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1. 发明申请

US20120184015A1 CYTOCHROME P450 OXYGENASES 有权
标题翻译： CYTOCHROME P450氧化剂
公开(公告)号：US20120184015A1
公开(公告)日：2012-07-19
申请号：US13323398
申请日：2011-12-12
申请人： Edgardo T. Farinas , Frances H. Arnold , Ulrich Schwaneberg , Anton Glieder
发明人： Edgardo T. Farinas , Frances H. Arnold , Ulrich Schwaneberg , Anton Glieder
IPC分类号： C12N9/02
CPC分类号： C12N9/0071 , C12P7/02 , C12P7/04 , C12P7/16 , C12P17/02 , Y02E50/10 , Y02P20/52
摘要： Nucleic acids encoding cytochrome P450 variants are provided. The cytochrome P450 variants of have a higher alkane-oxidation capability, alkene-oxidation capability, and/or a higher organic-solvent resistance than the corresponding wild-type or parent cytochrome P450 enzyme. A preferred wild-type cytochrome P450 is cytochrome P450 BM-3. Preferred cytochrome P450 variants include those having an improved capability to hydroxylate alkanes and epoxidate alkenes comprising less than 8 carbons, and have amino acid substitutions corresponding to V78A, H236Q, and E252G of cytochrome P450 BM-3. Preferred cytochrome P450 variants also include those having an improved hydroxylation activity in solutions comprising co-solvents such as DMSO and THF, and have amino acid substitutions corresponding to T235A, R471A, E494K, and S1024E of cytochrome P450 BM-3.
摘要翻译：提供了编码细胞色素P450变体的核酸。细胞色素P450变体具有比相应的野生型或亲本细胞色素P450酶更高的烷烃氧化能力，烯烃氧化能力和/或较高的有机溶剂耐受性。优选的野生型细胞色素P450是细胞色素P450 BM-3。优选的细胞色素P450变体包括具有改善含有少于8个碳原子的烷烃和环氧化烯烃羟基化能力以及对应于细胞色素P450 BM-3的V78A，H236Q和E252G的氨基酸取代的那些变体。优选的细胞色素P450变体还包括在包含共溶剂如DMSO和THF的溶液中具有改善的羟基化活性的那些，并且具有对应于细胞色素P450 BM-3的T235A，R471A，E494K和S1024E的氨基酸取代。

2. 发明授权

US07691616B2 Cytochrome P450 oxygenases 有权
标题翻译：细胞色素P450加氧酶
公开(公告)号：US07691616B2
公开(公告)日：2010-04-06
申请号：US11800970
申请日：2007-05-07
申请人： Edgardo T. Farinas , Frances H. Arnold , Ulrich Schwaneberg , Anton Glieder
发明人： Edgardo T. Farinas , Frances H. Arnold , Ulrich Schwaneberg , Anton Glieder
IPC分类号： C12N9/02 , C12N1/20 , C12N15/00 , C12Q1/26 , C12Q1/00 , C12P21/04 , C07H21/04 , C12Q1/68
CPC分类号： C12N9/0071 , C12P7/02 , C12P7/04 , C12P7/16 , C12P17/02 , Y02E50/10 , Y02P20/52
摘要： Nucleic acids encoding cytochrome P450 variants are provided. The cytochrome P450 variants of have a higher alkane-oxidation capability, alkene-oxidation capability, and/or a higher organic-solvent resistance than the corresponding wild-type or parent cytochrome P450 enzyme. A preferred wild-type cytochrome P450 is cytochrome P450 BM-3. Preferred cytochrome P450 variants include those having an improved capability to hydroxylate alkanes and epoxidate alkenes comprising less than 8 carbons, and have amino acid substitutions corresponding to V78A, H236Q, and E252G of cytochrome P450 BM-3. Preferred cytochrome P450 variants also include those having an improved hydroxylation activity in solutions comprising co-solvents such as DMSO and THF, and have amino acid substitutions corresponding to T235A, R471A, E494K, and S1024E of cytochrome P450 BM-3.
摘要翻译：提供了编码细胞色素P450变体的核酸。细胞色素P450变体具有比相应的野生型或亲本细胞色素P450酶更高的烷烃氧化能力，烯烃氧化能力和/或较高的有机溶剂耐受性。优选的野生型细胞色素P450是细胞色素P450 BM-3。优选的细胞色素P450变体包括具有改善含有少于8个碳原子的烷烃和环氧化烯烃羟基化能力以及对应于细胞色素P450 BM-3的V78A，H236Q和E252G的氨基酸取代的那些变体。优选的细胞色素P450变体还包括在包含共溶剂如DMSO和THF的溶液中具有改善的羟基化活性的那些，并且具有对应于细胞色素P450 BM-3的T235A，R471A，E494K和S1024E的氨基酸取代。

