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1. 发明申请

US20050142543A1 Method of designing addressable array for detection of nucleic acid sequence differences using ligase detection reaction 有权
标题翻译：使用连接酶检测反应设计用于检测核酸序列差异的可寻址阵列的方法
公开(公告)号：US20050142543A1
公开(公告)日：2005-06-30
申请号：US10257158
申请日：2001-04-04
申请人： Francis Barany , Monib Zirvi , Norman Gerry , Reyna Favis , Richard Kliman
发明人： Francis Barany , Monib Zirvi , Norman Gerry , Reyna Favis , Richard Kliman
IPC分类号： G01N33/53 , C12M1/00 , C12N15/09 , C12Q1/25 , G01N33/569 , G01N37/00 , C12Q1/68 , C12P19/34
CPC分类号： C12Q1/6837 , C12Q1/6827 , C12Q1/6874 , C12Q1/6886 , C12Q2527/107 , C12Q2521/501 , C12Q2521/319 , C12Q2565/501
摘要： The present invention is directed to a method of designing a plurality of capture oligonucleotide probes for use on a support to which complementary oligonucleotide probes will hybridize with little mismatch, where the plural capture oligonucleotide probes have melting temperatures within a narrow range. The first step of the method involves providing a first set of a plurality of tetramers of four nucleotides linked together, where (1) each tetramer within the set differs from all other tetramers in the set by at least two nucleotide bases, (2) no two tetramers within a set are complementary to one another, (3) no tetramers within a set are palindromic or dinucleotide repeats, and (4) no tetramer within a set has one or less or three or more G or C nucleotides. Groups of 2 to 4 of the tetramers from the first set are linked together to form a collection of multimer units. From the collection of multimer units, all multimer units formed from the same tetramer and all multimer units having a melting temperature in ° C. of less than 4 times the number of tetramers forming a multimer unit are removed to form a modified collection of multimer units. The modified collection of multimer units is arranged in a list in order of melting temperature. The order of the modified collection of multimer units is randomized in 2° C. increments of melting temperature.
摘要翻译：本发明涉及一种设计多个捕获寡核苷酸探针的方法，所述多个捕获寡核苷酸探针用于互补寡核苷酸探针与少量错配杂交的载体上，其中多个捕获寡核苷酸探针具有在窄范围内的熔融温度。该方法的第一步包括提供连接在一起的四个核苷酸的多个四聚体的第一组，其中（1）组中的每个四聚体与组中的所有其他四聚体不同，至少两个核苷酸碱基，（2）否一组内的两个四聚体彼此互补，（3）一组内的四聚体没有回归或二核苷酸重复，以及（4）组内的四聚体没有一个或多个或三个或更多个G或C核苷酸。来自第一组的4至4个四聚体的组连接在一起以形成多聚体单元的集合。从多聚体单元的收集中，除去由相同的四聚体形成的所有多聚体单元和具有小于形成多聚体单元的四聚体数目的4倍的熔点在℃℃的所有多聚体单元以形成多聚体单元的修饰的聚集体。修改的多聚体单元的集合按照熔融温度的顺序排列在列表中。修改的多聚体单元收集的顺序在2℃的熔融温度增量下随机化。

