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1. 发明授权

US5783453A Non-separation specific binding chemiluminescent assay 失效
标题翻译：非分离特异性结合化学发光测定
公开(公告)号：US5783453A
公开(公告)日：1998-07-21
申请号：US496549
申请日：1995-06-29
申请人： Eve H. Barlow , Eddie Carroll, III , Joseph E. Connolly , Michael J. Lee , Richard A. Martinelli , John T. Unger
发明人： Eve H. Barlow , Eddie Carroll, III , Joseph E. Connolly , Michael J. Lee , Richard A. Martinelli , John T. Unger
IPC分类号： G01N33/542 , G01N33/543 , G01N33/58 , C12G1/00 , G01N33/551 , G01N33/553
CPC分类号： G01N33/542 , G01N33/582
摘要： The assay described herein is predicated on an observation that when acridinium ester labeled tracers are bound to their corresponding binding conjugate immobilized on a metal oxide solid phase, the measurable chemiluminescent light emission of the labeled tracer bound to the solid phase is quenched as compared to the free fraction tracer that is unattached to the solid phase. According to the invention, a non-separation specific binding assay to detect or quantify the presence of an analyte is provided where the entire reaction mixture is flashed (including unreacted tracer) and modulated signal (because of the quench effect) is associated with a reference, thus determining the amount or presence of said analyte in said sample. Disadvantages inherent in heterogeneous assays employing multiple separations may be avoided using this non-separation method.
摘要翻译：本文所述的测定是基于以下观察结果：当吖啶酯标记的示踪剂与其固定在金属氧化物固相上的相应结合缀合物结合时，与固相结合的标记示踪剂的可测量化学发光光发射与与固相无关的游离级分示踪剂。根据本发明，提供了检测或定量分析物存在的非分离特异性结合测定法，其中整个反应混合物闪蒸（包括未反应的示踪剂）和调制信号（由于骤冷作用）与参考文献相关联，从而确定所述样品中所述分析物的量或存在。使用这种非分离方法可以避免使用多次分离的异质测定中固有的缺点。

2. 发明授权

US5705338A Reduction of background in noncompetitive binding assays 失效
标题翻译：减少非竞争性结合测定中的背景
公开(公告)号：US5705338A
公开(公告)日：1998-01-06
申请号：US434743
申请日：1995-05-04
申请人： Uri Piran , Laurie Ann Livshin , Richard A. Martinelli , William J. Riordan , John T. Unger
发明人： Uri Piran , Laurie Ann Livshin , Richard A. Martinelli , William J. Riordan , John T. Unger
IPC分类号： C12Q1/68 , G01N33/00 , G01N33/543 , G01N33/53 , G01N33/547
CPC分类号： C12Q1/6813 , C12Q1/6834 , G01N33/54306 , G01N2033/008
摘要： Novel non-competitive assay techniques have been developed which not only improve sensitivity, but also are convenient and less susceptible to interfering factors. They are compatible with existing instruments and can be run in one or more test tubes. The analyte is reacted with labeled specific binder, after which the mixture is reacted with (1) an insoluble material attached to an analyte derivative and (2) a solid phase carrying a binder. The solid phase is then separated, and the label attached to the solid phase is measured. Variations of the procedure include the use of a reversible bridge for attaching the insoluble material to the analyte mimic and the conduct of the assay in various porous media, such as paper, chromatographic and electrophoretic media, and dipsticks.
摘要翻译：已经开发了新的非竞争性测定技术，其不仅提高灵敏度，而且方便且不易受干扰因素的影响。它们与现有仪器兼容，可以在一个或多个试管中运行。分析物与标记的特异性粘合剂反应，之后混合物与（1）与分析物衍生物连接的不溶性物质和（2）携带粘合剂的固相反应。然后分离固相，测量附着于固相的标记。方法的变化包括使用可逆桥接器将不溶性物质连接到分析物模拟物上，以及在各种多孔介质如纸张，色谱和电泳介质以及浸渍条中进行测定。

