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1. 发明申请

US20060088834A1 Matrix analysis of gene expression in cells (magec) 审中-公开
标题翻译：细胞中基因表达的基质分析（magec）
公开(公告)号：US20060088834A1
公开(公告)日：2006-04-27
申请号：US10510722
申请日：2003-04-14
申请人： Gretchen Bain , Lorrie Patricia Daggett , Mark Massari , Xin Zhang
发明人： Gretchen Bain , Lorrie Patricia Daggett , Mark Massari , Xin Zhang
IPC分类号： C12Q1/68 , C12N15/87
CPC分类号： G01N33/74 , G01N33/5023 , G01N33/5041 , G01N33/569 , G01N33/6803 , G01N2333/726 , G01N2500/00
摘要： The invention provides a novel expression cloning technique referred to as a matrix analysis of gene expression in cells (MAGEC) that allows for the indexed introduction, and analysis of nucleic acids in a host cell. While normally one takes cells attached to a surface followed by contacting the cells with heterologous DNA under conditions favoring the uptake of the heterologous DNA, the present invention, in sharp contrasts, proposes affixing (depositing) a nucleic acid-containing mixture onto a suitable surface and thereafter contacting suitable host cells (target cells) with the DNA-containing markings under conditions favoring uptake by the cells of the heterologous expression vector comprising a the target nucleic slide acid molecule. The method enables one to further characterize the gene product(s) of a known gene and unknown in a high-throughput assay format. It essentially allows for the identification of a gene based upon the function of its gene product.
摘要翻译：本发明提供了一种新颖的表达克隆技术，被称为能够进行索引引入的细胞中的基因表达（MAGEC）的基因分析和宿主细胞中核酸的分析。尽管通常，在有利于异源DNA摄取的条件下，通常将细胞附着于表面，随后将细胞与异源DNA接触，本发明的鲜明对照提出将含核酸的混合物附着（沉积）到合适的表面上然后在有利于细胞摄取包含靶核酸载体酸分子的异源表达载体的条件下，使合适的宿主细胞（靶细胞）与含有DNA的标记接触。该方法使得能够进一步表征已知基因的基因产物，并且以高通量测定形式未知。它基本上允许基于其基因产物的功能鉴定基因。

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