专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

1. 发明授权

US07442768B2 Fluorescent protein and gene encoding the same 有权
标题翻译：荧光蛋白和编码基因的基因
公开(公告)号：US07442768B2
公开(公告)日：2008-10-28
申请号：US10953050
申请日：2004-09-30
申请人： Frederick I. Tsuji , Hiroshi Mizuno , Kenji Takase , Mitsuru Momma , Zui Fujimoto , Toshiyuki Wako , Yasuhiro Takenaka , Noboru Nakura , Hiromi Takenaka
发明人： Frederick I. Tsuji , Hiroshi Mizuno , Kenji Takase , Mitsuru Momma , Zui Fujimoto , Toshiyuki Wako , Yasuhiro Takenaka , Noboru Nakura , Hiromi Takenaka
IPC分类号： C12N9/00 , C12P21/06 , C07K1/00
CPC分类号： C07K14/43595 , C07K14/43509
摘要： The present invention provides a novel fluorescent protein the wavelength of the maximum of the fluorescence of which exists in a wavelength side longer than 510 nm, and which exhibits yellow fluorescence or yellowish green fluorescence and can be expressed in a heterogeneous cell, and a gene encoding the same, wherein the fluorescent protein has an amino acid sequence as set forth in SEQ ID NO:1and it is a fluorescent protein derived from a copepod taxonomically classified to Chiridius Poppei.
摘要翻译：本发明提供一种新的荧光蛋白，其荧光最大的波长存在于长于510nm的波长侧，并且呈现黄色荧光或黄绿色荧光，并且可以在异质细胞中表达，并且编码相同，其中荧光蛋白具有如SEQ ID NO：1所示的氨基酸序列，并且其是衍生自分类学分类为Chiridius Poppei的桡足类的荧光蛋白。

2. 发明授权

US08043850B2 Fluorescent protein and gene encoding the same 有权
标题翻译：荧光蛋白和编码基因的基因
公开(公告)号：US08043850B2
公开(公告)日：2011-10-25
申请号：US12204557
申请日：2008-09-04
申请人： Frederick I. Tsuji , Hiroshi Mizuno , Kenji Takase , Mitsuru Momma , Zui Fujimoto , Toshiyuki Wako , Yasuhiro Takenaka , Noboru Nakura , Hiromi Takenaka
发明人： Frederick I. Tsuji , Hiroshi Mizuno , Kenji Takase , Mitsuru Momma , Zui Fujimoto , Toshiyuki Wako , Yasuhiro Takenaka , Noboru Nakura , Hiromi Takenaka
IPC分类号： C12N15/00 , C12Q1/68 , C12P21/06
CPC分类号： C07K14/43595 , C07K14/43509
摘要： The present invention provides a novel fluorescent protein the wavelength of the maximum of the fluorescence of which exists in a wavelength side longer than 510 nm, and which exhibits yellow fluorescence or yellowish green fluorescence and can be expressed in a heterogeneous cell, and a gene encoding the same, wherein the fluorescent protein has an amino acid sequence as set forth in SEQ ID NO: 1 and it is a fluorescent protein derived from a copepod taxonomically classified to Chiridius Poppei.
摘要翻译：本发明提供一种新的荧光蛋白，其荧光最大的波长存在于长于510nm的波长侧，并且呈现黄色荧光或黄绿色荧光，并且可以在异质细胞中表达，并且编码相同，其中荧光蛋白具有如SEQ ID NO：1所示的氨基酸序列，并且其是衍生自分类学分类为Chiridius Poppei的桡足类的荧光蛋白。

