, a 2-oxidase sequence, a phytoene synthase sequence, a C20-oxidase sequence, and a 2β,3β-hydroxylase sequence. Administration of a complementing agent, preferably a gibberellin or gibberellin precursor or intermediate restores bioactivity.
摘要翻译：公开了用于抑制和控制赤霉酸水平的方法和材料。具体地，涉及椰油二磷酸合成酶的核酸序列。 3-β羟化酶和2-氧化酶和另外的核酸序列。通过制备能够表达抑制赤霉素生物合成途径序列的RNA或蛋白质产物的嵌合表达构建体可以抑制或控制赤霉酸水平，从而使基质从途径转移或降解途径底物或产物。该序列优选为双酰基二磷酸合成酶序列，3β-羟化酶序列，2-氧化酶序列，八氢番茄红素合成酶序列，C20-氧化酶序列和2beta3β-羟化酶序列。补充剂，优选赤霉素或赤霉素前体或中间体的施用恢复生物活性。

6. 发明授权

US07067647B2 Nucleic acid sequences to proteins involved in isoprenoid synthesis 有权
公开(公告)号：US07067647B2
公开(公告)日：2006-06-27
申请号：US09987025
申请日：2001-11-13
申请人： Albert Boronat , Narciso Campos , Ganesh M. Kishore
发明人： Albert Boronat , Narciso Campos , Ganesh M. Kishore
IPC分类号： C12N15/29 , C12N15/61 , C12N15/82 , A01H5/10
CPC分类号： C12N9/0006 , A23D9/007 , A23K20/174 , C11B3/001 , C11B3/12 , C12N9/1085 , C12N9/88 , C12N15/8243 , C12N15/8247 , C12P17/06
摘要： Methods are provided for producing plants and seeds having altered isoprenoid content and compositions. The methods find particular use in increasing the isoprenoid levels in plants, and in providing desirable isoprenoid compositions in a host plant cell.

7. 再颁专利

USRE38825E1 Glyphosate tolerant plants 有权
标题翻译：草甘膦耐受性植物
公开(公告)号：USRE38825E1
公开(公告)日：2005-10-11
申请号：US09612404
申请日：2000-07-07
申请人： Gerard F. Barry , Ganesh M. Kishore
发明人： Gerard F. Barry , Ganesh M. Kishore
IPC分类号： C07H21/04 , C12N1/20 , C12N9/00 , C12N9/02 , C12N9/06 , C12N15/82 , C12P21/06
CPC分类号： C12N9/0012 , C12N9/0004 , C12N15/8275 , C12R1/01 , C12R1/38
摘要： Genes encoding a glyphosate oxidoreductase enzyme are disclosed. The genes are useful in producing transformed bacteria and plants which degrade glyphosate herbicide as well as crop plants which are tolerant to glyphosate herbicide.
摘要翻译：公开了编码草甘膦氧化还原酶的基因。这些基因可用于生产转化的细菌和植物，其降解草甘膦除草剂以及对草甘膦除草剂具有耐受性的作物植物。

8. 发明授权

US06822142B2 Transgenic plants containing altered levels of steroid compounds 有权
标题翻译：含有改变水平的类固醇化合物的转基因植物
公开(公告)号：US06822142B2
公开(公告)日：2004-11-23
申请号：US09885723
申请日：2001-06-20
申请人： Balasulojini Karunanandaa , Martha Post-Beittenmiller , Mylavarapu Venkatramesh , Ganesh M. Kishore , Gregory M. Thorne , John R. LeDeaux
发明人： Balasulojini Karunanandaa , Martha Post-Beittenmiller , Mylavarapu Venkatramesh , Ganesh M. Kishore , Gregory M. Thorne , John R. LeDeaux
IPC分类号： C12N1509
CPC分类号： C12N9/0073 , C12N9/0006 , C12N9/0083 , C12N9/1007 , C12N15/8243 , C12N15/8247 , C12Y101/01034 , C12Y114/99007
摘要： Disclosed are constructs comprising sequences encoding 3-hydroxy-3methylglutaryl-Coenzyme A reductase and at least one other sterol synthesis pathway enzyme. Also disclosed are methods for using such constructs to alter sterol production and content in cells, plants, seeds and storage organs of plants. Also provided are oils and compositions containing altered sterol levels produced by use of the disclosed constructs. Novel nucleotide sequences useful in the alteration of sterol production are also provided. Also provided are cells, plants, seeds and storage organs of plants comprising sequences encoding 3-hydroxy-3methylglutaryl-Coenzyme A reductase, at least one other sterol synthesis pathway enzyme and at least one tocopherol synthesis enzyme.
摘要翻译：公开了包含编码3-羟基-3甲基戊二酰辅酶A还原酶和至少一种其它甾醇合成途径酶的序列的构建体。还公开了使用这种构建体来改变植物的细胞，植物，种子和储存器官中的固醇产生和含量的方法。还提供了含有通过使用所公开的构建体产生的改变的固醇水平的油和组合物。还提供了可用于改变甾醇生产的新型核苷酸序列。还提供了包含编码3-羟基-3甲基戊二酰辅酶A还原酶，至少一种其它甾醇合成途径酶和至少一种生育酚合成酶的序列的植物的细胞，植物，种子和储存器官。

