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1. 发明申请

US20080032386A1 Stabilized Proline Transporter 审中-公开
标题翻译：稳定的脯氨酸转运蛋白
公开(公告)号：US20080032386A1
公开(公告)日：2008-02-07
申请号：US11629683
申请日：2005-06-17
申请人： Fumihiko Omura , Nobuyuki Fukui , Hiroto Kondo
发明人： Fumihiko Omura , Nobuyuki Fukui , Hiroto Kondo
IPC分类号： C07H21/04 , C07K16/00 , C12C11/00 , C12N1/00 , C12N15/00
CPC分类号： C07K14/395 , C12C12/004 , C12C12/006
摘要： The present invention relates to a stabilized proline transporter Put4 obtained by gene mutation and a gene encoding the same, as well as a Saccharomyces yeast strain which is obtained by yeast transformation with the gene and is capable of efficiently using proline in a source material such as wort. The stabilized mutated proline transporter Put4 can be used to achieve efficient use of nitrogen sources such as poorly assimilable proline contained in source materials (e.g., wort) for alcohol beverages. Fermentation using yeast capable of taking up a wide variety and large amounts of nitrogen sources facilitates carbon source assimilation and allows improvement of productivity for alcohol beverages. Moreover, the use of poorly assimilable nitrogen sources leads to resource savings and enables environmentally friendly production of alcohol beverages.
摘要翻译：本发明涉及通过基因突变获得的稳定的脯氨酸转运蛋白Put4和编码该基因突变的基因，以及通过用该基因进行酵母转化而获得的酵母菌酵母菌株，并且能够有效地使用源材料中的脯氨酸麦芽汁稳定的突变脯氨酸转运蛋白Put4可用于实现氮源的有效利用，例如酒精饮料的原料（例如麦芽汁）中所含的不良可同化的脯氨酸。使用能够吸收多种和大量氮源的酵母的发酵有助于碳源同化，并且可以提高酒精饮料的生产率。此外，使用不良的可吸收的氮源导致资源节约并且能够环保地生产酒精饮料。

2. 发明申请

US20090110769A1 Process for Producing Fermented Beverages Using Fermentation Raw Material Solutions of Low Maltotriose Ratio 审中-公开
标题翻译：使用低麦芽三糖比的发酵原料生产发酵饮料的方法
公开(公告)号：US20090110769A1
公开(公告)日：2009-04-30
申请号：US11791758
申请日：2005-11-28
申请人： Atsushi Fujita , Hiroto Kondo , Nobuyuki Fukui , Yuji Nishida
发明人： Atsushi Fujita , Hiroto Kondo , Nobuyuki Fukui , Yuji Nishida
IPC分类号： C12C11/00
CPC分类号： C12C5/00
摘要： It is an object of the present invention to provide fermented beverages that give no sweetness in aftertaste and which have less off-flavors by a technique that will not affect the principal taste and aroma characteristics and which is highly acceptable to consumers.To attain this object, the proportion of trisaccharides in the assimilable sugars in the fermentation raw material solution or fermenting solution is adjusted, such that the yet-to-be assimilated sugars which remain in the fermenting step will not consist mainly of trisaccharides. Specifically, fermentation is performed while the proportion of trisaccharides in assimilable sugars in the fermentation raw material solution is adjusted to be no more than 25 wt %.
摘要翻译：本发明的目的是提供不会在后味中产生甜味的发酵饮料，并且通过不会影响主要味道和香气特性并且是消费者高度可接受的技术具有较少的异味。为了达到上述目的，调整发酵原料溶液或发酵液中的可同化糖中的三糖的比例，使得保留在发酵工序中的未被同化的糖不主要由三糖组成。具体而言，发酵原料溶液中的可同化糖中的三糖的比例调整为25重量％以下进行发酵。

