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1. 发明申请

US20090023912A1 Water-soluble, uv-absorbing and/or flourescent components with very high isoelectric points 审中-公开
标题翻译：具有非常高等电点的水溶性，紫外线吸收和/或荧光成分
公开(公告)号：US20090023912A1
公开(公告)日：2009-01-22
申请号：US11587080
申请日：2005-04-22
申请人： Gyula Vigh , Sanjiv K. Lalwani , Brent Busby , Kingsley Nzeadibe , Helen C. Fleisher , Evan E. Shave
发明人： Gyula Vigh , Sanjiv K. Lalwani , Brent Busby , Kingsley Nzeadibe , Helen C. Fleisher , Evan E. Shave
IPC分类号： C08B37/16
CPC分类号： G01N27/44747 , C07H1/00 , C08K5/19
摘要： Water-soluble, UV-absorbing and/or fluorescing compound having an isoelectric point greater than 10, can be obtained using molecules chosen from the group of molecules consisting of those containing at least one secondary alcohol OH group with a PKa value greater than 10 and at least one amino group with a pKb value smaller than 5, those containing at least one primary alcohol OH group and at least one secondary alcohol OH group with respective PKa values greater than 10 and at least one amino group with a pKb value smaller than 5 or a quaternary ammonium group, and those containing at least two secondary alcohol OH groups with respective pKa values greater than 10 and at least one amno group with a pKb value smaller than 5 or a quaternary ammonium group; and having at least one UV absorbing group or at least one fluorescing group or a combination thereof. The invention includes methods for producing such compounds.
摘要翻译：具有等电点大于10的水溶性，紫外线吸收和/或荧光化合物可以使用选自包含至少一个具有PKa值大于10的仲醇OH基的那些分子组成的分子， pKb值小于5的至少一个氨基，含有至少一个伯醇OH基团的至少一个氨基和各自PKa值大于10的至少一个仲醇OH基团和至少一个pKb值小于5的氨基或季铵基，以及含有至少两个相应pKa值大于10的仲醇OH基和至少一个pKb值小于5的氨基或季铵基的那些基团; 并具有至少一个UV吸收基团或至少一个荧光基团或其组合。本发明包括生产这种化合物的方法。

2. 发明授权

US07052589B1 Method for electrophoretic separations using dynamically generated opposite mobilities 失效
标题翻译：使用动态生成的相反移动性的电泳分离方法
公开(公告)号：US07052589B1
公开(公告)日：2006-05-30
申请号：US10100549
申请日：2002-03-18
申请人： Gyula Vigh
发明人： Gyula Vigh
IPC分类号： G01N27/447
CPC分类号： G01N27/44769 , G01N27/44747
摘要： Method and materials to carry out preparative-scale electrophoretic separations based on the principle of dynamically created non-co-directional effective electrophoretic mobilities are disclosed. The primary application areas of the method are in the separation, purification, enrichment, concentration or conditioning of both small and large molecular weight, weak and strong electrolyte compounds, such as pharmaceuticals, oligo- and polypeptides, proteins, oligonucleotides, etc. These objectives can be achieved based on the use of a secondary chemical equilibrium, alone or in combination with multiple protic and other secondary chemical equilibria. Though such electrophoretic operations could be achieved by other means, such as by conventional zone electrophoresis or isotachophoresis, the method disclosed here can provide greater separation power, simplicity and higher production rates.
摘要翻译：公开了基于动态创建的非同向有效电泳迁移率原理进行制备规模电泳分离的方法和材料。该方法的主要应用领域是分离，纯化，富集，浓缩或调节小分子量和大分子量，弱和强的电解质化合物，如药物，寡聚和多肽，蛋白质，寡核苷酸等。这些目标可以基于二级化学平衡的使用，单独使用或与多种质子和其他二级化学平衡相结合来实现。尽管这样的电泳操作可以通过其他方式实现，例如通过常规区域电泳或等速电泳，但是这里公开的方法可以提供更大的分离能力，简单性和更高的生产速率。