3. 发明授权

US5959095A Amplification of midivariant DNA templates 失效
公开(公告)号：US5959095A
公开(公告)日：1999-09-28
申请号：US352670
申请日：1994-12-09
申请人： Richard A. Martinelli , Jeffrey J. Donahue , John T. Unger
发明人： Richard A. Martinelli , Jeffrey J. Donahue , John T. Unger
IPC分类号： C07H21/04 , C12N15/09 , C12Q1/68 , C12P19/34 , C12Q1/70
CPC分类号： C12Q1/682 , C12Q1/6867
摘要： New methods are provided for the amplification of a midivariant DNA containing an inserted target specific nucleic acid sequence(s) to enable detection of the presence of a target nucleic acid sequence in a test sample. One method employs midivariant DNA as a template for the synthesis of RNA catalyzed by QB replicase. Two midivariant DNA/probe conjugates including a nonreplicable portion of midivariant DNA and a target specific nucleic acid sequence (probe) are described. An amplification method including the steps of hybridization and ligation of the midivariant DNA/probe conjugates followed by replication of the DNA template has enabled detection of less than 200 template molecules. In a modified amplification method one of the midivariant DNA/probe conjugates further includes a RNA polymerase promoter sequence to enable transcription of the midivariant DNA template into RNA. The sequential ligation-transcription-replication method enables the detection of a single template molecule. The detectable molecule(s) are indicative of the presence of the target nucleic acid sequences in the test sample.

4. 发明授权

US5407798A Amplification of midivariant DNA templates 失效
标题翻译：多变量DNA模板的扩增
公开(公告)号：US5407798A
公开(公告)日：1995-04-18
申请号：US15249
申请日：1993-02-05
申请人： Richard A. Martinelli , Jeffrey J. Donahue , John T. Unger
发明人： Richard A. Martinelli , Jeffrey J. Donahue , John T. Unger
IPC分类号： C07H21/04 , C12N15/09 , C12Q1/68 , C12P19/34
CPC分类号： C12Q1/682 , C12Q1/6867
摘要： New methods are provided for the amplification of a midivariant DNA containing an inserted target specific nucleic acid sequence(s) to enable detection of the presence of a target nucleic acid sequence in a test sample. One method employs midivariant DNA as a template for the synthesis of RNA catalyzed by QB replicase. Two midivariant DNA/probe conjugates including a nonreplicable portion of midivariant DNA and a target specific nucleic acid sequence (probe) are described. An amplification method including the steps of hybridization and ligation of the midivariant DNA/probe conjugates followed by replication of the DNA template has enabled detection of less than 200 template molecules. In a modified amplification method one of the midivariant DNA/probe conjugates further includes a RNA polymerase promoter sequence to enable transcription of the midivariant DNA template into RNA. The sequential ligation-transcription-replication method enables the detection of a single template molecule. The detectable molecule(s) are indicative of the presence of the target nucleic acid sequences in the test sample.
摘要翻译：提供了用于扩增含有插入的靶特异性核酸序列的多变性DNA的新方法，以便能够检测测试样品中靶核酸序列的存在。一种方法采用半变体DNA作为模板，用于合成由QB复制酶催化的RNA。描述了包括不可复制部分的变形DNA和靶特异性核酸序列（探针）的两个变异型DNA /探针缀合物。包括以下步骤的扩增方法，其中杂交和连接半变异DNA /探针缀合物，随后DNA模板的复制使得能够检测到少于200个模板分子。在修饰的扩增方法中，其中一个变体DNA /探针结合物进一步包括RNA聚合酶启动子序列，以使得能够将该变体DNA模板转录成RNA。顺序连接 - 转录复制方法能够检测单个模板分子。可检测分子指示测试样品中靶核酸序列的存在。

