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1. 发明授权

US5427941A Actinomadura brunnea var. antibiotica strains 失效
标题翻译：放线菌抗生素菌株
公开(公告)号：US5427941A
公开(公告)日：1995-06-27
申请号：US24896
申请日：1993-03-01
申请人： Elizabeth B. Smith , Hanan K. Munayyer , Michael J. Ryan , George H. Miller
发明人： Elizabeth B. Smith , Hanan K. Munayyer , Michael J. Ryan , George H. Miller
IPC分类号： C12P29/00 , C12N1/20
CPC分类号： C12R1/03 , C12P29/00 , C12R1/01 , Y10S435/822
摘要： 7-Chloro-8-methoxytetracycline isolated from antibiotic ES-119 and antibiotic Tet. 7 which are produced by fermentation of mutants of Actinomadura brunnea, namely Actinomadura brunnea var. antibiotica, ATCC 53108 and Actinomadura brunnea var antibiotica ATCC 53180 and its use as an antibiotic against gram-positive and gram-negative organisms are disclosed.
摘要翻译：从抗生素ES-119和抗生素Tet分离的7-氯-8-甲氧基四环素。 7，其是由放线杆菌突变体的发酵产生的，即放线杆菌（Actinomadura brunnea var。公开了抗生素，ATCC 53108和放线菌甘蓝抗菌素ATCC 53180及其作为抗革兰氏阳性和革兰氏阴性生物的抗生素的用途。

2. 发明授权

US5168040A Colorimetric assay for detecting fungal cell envelope perturbations 失效
标题翻译：用于检测真菌细胞包膜扰动的比色测定法
公开(公告)号：US5168040A
公开(公告)日：1992-12-01
申请号：US168831
申请日：1988-03-16
申请人： Michael J. Ryan , Jason A. Lotvin , Elizabeth B. Smith , Karen J. Shaw
发明人： Michael J. Ryan , Jason A. Lotvin , Elizabeth B. Smith , Karen J. Shaw
IPC分类号： C12Q1/02 , C12Q1/04
CPC分类号： C12Q1/02 , C12Q1/04 , C12Q2334/52 , G01N2333/395
摘要： Methods of constructing and using gene expression systems to indicate the pertubation of fungal cell envelopes are provided. The recombinant genes produce indicator cleavage enzymes such as .beta.-galactosidase when galactose is able to enter the cell, despite the presence of glucose, through discontinuous cell envelopes. The indicator cleavage enzymes such as .beta.-galactosidase cleaves a colorimetric indicator placed in the media to produce an easily detected measurement of altered cell envelope permeability.
摘要翻译：提供构建和使用基因表达系统来指示真菌细胞包膜的渗透的方法。当半乳糖通过不连续的细胞包膜能够进入细胞时，重组基因产生指示剂切割酶如β-半乳糖苷酶，尽管存在葡萄糖。指示剂裂解酶如β-半乳糖苷酶切割放置在培养基中的比色指示剂，以产生容易检测的改变的细胞包膜通透性的测量值。

3. 发明授权

US5866410A Cloning of the biosynthetic pathway for chlortetracycline and tetracycline formation and cosmids useful therein 失效
标题翻译：克隆金霉素和四环素形成的生物合成途径和其中有用的粘粒
公开(公告)号：US5866410A
公开(公告)日：1999-02-02
申请号：US474933
申请日：1995-06-07
申请人： Michael J. Ryan , Jason A. Lotvin , Nancy Strathy , Susan E. Fantini
发明人： Michael J. Ryan , Jason A. Lotvin , Nancy Strathy , Susan E. Fantini
IPC分类号： C12N15/52 , C12N15/76 , C12P29/00 , C12Q1/18 , C12N15/00
CPC分类号： C12N15/76 , C12N15/52 , C12P29/00 , C12Q1/18
摘要： The present invention describes a purified and isolated nucleic acid molecule which encodes for the biosynthetic pathway of tetracycline, chlortetracycline or an analogue thereof. The invention relates to the isolation and cloning of the nucleic acid molecule in an isolated fragment from Streptomyces aureofaciens and the expression of the biosynthetic gene in a heterologous host such as Streptomyces lividans.
摘要翻译：本发明描述了一种纯化和分离的核酸分子，其编码四环素，金霉素或其类似物的生物合成途径。本发明涉及从链霉菌（Streptomyces aureafaciens）分离的片段中分离和克隆核酸分子，以及在异源宿主如赤藓链霉菌中表达生物合成基因。