3. 发明申请

US20060049052A1 Ampholytic buffer having high buffering capacity and high conductivity in isoelectric form 审中-公开
标题翻译：具有高缓冲能力和等电点形式的高导电性的两性缓冲液
公开(公告)号：US20060049052A1
公开(公告)日：2006-03-09
申请号：US11219351
申请日：2005-09-02
申请人： Gyula Vigh , Sanjiv Lalwani
发明人： Gyula Vigh , Sanjiv Lalwani
IPC分类号： C07K1/26 , G01N27/447
CPC分类号： G01N27/44795 , G01N27/44747
摘要： Ampholytic buffers having a high buffering capacity and a high conductivity in the isoelectric state were synthesized by creating molecules in which four or more bonds separate the charge-carrying or chargeable atoms of the pI-determining weak electrolyte functional groups and, simultaneously, the charge-carrying or chargeable atom of the weak or strong electrolyte charge-balancing functional group.
摘要翻译：通过产生其中四个或更多个键分离pI-确定的弱电解质官能团的带电荷或可带电原子的分子，并且同时进行电荷 - 反应，从而合成具有高等缓冲能力和等电点高导电性的两性缓冲液，弱或强电解质电荷平衡官能团的携带或带电原子。

4. 发明授权

US06391862B1 Chiral resolving agents for enantioseparations 有权
标题翻译：手性拆分剂用于对映体分离
公开(公告)号：US06391862B1
公开(公告)日：2002-05-21
申请号：US09172575
申请日：1998-10-14
申请人： Gyula Vigh
发明人： Gyula Vigh
IPC分类号： A61K31715
CPC分类号： C08B37/0012 , C07B57/00 , C07H11/00
摘要： A cyclodextrin composition of substantially pure, sulfated-cyclodextrin derivatives particularly suitable as chiral resolving agents for enantioseparation by electrophoresis. The cyclodextrin composition preferably have an isomeric purity of at least 80 mole %. Non-sulfato substituents for the substantially pure cyclodextrin derivatives are hydrogen, C1-C12 alkyl groups, C2-C8 hydroxyalkyl groups, C1-C12 alkylnitryl groups, C2-C12 acyl groups, aryl groups, carbamate groups, thiocarbamate groups or combinations thereof.
摘要翻译：基本上纯的硫酸化环糊精衍生物的环糊精组合物，特别适用作通过电泳对映体分离的手性拆分剂。环糊精组合物优选具有至少80摩尔％的异构纯度。基本上纯的环糊精衍生物的非硫酸酯取代基是氢，C 1 -C 12烷基，C 2 -C 8羟基烷基，C 1 -C 12烷基硝基，C 2 -C 12酰基，芳基，氨基甲酸酯基，硫代氨基甲酸酯基或其组合。

5. 发明申请

US20080264793A1 Hydrolytically Stable Isoelectric Hydrogel Compositions 审中-公开
标题翻译：水解稳定的等电泳水凝胶组合物
公开(公告)号：US20080264793A1
公开(公告)日：2008-10-30
申请号：US10567743
申请日：2004-08-13
申请人： Gyula Vigh , Sanjiv Kumar S. Lalwani
发明人： Gyula Vigh , Sanjiv Kumar S. Lalwani
IPC分类号： B01D59/42
CPC分类号： G01N27/44747 , B01D61/425 , B01D71/38 , C07K1/24 , C08F8/00 , C08F8/14 , G01N27/44795 , C08F16/06
摘要： An hydrolytically stable isoelectric hydrogel material comprising a single isoelectric compound having a defined pI value from 1 to 12 being incorporated into a hydrogel formed by reacting an oligo- or polyhydroxy compound with the single iso-electric compound and a difunctional or oligofunctional crosslinker, wherein after incorporation of the single isoelectric compound into the hydrogel, the hydrogel material become's an ampholytic material.
摘要翻译：包含具有1至12的限定pI值的单一等电点化合物的水解稳定的等电点水凝胶材料被引入通过使低聚或多羟基化合物与单一异电化合物和双官能或低官能交联剂反应而形成的水凝胶中，其中将单一等电点化合物引入水凝胶中，水凝胶材料成为两性溶解材料。