3. 发明申请

US20090123969A1 NOVEL FLUORESCENT PROTEIN AND GENE ENCODING THE SAME 有权
标题翻译：新的荧光蛋白和编码它的基因
公开(公告)号：US20090123969A1
公开(公告)日：2009-05-14
申请号：US12204557
申请日：2008-09-04
申请人： Frederick I. Tsuji , Hiroshi Mizuno , Kenji Takase , Mitsuru Momma , Zui Fujimoto , Toshiyuki Wako , Yasuhiro Takenaka , Noboru Nakura , Hiromi Takenaka
发明人： Frederick I. Tsuji , Hiroshi Mizuno , Kenji Takase , Mitsuru Momma , Zui Fujimoto , Toshiyuki Wako , Yasuhiro Takenaka , Noboru Nakura , Hiromi Takenaka
IPC分类号： C12P21/04 , C12N15/11 , C12N15/00
CPC分类号： C07K14/43595 , C07K14/43509
摘要： The present invention provides a novel fluorescent protein the wavelength of the maximum of the fluorescence of which exists in a wavelength side longer than 510 nm, and which exhibits yellow fluorescence or yellowish green fluorescence and can be expressed in a heterogeneous cell, and a gene encoding the same, wherein the fluorescent protein has an amino acid sequence as set forth in SEQ ID NO: 1 and it is a fluorescent protein derived from a copepod taxonomically classified to Chiridius Poppei.
摘要翻译：本发明提供一种新的荧光蛋白，其荧光最大的波长存在于长于510nm的波长侧，并且呈现黄色荧光或黄绿色荧光，并且可以在异质细胞中表达，并且编码相同，其中荧光蛋白具有如SEQ ID NO：1所示的氨基酸序列，并且其是衍生自分类学分类为Chiridius Poppei的桡足类的荧光蛋白。

4. 发明申请

US20050221338A1 Novel fluorescent protein and gene encoding the same 有权
标题翻译：新型荧光蛋白和编码基因的基因
公开(公告)号：US20050221338A1
公开(公告)日：2005-10-06
申请号：US10953050
申请日：2004-09-30
申请人： Frederick Tsuji , Hiroshi Mizuno , Kenji Takase , Mitsuru Momma , Zui Fujimoto , Toshiyuki Wako , Yasuhiro Takenaka , Noboru Nakura , Hiromi Takenaka
发明人： Frederick Tsuji , Hiroshi Mizuno , Kenji Takase , Mitsuru Momma , Zui Fujimoto , Toshiyuki Wako , Yasuhiro Takenaka , Noboru Nakura , Hiromi Takenaka
IPC分类号： C07H21/04 , C07K14/435 , C12N5/06 , C12N15/09 , C12N15/12 , C12Q1/68
CPC分类号： C07K14/43595 , C07K14/43509
摘要： The present invention provides a novel fluorescent protein the wavelength of the maximum of the fluorescence of which exists in a wavelength side longer than 510 nm, and which exhibits yellow fluorescence or yellowish green fluorescence and can be expressed in a heterogeneous cell, and a gene encoding the same, wherein the fluorescent protein has an amino acid sequence: MTTFKIESRI HGNLNGEKFE LVGGGVGEEG RLEIEMKTKD KPLAFSPFLL SHCMGYGFYH 60 FASFPKGTKN IYLHAATNGG YTNTRKEIYE DGGILEVNFR YTYEFNKIIG DVECIGHGFP 120 SQSPIFKDTI VKSCPTVDLM LPMSGNIIAS SYARAFQLKD GSFYTAEVKN NIDFKNPIHE 180 SFSKSGPMFT HRRVEETHTK ENLAMVEYQQ VFNSAPRDM 219 and it is a fluorescent protein derived from a copepod taxonomically classified to Chiridius Poppei.
摘要翻译：本发明提供一种新的荧光蛋白，其荧光最大的波长存在于长于510nm的波长侧，并且呈现黄色荧光或黄绿色荧光，并且可以在异质细胞中表达，并且编码相同的，其中所述荧光蛋白具有氨基酸序列：MTTFKIESRI HGNLNGEKFE LVGGGVGEEG RLEIEMKTKD KPLAFSPFLL SHCMGYGFYH 60 FASFPKGTKN IYLHAATNGG YTNTRKEIYE DGGILEVNFR YTYEFNKIIG DVECIGHGFP 120 SQSPIFKDTI VKSCPTVDLM LPMSGNIIAS SYARAFQLKD GSFYTAEVKN NIDFKNPIHE 180 SFSKSGPMFT HRRVEETHTK ENLAMVEYQQ VFNSAPRDM 219，它是从一个桡足类衍生分类学上的荧光蛋白分类为Chiridius Poppei。

