专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

1. 发明申请

US20210024624A1 FUSION PROTEINS FOR OPHTHALMOLOGY WITH INCREASED EYE RETENTION 有权
公开(公告)号：US20210024624A1
公开(公告)日：2021-01-28
申请号：US17066215
申请日：2020-10-08
申请人： Hoffmann-La Roche Inc.
发明人： Michael HUELSMANN , Erhard KOPETZKI
IPC分类号： C07K16/18 , A61K47/65 , A61K47/68 , A61P27/02 , C07K16/46
摘要： The combination of a first binding site specifically binding to a target associated with an eye disease and a second binding site specifically binding to a target influencing the retention in the eye a multispecific binder provides for improved intravitreal retention compared to a monospecific binder. The second binding site specifically binds to a compound/molecules found in the extracellular matrix (ECM) in vitreous humor/retina. This compound of the extracellular matrix has to be present in amounts allowing a sufficient loading/dose of the drug to be bound. It has been found that collagen, especially collagen II, is a suitable compound in the ECM in the vitreous humor for this purpose. Thus, herein is reported a multispecific binder comprising a first binding site specifically binding to a therapeutic ocular target, and a second binding site specifically binding to collagen II.

2. 发明申请

US20150246961A1 METHOD FOR THE EXPRESSION OF POLYPEPTIDES USING MODIFIED NUCLEIC ACIDS 审中-公开
标题翻译：使用改性核酸表达多肽的方法
公开(公告)号：US20150246961A1
公开(公告)日：2015-09-03
申请号：US14517516
申请日：2014-10-17
申请人： HOFFMANN-LA ROCHE INC.
发明人： Stefan KLOSTERMANN , Erhard KOPETZKI , Ursula SCHWARZ
IPC分类号： C07K16/00
CPC分类号： C07K16/00 , C07K2317/14 , C12N15/67
摘要： Herein is reported a method for recombinantly producing a polypeptide in a cell comprising the step of cultivating a cell which comprises a nucleic acid encoding the polypeptide, and recovering the polypeptide from the cell or the cultivation medium, wherein each of the amino acid residues of the polypeptide is encoded by at least one codon, whereby the different codons encoding the same amino acid residue are combined in one group and each of the codons in a group is defined by a specific usage frequency within the group, whereby the sum of the specific usage frequencies of all codons in one group is 100%, and wherein the usage frequency of a codon in the polypeptide encoding nucleic acid is about the same as its specific usage frequency within its group.
摘要翻译：本文报道了一种在细胞中重组产生多肽的方法，包括培养包含编码该多肽的核酸的细胞的步骤，以及从该细胞或培养基中回收多肽，其中每个氨基酸残基多肽由至少一个密码子编码，由此将编码相同氨基酸残基的不同密码子组合在一组中，组中的每个密码子由组内的特定使用频率定义，由此具体用途的总和一组中所有密码子的频率为100％，其中编码多肽的核酸中的密码子的使用频率与其组内的特定使用频率大致相同。

3. 发明申请

US20190233842A1 PROTEIN EXPRESSION FROM MULTIPLE NUCLEIC ACIDS 审中-公开
公开(公告)号：US20190233842A1
公开(公告)日：2019-08-01
申请号：US16107929
申请日：2018-08-21
申请人： HOFFMANN-LA ROCHE INC.
发明人： Ulrich GOEPFERT , Hendrik KNOETGEN , Erhard KOPETZKI , Anne STERN
IPC分类号： C12N15/85 , C12P21/00 , C07K16/28 , C07K16/18
CPC分类号： C12N15/85 , C07K16/18 , C07K16/28 , C07K16/2812 , C07K16/2854 , C07K16/2866 , C07K16/2896 , C07K2317/14 , C07K2317/21 , C07K2317/51 , C07K2317/515 , C12P21/00
摘要： The current invention reports a method for the recombinant production of a secreted heterologous immunoglobulin in a CHO cell comprising the following steps: i) providing a CHO cell, which is adapted to growth in suspension culture, adapted to growth in serum-free medium, mycoplasma free, and virus free, ii) providing a vector comprising a prokaryotic origin of replication, a first nucleic add conferring resistance to a prokaryotic selection agent, a second nucleic acid encoding the heavy chain of said heterologous immunoglobulin, a third nucleic acid encoding the light chain of said heterologous immunoglobulin, a fourth nucleic acid conferring resistance to a eukaryotic selection agent, iii) transfecting said CHO cell, wherein said transfecting comprises a) transfecting said CHO cell with said vector comprising a fourth nucleic acid conferring resistance to a first eukaryotic selection agent, h) selecting a CHO cell by growth in cultivation medium containing said first eukaryotic selection agent, c) transfecting said selected CHO cell with said vector comprising a fourth nucleic acid conferring resistance to a second eukaryotic selection agent different to said first eukaryotic selection agent, d) selecting a CHO cell by selected growth in cultivation medium containing said first and said second eukaryotic selection agent, iv) cultivating said transfected CHO cell in a medium in the presence of said first and second eukaryotic selection agent, under conditions suitable for the expression of said second, and third nucleic acid, and v) recovering said secreted heterologous immunoglobulin from the cultivation medium.