3. 发明授权

US5965444A Method for constructing transformant not having selective marker gene 失效
标题翻译：构建不具有选择性标记基因的转化体的方法
公开(公告)号：US5965444A
公开(公告)日：1999-10-12
申请号：US880745
申请日：1997-06-23
申请人： Toshihiko Ashikari , Hiroto Kondo , Keiko Sakakibara , Hiroyuki Araki , Yasuji Oshima
发明人： Toshihiko Ashikari , Hiroto Kondo , Keiko Sakakibara , Hiroyuki Araki , Yasuji Oshima
IPC分类号： C12N9/34 , C12N15/65 , C12N15/81 , C12N15/90 , C12Q1/68 , C07H21/04
CPC分类号： C12N9/2428 , C12N15/65 , C12N15/81 , C12N15/905
摘要： A DNA construct wherein a DNA fragment which is recombinable in yeast chromosomal DNA is directly or indirectly linked at both ends of a DNA fragment which comprises a pair of R sensitive sequences oriented in the same direction and flaking both an R gene placed under the control of an inducible promoter and an expressible selective marker gene, which is a DNA construct designed with the R sensitive sequences non-symmetrically shortened, so that no functionable R sensitive sequence remains after the R sensitive sequence recombination has occurred by expression of the R gene and the selective marker has been removed. Since no functionable R sensitive sequence remains after removal of the selective marker, recombination does not occur again, and thus the same selective marker may be used for multiple insertions of foreign genes.
摘要翻译：一种DNA构建体，其中在酵母染色体DNA中可重组的DNA片段在DNA片段的两端直接或间接连接，所述DNA片段包含一对沿相同方向取向的R敏感序列，可诱导的启动子和可表达的选择性标记基因，其是用R敏感序列非对称缩短设计的DNA构建体，使得在通过R基因的表达发生R敏感序列重组后，不存在可操作的R敏感序列选择性标记已被删除。由于在去除选择性标记物之后不存在可操作的R敏感序列，因此重新不再次发生，因此相同的选择性标记可用于多次插入外源基因。

4. 发明授权

US5750372A Vector having promoter that is inducible by methanol and/or glycerol 失效
标题翻译：具有可被甲醇和/或甘油诱导的启动子的载体
公开(公告)号：US5750372A
公开(公告)日：1998-05-12
申请号：US485284
申请日：1995-06-07
申请人： Yasuyoshi Sakai , Yoshiki Tani , Yuji Shibano , Hiroto Kondo , Haruyo Hatanaka
发明人： Yasuyoshi Sakai , Yoshiki Tani , Yuji Shibano , Hiroto Kondo , Haruyo Hatanaka
IPC分类号： C12N1/19 , C12N9/00 , C12N9/02 , C12N9/04 , C12N9/08 , C12N9/12 , C12N15/09 , C12N15/11 , C12N15/53 , C12N15/54 , C12N15/81 , C12P21/00 , C12R1/01 , C12R1/72 , C12R1/865 , C12P21/06
CPC分类号： C12N9/0065 , C12N15/815 , C12N9/0006 , C12N9/12 , C12Y101/03013
摘要： The present invention relates to an expression vector which is induced by methanol and/or glycerol utilizing an alcohol oxidase gene of a methylotrophic yeast; a recombinant methylotrophic yeast containing said vector and capable of accumulating a significantly high amount of an expression product of a heterologous gene; and a method for producing useful products utilizing said recombinant. The expression vector is constructed by utilizing the promoter and terminator of an alcohol oxidase gene of methylotrophic yeast. Further, a significantly high amount of adenylate kinase, cytochrome C552 or peroxidase is produced by using said expression vector.
摘要翻译：本发明涉及利用甲基营养酵母的醇氧化酶基因由甲醇和/或甘油诱导的表达载体; 含有所述载体的重组甲基营养酵母，能够积累显着高含量的异源基因的表达产物; 以及利用所述重组体生产有用产物的方法。通过利用甲基营养酵母的醇氧化酶基因的启动子和终止子构建表达载体。此外，通过使用所述表达载体产生显着高量的腺苷酸激酶，细胞色素C552或过氧化物酶。

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