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    • 4. 发明申请
    • METHODS FOR MULTIPART, MODULAR AND SCARLESS ASSEMBLY OF DNA MOLECULES
    • DNA分子多模,无模组装的方法
    • US20140038240A1
    • 2014-02-06
    • US13939110
    • 2013-07-10
    • Karsten TemmeAlvin Tamsir
    • Karsten TemmeAlvin Tamsir
    • C12P19/34
    • C12P19/34C12N15/10C12N15/66
    • The present invention consists of methods for joining DNA molecules (parts) together to form larger DNA molecules (assemblies) of specified sequence and organization. The invention exhibits three necessary characteristics. Firstly, the invention enables 2 or more parts to be joined in a single reaction. Secondly, the seam between joined parts is scarless, producing no residual sequence dependencies like restriction enzyme recognition sites. Thirdly, parts are modular and can easily be reused in novel assemblies without modification. Prior technologies have exhibited no more than two of the three necessary characteristics, limiting their utility in synthesizing and editing DNA molecules of arbitrary sequence.
    • 本发明包括将DNA分子(部分)连接在一起以形成具有特定序列和组织的较大DNA分子(组件)的方法。 本发明具有三个必要的特征。 首先,本发明能使2个以上的部件在一个反应​​中连接。 其次,接合部分之间的接缝是无缝的,不产生残留序列依赖性,如限制酶识别位点。 第三,部件是模块化的,并且可以容易地在新的组件中重新使用而不进行修改。 现有技术已经表现出三个必要特征中不超过两个,限制了它们在任意序列的DNA分子的合成和编辑中的应用。