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1. 发明授权

US5483469A Multiple sort flow cytometer 失效
标题翻译：多重流式细胞仪
公开(公告)号：US5483469A
公开(公告)日：1996-01-09
申请号：US100762
申请日：1993-08-02
申请人： Ger Van den Engh , Richard J. Esposito
发明人： Ger Van den Engh , Richard J. Esposito
IPC分类号： G01N15/14 , B07C5/344
CPC分类号： G01N15/14 , G01N2015/149
摘要： A flow cytometer utilizes multiple lasers for excitation and respective fluorescence of identified dyes bonded to specific cells or events to identify and verify multiple events to be sorted from a sheath flow and droplet stream. Once identified, verified and timed in the sheath flow, each event is independently tagged upon separation from the flow by an electrical charge of +60, +120, or +180 volts and passed through oppositely charged deflection plates with ground planes to yield a focused six way deflection of at least six events in a narrow plane.
摘要翻译：流式细胞仪利用多个激光器激发和识别结合到特定细胞或事件的识别染料的相应荧光，以鉴定和验证从鞘流和液滴流中分选的多个事件。一旦识别，验证并定时在鞘流中，每个事件在+ 60，+120或+180伏的电荷与流动分离时被独立地标记，并通过带有接地平面的带相反电荷的偏转板，以产生聚焦在狭窄的平面中至少六次事件的六路偏转。

2. 发明授权

US5643796A System for sensing droplet formation time delay in a flow cytometer 失效
标题翻译：用于感测流式细胞仪中液滴形成时间延迟的系统
公开(公告)号：US5643796A
公开(公告)日：1997-07-01
申请号：US323358
申请日：1994-10-14
申请人： Ger Van den Engh , Richard J. Esposito
发明人： Ger Van den Engh , Richard J. Esposito
IPC分类号： G01N15/14 , G01N35/00
CPC分类号： G01N15/1404 , G01N2015/149 , Y10T436/11 , Y10T436/115831
摘要： A droplet flow cytometer system which includes a system to optimize the droplet formation time delay based on conditions actually experienced includes an automatic droplet sampler which rapidly moves a plurality of containers stepwise through the droplet stream while simultaneously adjusting the droplet time delay. Through the system sampling of an actual substance to be processed can be used to minimize the effect of the substances variations or the determination of which time delay is optimal. Analysis such as cell counting and the like may be conducted manually or automatically and input to a time delay adjustment which may then act with analysis equipment to revise the time delay estimate actually applied during processing. The automatic sampler can be controlled through a microprocessor and appropriate programming to bracket an initial droplet formation time delay estimate. When maximization counts through volume, weight, or other types of analysis exists in the containers, the increment may then be reduced for a more accurate ultimate setting. This may be accomplished while actually processing the sample without interruption.
摘要翻译：包括基于实际经历的条件来优化液滴形成时间延迟的系统的液滴流式细胞仪系统包括自动液滴采样器，其自动地将多个容器逐步地流过液滴流，同时调节液滴时间延迟。通过对待处理的实际物质的系统采样可以用于最小化物质变化的影响或确定哪个时间延迟是最佳的。可以手动或自动地进行诸如细胞计数等的分析，并输入到时间延迟调整，然后可以与分析设备一起操作以修改在处理期间实际应用的时间延迟估计。自动采样器可以通过微处理器进行控制，并进行适当的编程以支持初始液滴形成时间延迟估计。当最大化通过体积，重量或其他类型的分析计数存在于容器中时，可以减小增量以获得更准确的最终设置。这可以在不间断地实际处理样品的同时完成。

