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1. 发明授权

US10150953B2 Well-tolerated and highly specific tailored recombinase for recombining asymmetric target sites in a plurality of retrovirus strains 有权
公开(公告)号：US10150953B2
公开(公告)日：2018-12-11
申请号：US15317088
申请日：2015-09-01
申请人： HEINRICH-PETTE-INSTITUT LEIBNIZ-INSTITUT FÜR EXPERIMENTELLE VIROLOGIE-STIFTUNG BÜRGERLICHEN RECHTS , TECHNISCHE UNIVERSITÄT DRESDEN
发明人： Joachim Hauber , Jan Chemnitz , Frank Buchholz , Janet Karpinski
IPC分类号： C12N9/12 , C12N1/20 , C12N15/00 , C07H21/04 , C12P21/06 , C12P21/04 , C12N9/22 , C12N15/10 , A61K38/00
摘要： The present invention relates to a method for preparing an expression vector encoding a well-tolerated and highly specific tailored recombinase, which tailored recombinase is capable of recombining asymmetric target sequences within the long terminal repeat (LTR) of proviral DNA of a plurality of retrovirus strains which may be inserted into the genome of a host cell, as well as to the obtained expression vector, cells transfected with these, expressed recombinase and pharmaceutical compositions comprising the expression vector, cells and/or recombinase. Pharmaceutical compositions are useful, e.g., in treatment and/or prevention of retrovirus infection, in particular, HIV infection. In particular, the invention relates to well-tolerated and highly specific tailored recombinases capable of combining asymmetric target sequences in a more than 90% of HIV-strains, thereby excising the HIV-1 sequences, and expression vectors encoding them.

2. 发明申请

US20170175091A1 WELL-TOLERATED AND HIGHLY SPECIFIC TAILORED RECOMBINASE FOR RECOMBINING ASYMMETRIC TARGET SITES IN A PLURALITY OF RETROVIRUS STRAINS 审中-公开
公开(公告)号：US20170175091A1
公开(公告)日：2017-06-22
申请号：US15317088
申请日：2015-09-01
申请人： Heinrich-Pette-Institut Leibniz-Institut für Experimentelle Virologie - Stiftung bürgerlichen , Technische Universität Dresden
发明人： Joachim Hauber , Jan Chemnitz , Frank Buchholz , Janet Karpinski
IPC分类号： C12N9/12 , C12N15/10
CPC分类号： C12N9/1241 , A61K38/00 , C12N9/22 , C12N15/1058
摘要： The present invention relates to a method for preparing an expression vector encoding a well-tolerated and highly specific tailored recombinase, which tailored recombinase is capable of recombining asymmetric target sequences within the long terminal repeat (LTR) of proviral DNA of a plurality of retrovirus strains which may be inserted into the genome of a host cell, as well as to the obtained expression vector, cells transfected with these, expressed recombinase and pharmaceutical compositions comprising the expression vector, cells and/or recombinase. Pharmaceutical compositions are useful, e.g., in treatment and/or prevention of retrovirus infection, in particular, HIV infection. In particular, the invention relates to well-tolerated and highly specific tailored recombinases capable of combining asymmetric target sequences in a more than 90% of HIV-strains, thereby excising the HIV-1 sequences, and expression vectors encoding them.

3. 发明授权

US09428754B2 Protein with recombinase activity for site-specific DNA-recombination 有权
公开(公告)号：US09428754B2
公开(公告)日：2016-08-30
申请号：US14416997
申请日：2013-07-22
申请人： Technische Universität Dresden
发明人： Frank Buchholz , Madina Karimova
IPC分类号： C12N15/87 , C12N15/52 , C12N9/12 , C12N15/85 , C12N15/90 , C07H21/04 , C12N15/55 , C12N15/63 , C12N15/66
CPC分类号： C12N15/907 , C12N9/12 , C12N9/1241 , C12N15/52 , C12N15/85 , C12N15/902 , C12N2320/33 , C12Y207/07
摘要： The invention relates to the use of a protein with recombinase activity to catalyze a site-specific DNA recombination and a method for producing a site-specific DNA recombination. The invention is applicable alone or in combination with other recombinase systems for genetic manipulation, for example in medical research. The objective of the invention is solved by the use of a protein with recombinase activity to catalyze a site-specific DNA recombination at, preferably at two, recognition sites that are identical or reverse complementary to each other. The invention also includes a method for producing a site-specific DNA recombination comprising the steps of a) providing a cell comprising at least two recognition sites that are identical or reverse complementary to each other; and b) contacting a protein with recombinase activity with the recognition sites, thereby producing the site-specific DNA-recombination.

