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1. 发明授权

US07981676B2 Method for transfer of molecular substances with prokaryotic nucleic acid-binding proteins 失效
标题翻译：用原核核酸结合蛋白转移分子物质的方法
公开(公告)号：US07981676B2
公开(公告)日：2011-07-19
申请号：US12207467
申请日：2008-09-09
申请人： Gerald Boehm , Dirk Esser
发明人： Gerald Boehm , Dirk Esser
IPC分类号： C12N15/00
CPC分类号： C07K19/00 , C07K14/005 , C12N15/87 , C12N2710/10022 , C12N2710/16222 , C12N2710/16622 , C12N2740/16022
摘要： The invention relates to a method for the transfer of molecular substances, for example proteins or nucleic acids in cells, in the case of using DNA combined with a possible gene expression. A prokaryotic nucleic acid-binding protein is used for the transfer, which is preferably obtained from a thermostable organism. Where the substance to be transferred is a nucleic acid, the protein forms a reversible complex with the nucleic acid. The prokaryotic protein condenses and compacts the nucleic acids. Said nucleic acids can be taken up in the target cells after suitable incubation.
摘要翻译：本发明涉及在使用结合可能的基因表达的DNA的情况下转移分子物质例如细胞中的蛋白质或核酸的方法。原核的核酸结合蛋白用于转移，其优选从热稳定生物获得。当要转移的物质是核酸时，蛋白质与核酸形成可逆复合物。原核蛋白凝结并压制核酸。所述核酸可以在合适的温育后在靶细胞中吸收。

2. 发明申请

US20050266020A1 Modular transport systems for molecular substances and production and use thereof 审中-公开
标题翻译：分子物质的模块化运输系统及其生产和使用
公开(公告)号：US20050266020A1
公开(公告)日：2005-12-01
申请号：US11120543
申请日：2005-05-02
申请人： Gerald Boehm , Rainer Rudolph , Ulrich Schmidt , Dirk Esser
发明人： Gerald Boehm , Rainer Rudolph , Ulrich Schmidt , Dirk Esser
IPC分类号： C12N15/09 , A61K38/00 , A61K47/48 , A61K48/00 , A61P7/04 , A61P31/18 , A61P35/00 , C07K14/025 , C12N7/01 , C12N7/04 , A61K49/00 , A61K39/12 , C12N7/00
CPC分类号： C07K14/005 , A61K38/00 , A61K47/6901 , A61K48/00 , C12N7/00 , C12N2710/22022 , C12N2710/22023 , C12N2810/50 , Y02A50/467
摘要： The invention relates to transport systems for molecular substances, comprising a mosaic of recombinant partial units (individual components). The invention further relates to production of the molecular transport system and use thereof.
摘要翻译：本发明涉及用于分子物质的运输系统，其包括重组部分单元（单独组分）的镶嵌物。本发明还涉及分子运输系统的生产及其用途。

3. 发明授权

US07091028B1 Method for directed packaging of molecular substances in protein shells 失效
标题翻译：蛋白质壳中分子物质定向包装的方法
公开(公告)号：US07091028B1
公开(公告)日：2006-08-15
申请号：US10129207
申请日：2000-11-03
申请人： Gerald Boehm , Dirk Esser , Ulrich Schmidt
发明人： Gerald Boehm , Dirk Esser , Ulrich Schmidt
IPC分类号： C12N7/02
CPC分类号： A61K9/5184 , A61K47/6901 , A61K48/00 , C07K14/005 , C07K2319/00 , C12N7/00 , C12N15/62 , C12N2710/22022 , C12N2710/22023 , C12N2810/50 , Y02A50/467
摘要： The invention relates to a method for packaging molecular substances in protein shells, comprising the following steps: binding a protein shell fragment, via a first region to a matrix; bringing the protein shell fragment bound to the matrix into contact with the molecular substance, in order to bind the molecular substance to a second region of the protein shell fragment; separating the protein shell fragment with the bonded molecular substance, or the part thereof which contains the bonded molecular substance, from the matrix and assembling the separated protein shell fragments, or the part thereof which contains the bonded molecular substance, with other protein shell fragments to form a protein shell, whereby the separation and assembly can be carried out in any order.
摘要翻译：本发明涉及一种在蛋白质壳中包装分子物质的方法，包括以下步骤：将蛋白质壳片段经由第一区域与基质结合; 将与基质结合的蛋白质壳片段与分子物质接触，以便将分子物质结合到蛋白质壳片段的第二区域; 将含有结合分子物质的结合分子物质或其部分的蛋白质壳片段与基质分离，并将分离的蛋白质壳片段或其包含结合分子物质的部分与其它蛋白质壳片段组装到形成蛋白质壳，由此分离和组装可以以任何顺序进行。