3. 发明授权

US5726364A Sample introduction apparatus for a flow cytometer 失效
标题翻译：用于流式细胞仪的样品引入装置
公开(公告)号：US5726364A
公开(公告)日：1998-03-10
申请号：US798177
申请日：1997-02-10
申请人： Ger Van den Engh
发明人： Ger Van den Engh
IPC分类号： G01N1/00 , G01N15/14 , G01N35/10
CPC分类号： G01N15/1404 , G01N35/1095
摘要： A sample introduction system for a flow cytometer allows easy change of sample containers such as test tubes and facilitates use in high pressure environments. The sample container includes a cap having a pressure supply chamber and a sample container attachment cavity. A sample container may be automatically positioned into the attachment cavity so as to sealably engage the end of the sample container as its outer surface. This positioning may be accomplished through some sample introduction mechanism. To facilitate cleaning HPLC tubing and fittings may be used in a manner which facilitates removable of the entire tubing from both the nozzle container and other sample container cap to permit its replacement to avoid contamination. The sample container support may include horizontal stops which loosely limit the movement of the sample container and thus avoid further stresses upon it.
摘要翻译：用于流式细胞仪的样品引入系统允许容易地更换样品容器，例如试管，并且便于在高压环境中使用。样品容器包括具有压力供应室和样品容器附着腔的盖。样品容器可以自动地定位到附件空腔中，以便与其外表面密封地接合样品容器的端部。这种定位可以通过一些样品引入机制来实现。为了方便清洗，HPLC管道和接头可以以便于从喷嘴容器和其他样品容器盖两者移除整个管道的方式使用，以允许其更换以避免污染。样品容器支撑件可以包括水平止挡件，其松散地限制了样品容器的移动，并因此避免了其上的进一步的压力。

4. 发明授权

US07923252B2 Droplet formation systems for flow cytometers 有权
标题翻译：流式细胞仪的液滴形成系统
公开(公告)号：US07923252B2
公开(公告)日：2011-04-12
申请号：US11069529
申请日：2005-02-28
申请人： Ger Van den Engh
发明人： Ger Van den Engh
IPC分类号： G01N21/85 , G01N33/48
CPC分类号： G01N15/1404 , G01N2015/1406 , G01N2015/1409 , Y10T436/25375 , Y10T436/2575
摘要： A droplet forming flow cytometer system (1) allows high speed processing without the need for high oscillator drive powers through the inclusion of an oscillator or piezoelectric crystal (10) within the nozzle volume (3) and directly coupled to the sheath fluid. The nozzle container (27) continuously converges so as to amplify unidirectional oscillations (11) which are transmitted as pressure waves through the nozzle volume (3) to the nozzle exit so as to form droplets from the fluid jet. A variation in substance concentration is achieved through a movable substance introduction port (9) which is positioned within a convergence zone (32) to vary the relative concentration of substance to sheath fluid while still maintaining optimal laminar flow conditions. This variation may be automatically controlled through a sensor and controller configuration.
摘要翻译：液滴形成流式细胞仪系统（1）允许高速处理，而不需要通过在喷嘴体积（3）内包含振荡器或压电晶体（10）并且直接耦合到鞘液的高振荡器驱动功率。喷嘴容器（27）连续会聚，以便放大单向振荡（11），其作为压力波通过喷嘴容积（3）传递到喷嘴出口，以便从流体射流形成液滴。通过位于会聚区（32）内的可移动物质引入口（9）来实现物质浓度的变化，以改变物质与鞘液的相对浓度，同时仍保持最佳的层流条件。这种变化可以通过传感器和控制器配置自动控制。

5. 发明授权

US5602039A Flow cytometer jet monitor system 失效
标题翻译：流式细胞仪喷射监测系统
公开(公告)号：US5602039A
公开(公告)日：1997-02-11
申请号：US323352
申请日：1994-10-14
申请人： Ger Van den Engh
发明人： Ger Van den Engh
IPC分类号： G01N15/10 , G01N15/14
CPC分类号： G01N15/1404 , G01N2015/1406 , G01N2015/149
摘要： A direct jet monitor illuminates the jet of a flow cytometer in a monitor wavelength band which is substantially separate from the substance wavelength band. When a laser is used to cause fluorescence of the substance, it may be appropriate to use an infrared source to illuminate the jet and thus optically monitor the conditions within the jet through a CCD camera or the like. This optical monitoring may be provided to some type of controller or feedback system which automatically changes either the horizontal location of the jet, the point at which droplet separation occurs, or some other condition within the jet in order to maintain optimum conditions. The direct jet monitor may be operated simultaneously with the substance property sensing and analysis system so that continuous monitoring may be achieved without interfering with the substance data gathering and may be configured so as to allow the front of the analysis or free fall area to be unobstructed during processing.
摘要翻译：直接喷射监测器照射与物质波长带基本分离的监测波长带中的流式细胞仪的射流。当使用激光来引起物质的荧光时，使用红外光源来照射射流并因此通过CCD照相机等光学地监测射流内的状况可能是合适的。该光学监视可以被提供给某种类型的控制器或反馈系统，其自动地改变射流的水平位置，发生液滴分离的点，或喷射内的某些其他状态，以便保持最佳条件。直接喷射监测器可以与物质性质感测和分析系统同时操作，使得可以在不干扰物质数据收集的情况下实现连续监测，并且可以将其配置成允许分析或自由落体区域的前部不受阻碍在处理过程中。