5. 发明授权

US08278120B2 Method of changing fluorescence wavelength of fluorescent protein 有权
标题翻译：改变荧光蛋白荧光波长的方法
公开(公告)号：US08278120B2
公开(公告)日：2012-10-02
申请号：US12162460
申请日：2007-01-25
申请人： Kyoko Suto , Hiromi Takenaka , Yasuhiro Takenaka
发明人： Kyoko Suto , Hiromi Takenaka , Yasuhiro Takenaka
IPC分类号： G01N33/536 , G01N33/533 , C07K1/00 , C07K14/00 , C07K17/00
CPC分类号： C07K14/43595
摘要： The present invention provides: a method of changing the fluorescence wavelength of a GFP-like fluorescent protein from copepod while maintaining recombinant expression efficiency, which comprises identifying a structural factor for determining the fluorescence wavelength thereof in the three-dimensional structure of the protein and modifying amino acid residues associated with the structural factor; and a modified fluorescent protein obtained by applying said method. For example, with regard to a GFP-like fluorescent protein from Chiridius poppei, His52 in an α helix-like secondary structure: PFLLSHCMGYGFYHF (α1 47-61) comprising a fluorescent moiety site GYG is replaced with an aromatic amino acid selected from Phe, Tyr and Trp, so as to cause a red shift of the fluorescent peak wavelength; or it is replaced with Ala, Val, Ile, Leu, Gly, Cys, Met, Ser, Thr, or Asp, Asn, Glu or Gln, so as to cause a blue shift of the fluorescence peak wavelength.
摘要翻译：本发明提供了一种在保持重组表达效率的同时维持来自桡足类的GFP样荧光蛋白的荧光波长的方法，其包括鉴定用于测定蛋白质三维结构中的荧光波长的结构因子和修饰与结构因子相关的氨基酸残基; 和通过应用所述方法获得的修饰的荧光蛋白。例如，关于来自Chiridius poppei的GFP样荧光蛋白，包含荧光部位部位GYG的α螺旋状二级结构的His52被替换为选自Phe的芳香族氨基酸， Tyr和Trp，以引起荧光峰值波长的红移; 或者被Ala，Val，Ile，Leu，Gly，Cys，Met，Ser，Thr或Asp，Asn，Glu或Gln替代，以使荧光峰值波长发生蓝移。

6. 发明申请

US20090318673A1 METHOD OF CHANGING FLUORESCENCE WAVELENGTH OF FLUORESCENT PROTEIN 有权
标题翻译：荧光蛋白荧光波长变化的方法
公开(公告)号：US20090318673A1
公开(公告)日：2009-12-24
申请号：US12162460
申请日：2007-01-25
申请人： Kyoko Suto , Hiromi Takenaka , Yasuhiro Takenaka
发明人： Kyoko Suto , Hiromi Takenaka , Yasuhiro Takenaka
IPC分类号： C07K1/107 , C07K14/435
CPC分类号： C07K14/43595
摘要： The present invention provides: a method of changing the fluorescence wavelength of a GFP-like fluorescent protein from copepod while maintaining recombinant expression efficiency, which comprises identifying a structural factor for determining the fluorescence wavelength thereof in the three-dimensional structure of the protein and modifying amino acid residues associated with the structural factor; and a modified fluorescent protein obtained by applying said method. For example, with regard to a GFP-like fluorescent protein from Chiridius poppei, His52 in an α helix-like secondary structure: PFLLSHCMGYGFYHF (α1 47-61) comprising a fluorescent moiety site GYG is replaced with an aromatic amino acid selected from Phe, Tyr and Trp, so as to cause a red shift of the fluorescent peak wavelength; or it is replaced with Ala, Val, Ile, Leu, Gly, Cys, Met, Ser, Thr, or Asp, Asn, Glu or Gln, so as to cause a blue shift of the fluorescence peak wavelength.
摘要翻译：本发明提供了一种在保持重组表达效率的同时维持来自桡足类的GFP样荧光蛋白的荧光波长的方法，其包括鉴定用于测定蛋白质三维结构中的荧光波长的结构因子和修饰与结构因子相关的氨基酸残基; 和通过应用所述方法获得的修饰的荧光蛋白。例如，关于来自Chiridius poppei的GFP样荧光蛋白，包含荧光部位部位GYG的α螺旋状二级结构的His52被替换为选自以下的芳香族氨基酸：Phe， Tyr和Trp，以引起荧光峰值波长的红移; 或者被Ala，Val，Ile，Leu，Gly，Cys，Met，Ser，Thr或Asp，Asn，Glu或Gln替代，以使荧光峰值波长发生蓝移。