3. 发明授权

US07226768B2 Cytochrome P450 oxygenases 有权
标题翻译：细胞色素P450加氧酶
公开(公告)号：US07226768B2
公开(公告)日：2007-06-05
申请号：US10201213
申请日：2002-07-22
申请人： Edgardo T. Farinas , Frances H. Arnold , Ulrich Schwaneberg , Anton Glieder
发明人： Edgardo T. Farinas , Frances H. Arnold , Ulrich Schwaneberg , Anton Glieder
IPC分类号： C12N9/02 , C12N1/20 , C12N15/00 , C12P21/04 , C12Q1/34 , C12Q1/26 , C07H21/04 , C12Q1/00 , C12Q1/69
CPC分类号： C12N9/0071 , C12P7/02 , C12P7/04 , C12P7/16 , C12P17/02 , Y02E50/10 , Y02P20/52
摘要： Nucleic acids encoding cytochrome P450 variants are provided. The cytochrome P450 variants of have a higher alkane-oxidation capability, alkene-oxidation capability, and/or a higher organic-solvent resistance than the corresponding wild-type or parent cytochrome P450 enzyme. A preferred wild-type cytochrome P450 is cytochrome P450 BM-3. Preferred cytochrome P450 variants include those having an improved capability to hydroxylate alkanes and epoxidate alkenes comprising less than 8 carbons, and have amino acid substitutions corresponding to V78A, H236Q, and E252G of cytochrome P450 BM-3. Preferred cytochrome P450 variants also include those having an improved hydroxylation activity in solutions comprising co-solvents such as DMSO and THF, and have amino acid substitutions corresponding to T235A, R471A, E494K, and S1024E of cytochrome P450 BM-3.
摘要翻译：提供了编码细胞色素P450变体的核酸。细胞色素P450变体具有比相应的野生型或亲本细胞色素P450酶更高的烷烃氧化能力，烯烃氧化能力和/或较高的有机溶剂耐受性。优选的野生型细胞色素P450是细胞色素P450 BM-3。优选的细胞色素P450变体包括具有改善含有少于8个碳原子的烷烃和环氧化烯烃羟基化能力以及对应于细胞色素P450 BM-3的V78A，H236Q和E252G的氨基酸取代的那些变体。优选的细胞色素P450变体还包括在包含共溶剂如DMSO和THF的溶液中具有改善的羟基化活性的那些，并且具有对应于细胞色素P450 BM-3的T235A，R471A，E494K和S1024E的氨基酸取代。

4. 发明授权

US08076114B2 Cytochrome P450 oxygenases 有权
标题翻译：细胞色素P450加氧酶
公开(公告)号：US08076114B2
公开(公告)日：2011-12-13
申请号：US12754216
申请日：2010-04-05
申请人： Edgardo T Farinas , Frances H. Arnold , Ulrich Schwaneberg , Anton Glieder
发明人： Edgardo T Farinas , Frances H. Arnold , Ulrich Schwaneberg , Anton Glieder
IPC分类号： C12N9/02 , C12N15/00 , C12N1/20 , C12P21/04 , C07H21/04 , C12Q1/26
CPC分类号： C12N9/0071 , C12P7/02 , C12P7/04 , C12P7/16 , C12P17/02 , Y02E50/10 , Y02P20/52
摘要： Nucleic acids encoding cytochrome P450 variants are provided. The cytochrome P450 variants of have a higher alkane-oxidation capability, alkene-oxidation capability, and/or a higher organic-solvent resistance than the corresponding wild-type or parent cytochrome P450 enzyme. A preferred wild-type cytochrome P450 is cytochrome P450 BM-3. Preferred cytochrome P450 variants include those having an improved capability to hydroxylate alkanes and epoxidate alkenes comprising less than 8 carbons, and have amino acid substitutions corresponding to V78A, H236Q, and E252G of cytochrome P450 BM-3. Preferred cytochrome P450 variants also include those having an improved hydroxylation activity in solutions comprising co-solvents such as DMSO and THF, and have amino acid substitutions corresponding to T235A, R471A, E494K, and S1024E of cytochrome P450 BM-3.
摘要翻译：提供了编码细胞色素P450变体的核酸。细胞色素P450变体具有比相应的野生型或亲本细胞色素P450酶更高的烷烃氧化能力，烯烃氧化能力和/或较高的有机溶剂耐受性。优选的野生型细胞色素P450是细胞色素P450 BM-3。优选的细胞色素P450变体包括具有改善含有少于8个碳原子的烷烃和环氧化烯烃羟基化能力以及对应于细胞色素P450 BM-3的V78A，H236Q和E252G的氨基酸取代的那些变体。优选的细胞色素P450变体还包括在包含共溶剂如DMSO和THF的溶液中具有改善的羟基化活性的那些，并且具有对应于细胞色素P450 BM-3的T235A，R471A，E494K和S1024E的氨基酸取代。