6. 发明授权

US6133044A High speed flow cytometer droplet formation system and method 失效
标题翻译：高速流式细胞仪液滴形成系统及方法
公开(公告)号：US6133044A
公开(公告)日：2000-10-17
申请号：US627963
申请日：1996-04-16
申请人： Ger Van den Engh
发明人： Ger Van den Engh
IPC分类号： G01N15/14 , G01N1/18
CPC分类号： G01N15/1404 , G01N2015/1406 , G01N2015/1409 , Y10T436/117497 , Y10T436/118339 , Y10T436/25375 , Y10T436/2575
摘要： A droplet forming flow cytometer system allows high speed processing without the need for high oscillator drive powers through the inclusion of an oscillator or piezoelectric crystal such as within the nozzle volume or otherwise unidirectionally coupled to the sheath fluid. The nozzle container continuously converges so as to amplify unidirectional oscillations which are transmitted as pressure waves through the nozzle volume to the nozzle exit so as to form droplets from the fluid jet. The oscillator is directionally isolated so as to avoid moving the entire nozzle container so as to create only pressure waves within the sheath fluid. A variation in substance concentration is achieved through a movable substance introduction port which is positioned within a convergence zone to vary the relative concentration of substance to sheath fluid while still maintaining optimal laminar flow conditions. This variation may be automatically controlled through a sensor and controller configuration. A replaceable tip design is also provided whereby the ceramic nozzle tip is positioned within an edge insert in the nozzle body so as to smoothly transition from nozzle body to nozzle tip. The nozzle tip is sealed against its outer surface to the nozzle body so it may be removable for cleaning or replacement.
摘要翻译：液滴形成流式细胞仪系统允许高速处理，而不需要通过包括振荡器或压电晶体（例如在喷嘴体积内）或以其它方式单独耦合到鞘液的高振荡器驱动功率。喷嘴容器连续会聚，以便放大单向振荡，其作为压力波通过喷嘴体积传递到喷嘴出口，以便从流体射流形成液滴。振荡器是定向隔离的，以避免移动整个喷嘴容器，从而仅在护套液体内产生压力波。通过位于会聚区内的可移动物质引入口来实现物质浓度的变化，以改变物质与鞘液的相对浓度，同时仍保持最佳的层流条件。这种变化可以通过传感器和控制器配置自动控制。还提供了可替换的尖端设计，其中陶瓷喷嘴尖端位于喷嘴体内的边缘插入件中，以便从喷嘴体平滑地过渡到喷嘴尖端。喷嘴尖端与其外表面密封到喷嘴体，因此其可以是可移除的用于清洁或更换。

7. 发明申请

US20050153458A1 Droplet formation systems for flow cytometers 有权
标题翻译：流式细胞仪的液滴形成系统
公开(公告)号：US20050153458A1
公开(公告)日：2005-07-14
申请号：US11069529
申请日：2005-02-28
申请人： Ger Van den Engh
发明人： Ger Van den Engh
IPC分类号： G01N15/14 , G01N33/48 , G01N1/10
CPC分类号： G01N15/1404 , G01N2015/1406 , G01N2015/1409 , Y10T436/25375 , Y10T436/2575
摘要： A droplet forming flow cytometer system (1) allows high speed processing without the need for high oscillator drive powers through the inclusion of an oscillator or piezoelectric crystal (10) within the nozzle volume (3) and directly coupled to the sheath fluid. The nozzle container (27) continuously converges so as to amplify unidirectional oscillations (11) which are transmitted as pressure waves through the nozzle volume (3) to the nozzle exit so as to form droplets from the fluid jet. A variation in substance concentration is achieved through a movable substance introduction port (9) which is positioned within a convergence zone (32) to vary the relative concentration of substance to sheath fluid while still maintaining optimal laminar flow conditions. This variation may be automatically controlled through a sensor and controller configuration.
摘要翻译：液滴形成流式细胞仪系统（1）允许高速处理，而不需要在喷嘴体积（3）内包括振荡器或压电晶体（10）并直接耦合到鞘液的高振荡器驱动功率。喷嘴容器（27）连续会聚，以便放大单向振荡（11），其作为压力波通过喷嘴容积（3）传递到喷嘴出口，以便从流体射流形成液滴。通过位于会聚区（32）内的可移动物质引入口（9）来实现物质浓度的变化，以改变物质与鞘液的相对浓度，同时仍保持最佳的层流条件。这种变化可以通过传感器和控制器配置自动控制。