5. 发明授权

US4434227A Immunoassay for class specific immunoglobulin antibodies 失效
标题翻译：针对类别特异性免疫球蛋白抗体的免疫测定
公开(公告)号：US4434227A
公开(公告)日：1984-02-28
申请号：US346662
申请日：1982-02-08
申请人： John T. Unger
发明人： John T. Unger
IPC分类号： G01N33/53 , G01N33/543 , G01N33/68 , G01N33/54
CPC分类号： G01N33/6854 , Y10S435/962 , Y10S436/825
摘要： A method for determining an immunoglobulin of the IgX class in a sample where X is either M, A, D or E. Anti-IgG is added to IgG to prevent binding of rheumatoid factor before the sample containing IgX is added to insolubilized IgG.
摘要翻译：用于确定X是M，A，D或E的样品中IgX类免疫球蛋白的方法。将抗IgG加入到IgG中，以防止在将含有IgX的样品加入到不溶性IgG中之前结合类风湿因子。

6. 发明授权

US5763186A Use of antisense oligomers in a process for controlling contamination in nucleic acid amplification reactions 失效
标题翻译：在控制核酸扩增反应污染的过程中使用反义寡聚体
公开(公告)号：US5763186A
公开(公告)日：1998-06-09
申请号：US778702
申请日：1997-01-03
申请人： Douglas N. Ludtke , John E. Monahan , John T. Unger
发明人： Douglas N. Ludtke , John E. Monahan , John T. Unger
IPC分类号： C12N15/00 , C12Q1/68 , C07H21/04 , C12N7/00 , C12P19/34
CPC分类号： C12Q1/6848 , C12Q1/682 , C12Q1/6867
摘要： A novel process for the use of antisense oligonucleotides and analogs thereof has been developed. Namely, this technique is useful for the elimination of contamination in the nucleic acid amplification area. Elimination of unwanted contamination has made gene probe analyses much more reproduceable.
摘要翻译：已经开发了一种使用反义寡核苷酸及其类似物的新方法。也就是说，该技术对于消除核酸扩增区域中的污染是有用的。消除不想要的污染使基因探针分析更加可重现。

7. 发明授权

US5702887A Long emission wavelength chemiluminescent compounds and their use in test assays 失效
标题翻译：长发射波长化学发光化合物及其在测试测定中的应用
公开(公告)号：US5702887A
公开(公告)日：1997-12-30
申请号：US340093
申请日：1994-11-14
申请人： Say-Jong Law , Qingping Jiang , Walter Fischer , John T. Unger , Elizabeth K. Krodel
发明人： Say-Jong Law , Qingping Jiang , Walter Fischer , John T. Unger , Elizabeth K. Krodel
IPC分类号： G01N33/50 , C07D219/04 , C07D221/18 , C12Q1/68 , G01N21/78 , G01N33/532 , G01N33/58 , C09K11/00 , G01N33/53
CPC分类号： C07D221/18 , C12Q1/6816 , G01N33/582 , Y10S435/968 , Y10S436/805
摘要： An assay method incorporating at least two different chemiluminescent compounds for detection and/or quantitation of at least two substances in a test sample is described. The synthesis of chemiluminescent reagents or conjugates for use in such methods as well as kits incorporating such reagents are also disclosed. The assays have particular application in the field of clinical diagnostics.
摘要翻译：描述了包含至少两种不同的化学发光化合物用于检测和/或定量测试样品中至少两种物质的测定方法。还公开了用于这些方法的化学发光试剂或缀合物的合成以及掺入这些试剂的试剂盒。该测定法在临床诊断领域具有特殊应用。