5. 发明申请

US20100248327A1 CYTOCHROME P450 OXYGENASES 有权
标题翻译： CYTOCHROME P450氧化剂
公开(公告)号：US20100248327A1
公开(公告)日：2010-09-30
申请号：US12754216
申请日：2010-04-05
申请人： Edgardo T. Farinas , Frances H. Arnold , Ulrich Schwaneberg , Anton Glieder
发明人： Edgardo T. Farinas , Frances H. Arnold , Ulrich Schwaneberg , Anton Glieder
IPC分类号： C12N9/02 , C07H21/04
CPC分类号： C12N9/0071 , C12P7/02 , C12P7/04 , C12P7/16 , C12P17/02 , Y02E50/10 , Y02P20/52
摘要： Nucleic acids encoding cytochrome P450 variants are provided. The cytochrome P450 variants of have a higher alkane-oxidation capability, alkene-oxidation capability, and/or a higher organic-solvent resistance than the corresponding wild-type or parent cytochrome P450 enzyme. A preferred wild-type cytochrome P450 is cytochrome P450 BM-3. Preferred cytochrome P450 variants include those having an improved capability to hydroxylate alkanes and epoxidate alkenes comprising less than 8 carbons, and have amino acid substitutions corresponding to V78A, H236Q, and E252G of cytochrome P450 BM-3. Preferred cytochrome P450 variants also include those having an improved hydroxylation activity in solutions comprising co-solvents such as DMSO and THF, and have amino acid substitutions corresponding to T235A, R471A, E494K, and S1024E of cytochrome P450 BM-3.
摘要翻译：提供了编码细胞色素P450变体的核酸。细胞色素P450变体具有比相应的野生型或亲本细胞色素P450酶更高的烷烃氧化能力，烯烃氧化能力和/或较高的有机溶剂耐受性。优选的野生型细胞色素P450是细胞色素P450 BM-3。优选的细胞色素P450变体包括具有改善含有少于8个碳原子的烷烃和环氧化烯烃羟基化能力以及对应于细胞色素P450 BM-3的V78A，H236Q和E252G的氨基酸取代的那些变体。优选的细胞色素P450变体还包括在包含共溶剂如DMSO和THF的溶液中具有改善的羟基化活性的那些，并且具有对应于细胞色素P450 BM-3的T235A，R471A，E494K和S1024E的氨基酸取代。

6. 发明申请

US20080293928A1 Cytochrom P450 oxygenases 有权
标题翻译：细胞色素P450加氧酶
公开(公告)号：US20080293928A1
公开(公告)日：2008-11-27
申请号：US11800970
申请日：2007-05-07
申请人： Edgardo T. Farinas , Frances H. Arnold , Ulrich Schwaneberg , Anton Glieder
发明人： Edgardo T. Farinas , Frances H. Arnold , Ulrich Schwaneberg , Anton Glieder
IPC分类号： C07H21/04
CPC分类号： C12N9/0071 , C12P7/02 , C12P7/04 , C12P7/16 , C12P17/02 , Y02E50/10 , Y02P20/52
摘要： Nucleic acids encoding cytochrome P450 variants are provided. The cytochrome P450 variants of have a higher alkane-oxidation capability, alkene-oxidation capability, and/or a higher organic-solvent resistance than the corresponding wild-type or parent cytochrome P450 enzyme. A preferred wild-type cytochrome P450 is cytochrome P450 BM-3. Preferred cytochrome P450 variants include those having an improved capability to hydroxylate alkanes and epoxidate alkenes comprising less than 8 carbons, and have amino acid substitutions corresponding to V78A, H236Q, and E252G of cytochrome P450 BM-3. Preferred cytochrome P450 variants also include those having an improved hydroxylation activity in solutions comprising co-solvents such as DMSO and THF, and have amino acid substitutions corresponding to T235A, R471A, E494K, and S1024E of cytochrome P450 BM-3.
摘要翻译：提供了编码细胞色素P450变体的核酸。细胞色素P450变体具有比相应的野生型或亲本细胞色素P450酶更高的烷烃氧化能力，烯烃氧化能力和/或较高的有机溶剂耐受性。优选的野生型细胞色素P450是细胞色素P450 BM-3。优选的细胞色素P450变体包括具有改善含有少于8个碳原子的烷烃和环氧化烯烃羟基化能力以及对应于细胞色素P450 BM-3的V78A，H236Q和E252G的氨基酸取代的那些变体。优选的细胞色素P450变体还包括在包含共溶剂如DMSO和THF的溶液中具有改善的羟基化活性的那些，并且具有对应于细胞色素P450 BM-3的T235A，R471A，E494K和S1024E的氨基酸取代。