7. 发明申请

US20140008009A1 MOLD, MOLDING JIG AND MOLDING METHOD 有权
标题翻译：模具，模具和模具方法
公开(公告)号：US20140008009A1
公开(公告)日：2014-01-09
申请号：US13976732
申请日：2011-12-26
申请人： Toshikazu Sana , Akihito Sakai , Akiro Murai , Yasuhiro Takenaka , Kazuyoshi Kawano , Tomoya Takahashi
发明人： Toshikazu Sana , Akihito Sakai , Akiro Murai , Yasuhiro Takenaka , Kazuyoshi Kawano , Tomoya Takahashi
IPC分类号： B29D99/00
CPC分类号： B29C70/30 , B29C33/0011 , B29C43/3642 , B29C70/32 , B29C70/342 , B29C70/543 , B29C2043/3652 , B29C2043/3655 , B29D99/001 , B29K2995/0008 , B29L2031/3082 , B64F5/10 , Y02T50/43 , Y10T156/103
摘要： A mold (10) according to the present invention is a mold for molding a surface of a laminate (60) when curing the laminate (60) obtained by laminating prepreg (62). The mold (10) has a planar plate shape and elastically deforms from the planar plate shape into a shape corresponding to a shape of the laminate (60), thereby being capable of coming tight into contact with the laminate (60). Thereby, a mold which is easy to handle can be provided.
摘要翻译：根据本发明的模具（10）是用于在固化通过层压预浸料坯（62）获得的层压体（60）时模制层压体（60）的表面的模具。模具（10）具有平板形状，并且从平板形状弹性变形成与层压体（60）的形状相对应的形状，从而能够紧密地与层压体（60）接触。由此，可以提供容易处理的模具。

8. 发明授权

US07316916B2 DNA for encoding D-hydantoin hydrolases, DNA for encoding N-carbamyl-D-amino acid hydrolases, recombinant DNA containing the genes, cells transformed with the recombinant DNA, methods for producing proteins utilizing the transformed cells and methods for producing D-amino acids 失效
标题翻译：用于编码D-乙内酰脲水解酶的DNA，用于编码N-氨基甲酰-D-氨基酸水解酶的DNA，含有这些基因的重组DNA，用重组DNA转化的细胞，利用转化细胞产生蛋白质的方法和用于产生D-氨基酸的方法
公开(公告)号：US07316916B2
公开(公告)日：2008-01-08
申请号：US11433467
申请日：2006-05-15
申请人： Yasuhiro Takenaka , Ikuo Kira , Kenzo Yokozeki
发明人： Yasuhiro Takenaka , Ikuo Kira , Kenzo Yokozeki
IPC分类号： C12P13/04 , C12N9/10 , C12N1/21 , C12N15/63 , C07H21/04
CPC分类号： C12N9/86 , C12N9/80 , C12P13/04
摘要： DNA for encoding a protein having D-hydantoinase activity which has a base sequence represented by Sequence ID No. 1 in the Sequence Listing. DNA for encoding a protein having D-carbamylase activity which has a base sequence represented by Sequence ID No. 3 in the Sequence Listing.
摘要翻译：用于编码具有D-乙内酰脲酶活性的蛋白质的DNA，其具有由序列表中序列号1表示的碱基序列。用于编码具有D-氨基甲酰酶活性的蛋白质的DNA，其具有由序列表中的序列号3表示的碱基序列。

