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    • 10. 发明授权
    • Flavonoid-3′,5′-hydroxylase gene of commelina communis
    • 黄瓜类黄酮3',5'-羟化酶基因
    • US08440879B2
    • 2013-05-14
    • US12530497
    • 2008-03-13
    • Shunji YukiSatoshi ArakiTakanori Suzuki
    • Shunji YukiSatoshi ArakiTakanori Suzuki
    • C12N15/29C12N15/82C12N5/10A01H5/00
    • C12N15/825C12N9/0073
    • To provide a highly expressed F3′5′H gene, whereby a blue moth orchid can be produced and a method using the gene for producing Orchids having blue flowers.A gene encoding a flavonoid 3′,5′-hydroxylase of Commelina communis, which has an amino acid sequence depicted in SEQ ID No: 2 or an amino acid sequence having at least 90% of homology to the amino acid sequence depicted in SEQ ID No: 2. A method for producing an orchid having a blue flower, which comprises transfecting a white moth orchid with the gene, a gene encoding a dihydroflavonol 4-reductase of Torenia or Gerbera; a gene encoding a flavanone 3-hydroxylase; and a gene encoding an anthocyanidin synthase and expressing the genes.
    • 为了提供高度表达的F3'5'H基因,由此可以产生蓝色蛾兰花,以及使用该蓝根基因的方法。 编码Commelina communis的黄酮3',5'-羟化酶的基因,其具有SEQ ID No:2所示的氨基酸序列或与SEQ ID NO:1所示氨基酸序列具有至少90%同源性的氨基酸序列 No:2.一种生产具有蓝色花的兰花的方法,其包括用该基因转染白色蛾兰,编码Torenia或非洲菊的二氢黄酮醇4-还原酶的基因; 编码黄烷酮3-羟化酶的基因; 和编码花色素合成酶并表达基因的基因。