8. 发明授权

US06861265B1 Flow cytometer droplet formation system 失效
标题翻译：流式细胞仪液滴形成系统
公开(公告)号：US06861265B1
公开(公告)日：2005-03-01
申请号：US09689585
申请日：2000-10-12
申请人： Ger Van den Engh
发明人： Ger Van den Engh
IPC分类号： G01N15/14 , G01N15/10
CPC分类号： G01N15/1404 , G01N2015/1406 , G01N2015/1409 , Y10T436/25375 , Y10T436/2575
摘要： A droplet forming flow cytometer system allows high speed processing without the need for high oscillator drive powers through the inclusion of an oscillator or piezoelectric crystal within the nozzle volume and directly coupled to the sheath fluid. The nozzle contain converges so as to amplify unidirectional oscillations which are transmitted as pressure waves through the nozzle volume to the nozzle exit so as to form droplets from the fluid jet. The oscillator is directionally isolated so as to avoid moving the entire nozzle container so as to create only pressure waves within the sheath fluid. A variation in substance concentration is achieved through a movable substance introduction port which is positioned within a convergence zone to vary the relative concentration of substance to sheath fluid while still maintaining optimal laminar flow conditions. This variation may be automatically controlled through a sensor and controller configuration. A replaceable tip design is also provided whereby the ceramic nozzle tip is positioned within an edge insert in the mozzle body so as to smoothly transition from nozzle body to nozzle tip. The nozzle tip is sealed against its outer surface to the nozzle body so it may be removable for cleaning or replacement.
摘要翻译：液滴形成流式细胞仪系统允许高速处理，而不需要在喷嘴体积内包括振荡器或压电晶体并且直接耦合到鞘液的高振荡器驱动功率。喷嘴包含会聚，以便放大单向振荡，其作为压力波通过喷嘴体积传送到喷嘴出口，以便从流体射流形成液滴。振荡器是定向隔离的，以避免移动整个喷嘴容器，从而仅在护套液体内产生压力波。通过位于会聚区内的可移动物质引入口来实现物质浓度的变化，以改变物质与鞘液的相对浓度，同时仍保持最佳的层流条件。这种变化可以通过传感器和控制器配置自动控制。还提供了可替换的尖端设计，其中陶瓷喷嘴尖端位于溜槽主体中的边缘插入件内，以便从喷嘴体平滑地过渡到喷嘴尖端。喷嘴尖端与其外表面密封到喷嘴体，因此其可以是可移除的用于清洁或更换。

9. 发明授权

US5602349A Sample introduction system for a flow cytometer 失效
标题翻译：流式细胞仪样品引入系统
公开(公告)号：US5602349A
公开(公告)日：1997-02-11
申请号：US323348
申请日：1994-10-14
申请人： Ger Van den Engh
发明人： Ger Van den Engh
IPC分类号： G01N1/00 , G01N15/14 , G01N35/10
CPC分类号： G01N15/1404 , G01N35/1095
摘要： A sample introduction system for a flow cytometer allows easy change of sample containers such as test tubes and facilitates use in high pressure environments. The sample container includes a cap having a pressure supply chamber and a sample container attachment cavity. A sample container may be automatically positioned into the attachment cavity so as to sealably engage the end of the sample container as its outer surface. This positioning may be accomplished through some sample introduction mechanism. To facilitate cleaning, HPLC tubing and fittings may be used in a manner which facilitates removing of the entire tubing from both the nozzle container and other sample container cap to permit its replacement to avoid contamination. The sample container support may include horizontal stops which loosely limit the movement of the sample container and thus avoid further stresses upon it.
摘要翻译：用于流式细胞仪的样品引入系统允许容易地更换样品容器，例如试管，并且便于在高压环境中使用。样品容器包括具有压力供应室和样品容器附着腔的盖。样品容器可以自动地定位到附件空腔中，以便与其外表面密封地接合样品容器的端部。这种定位可以通过一些样品引入机制来实现。为了便于清洁，HPLC管道和配件可以以便于从喷嘴容器和其他样品容器盖两者去除整个管道以允许其更换以避免污染的方式使用。样品容器支撑件可以包括水平止挡件，其松散地限制了样品容器的移动，并因此避免了其上的进一步的压力。

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