4. 发明申请

US20050170504A1 Method for transfer of molecular substances with prokaryontic nucleic acid-binding proteins 失效
标题翻译：用原核核酸结合蛋白转移分子物质的方法
公开(公告)号：US20050170504A1
公开(公告)日：2005-08-04
申请号：US10954549
申请日：2004-09-29
申请人： Gerald Boehm , Dirk Esser
发明人： Gerald Boehm , Dirk Esser
IPC分类号： C12N15/09 , A61K31/711 , A61K31/715 , A61K38/00 , A61K38/22 , A61K38/43 , A61K48/00 , A61P7/00 , A61P21/04 , A61P31/18 , A61P35/00 , A61P43/00 , C07K14/005 , C07K19/00 , C12N5/10 , C12N15/87 , C12N15/85 , C12N15/74
CPC分类号： C07K19/00 , C07K14/005 , C12N15/87 , C12N2710/10022 , C12N2710/16222 , C12N2710/16622 , C12N2740/16022
摘要： The invention relates to a method for the transfer of molecular substances, for example proteins or nucleic acids in cells, in the case of using DNA combined with a possible gene expression. A prokaryotic nucleic acid-binding protein is used for the transfer, which is preferably obtained from a thermostable organism. Where the substance to be transferred is a nucleic acid, the protein forms a reversible complex with the nucleic acid. The prokaryotic protein condenses and compacts the nucleic acids. Said nucleic acids can be taken up in the target cells after suitable incubation.
摘要翻译：本发明涉及在使用结合可能的基因表达的DNA的情况下转移分子物质例如细胞中的蛋白质或核酸的方法。原核的核酸结合蛋白用于转移，其优选从热稳定生物获得。当要转移的物质是核酸时，蛋白质与核酸形成可逆复合物。原核蛋白凝结并压制核酸。所述核酸可以在合适的温育后在靶细胞中吸收。

5. 发明授权

US07435595B2 Method for transfer of molecular substances with prokaryotic nucleic acid-binding proteins 失效
标题翻译：用原核核酸结合蛋白转移分子物质的方法
公开(公告)号：US07435595B2
公开(公告)日：2008-10-14
申请号：US10954549
申请日：2004-09-29
申请人： Gerald Boehm , Dirk Esser
发明人： Gerald Boehm , Dirk Esser
IPC分类号： C12N15/00 , C12N15/88
CPC分类号： C07K19/00 , C07K14/005 , C12N15/87 , C12N2710/10022 , C12N2710/16222 , C12N2710/16622 , C12N2740/16022
摘要： The invention relates to a method for the transfer of molecular substances, for example proteins or nucleic acids in cells, in the case of using DNA combined with a possible gene expression. A prokaryotic nucleic acid-binding protein is used for the transfer, which is preferably obtained from a thermostable organism. Where the substance to be transferred is a nucleic acid, the protein forms a reversible complex with the nucleic acid. The prokaryotic protein condenses and compacts the nucleic acids. Said nucleic acids can be taken up in the target cells after suitable incubation.
摘要翻译：本发明涉及在使用结合可能的基因表达的DNA的情况下转移分子物质例如细胞中的蛋白质或核酸的方法。原核的核酸结合蛋白用于转移，其优选从热稳定生物获得。当要转移的物质是核酸时，蛋白质与核酸形成可逆复合物。原核蛋白凝结并压制核酸。所述核酸可以在合适的温育后在靶细胞中吸收。

6. 发明申请

US20090155912A1 METHOD FOR TRANSFER OF MOLECULAR SUBSTANCES WITH PROKARYONTIC NUCLEIC ACID-BINDING PROTEINS 失效
标题翻译：用衍生核酸结合蛋白转移分子物质的方法
公开(公告)号：US20090155912A1
公开(公告)日：2009-06-18
申请号：US12207467
申请日：2008-09-09
申请人： Gerald Boehm , Dirk Esser
发明人： Gerald Boehm , Dirk Esser
IPC分类号： C12N15/74
CPC分类号： C07K19/00 , C07K14/005 , C12N15/87 , C12N2710/10022 , C12N2710/16222 , C12N2710/16622 , C12N2740/16022
摘要： The invention relates to a method for the transfer of molecular substances, for example proteins or nucleic acids in cells, in the case of using DNA combined with a possible gene expression. A prokaryotic nucleic acid-binding protein is used for the transfer, which is preferably obtained from a thermostable organism. Where the substance to be transferred is a nucleic acid, the protein forms a reversible complex with the nucleic acid. The prokaryotic protein condenses and compacts the nucleic acids. Said nucleic acids can be taken up in the target cells after suitable incubation.
摘要翻译：本发明涉及在使用结合可能的基因表达的DNA的情况下转移分子物质例如细胞中的蛋白质或核酸的方法。原核的核酸结合蛋白用于转移，其优选从热稳定生物获得。当要转移的物质是核酸时，蛋白质与核酸形成可逆复合物。原核蛋白凝结并压制核酸。所述核酸可以在合适的温育后在靶细胞中吸收。