4. 发明授权

US11845954B2 Methods and means for genetic alteration of genomes utilizing designer DNA recombining enzymes 有权
公开(公告)号：US11845954B2
公开(公告)日：2023-12-19
申请号：US16622937
申请日：2018-06-14
申请人： TECHNISCHE UNIVERSITÄT DRESDEN
发明人： Frank Buchholz , Martin Schneider , Felix Lansing
IPC分类号： C12N15/90 , C12N9/22 , C12N15/10 , C12Q1/6869 , C12N9/00 , C12N9/12
CPC分类号： C12N15/907 , C12N9/22 , C12N15/1058 , C12Q1/6869
摘要： The invention provides methods for specifically altering the DNA sequence in a genome, in particular for genome editing by deleting or replacing a sequence of interest. Advantageously, the invention uses two non-identical sequences naturally occurring in a genome as target sites two which DNA-recombining enzymes are generated. The invention is in particular useful for medicine, in particular to repair a mutation in a genome or to delete predefined genetic material from cells or tissue and to cure diseases. An advantage of the invention is that it allows precise site directed altering of DNA without engaging host DNA repair pathways and thereby works without inducing random insertions and deletions (indels).

5. 发明申请

US20150176012A1 Protein with recombinase activity for site-specific DNA-recombination 有权
标题翻译：具有重组酶活性的蛋白质用于位点特异性DNA重组
公开(公告)号：US20150176012A1
公开(公告)日：2015-06-25
申请号：US14416997
申请日：2013-07-22
申请人： Technische Universität Dresden
发明人： Frank Buchholz , Madina Karimova
IPC分类号： C12N15/52 , C12N9/12
CPC分类号： C12N15/907 , C12N9/12 , C12N9/1241 , C12N15/52 , C12N15/85 , C12N15/902 , C12N2320/33 , C12Y207/07
摘要： The invention relates to the use of a protein with recombinase activity to catalyze a site-specific DNA recombination and a method for producing a site-specific DNA recombination. The invention is applicable alone or in combination with other recombinase systems for genetic manipulation, for example in medical research. The objective of the invention is solved by the use of a protein with recombinase activity to catalyze a site-specific DNA recombination at, preferably at two, recognition sites that are identical or reverse complementary to each other. The invention also includes a method for producing a site-specific DNA recombination comprising the steps of a) providing a cell comprising at least two recognition sites that are identical or reverse complementary to each other; and b) contacting a protein with recombinase activity with the recognition sites, thereby producing the site-specific DNA-recombination.
摘要翻译：本发明涉及具有重组酶活性的蛋白质用于催化位点特异性DNA重组的用途和用于产生位点特异性DNA重组的方法。本发明可单独应用或与用于遗传操作的其他重组酶系统组合应用，例如在医学研究中。本发明的目的是通过使用具有重组酶活性的蛋白质来催化位点特异性DNA重组，优选在两个彼此相同或相互互补的识别位点。本发明还包括用于产生位点特异性DNA重组的方法，包括以下步骤：a）提供包含彼此相同或相互互补的至少两个识别位点的细胞; 和b）使蛋白质与重组酶活性与识别位点接触，从而产生位点特异性DNA重组。

6. 发明授权

US10253332B2 Protein with recombinase activity for site-specific DNA-recombination 有权
公开(公告)号：US10253332B2
公开(公告)日：2019-04-09
申请号：US15634949
申请日：2017-06-27
申请人： Technische Universität Dresden
发明人： Frank Buchholz , Madina Karimova
IPC分类号： C12N15/87 , C12N15/52 , C12N15/90 , C12N9/12 , C12N15/85 , C07H21/04 , C12N15/55 , C12N15/63 , C12N15/66
摘要： The invention relates to the use of a protein with recombinase activity to catalyze a site-specific DNA recombination and a method for producing a site-specific DNA recombination. The invention is applicable alone or in combination with other recombinase systems for genetic manipulation, for example in medical research. The objective of the invention is solved by the use of a protein with recombinase activity to catalyze a site-specific DNA recombination at, preferably at two, recognition sites that are identical or reverse complementary to each other. The invention also includes a method for producing a site-specific DNA recombination comprising the steps of a) providing a cell comprising at least two recognition sites that are identical or reverse complementary to each other; and b) contacting a protein with recombinase activity with the recognition sites, thereby producing the site-specific DNA-recombination.

7. 发明授权

US09719109B2 Protein with recombinase activity for site-specific DNA-recombination 有权
公开(公告)号：US09719109B2
公开(公告)日：2017-08-01
申请号：US15216215
申请日：2016-07-21
申请人： Technische Universität Dresden
发明人： Frank Buchholz , Madina Karimova
IPC分类号： C12N15/87 , C12N15/52 , C12N15/90 , C12N9/12 , C12N15/85 , C07H21/04 , C12N15/55 , C12N15/63 , C12N15/66
CPC分类号： C12N15/907 , C12N9/12 , C12N9/1241 , C12N15/52 , C12N15/85 , C12N15/902 , C12N2320/33 , C12Y207/07
摘要： The invention relates to the use of a protein with recombinase activity to catalyze a site-specific DNA recombination and a method for producing a site-specific DNA recombination. The invention is applicable alone or in combination with other recombinase systems for genetic manipulation, for example in medical research. The objective of the invention is solved by the use of a protein with recombinase activity to catalyze a site-specific DNA recombination at, preferably at two, recognition sites that are identical or reverse complementary to each other. The invention also includes a method for producing a site-specific DNA recombination comprising the steps of a) providing a cell comprising at least two recognition sites that are identical or reverse complementary to each other; and b) contacting a protein with recombinase activity with the recognition sites, thereby producing the site-specific DNA-recombination.

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