4. 发明申请

US20190002570A1 METHOD FOR SELECTION AND PRODUCTION OF TAILOR-MADE HIGHLY SELECTIVE AND MULTI-SPECIFIC TARGETING ENTITIES CONTAINING AT LEAST TWO DIFFERENT BINDING ENTITIES AND USES THEREOF 审中-公开
公开(公告)号：US20190002570A1
公开(公告)日：2019-01-03
申请号：US16133579
申请日：2018-09-17
申请人： Hoffmann-La Roche Inc.
发明人： Sebastian FENN , Erhard KOPETZKI , Georg TIEFENTHALER
IPC分类号： C07K16/28 , C07K16/40
摘要： Herein is reported a method for producing a bispecific antibody comprising the step of incubating (i) an antibody Fab fragment or a scFv antibody comprising within the 20 C-terminal amino acid residues the amino acid sequence LPX1TG (SEQ ID NO: 01), (ii) a one-armed antibody comprising a full length antibody heavy chain, a full length antibody light chain, and an Fc-heavy chain, whereby the full length antibody heavy chain and the full length antibody light chain are cognate antibody chains that thereof forms an antigen binding site, whereby the full length antibody heavy chain and the Fc-heavy chain are covalently linked to each other via one or more disulfide bonds forming an antibody hinge region, and whereby the Fc-heavy chain has an oligoglycine amino acid sequence at its N-terminus, and (iii) a Sortase A enzyme.

5. 发明申请

US20190271021A1 RECOMBINANT POLYPEPTIDE PRODUCTION METHODS 审中-公开
公开(公告)号：US20190271021A1
公开(公告)日：2019-09-05
申请号：US16413063
申请日：2019-05-15
申请人： Hoffmann-La Roche Inc.
发明人： Erhard KOPETZKI , Jens NIEWOEHNER , Peter MAIER
IPC分类号： C12P21/00 , C12P21/02 , C07K5/11 , C07K14/475 , C07K16/28 , C07K14/48
摘要： Herein is reported a method for producing a fusion-polypeptide comprising the seeps of a) cultivating a mammalian cell comprising a nucleic acid encoding a variant fusion-polypeptide wherein the amino acid sequence of the fusion-polypeptide has been modified by replacing in a pro-fusion-polypeptide the endogenous protease cleavage site between the pro-peptide and the fusion-polypeptide with an exogenous (with respect to the origins of the parts of the fusion-polypeptide) or artificial protease cleavage site, and b) recovering the fusion-polypeptide or fusion-pro-polypeptide from the cell or the cultivation median and thereby producing the (recombinant) fusion-polypeptide.

6. 发明申请

US20160326562A1 IMPROVED RECOMBINANT POLYPEPTIDE PRODUCTION METHODS 审中-公开
标题翻译：改进的重组多肽生产方法
公开(公告)号：US20160326562A1
公开(公告)日：2016-11-10
申请号：US15106205
申请日：2014-12-10
申请人： HOFFMANN-LA ROCHE INC.
发明人： Erhard KOPETZKI , Jens NIEWOEHNER , Peter MAIER
IPC分类号： C12P21/00 , C07K14/48 , C07K5/11 , C07K14/475 , C07K16/28
CPC分类号： C12P21/005 , C07K5/1019 , C07K14/475 , C07K14/48 , C07K16/2863 , C07K16/2881 , C07K2317/14 , C07K2317/522 , C07K2317/524 , C07K2317/526 , C07K2317/53 , C07K2317/55 , C07K2317/56 , C07K2319/00 , C07K2319/50 , C07K2319/60 , C12P21/02
摘要： Herein is reported a method for producing a fusion-polypeptide comprising the steps of a) cultivating a mammalian cell comprising a nucleic acid encoding a variant fusion-polypeptide wherein the amino acid sequence of the fusion-polypeptide has been modified by replacing in a pro-fusion-polypeptide the endogenous protease cleavage site between the pro-peptide and the fusion-polypeptide with an exogenous (with respect to the origins of the parts of the fusion-polypeptide) or artificial protease cleavage site, and b) recovering the fusion-polypeptide or fusion-pro-polypeptide from the cell or the cultivation medium and thereby producing the (recombinant) fusion-polypeptide.
摘要翻译：本文报道了一种产生融合多肽的方法，包括以下步骤：a）培养包含编码变体融合多肽的核酸的哺乳动物细胞，其中融合多肽的氨基酸序列已被修饰，融合多肽与外源（相对于融合多肽部分的起源）或人造蛋白酶切割位点的前肽和融合多肽之间的内源性蛋白酶切割位点，和b）回收融合多肽或来自细胞或培养基的融合原多肽，从而产生（重组）融合多肽。

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式