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1. 发明授权

US11560584B2 Phage-mediated immunoassay and methods for determining susceptibility of bacteria to antibiotic or probiotic agents 有权
公开(公告)号：US11560584B2
公开(公告)日：2023-01-24
申请号：US15860506
申请日：2018-01-02
申请人： QUIDEL CORPORATION , GUILD ASSOCIATES, INC.
发明人： Alexander Solomon Belenky , David A. Schofield
IPC分类号： C12Q1/04 , C12Q1/18 , C12Q1/689 , G01N33/569
摘要： Methods for determining the susceptibility or resistance of bacteria to antibiotic agents are provided. In one embodiment, the methods include culturing the bacteria in the presence or absence or the antimicrobial agent to generate a primary culture which is then cultured in the presence or absence of transforming phages. The recombinant phages are specific to the bacteria and comprise a heterologous marker (e.g., a nucleic acid that is expressible as a detectable product such as an RNA or a protein). The susceptibility or resistance of the bacteria to the antimicrobial agent may be determined by assaying the culture for the presence or absence of the heterologous marker, wherein a reduction in the level or activity of the marker in the culture compared to the level or activity of the marker in a comparative culture indicates that the bacteria is sensitive to the antibiotic agent.

2. 发明授权

US09944920B2 Biodegradable immobilized enzymes and methods of making the same 有权
公开(公告)号：US09944920B2
公开(公告)日：2018-04-17
申请号：US13675659
申请日：2012-11-13
申请人： Guild Associates, Inc.
发明人： Augustine A. DiNovo , Dominic P. DiNovo , David A. Schofield , Matthew F. Smiechowski , Francis H. Verhoff
IPC分类号： C12N11/10 , C12N11/12 , C12N11/02 , C12N11/06
CPC分类号： C12N11/10 , C12N11/02 , C12N11/06 , C12N11/12
摘要： The present application discloses immobilized enzymes and immobilized enzyme materials comprising a crosslinked enzyme having a support material which includes a biomass material different than the biomass used to initially derive the enzyme. Optionally, the immobilized enzyme further includes a polymeric material and/or the biomass which was used to initially derive the enzyme. The resulting immobilized enzyme materials may be biodegradable. The present application also discloses methods of making and using the disclosed immobilized enzyme materials.

3. 发明申请

US20190078072A1 BIODEGRADABLE IMMOBILIZED ENZYMES AND METHODS OF MAKING THE SAME 审中-公开
公开(公告)号：US20190078072A1
公开(公告)日：2019-03-14
申请号：US15917014
申请日：2018-03-09
申请人： Guild Associates, Inc.
发明人： Augustine A. DiNovo , Dominic DiNovo , David A. Schofield , Matthew F. Smiechowski , Francis H. Verhoff
IPC分类号： C12N11/10 , C12N11/02 , C12N11/12 , C12N11/06
摘要： The present application discloses immobilized enzymes and immobilized enzyme materials comprising a crosslinked enzyme having a support material which includes a biomass material different than the biomass used to initially derive the enzyme. Optionally, the immobilized enzyme further includes a polymeric material and/or the biomass which was used to initially derive the enzyme. The resulting immobilized enzyme materials may be biodegradable. The present application also discloses methods of making and using the disclosed immobilized enzyme materials.

4. 发明申请

US20160002710A1 REPORTER PHAGE AND DIAGNOSTIC FOR BACTERIA 审中-公开
标题翻译：用于细菌的报告器噬菌体和诊断
公开(公告)号：US20160002710A1
公开(公告)日：2016-01-07
申请号：US14668194
申请日：2015-03-25
申请人： Guild Associates, Inc.
发明人： David A. Schofield , Darren J. Wray , Ian J. Molineux
IPC分类号： C12Q1/68
CPC分类号： C12Q1/689 , C12Q1/6897 , Y02A50/451
摘要： The present disclosure relates to compositions, methods, systems and kits for the detection of microorganisms of the Shigella species, including S. flexneri, S. dysenteriae, S. sonnei, and S. boydii. The disclosure relates to recombinant phage operable to infect a S. flexneri microorganism, the phage comprising a detectable reporter. Detection systems of the disclosure may comprise a phage operable to infect a S. flexneri microorganism, and may comprise a reporter nucleic acid expressible upon infection of a S. flexneri microorganism by the phage. The system may be operable to detect the expression of the reporter. A detectable reporter may comprise any gene having bioluminescent, colorimetric and/or visual detectability. Live and infectious S. flexneri microbes may be detected by the compositions, methods, systems and kits described herein.
摘要翻译：本公开涉及用于检测志贺氏菌属物种的微生物的组合物，方法，系统和试剂盒，包括灵芝菌，痢疾杆菌，S. sonnei和S. boydii。本公开涉及可操作以感染弯孢杆菌微生物的重组噬菌体，所述噬菌体包含可检测的报道分子。本公开的检测系统可以包含可操作以感染柔毛霉微生物的噬菌体，并且可以包含在噬菌体感染柔毛霉微生物时可表达的报道核酸。该系统可操作以检测报告人的表达。可检测的报告物可以包含具有生物发光，比色和/或视觉可检测性的任何基因。通过本文所述的组合物，方法，系统和试剂盒可以检测活的和感染性的S. flexneri微生物。

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