9. 发明申请

US20050112729A1 Recombinant DNA having hydantoinase gene and carbamylase gene and process for producing amino acid 审中-公开
标题翻译：具有乙内酰脲酶基因和氨基甲酰酶基因的重组DNA及其生产氨基酸的方法
公开(公告)号：US20050112729A1
公开(公告)日：2005-05-26
申请号：US10961116
申请日：2004-10-12
申请人： Ikuo Kira , Yasuhiro Takenaka , Hiroyuki Nozaki , Kunihiko Watanabe
发明人： Ikuo Kira , Yasuhiro Takenaka , Hiroyuki Nozaki , Kunihiko Watanabe
IPC分类号： C12N1/21 , C12N9/80 , C12N9/86 , C12N15/71 , C12P13/04
CPC分类号： C12N9/80 , C12N9/86 , C12N15/71 , C12P13/04
摘要： The object of the present invention is to prepare a recombinant DNA efficiently co-expressing genes of enzymes such as HHase, and to produce an amino acid efficiently from hydantoin by utilizing such a recombinant DNA. The present invention provides a recombinant DNA having base sequences encoding the following gene regions (I) and (II) incorporated therein in a predetermined direction and in a predetermined order to produce an amino acid efficiently: (I) a gene region containing a base sequence encoding a hydantoinase gene and a trp promoter sequence located upstream of the base sequence encoding the hydantoinase gene regulating expression of the hydantoinase gene, and (II) a gene region containing a base sequence encoding a carbamylase gene and a trp promoter sequence located upstream of the base sequence encoding the carbamylase gene regulating expression of the carbamylase gene.
摘要翻译：本发明的目的是制备有效共同表达诸如HHase的酶的基因的重组DNA，并通过利用这种重组DNA从乙内酰脲有效地产生氨基酸。本发明提供一种具有编码下述基因区（I）和（II）的碱基序列的重组DNA，其特征在于，在规定的方向和规定的顺序中并入其中，以有效地产生氨基酸：（I）含有碱基序列编码乙内酰脲酶基因和位于编码修饰乙内酰脲酶基因表达的乙内酰脲酶基因的碱基序列上游的trp启动子序列，以及（II）含有编码氨基甲酰化酶基因的碱基序列和位于上游的trp启动子序列的基因区域编码调节氨基甲酰酶基因表达的氨基甲酰酶基因的碱基序列。

10. 发明授权

US09409361B2 Mold, molding jig and molding method 有权
标题翻译：模具，成型夹具和成型方法
公开(公告)号：US09409361B2
公开(公告)日：2016-08-09
申请号：US13976732
申请日：2011-12-26
申请人： Toshikazu Sana , Akihito Sakai , Akira Murai , Yasuhiro Takenaka , Kazuyoshi Kawano , Tomoya Takahashi
发明人： Toshikazu Sana , Akihito Sakai , Akira Murai , Yasuhiro Takenaka , Kazuyoshi Kawano , Tomoya Takahashi
IPC分类号： B29C43/36 , B29C70/32 , B29D99/00 , B29C33/00 , B29C70/54 , B64F5/00
CPC分类号： B29C70/30 , B29C33/0011 , B29C43/3642 , B29C70/32 , B29C70/342 , B29C70/543 , B29C2043/3652 , B29C2043/3655 , B29D99/001 , B29K2995/0008 , B29L2031/3082 , B64F5/10 , Y02T50/43 , Y10T156/103
摘要： A mold (10) according to the present invention is a mold for molding a surface of a laminate (60) when curing the laminate (60) obtained by laminating prepreg (62). The mold (10) has a planar plate shape and elastically deforms from the planar plate shape into a shape corresponding to a shape of the laminate (60), thereby being capable of coming tight into contact with the laminate (60). Thereby, a mold which is easy to handle can be provided.
摘要翻译：根据本发明的模具（10）是用于在固化通过层压预浸料坯（62）获得的层压体（60）时模制层压体（60）的表面的模具。模具（10）具有平板形状，并且从平板形状弹性变形成与层压体（60）的形状相对应的形状，从而能够紧密地与层压体（60）接触。由此，能够提供容易处理的模具。

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式