6. 发明申请

US20060151325A1 Method for ph-biased isoelectric trapping separations 审中-公开
标题翻译： ph偏置等电俘获分离方法
公开(公告)号：US20060151325A1
公开(公告)日：2006-07-13
申请号：US10516734
申请日：2003-06-03
申请人： Gyula Vigh
发明人： Gyula Vigh
IPC分类号： C07K1/26 , B01D57/02
CPC分类号： G01N27/44795 , B01D57/02 , C07K1/26
摘要： A method for separating an ampholytic component by electrophoresis, the method involving placing a sample containing an ampholytic component having a pI value in an electrophoresis separation system comprising an anolyte having a pH and a catholyte having a pH, the catholyte pH being higher than the anolyte pH, one or more ion-permeable barriers disposed between the anolyte and catholyte wherein at least one of the barriers is an isoelectric barrier having a pI value which is higher than the anolyte pH and lower than the catholyte pH; providing an isoelectric buffer having a pI value higher than the pH of the anolyte and lower than the pH of the catholyte and different from the pI value of the ampholytic sample component and different from the pI value of an ion-permeable isoelectric barrier; and exposing the sample to an electric potential so as to trap the ampholytic sample component in a non-isoelectric state in the presence of the isoelectric buffer in the electrophoresis system.
摘要翻译：一种通过电泳分离两性成分的方法，所述方法包括将含有pI值的两性成分的样品放置在电泳分离系统中，所述电泳分离系统包括具有pH的阳极电解液和具有pH的阴极电解液，所述阴极电解液pH高于阳极电解液 pH，设置在阳极电解液和阴极电解液之间的一个或多个离子可渗透屏障，其中至少一个屏障是具有高于阳极电解液pH并低于阴极电解液pH的pI值的等电势屏障; 提供pI值高于阳极电解液的pH并且低于阴极电解液的pH并且不同于两性样品组分的pI值并且不同于离子可渗透等电势屏障的pI值的等电点缓冲液; 并将样品暴露于电位，以便在电泳系统中存在等电点缓冲液的情况下将两性样品组分捕获为非等电位状态。

7. 发明授权

US09689841B2 Fluorescent pI markers for isoelectric focusing separations and fluorescent labeling 有权
公开(公告)号：US09689841B2
公开(公告)日：2017-06-27
申请号：US14241358
申请日：2012-08-27
申请人： Gyula Vigh , Ming-Chien Li
发明人： Gyula Vigh , Ming-Chien Li
IPC分类号： C07C311/32 , G01N27/447 , C09K11/06 , C07C311/42 , C07D233/64 , C07D295/088 , C07D295/13 , C07D295/26 , C07C311/37 , G01N33/58
CPC分类号： G01N27/44795 , C07C311/32 , C07C311/37 , C07C311/42 , C07C2603/50 , C07D233/64 , C07D295/088 , C07D295/13 , C07D295/26 , C09K11/06 , C09K2211/1007 , C09K2211/1011 , C09K2211/1033 , C09K2211/1044 , G01N27/447 , G01N33/582 , Y10T436/143333
摘要： UV-Absorbing and fluorescent pl markers for isoelectric focusing separations and fluorescent labeling, and methods for making and using the markers.

8. 发明授权

US06923896B2 Electrophoresis apparatus and method 失效
公开(公告)号：US06923896B2
公开(公告)日：2005-08-02
申请号：US09961591
申请日：2001-09-22
申请人： Gyula Vigh , David Ogle , Dennis Brian Rylatt
发明人： Gyula Vigh , David Ogle , Dennis Brian Rylatt
IPC分类号： G01N27/26 , B01D57/02 , B01D61/46 , B03C5/00 , C12M1/00 , C12M1/34 , G01N27/447 , G01N27/453
CPC分类号： G01N27/44795 , B01D57/02 , G01N27/44769
摘要： An electrophoretic apparatus comprising: a first electrolyte chamber containing a first electrode; a second electrolyte chamber containing a second electrode; a first sample chamber disposed between the first and second electrolyte chambers and proximate to the first electrolyte chamber; a second sample chamber disposed between the first sample chamber and the second electrolyte; three ion-permeable barriers separating the first electrolyte chamber, the first sample chamber, the second sample chamber, and the second electrolyte chamber, respectively, wherein the ion-permeable barriers impede convective mixing of the contents in each of the respective chambers; a first electrolyte reservoir and a second electrolyte reservoir in fluid communication with the first and second electrolyte chambers, respectively; a first sample reservoir and a second sample reservoir in fluid communication with the first and second sample chambers, respectively; means adapted for communicating a first electrolyte and a second electrolyte between the respective electrolyte chambers and reservoirs; means adapted for communicating a first fluid and a second fluid between the respective sample chambers and reservoirs, wherein at least one of the first and second fluid contains at least a sample, wherein application of an electric potential causes migration of at least one component through at least one of the ion-permeable barriers.