8. 发明申请

US20120222668A1 Collapsible Fire Pit and Propane Tank Holder 审中-公开
标题翻译：可折叠火坑和丙烷罐架
公开(公告)号：US20120222668A1
公开(公告)日：2012-09-06
申请号：US13039804
申请日：2011-03-03
申请人： John T. Unger
发明人： John T. Unger
IPC分类号： F24B1/181 , F24B1/192
CPC分类号： F24C3/14 , F24B1/181
摘要： A fire pit designed to be stored and shipped in a flat configuration, and assembled without tools in a matter of minutes into a freestanding, durable structure capable of functioning as well or better than a non-collapsible fire pit. The fire pit comprises an essentially flat base with a unique slot and flange structure adapted to receive and support side panels. The side panels include female sides with vertical corner members on their side edges that allow flat male sides to mate securely with the female sides, and with the intersecting edges of the sides both covered and reinforced for a neat appearance and robust structure. A top includes a slot and flange structure that fits over and unifies and hides the upper edges of the sides, completing the assembly.
摘要翻译：一个防火坑被设计成以平坦的形式储存和运输，并且在几分钟之内就没有工具组装成独立的，耐用的结构，能够比不可折叠的火坑发挥好的作用。火坑包括基本上平坦的底座，其具有适于容纳和支撑侧板的独特的槽和凸缘结构。侧板包括在其侧边缘上具有垂直拐角构件的阴侧面，其允许平坦的阳侧与母体侧面牢固地配合，并且侧面的相交边缘被覆盖和加强，以便整洁的外观和坚固的结构。顶部包括狭槽和凸缘结构，其适合并且组合和隐藏侧边的上边缘，从而完成组装。

9. 发明申请

US20120040337A1 Conformational Epitope Initiated Signal Amplification 审中-公开
标题翻译：构象表位启动信号放大
公开(公告)号：US20120040337A1
公开(公告)日：2012-02-16
申请号：US13263367
申请日：2010-04-09
申请人： John T. Unger , Rolf Hilfiker
发明人： John T. Unger , Rolf Hilfiker
IPC分类号： G01N21/64 , C12Q1/70 , G01N21/75
CPC分类号： G01N33/557 , C12Q1/6804 , C12Q2565/101 , C12Q2525/205
摘要： This invention relates to a method to generate a signal used to detect the presence or quantity of a biomarker in a sample. The signal generating reaction is initiated when the biomarker under assay interacts with a specific binding partner. The interaction produces a structural change in the binding partner that is recognized by additional binding partners capable of generating a signal. The reaction produces a localized cluster of signaling molecules that can be detected above background. The signaling cluster is detectable within minutes when interrogated in a chamber of specific dimensions. The presence of the signaling clusters is a qualitative indication of the presence of the analyte, while the number of signaling clusters detected is a direct quantification of the number of biomarker molecules in the sample. The reaction can be formatted to detect proteins, nucleic acids, cells or other informative biomarkers.
摘要翻译：本发明涉及生成用于检测样品中生物标志物的存在或数量的信号的方法。当测定中的生物标志物与特异性结合配偶体相互作用时，起始信号产生反应。相互作用产生由能够产生信号的另外的结合配偶体识别的结合配偶体的结构变化。该反应产生可以在背景以上检测到的信号分子的局部簇。当在特定尺寸的室中询问时，信号簇在几分钟内可被检测。信号簇的存在是分析物存在的定性指示，而检测到的信号簇的数量是对样品中生物标记分子数量的直接定量。该反应可被格式化以检测蛋白质，核酸，细胞或其他信息生物标志物。

10. 发明授权

US5879894A Long emission wavelength chemiluminescent compounds and their use in test assays 失效
公开(公告)号：US5879894A
公开(公告)日：1999-03-09
申请号：US308772
申请日：1994-09-19
申请人： Say-Jong Law , Qingping Jiang , Walter Fischer , John T. Unger , Elizabeth K. Krodel , Jun Xi
发明人： Say-Jong Law , Qingping Jiang , Walter Fischer , John T. Unger , Elizabeth K. Krodel , Jun Xi
IPC分类号： G01N33/50 , C07D219/04 , C07D221/18 , C12Q1/68 , G01N21/78 , G01N33/532 , G01N33/58 , G01N33/53 , G01N21/76 , G01N33/536 , G01N33/566
CPC分类号： C07D221/18 , C12Q1/6816 , G01N33/582 , Y10S435/968 , Y10S436/805
摘要： An assay method incorporating at least two different chemiluminescent compounds for detection and/or quantitation of at least two substances in a test sample is described. The synthesis of chemiluminescent reagents or conjugates for use in such methods as well as kits incorporating such reagents are also disclosed. The assays have particular application in the field of clinical diagnostics.

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