4. 发明授权

US5989903A Strain for the production of 6-dimethyltetracycline, method for producing the strain and vector for use in the method 失效
标题翻译：用于生产6-脱甲基四环素的菌株，生产菌株的方法和用于该方法的载体
公开(公告)号：US5989903A
公开(公告)日：1999-11-23
申请号：US487727
申请日：1995-06-07
申请人： Michael J. Ryan
发明人： Michael J. Ryan
IPC分类号： C12N15/74 , C12N15/68 , C12N15/52 , C12N15/76 , C12P29/00
CPC分类号： C12N15/74
摘要： Recombinant S. aureofaciens cells are provided. These cells comprise:(a) at least one CTC 11 gene; and (b) optionally(i) a CTC 09 gene;(ii) a CTC 03 gene; or(iii)a combination thereof;wherein:the CTC 11 gene is chromosomal, extra-chromosomal, or chromosomal and extra-chromosomal;the CTC 09 gene, CTC 03 gene, or a combination thereof is chromosomal, extra-chromosomal, or a combination thereof;expression of the CTC 11 gene is enhanced over that of a wild-type S. aureofaciens cell; andoptionally, the CTC 09 gene, the CTC 03 gene, or both of the CTC 09 gene and the CTC 03 gene are inactivated.The present invention also contemplates vector pLP21329 and vectors for allelic replacement in a S. aureofaciens host cell. The vectors comprise:(a) a functional E. coli origin of replication;(b) a functional Streptomyces origin of replication;(c) a functional gene that imparts a positively selectable phenotype on the host cell; and((d) a ribosomal S12 gene which is expressed in Streptomyces such that it imparts sensitivity to streptomycin to the host cell.In another embodiment, a method of mutating a target gene of a biosynthetic pathway of Streptomyces is disclosed. The method comprises(a) replacing the genomic copy of the target gene with a selectable marker gene through homologous recombination to form a first recombinant strain; and(b) replacing the selectable marker gene in the first recombinant strain with an altered copy of the target gene through homologous recombination to form a second recombinant strain.
摘要翻译：提供重组金黄色葡萄球菌细胞。这些细胞包含：（a）至少一种CTC 11基因; 和（b）任选地（i）CTC09基因; （ii）CTC03基因; 或（iii）其组合; 其中：CTC 11基因是染色体，染色体外或染色体和染色体外的; CTC09基因，CTC03基因或其组合是染色体，染色体外或其组合; CTC 11基因的表达比野生型金黄色葡萄球菌细胞的表达增强; 并且任选地，CTC09基因，CTC03基因或CTC09基因和CTC03基因两者都被灭活。本发明还考虑载体pLP21329和用于在金黄色葡萄球菌属宿主细胞中等位基因置换的载体。载体包括：（a）功能性大肠杆菌复制起点; （b）功能性链霉菌复制起点; （c）在宿主细胞上赋予正选择的表型的功能性基因; 公开了（d）在链霉菌中表达的核糖体S12基因，使得其对宿主细胞具有对链霉素的敏感性，在另一个实施方案中，公开了链霉菌生物合成途径的靶基因突变的方法，该方法包括（ a）通过同源重组用选择性标记基因替换目标基因的基因组拷贝以形成第一重组菌株;和（b）通过同源重组用靶基因的改变拷贝替换第一重组菌株中的选择性标记基因以形成第二重组菌株。

5. 发明授权

US4892743A Novel hybrid interferon species 失效
标题翻译：新型混合干扰素物种
公开(公告)号：US4892743A
公开(公告)日：1990-01-09
申请号：US564066
申请日：1983-12-21
申请人： Paul J. Leibowitz , Michael J. Ryan
发明人： Paul J. Leibowitz , Michael J. Ryan
IPC分类号： A61K38/00 , C07K14/56 , C12N15/72
CPC分类号： C12N15/72 , C07K14/56 , A61K38/00
摘要： The invention features a novel hybrid interferon species that comprises a chain of 161 and/or 162 amino acids. The hybrid is novel not only for its new structure, but also because the hybrid comprises a shortened or truncated segment of alpha interferon, and hence, an entirely new interferon species which is not occurring in nature.
摘要翻译：本发明的特征在于包含161个氨基酸和/或162个氨基酸的链的新型混合干扰素物质。该杂种不仅是新颖的结构，而且也是因为杂种包括缩短或截短的α干扰素片段，因此是一种全新的干扰素物质，它们不是在自然界中发生的。

