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1. 发明授权

US07115403B1 Directed evolution of galactose oxidase enzymes 失效
标题翻译：半乳糖氧化酶的定向进化
公开(公告)号：US07115403B1
公开(公告)日：2006-10-03
申请号：US09571553
申请日：2000-05-16
申请人： Frances H. Arnold , Ioanna P. Petrounia , Lianhong Sun
发明人： Frances H. Arnold , Ioanna P. Petrounia , Lianhong Sun
IPC分类号： C12N9/02 , C12N1/20 , C12N15/00 , C12Q1/68 , C12Q1/00 , C07H21/04 , C12P21/04
CPC分类号： C12N9/0006 , C12N15/102
摘要： This invention relates to the expression of improved polynucleotide and polypeptide sequences encoding for eukaryotic enzymes, particularly oxidase enzymes. The enzymes are advantageously produced in conventional or facile expression systems. Various methods for directed evolution of polynucleotide sequences can be used to obtain the improved sequences. The improved characteristics of the polypeptides or proteins generated in this manner include improved expression, enhanced activity toward one or more substrates, and increased thermal stability. In a particular embodiment, the invention relates to improved expression of the galactose oxidase gene and galactose oxidase enzymes. GAO mutants that are highly active and/or thermostable are disclosed.
摘要翻译：本发明涉及编码真核生物酶，特别是氧化酶的改良的多核苷酸和多肽序列的表达。酶有利地在常规或简单的表达系统中产生。可以使用用于多核苷酸序列的定向进化的各种方法来获得改进的序列。以这种方式产生的多肽或蛋白质的改进特征包括改进的表达，增强对一种或多种底物的活性和增加的热稳定性。在一个具体实施方案中，本发明涉及半乳糖氧化酶基因和半乳糖氧化酶的改进表达。公开了高活性和/或热稳定的GAO突变体。

2. 发明授权

US07098010B1 Directed evolution of oxidase enzymes 失效
标题翻译：氧化酶的定向进化
公开(公告)号：US07098010B1
公开(公告)日：2006-08-29
申请号：US09722602
申请日：2000-11-27
申请人： Frances H. Arnold , Ioanna P. Petrounia , Lianhong Sun
发明人： Frances H. Arnold , Ioanna P. Petrounia , Lianhong Sun
IPC分类号： C12N9/02 , C12N1/20 , C12N15/00 , C12Q1/68 , C12Q1/00 , C12P21/04 , C07H21/04
CPC分类号： C12N9/0006 , C12N15/102
摘要： This invention relates to the expression of improved polynucleotide and polypeptide sequences encoding for eukaryotic enzymes, particularly oxidase enzymes. The enzymes are advantagoeusly produced in conventional or facile expression systems. Various methods for directed evolution of polynucleotide sequences can be used to obtain the improved sequences. The improved characteristics of the polypeptides or proteins generated in this manner include improved expression, enhanced activity toward one or more substrates, and increased thermal stability. In a particular embodiment, the invention relates to improved expression of the galactose oxidase gene and galactose oxidase enzymes. GAO mutants that are highly active and/or thermostable are disclosed.
摘要翻译：本发明涉及编码真核生物酶，特别是氧化酶的改良的多核苷酸和多肽序列的表达。这些酶在常规或简单的表达系统中有利地产生。可以使用用于多核苷酸序列的定向进化的各种方法来获得改进的序列。以这种方式产生的多肽或蛋白质的改进特征包括改进的表达，增强对一种或多种底物的活性和增加的热稳定性。在一个具体实施方案中，本发明涉及半乳糖氧化酶基因和半乳糖氧化酶的改进表达。公开了高活性和/或热稳定的GAO突变体。

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