7. 发明授权

US08455447B2 Modified therapeutic agents 有权
标题翻译：改性治疗剂
公开(公告)号：US08455447B2
公开(公告)日：2013-06-04
申请号：US12688252
申请日：2010-01-15
申请人： Dirk Esser , Jason Richard Betley , Simon Hugh Ridley
发明人： Dirk Esser , Jason Richard Betley , Simon Hugh Ridley
IPC分类号： A61K38/10 , A61K38/16 , A61K38/00 , A61K38/04 , C07K5/00 , C07K7/00 , C07K16/00 , C07K17/00
CPC分类号： C07K7/06 , C07K7/08
摘要： The invention provides a modified therapeutic agent, said modified agent comprising three or more membrane binding elements with low membrane affinity covalently associated with the agent which elements are capable of interacting independently and with thermodynamic additivity, with components of cellular or artificial membranes exposed to extracellular fluids wherein at least two membrane binding elements are lipophilic elements, which may be aliphatic acyl groups, which may be selected from the list consisting of Myristoyl, Decanoyl or Hexanoyl.
摘要翻译：本发明提供了一种修饰的治疗剂，所述改性剂包含三种或更多种具有低膜亲和力的膜结合元件，所述膜结合元件与所述试剂共价结合，该元件能够独立地相互作用并具有热力学加和性，细胞或人造膜的组分暴露于细胞外液其中至少两个膜结合元件是亲脂性元件，其可以是脂族酰基，其可以选自由肉豆蔻酰，癸酰基或己酰基组成的列表。

8. 发明申请

US20060241048A1 Modified therapeutic agents 审中-公开
公开(公告)号：US20060241048A1
公开(公告)日：2006-10-26
申请号：US10526808
申请日：2003-09-05
申请人： Dirk Esser , Jason Betley , Simon Ridley
发明人： Dirk Esser , Jason Betley , Simon Ridley
IPC分类号： A61K38/10 , C07K7/08
CPC分类号： C07K7/06 , C07K7/08
摘要： The invention provides a modified therapeutic agent, said modified agent comprising three or more membrane binding elements with low membrane affinity covalently associated with the agent which elements are capable of interacting independently and with thermodynamic additivity, with components of cellular or artificial membranes exposed to extracellular fluids wherein at least two membrane binding elements are lipophilic elements, which may be aliphatic acyl groups, which may be selected from the list consisting of Myristoyl, Decanoyl or Hexanoyl.

9. 发明申请

US20050090651A1 Polymeric compounds 审中-公开
标题翻译：聚合物
公开(公告)号：US20050090651A1
公开(公告)日：2005-04-28
申请号：US10491216
申请日：2002-09-26
申请人： Richard Smith , Jason Betley , Dirk Esser
发明人： Richard Smith , Jason Betley , Dirk Esser
IPC分类号： C07K1/04 , C07K1/107 , C07K14/705 , C07K14/815 , C07K14/00 , C07D279/12
CPC分类号： C07K14/70596 , C07K1/047 , C07K1/1075 , C07K1/1077 , C07K14/815
摘要： The invention provides a process for forming a compound having the formula (I): from chemical entities native [A], native [B] and native [C], where native [A] has a thioester group, native [B] had a 1-amino-2-thiol group with an unoxidised sulfhydryl side chain, and native [C] had a thiol reactive function (TRF) group, wherein chemical entities [A], [B] and [C] are covalently linked by linker group L, comprising the steps of: (i) admixing native [A] and native [B] in a reaction solution; (ii) condensing the unoxidised sulfydryl side chain of native [B] with the thioester group of native [A] for producing a first intermediate compound wherein [A] and [B] are linked with a β-aminothioester bond; (iii) rearranging the β-aminothioester bond for producing a second intermediate compound wherein [A] and [B] are linked with an amide bond having attached thereto a free thiol group; (iv) admixing native [C] with the second intermediate compound in a reaction solution; and (v) reacting the thiol reactive function (TRF) group of native [C] with the free thiol group of the second intermediate compound for producing a compound having the formula (I).
摘要翻译：本发明提供了一种用于形成具有式（I）的化合物的方法：从天然[A]，天然[B]和天然[C]的化学实体，其中天然[A]具有硫酯基，天然[B]具有具有未氧化巯基侧链的1-氨基-2-硫醇基团，天然[C]具有硫醇反应性功能（TRF）基团，其中化学实体[A]，[B]和[C]通过连接基团共价连接 L，包括以下步骤：（i）将天然[A]和天然[B]混合在反应溶液中; （ii）将天然[B]的未氧化硫辛酸侧链与天然[A]的硫酯基团缩合，以制备其中[A]和[B]与β-氨基硫酯键连接的第一中间体化合物; （iii）重新布置β-氨基硫酯键以制备第二中间体化合物，其中[A]和[B]与附着有游离巯基的酰胺键连接; （iv）将天然[C]与第二中间体化合物在反应溶液中混合; 和（v）使天然[C]的硫醇反应性官能团（TRF）基团与第二中间体化合物的游离巯基反应，以制备具有式（I）的化合物。

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