6. 发明授权

US4389063A Reusable tamper-indicating security seal 失效
标题翻译：可重复使用的篡改表示安全密封
公开(公告)号：US4389063A
公开(公告)日：1983-06-21
申请号：US276611
申请日：1981-06-23
申请人： Michael J. Ryan
发明人： Michael J. Ryan
IPC分类号： G09F3/03 , B65D33/34
CPC分类号： G09F3/0394 , Y10S40/914 , Y10T292/48
摘要： The invention teaches means for detecting unauthorized tampering or substitutions of a device, and has particular utility when applied on a "seal" device used to secure a location or thing. The seal has a transparent body wall, and a first indicia, viz., a label identification is formed on the inside surface of this wall. Second and third indicia are formed on the outside surface of the transparent wall, and each of these indicia is transparent to allow the parallax angled viewing of the first indicia through these indicia. The second indicia is in the form of a broadly uniform pattern, viz, many small spaced dots; while the third indicia is in the form of easily memorized objects, such as human faces, made on a substrate by means of halftone printing. The substrate is lapped over the outside surface of the transparent wall. A thin cocoon of a transparent material, generally of the same material as the substrate such as plastic, is formed over the seal body and specifically over the transparent wall and the second and third indicia formed thereon. This cocoon is seamless and has walls of nonuniform thickness. Both the genuineness of the seal and whether anyone has attempted to compromise the seal can thus be visually determined upon inspection.
摘要翻译：本发明教导了用于检测未授权篡改或替换设备的装置，并且当应用于用于固定位置或物体的“密封”装置时具有特别的用途。密封件具有透明体壁，并且在该壁的内表面上形成第一标记，即标签识别。第二和第三标记形成在透明壁的外表面上，并且这些标记中的每一个都是透明的，以允许通过这些标记对第一标记进行视差成角度的观察。第二个标记是宽广均匀图案的形式，即许多小间隔的点; 而第三标记是通过半色调打印在基底上制成的容易记忆的物体（例如人脸）的形式。衬底被覆盖在透明壁的外表面上。透明材料的薄茧通常与诸如塑料的基底材料相同，形成在密封体上方，特别是形成在透明壁上，其上形成有第二和第三标记。这种茧是无缝的，具有不均匀厚度的墙壁。因此，密封件的真实性以及是否有人尝试妥协密封件，因此可以在检查后视觉上确定。

7. 发明授权

US4764024A Steam trap monitor 失效
标题翻译：蒸汽疏水阀监视器
公开(公告)号：US4764024A
公开(公告)日：1988-08-16
申请号：US45960
申请日：1987-05-04
申请人： Michael J. Ryan
发明人： Michael J. Ryan
IPC分类号： F16T1/48 , G01K17/06 , G01N25/60 , G01K11/00 , G01N25/20
CPC分类号： G01K17/06 , F16T1/48 , G01N25/60
摘要： A steam trap monitor positioned downstream of a steam trap in a closed steam system includes a first sensor (the combination of a hot finger and thermocouple well) for measuring the energy of condensate and a second sensor (a cold finger) for measuring the total energy of condensate and steam in the line. The hot finger includes one or more thermocouples for detecting condensate level and energy, while the cold finger contains a liquid with a lower boiling temperature than that of water. Vapor pressure from the liquid is used to do work such as displacing a piston or bellows in providing an indication of total energy (steam+condensate) of the system. Processing means coupled to and responsive to outputs from the thermocouple well hot and cold fingers subtracts the condensate energy as measured by the hot finger and thermocouple well from the total energy as measured by the cold finger to provide an indication of the presence of steam downstream from the trap indicating that the steam trap is malfunctioning.
摘要翻译：位于闭合蒸汽系统中的蒸汽疏水阀下游的蒸汽疏水阀监测器包括用于测量冷凝物能量的第一传感器（热指和热电偶井的组合）和用于测量总能量的第二传感器（冷指）的冷凝水和蒸汽在线。热指包括一个或多个用于检测冷凝物水平和能量的热电偶，而冷指包含沸点低于水的液体。使用来自液体的蒸汽压力来进行工作，例如移动活塞或波纹管，以提供系统的总能量（蒸汽+冷凝物）的指示。耦合到并响应于热电偶井和冷手指的输出的处理装置减去由热指和热电偶测得的冷凝能量与由冷指测量的总能量的距离，以提供下游的蒸汽的存在的指示疏水阀表明蒸汽疏水阀发生故障。

8. 发明授权

US4730385A Coax connector installation tool 失效
标题翻译：同轴连接器安装工具
公开(公告)号：US4730385A
公开(公告)日：1988-03-15
申请号：US028839
申请日：1987-03-23
申请人： Michael J. Ryan , David M. Minor
发明人： Michael J. Ryan , David M. Minor
IPC分类号： B25B13/48 , B25B21/00 , H01R9/05 , H01R43/00 , B23P19/04
CPC分类号： B25B21/002 , B25B13/48 , B25B21/007 , H01R43/00 , H01R9/0521 , Y10T29/49174 , Y10T29/53222
摘要： The invention is a tool to aid in the attaching of coaxial electrical cable ends to a half of a coaxial cable connector assembly. In detail, the tool comprises a wrench to prevent rotation between the connector halves when the connector halves are joined. A shaft is included having a threaded end for coupling to the recess of the other connector half. Finally, a drive preferably in the form of a portable electrical drill, is used to rotate the drive. Thus, with the joined connector halves installed in the wrench, so that rotation therebetween is prevented, the shaft can be threaded into the other connector half and thereafter coupled to the drill. The connector half to which the coaxial cable is to be joined is aligned with the prepared cable end and fed into the threaded recess as the wrench and joined connector halves are rotated.
摘要翻译：本发明是有助于将同轴电缆端部附接到同轴电缆连接器组件的一半的工具。具体地，该工具包括扳手以防止连接器半部连接时连接器半部之间的旋转。包括轴，其具有用于联接到另一个连接器半部的凹部的螺纹端。最后，使用优选以便携式电钻的形式的驱动器来旋转驱动器。因此，通过将连接的连接器半部安装在扳手中，从而防止它们之间的旋转，轴可以被拧入另一个连接器半部，并且此后连接到钻头。要将同轴电缆连接到的连接器一半与制备好的电缆端对齐，并作为扳手进给螺纹凹槽，并连接连接器半部。

