专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

1. 发明授权

US06712612B1 Safe and stable retroviral helper cell line and related compositions and methods 有权
标题翻译：安全稳定的逆转录病毒辅助细胞系及相关组合物和方法
公开(公告)号：US06712612B1
公开(公告)日：2004-03-30
申请号：US10020669
申请日：2001-12-12
申请人： Frank Yao Tsung Tung
发明人： Frank Yao Tsung Tung
IPC分类号： C12P2106
CPC分类号： C12N15/86 , C07H21/04 , C12N2740/15052
摘要： A safe gene transfer system is provided based on simian immunodeficiency virus (SIV). A packaging construct is provided in which the SIV packaging sequence is removed, and, in one instance, the gag, pol and other accessory genes are expressed under the control of CMV promoter. SIV transfer constructs are provided in which cis-acting sequences required for viral replication (R, tRNA primer binding site, encapsidation site, RRE sequence and 3′ LTR), with or without a marker gene, are described. Stable packaging cell lines are provided that were prepared by transfecting 293(T) cells with an SIV packaging construct. High titer virus (>107 transducing units/ml) can be produced upon transient transfection with SIV transfer vectors. The safety of the described SIV gene transfer vectors was further tested in a primate model. Rhesus monkeys that received multiple inoculations of high titer (107) retroviral vectors did not develop any signs of side effects.
摘要翻译：基于猿猴免疫缺陷病毒（SIV）提供了一个安全的基因转移系统。提供其中除去SIV包装序列的包装结构，并且在一种情况下，gag，pol和其他辅助基因在CMV启动子的控制下表达。提供了SIV转移构建体，其中描述了具有或不具有标记基因的病毒复制所需的顺式作用序列（R，tRNA引物结合位点，壳化位点，RRE序列和3'LTR）。提供稳定的包装细胞系，其通过用SIV包装构建体转染293（T）细胞而制备。用SIV转移载体瞬时转染后可产生高效价病毒（> 10 7转导单位/ ml）。在灵长类动物模型中进一步测试所述SIV基因转移载体的安全性。接受高滴度（10 7）逆转录病毒载体的多次接种的恒河猴没有发生任何副作用的迹象。

IPRDB

热门服务

关于我们

友情链接

联系方式