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    • 3. 发明授权
    • Fluorescent detection of proteins in polyacrylamide gels
    • 聚丙烯酰胺凝胶中蛋白质的荧光检测
    • US08007646B2
    • 2011-08-30
    • US12505859
    • 2009-07-20
    • Robert A. EdwardsRaymond J. TurnerCarol LadnerJean StarkeyEric LarsonDmitri Kazmin
    • Robert A. EdwardsRaymond J. TurnerCarol LadnerJean StarkeyEric LarsonDmitri Kazmin
    • G01N27/453
    • G01N33/6827
    • The mechanism of the UV light-induced reaction between the indole moiety of tryptophan and chloroform, and the structure of the modified tryptophan and polypeptides including such modified tryptophan residues. The excited indole moiety, which is formed upon UV light irradiation, emits a solvated electron which initiates a series of events that yield fluorescent derivatives that have CHO group covalently bound to the indole moiety. These derivatives are herein referred to as formyltryptophan, and are relatively stable. Similar reactions are observed when 5-hydroxytryptophan, 5-fluorotryptophan, or N-methylindolacetate are used in place of tryptophan, or when other haloalkanes, such as trichloracetic acid, trichlorethanol, trichlorethane, bromoform, and iodoactetate are used in place of chloroform. The derivatives can be used in a variety of applications in fluorescence spectroscopy, and for nuclear magnetic resonance, X-ray crystallography, infra-red spectroscopy, circular dicroism and mass spectroscopy. Additionally, the UV light-induced reaction between the indole moiety of tryptophan and haloalkanes can be used to prepare derivatives of tryptophan for chemical cross-linking studies of proteins and peptides.
    • 色氨酸和氯仿的吲哚部分之间的UV光诱导反应的机理,以及修饰的色氨酸和多肽的结构,包括这些修饰的色氨酸残基。 在UV光照射下形成的激发的吲哚部分发射溶剂化电子,其引发一系列事件,其产生具有与吲哚部分共价结合的CHO基团的荧光衍生物。 这些衍生物在本文中称为甲酰色氨酸,并且相对稳定。 当使用5-羟色氨酸,5-氟色氨酸或N-甲基吲哚乙酸乙酯代替色氨酸时,或当使用其它卤代烷烃如三氯乙酸,三氯乙醇,三氯乙烷,溴仿和碘代酸酯代替氯仿时,可以观察到类似的反应。 衍生物可用于荧光光谱学,核磁共振,X射线晶体学,红外光谱,圆形微球和质谱等多种应用。 此外,色氨酸和卤代烷烃的吲哚部分之间的紫外光诱导反应可用于制备色氨酸的衍生物用于蛋白质和肽的化学交联研究。
    • 4. 发明申请
    • Fry basket
    • 炒篮子
    • US20070214968A1
    • 2007-09-20
    • US11384893
    • 2006-03-20
    • Eric LarsonEdward EatonEugene DiMonte
    • Eric LarsonEdward EatonEugene DiMonte
    • A47J37/12
    • A47J37/1295
    • A fry basket includes an open top basket frame having a plurality of upright side walls defining a frying space therewithin. One side of the basket frame is positionable on a support structure above a food receiving station. At least one perforate trap door is movably mounted on the basket frame for movement between a closed position closing a drop opening at the bottom of the frame and an open position to allow cooked food to drop through the opening onto the food receiving station. Actuating means are provided for automatically moving the trap door from its closed to its open position simply in response to moving the basket frame relative to the support structure.
    • 油炸篮包括开放的顶部篮框架,其具有限定其中的油炸空间的多个直立侧壁。 篮框架的一侧可定位在食物接收台上方的支撑结构上。 至少一个穿孔捕捉门可移动地安装在篮架上,用于在关闭位于框架底部的落下开口的关闭位置和打开位置之间移动,以允许熟食通过开口落入食物接收站。 提供致动装置,用于响应于相对于支撑结构移动篮架,自动将收集门从其关闭位置移动到其打开位置。