9. 发明申请

US20110017739A1 Method and Container Lid to Manipulate A Container Opening Liner 失效
标题翻译：方法和容器盖以操纵容器开口衬里
公开(公告)号：US20110017739A1
公开(公告)日：2011-01-27
申请号：US12510058
申请日：2009-07-27
申请人： Andrew T. Tilton , Lanny D. Baker , Carl Feducia , Michael J. Ryan , James A. Whisenhunt
发明人： Andrew T. Tilton , Lanny D. Baker , Carl Feducia , Michael J. Ryan , James A. Whisenhunt
IPC分类号： B65D51/20
CPC分类号： B65D53/04 , B65D41/045
摘要： A container includes a body forming an interior and having a sidewall with a distal edge portion forming an opening to the interior. A liner is disposed over the opening and includes an inner portion and an outermost radial portion disposed radially outwardly of the distal edge portion. A lid including an interior surface and at least one tab projecting inward from the interior surface is disposed over the liner and engages the body. The liner is sized to be releasably rotatably secured within the lid while the lid is initially attached to the body so that the at least one tab remains generally rotationally fixed relative to the outermost radial portion while being assembled to the body. The at least one tab is arrange to press the outermost radial portion while the lid is being removed from the body so that the outermost radial portion maintains a bent orientation.
摘要翻译：容器包括形成内部并具有侧壁的主体，其具有形成到内部的开口的远侧边缘部分。衬套设置在开口上方，并且包括设置在远侧边缘部分的径向外侧的内部部分和最外侧径向部分。包括内表面和从内表面向内突出的至少一个突舌的盖设置在衬套上并与身体接合。衬套的尺寸被设计成可释放地可旋转地固定在盖内，同时盖最初附接到主体上，使得至少一个凸片在组装到主体的同时相对于最外侧的径向部分保持大致旋转地固定。至少一个突片布置成在盖子从主体移除的同时按压最外侧的径向部分，使得最外侧的径向部分保持弯曲取向。

10. 发明授权

US07092262B2 System and method for pre-charging the DC bus of a utility connected power converter 失效
标题翻译：对连接电力变压器的直流母线进行预充电的系统和方法
公开(公告)号：US07092262B2
公开(公告)日：2006-08-15
申请号：US10813550
申请日：2004-03-31
申请人： Michael J. Ryan , Mark G. Gilbreth , Kenneth W. Keller , Simon R. Wall
发明人： Michael J. Ryan , Mark G. Gilbreth , Kenneth W. Keller , Simon R. Wall
IPC分类号： H02H7/00 , G05F1/70
CPC分类号： H02M7/062
摘要： A system and method for pre-charging the DC bus of a utility connected power converter. In one embodiment, a full-wave rectifier circuit is coupled to the utility grid through an isolation transformer and circuit protector. The rectifier circuit output is connected to the DC bus of the power converter to provide a pre-charging current to the DC bus capacitors. The output from the rectifier circuit also supplies power to the control circuit power supply for the converter. When the control circuit closes the contactor of the converter system and activates the converter, the voltage across the DC bus capacitors is increased above the level of the rectified power grid voltage provided at the output of the pre-charge circuit. This reverse-biases the rectifier diodes, placing the pre-charge circuit into an inactive state. The impedance of the isolation transformer limits the in-rush current into the DC bus capacitors during the pre-charge.
摘要翻译：一种用于对连接电力转换器的直流母线进行预充电的系统和方法。在一个实施例中，全波整流电路通过隔离变压器和电路保护器耦合到公用电网。整流电路输出端连接到电源转换器的直流母线，为直流母线电容提供预充电电流。整流电路的输出也为转换器的控制电路电源供电。当控制电路关闭转换器系统的接触器并启动转换器时，直流总线电容器两端的电压增加到提供在预充电电路输出端的整流电网电压的电平。这反向偏置整流二极管，将预充电电路置于无效状态。隔离变压器的阻抗在预充电期间将冲击电流限制在直流总线电容器中。

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