9. 发明授权

US09896522B2 Solid phase fluorescence labeling reagents and uses thereof 有权
公开(公告)号：US09896522B2
公开(公告)日：2018-02-20
申请号：US13819668
申请日：2011-08-26
申请人： Gyula Vigh , Roy T. Estrada, III
发明人： Gyula Vigh , Roy T. Estrada, III
IPC分类号： G01N33/58 , C07C311/39 , C08F20/26 , C07D317/22 , G01N33/543 , C09B57/00 , G01N33/68
CPC分类号： C08F20/26 , C07C311/39 , C07C2603/50 , C07D317/22 , C09B57/001 , G01N33/54306 , G01N33/54393 , G01N33/582 , G01N33/6809 , Y10T436/173845
摘要： A solid phase reagent and method for simultaneously capturing and fluorescently labeling an analyte with multiple reactive sites to provide a mono-labeled analyte are disclosed. The reagent can be used in the method and comprises a plurality of analyte-reactive groups tethered to a porous solid phase, wherein each analyte-reactive group is covalently attached to a fluorescent group either directly or indirectly, wherein the distance between adjacent analyte-reactive groups is greater than the gyration radius of a captured analyte; the fluorescent group is covalently attached to a cleavable anchor group either directly or through a first spacer; and the cleavable anchor group is covalently attached to the solid phase either directly or through a second spacer, wherein the solid phase is a porous solid or a porous gel.

10. 发明申请

US20070205106A1 Electrophoresis Apparatus 审中-公开
标题翻译：电泳仪
公开(公告)号：US20070205106A1
公开(公告)日：2007-09-06
申请号：US10576939
申请日：2004-10-25
申请人： Gyula Vigh , Peniel Lim
发明人： Gyula Vigh , Peniel Lim
IPC分类号： B01D61/42
CPC分类号： G01N27/44795 , B01D61/425 , C07K1/28 , G01N27/44773
摘要： An electrophoresis apparatus for measuring, characterizing and/or altering a composition of a sample. The apparatus comprises an anode compartment having an anode and a cathode compartment having a cathode. The anode and cathode are spaced at a distance from one another to define an electric field having a direction along a longitudinal axis, and between the anode and cathode compartments can be at least one separation compartment. Each compartment includes means for adding or removing a solution, a first dimension orthogonal to the direction of the electric field, a second dimension orthogonal to the electric field and the first dimension, and a third dimension parallel to the electric field and orthogonal to the first and second dimensions. A ratio of the first and second dimensions define an aspect ratio, at least one aspect ratio being less than one. An ion-permeable barrier is positioned between each compartment to prevent convective mixing therebetween. At least one of the compartments can be made of an electrically insulating material having a thermal conductivity greater than about 1 W/mK and a specific heat greater than about 100 J/kgK.
摘要翻译：一种用于测量，表征和/或改变样品组成的电泳装置。该装置包括具有阳极和具有阴极的阴极室的阳极室。阳极和阴极彼此间隔开一定距离以限定具有沿着纵向轴线的方向的电场，并且阳极和阴极室之间可以是至少一个分隔室。每个隔室包括用于添加或去除溶液的装置，与电场方向正交的第一维度，与电场和第一维度正交的第二维度，以及平行于电场并与第一维度正交的第三维度和第二维度。第一和第二尺寸的比率定义纵横比，至少一个纵横比小于1。离子渗透屏障位于每个隔室之间，以防止它们之间的对流混合。至少一个隔室可以由具有大于约1W / mK的热导率和大于约100J / kgK的比热的电绝缘材料制成。

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