2. 发明授权

US08492085B2 Method of designing addressable array suitable for detection of nucleic acid sequence differences using ligase detection reaction 有权
标题翻译：使用连接酶检测反应设计适用于检测核酸序列差异的可寻址阵列的方法
公开(公告)号：US08492085B2
公开(公告)日：2013-07-23
申请号：US12252169
申请日：2008-10-15
申请人： Francis Barany , Monib Zirvi , Norman P. Gerry , Reyna Favis , Richard Kliman
发明人： Francis Barany , Monib Zirvi , Norman P. Gerry , Reyna Favis , Richard Kliman
IPC分类号： C12Q1/68 , C12M1/00 , C12M1/34 , C12M3/00
CPC分类号： C12Q1/6837 , C12Q1/6827 , C12Q1/6874 , C12Q1/6886 , C12Q2527/107 , C12Q2521/501 , C12Q2521/319 , C12Q2565/501
摘要： The present invention is directed to a method of designing a plurality of capture oligonucleotide probes for use on a support to which complementary oligonucleotide probes will hybridize with little mismatch, where the plural capture oligonucleotide probes have melting temperatures within a narrow range. The first step of the method involves providing a first set of a plurality of tetramers of four nucleotides linked together, where (1) each tetramer within the first set differs from all other tetramers in the first set by at least two nucleotide bases, (2) no two tetramers within the first set are complementary to one another, (3) no tetramers within the first set are palindromic or dinucleotide repeats, and (4) no tetramer within the first set has one or less or three or more G or C nucleotides. Groups of 2 to 4 of the tetramers from the first set are linked together to form a collection of multimer units. From the collection of multimer units, all multimer units formed from the same tetramer and all multimer units having a melting temperature in .degree. C. of less than 4 times the number of tetramers forming a multimer unit are removed to form a modified collection of multimer units. The modified collection of multimer units is arranged in a list in order of melting temperature. The order of the modified collection of multimer units is randomized in 2.degree. C. increments of melting temperature.
摘要翻译：本发明涉及一种设计多个捕获寡核苷酸探针的方法，所述多个捕获寡核苷酸探针用于互补寡核苷酸探针与少量错配杂交的载体上，其中多个捕获寡核苷酸探针具有在窄范围内的熔融温度。该方法的第一步涉及提供连接在一起的四个核苷酸的多个四聚体的第一组，其中（1）第一组中的每个四聚体与第一组中的所有其它四聚体相差至少两个核苷酸碱基（2 ）第一组中没有两个四聚体彼此互补，（3）第一组中没有四聚体是回文或二核苷酸重复，和（4）第一组中没有四聚体具有一个或更少或三个或更多个G或C 核苷酸。来自第一组的4至4个四聚体的组连接在一起以形成多聚体单元的集合。从多聚体单元的收集中，由相同四聚体形成的所有多聚体单元和具有等温熔融温度的所有多聚体单元。去除形成多聚体单元的四聚体数目的小于4倍的C.形成多聚体单元的修改的集合。修改的多聚体单元的集合按照熔融温度的顺序排列在列表中。修饰的多聚体单位收集的顺序是随机分为2度。 C.熔化温度升高。

3. 发明授权

US07455965B2 Method of designing addressable array for detection of nucleic acid sequence differences using ligase detection reaction 有权
标题翻译：使用连接酶检测反应设计用于检测核酸序列差异的可寻址阵列的方法
公开(公告)号：US07455965B2
公开(公告)日：2008-11-25
申请号：US10257158
申请日：2001-04-04
申请人： Francis Barany , Monib Zirvi , Norman P. Gerry , Reyna Favis , Richard Kliman
发明人： Francis Barany , Monib Zirvi , Norman P. Gerry , Reyna Favis , Richard Kliman
IPC分类号： C12Q1/68 , C12P19/34
CPC分类号： C12Q1/6837 , C12Q1/6827 , C12Q1/6874 , C12Q1/6886 , C12Q2527/107 , C12Q2521/501 , C12Q2521/319 , C12Q2565/501
摘要： The present invention is directed to a method of designing a plurality of capture oligonucleotide probes for use on a support to which complementary oligonucleotide probes will hybridize with little mismatch, where the plural capture oligonucleotide probes have melting temperatures within a narrow range. The first step of the method involves providing a first set of a plurality of tetramers of four nucleotides linked together, where (1) each tetramer within the first set differs from all other tetramers in the first set by at least two nucleotide bases, (2) no two tetramers within the first set are complementary to one another, (3) no tetramers within the first set are palindromic or dinucleotide repeats, and (4) no tetramer within the first set has one or less or three or more G or C nucleotides. Groups of 2 to 4 of the tetramers from the first set are linked together to form a collection of multimer units. From the collection of multimer units, all multimer units formed from the same tetramer and all multimer units having a melting temperature in ° C. of less than 4 times the number of tetramers forming a multimer unit are removed to form a modified collection of multimer units. The modified collection of multimer units is arranged in a list in order of melting temperature. The order of the modified collection of multimer units is randomized in 2° C. increments of melting temperature.
摘要翻译：本发明涉及一种设计多个捕获寡核苷酸探针的方法，所述多个捕获寡核苷酸探针用于互补寡核苷酸探针与少量错配杂交的载体上，其中多个捕获寡核苷酸探针具有在窄范围内的熔融温度。该方法的第一步涉及提供连接在一起的四个核苷酸的多个四聚体的第一组，其中（1）第一组中的每个四聚体与第一组中的所有其它四聚体相差至少两个核苷酸碱基（2 ）第一组中没有两个四聚体彼此互补，（3）第一组中没有四聚体是回文或二核苷酸重复，和（4）第一组中没有四聚体具有一个或更少或三个或更多个G或C 核苷酸。来自第一组的4至4个四聚体的组连接在一起以形成多聚体单元的集合。从多聚体单元的收集中，除去由相同的四聚体形成的所有多聚体单元和具有小于形成多聚体单元的四聚体数目的4倍的熔点在℃℃的所有多聚体单元以形成多聚体单元的修饰的聚集体。修改的多聚体单元的集合按照熔融温度的顺序排列在列表中。修改的多聚体单元收集的顺序在2℃的熔融温度增量下随机化。

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