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1. 发明申请

US20070275464A1 Chitin Oligosaccharide Elicitor-Binding Proteins 审中-公开
标题翻译：甲壳素寡糖诱导剂结合蛋白
公开(公告)号：US20070275464A1
公开(公告)日：2007-11-29
申请号：US10591576
申请日：2005-03-02
申请人： Hanae Kaku , Naoto Shibuya , Eiichi Minami , Naoko Minami , Yoko Nishizawa , Koji Takio , Naoshi Dohmae
发明人： Hanae Kaku , Naoto Shibuya , Eiichi Minami , Naoko Minami , Yoko Nishizawa , Koji Takio , Naoshi Dohmae
IPC分类号： A01H5/00 , A01N43/04 , C07H21/04 , C07K14/00 , C12N15/00 , C12N15/87 , C12N5/00
CPC分类号： C07K14/415 , A01N65/40 , C07K16/16 , C12N15/8218
摘要： The present inventors isolated and purified elicitor-binding proteins in good yield by combining the development of a column that uses APEA derivatives, pre-columns to remove non-specifically adsorbing substances, and effective elution methods. Using the N-terminal and internal chain amino acid sequences of the obtained proteins, the present inventors successfully isolated cDNAs encoding the proteins of the present invention from a rice cDNA library. Moreover, when anti-Con A-CEBiP antibodies were purified and their effect on elicitor-responsive reactive oxygen production was examined, production of reactive oxygen was inhibited by a pretreatment with the antibodies, suggesting that the present proteins are receptor proteins involved in chitin oligosaccharide elicitor responses. Since these elicitors induce resistance to blast in rice, the proteins of the present invention can be applied to the development of novel disease control technologies.
摘要翻译：本发明人通过结合使用APEA衍生物的柱的发展，预柱除去非特异性吸附物质和有效的洗脱方法，以良好的产率分离并纯化了诱导物结合蛋白。使用所得蛋白质的N末端和内部链氨基酸序列，本发明人成功地从水稻cDNA文库中分离了编码本发明蛋白质的cDNA。此外，当抗Con A-CEBiP抗体被纯化并且检测其对诱导因子反应性活性氧产生的作用时，通过用抗体预处理抑制活性氧的产生，表明本发明的蛋白质是参与几丁质寡糖的受体蛋白诱发反应。由于这些诱导剂诱导稻瘟病抗性，因此本发明的蛋白质可用于开发新的疾病控制技术。

2. 发明授权

US06617424B2 Undecapeptides containing cysteinesulfinic and cysteinesulfenic acid 失效
标题翻译：含有半胱氨酸和半胱氨酸的十一肽
公开(公告)号：US06617424B2
公开(公告)日：2003-09-09
申请号：US09805548
申请日：2001-03-14
申请人： Isao Endo , Masafumi Odaka , Masafumi Yohda , Masayoshi Nakasako , Koji Takio
发明人： Isao Endo , Masafumi Odaka , Masafumi Yohda , Masayoshi Nakasako , Koji Takio
IPC分类号： C07K706
CPC分类号： C07K7/06
摘要： Disclosed are peptides represented by IVC1SLC2SC3TAW and C1SLC2SC3 wherein I stands for isoleucine, V for valine, C1 for cysteine, C2 for cysteinesulfinic acid, C3 for cysteinesulfenic acid, S for serine, L for leucine, T for threonine, A for alanine, and W for tryptophan. These peptides can impart photoreactivity to a protein by binding to a non-heme iron. Also disclosed are methods for imparting photoreactivity to cells by introducing the peptide sequences into at least one protein which is involved in a metabolic system and/or energy metabolic system of the cells. There can be provided peptide sequences with a shorter peptide chain capable of imparting photoreactivity, which can be easily introduced into a protein with a little risk in degrading original function of the protein. There are also provided methods enabling control of metabolic reactions by imparting photoreactivity to cells with the peptides.
摘要翻译：公开的是由IVC1SLC2SC3TAW和C1SLC2SC3表示的肽，其中I代表异亮氨酸，V代表缬氨酸，C1代表半胱氨酸，C2代表半胱氨酸，C3代表半胱氨酸，S代表丝氨酸，L代表亮氨酸，T代表苏氨酸，A代表丙氨酸，W 用于色氨酸。这些肽可以通过结合非血红素铁赋予蛋白质光反应性。还公开了通过将肽序列引入参与细胞的代谢系统和/或能量代谢系统的至少一种蛋白质来赋予细胞光反应性的方法。可以提供具有能够赋予光反应性的较短肽链的肽序列，其可以容易地引入蛋白质中，在降解蛋白质的原始功能方面几乎没有风险。还提供了通过赋予细胞与肽的光反应性来控制代谢反应的方法。

3. 发明授权

US06268475B1 Hexapeptides containing cysteinsulfinic and cysteinesulfenic acid 失效
标题翻译：含有半胱氨酰亚砜和半胱氨酸的六肽
公开(公告)号：US06268475B1
公开(公告)日：2001-07-31
申请号：US09113312
申请日：1998-07-10
申请人： Isao Endo , Masafumi Odaka , Masafumi Yohda , Masayoshi Nakasako , Koji Takio
发明人： Isao Endo , Masafumi Odaka , Masafumi Yohda , Masayoshi Nakasako , Koji Takio
IPC分类号： C07K706
CPC分类号： C07K7/06
摘要： Disclosed are peptides represented by IVC1SLC2SC3TAW and C1SLC2SC3 wherein I stands for isoleucine, V for valine, C1 for cysteine, C2 for cysteinesulfinic acid, C3 for cysteinesulfenic acid, S for serine, L for leucine, T for threonine, A for alanine, and W for tryptophan. These peptides can impart photoreactivity to a protein by binding to a non-heme iron. Also disclosed are methods for imparting photoreactivity to cells by introducing the peptide sequences into at least one protein which is involved in a metabolic system and/or energy metabolic system of the cells. There can be provided peptide sequences with a shorter peptide chain capable of imparting photoreactivity, which can be easily introduced into a protein with a little risk in degrading original function of the protein. There are also provided methods enabling control of metabolic reactions by imparting photoreactivity to cells with the peptides.
摘要翻译：公开的是由IVC1SLC2SC3TAW和C1SLC2SC3表示的肽，其中I代表异亮氨酸，V代表缬氨酸，C1代表半胱氨酸，C2代表半胱氨酸，C3代表半胱氨酸，S代表丝氨酸，L代表亮氨酸，T代表苏氨酸，A代表丙氨酸，W 用于色氨酸。这些肽可以通过结合非血红素铁赋予蛋白质光反应性。还公开了通过将肽序列引入参与细胞的代谢系统和/或能量代谢系统的至少一种蛋白质来赋予细胞光反应性的方法。可以提供具有能够赋予光反应性的较短肽链的肽序列，其可以容易地引入蛋白质中，在降解蛋白质的原始功能方面几乎没有风险。还提供了通过赋予细胞与肽的光反应性来控制代谢反应的方法。

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