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1. 发明授权

US4994166A Single apparatus for slab gel electrophoresis and blotting 失效
标题翻译：板式凝胶电泳和印迹的单一设备
公开(公告)号：US4994166A
公开(公告)日：1991-02-19
申请号：US397065
申请日：1989-08-22
申请人： George G. Fernwood , Efrem G. Hernandez , Frank R. Witney
发明人： George G. Fernwood , Efrem G. Hernandez , Frank R. Witney
IPC分类号： G01N27/447
CPC分类号： G01N27/4473
摘要： Electrophoretic separation in a slab gel and blotting of the resulting zone pattern onto a membrane are both performed in a single tank cell which contains separation electrodes along opposing vertical walls and blotting electrodes horizontally arranged above and below the level designated for the gel placement. The blotting electrode situated below the gel is an array of parallel wires arranged to simulate a plate electrode. The cell is operated in separatory and blotting modes, in which the separation and blotting electrodes, respectively, are separately energized. During the separatory mode, the wires in the array forming the lower blotting electrode are electrically isolated from each other to permit each to adjust to the voltage of the buffer immediately surrounding it.
摘要翻译：板状凝胶中的电泳分离和所得到的区域图案到膜上的印迹都在单个槽室中进行，所述单个槽室包含沿着相对的垂直壁的分离电极和水平地布置在凝胶放置指定的水平之上和之下的吸印电极。位于凝胶下面的印迹电极是布置成模拟平板电极的平行线阵列。电池以分离和印迹模式操作，其中分离和吸印电极分别被分开通电。在分离模式期间，形成下部印迹电极的阵列中的导线彼此电隔离，以允许每个电极根据其周围的缓冲器的电压进行调节。

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