9. 发明授权

US5608149A Enhanced starch biosynthesis in tomatoes 失效
标题翻译：番茄增强淀粉生物合成
公开(公告)号：US5608149A
公开(公告)日：1997-03-04
申请号：US398627
申请日：1995-03-03
申请人： Gerard F. Barry , Ganesh M. Kishore , David M. Stark
发明人： Gerard F. Barry , Ganesh M. Kishore , David M. Stark
IPC分类号： A01H5/00 , C12N5/10 , C12N9/12 , C12N15/09 , C12N15/29 , C12N15/31 , C12N15/54 , C12N15/82 , C12P19/04 , C12N5/04 , C12N15/62
CPC分类号： C12N9/1241 , C12N15/8222 , C12N15/8235 , C12N15/8245 , C12N15/8247
摘要： Promoters for enhanced expression of ADPglucose pyrophosphorylase in potato tubers and fruits such as tomato; methods of using them; DNA molecules, plant cells and plants containing them. A method of decreasing the oil content of seeds by expression of ADPglucose pyrophosphorylase.
摘要翻译：用于增强马铃薯块茎和水果如番茄中ADP葡萄糖焦磷酸化酶表达的启动子; 使用它们的方法; DNA分子，植物细胞和含有它们的植物。通过ADP葡萄糖焦磷酸化酶的表达降低种子含油量的方法。

10. 发明授权

US4562151A Stabilization of L-phenylalanine ammonia-lyase enzyme 失效
标题翻译： L-苯丙氨酸氨裂解酶的稳定化
公开(公告)号：US4562151A
公开(公告)日：1985-12-31
申请号：US529434
申请日：1983-09-06
申请人： Ganesh M. Kishore
发明人： Ganesh M. Kishore
IPC分类号： C12N9/88 , C12N9/96 , C12N11/10 , C12P13/22 , C12R1/645 , C12R1/91 , C12P13/00 , C12P13/06
CPC分类号： C12N11/10 , C12N9/88 , C12N9/96 , C12P13/222
摘要： A process for the synthesis of L-phenylalanines and analogues thereof from trans-cinnamate, ring substituted trans-cinnamates or various acrylate derivatives and ammonia or an ammonia donor in the presence of L-phenylalanine ammonia-lyase enzyme and a desensitizing agent such as polyhydric alcohols and polyethylene glycol-(400) is disclosed. The process embraces the discovery that compounds such as polyhydric alcohols and polyethylene glycol-(400) desensitize the L-phenyalanine ammonia-lyase enzyme to substantially higher substrate concentrations than practiced heretofore. In addition, polyhydric alcohols and polyethylene glycol-(400) substantially enhance the instantaneous rate of reaction and inhibit inactivation of the L-phenylalanine ammonia lyase enzyme over longer reaction periods.
摘要翻译：在L-苯丙氨酸氨裂解酶和脱敏剂如多羟基化合物存在下，从反式肉桂酸酯，环取代的反式肉桂酸酯或各种丙烯酸酯衍生物和氨或氨供体合成L-苯丙氨酸及其类似物的方法醇和聚乙二醇 - （400）。该方法包括以下发现：诸如多元醇和聚乙二醇 - （400）的化合物使L-苯丙氨酸氨裂解酶脱敏至比迄今为止实质上更高的底物浓度。此外，多元醇和聚乙二醇（400）在更长的反应时间内基本上增强了瞬时反应速率并抑制了L-苯丙氨酸氨裂解酶的失活。

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