7. 发明授权

US08367386B2 Cytochrome P450 oxygenases 有权
公开(公告)号：US08367386B2
公开(公告)日：2013-02-05
申请号：US13323398
申请日：2011-12-12
申请人： Edgardo Farinas , Frances H. Arnold , Ulrich Schwaneberg , Anton Glieder
发明人： Edgardo Farinas , Frances H. Arnold , Ulrich Schwaneberg , Anton Glieder
IPC分类号： C12N9/02 , C12N1/20 , C12N15/00 , C12P21/04 , C12P21/06 , C07H21/04 , C12Q1/26
CPC分类号： C12N9/0071 , C12P7/02 , C12P7/04 , C12P7/16 , C12P17/02 , Y02E50/10 , Y02P20/52
摘要： Nucleic acids encoding cytochrome P450 variants are provided. The cytochrome P450 variants of have a higher alkane-oxidation capability, alkene-oxidation capability, and/or a higher organic-solvent resistance than the corresponding wild-type or parent cytochrome P450 enzyme. A preferred wild-type cytochrome P450 is cytochrome P450 BM-3. Preferred cytochrome P450 variants include those having an improved capability to hydroxylate alkanes and epoxidate alkenes comprising less than 8 carbons, and have amino acid substitutions corresponding to V78A, H236Q, and E252G of cytochrome P450 BM-3. Preferred cytochrome P450 variants also include those having an improved hydroxylation activity in solutions comprising co-solvents such as DMSO and THF, and have amino acid substitutions corresponding to T235A, R471A, E494K, and S1024E of cytochrome P450 BM-3.

8. 发明申请

US20100143986A1 R-HNL RANDOM VARIANTS AND THEIR USE FOR PREPARING OPTICALLY PURE, STERICALLY HINDERED CYANOHYDRINS 有权
标题翻译： R-HNL随机变量及其用于制备光学纯的，异味的氰基氰
公开(公告)号：US20100143986A1
公开(公告)日：2010-06-10
申请号：US12519095
申请日：2007-12-11
申请人： Richard Gaisberger , Anton Glieder , Zhibin Liu , Beate Pscheidt
发明人： Richard Gaisberger , Anton Glieder , Zhibin Liu , Beate Pscheidt
IPC分类号： C12P13/00 , C12N9/88
CPC分类号： C12N9/88 , C12P13/004 , C12P41/001
摘要： The invention relates to R-hydroxynitrile lyases with improved substrate acceptance, increased activity and increased selectivity, obtainable by introducing random mutations with the aid of random mutagenesis and/or saturation mutagenesis techniques, identifying by means of screening or selection and, where appropriate, subsequently combining advantageous mutations, and to the use thereof.
摘要翻译：本发明涉及具有改进的底物接受性，增加的活性和增加的选择性的R-羟基腈裂解酶，其可通过借助于随机诱变和/或饱和诱变技术引入随机突变而获得，通过筛选或选择鉴定，并且在适当的情况下随后结合有利的突变及其用途。

9. 发明申请

US20080153126A1 Mutant Aox1 Promoters 有权
标题翻译：突变型Aox1启动子
公开(公告)号：US20080153126A1
公开(公告)日：2008-06-26
申请号：US11817005
申请日：2006-02-23
申请人： Franz Hartner , Anton Glieder
发明人： Franz Hartner , Anton Glieder
IPC分类号： C12Q1/04 , C07H21/04 , C12N15/11 , C12N15/00 , C12N1/00 , C12P21/04
CPC分类号： C12N15/815 , C12Y101/03013
摘要： A mutant Pichia pastoris alcohol oxidase 1 (AOX1) promoter of the wild type Pichia pastoris AOX1 promoter (SEQ ID No. 1) comprising at least one mutation selected from the group consisting of: a) a transcription factor binding site (TFBS), b) nucleotides 170 to 235 (−784 to −719), nucleotides 170 to 191 (−784 to −763), nucleotides 192 to 213 (−762 to −741), nucleotides 192 to 210 (−762 to −744), nucleotides 207 to 209 (−747 to −745), nucleotides 214 to 235 (−740 to −719), nucleotides 304 to 350 (−650 to −604), nucleotides 364 to 393 (−590 to −561), nucleotides 434 to 508 (−520 to −446), nucleotides 509 to 551 (−445 to −403), nucleotides 552 to 560 (−402 to −394), nucleotides 585 to 617 (−369 to −337), nucleotides 621 to 660 (−333 to −294), nucleotides 625 to 683 (−329 to −271), nucleotides 736 to 741 (−218 to −213), nucleotides 737 to 738 (−217 to −216), nucleotides 726 to 755 (−228 to −199), nucleotides 784 to 800 (−170 to −154) or nucleotides 823 to 861 (−131 to −93) of Seq ID No. 1, and combinations thereof.
摘要翻译：包含选自以下的至少一个突变的野生型巴斯德毕赤酵母AOX1启动子（SEQ ID No.1）的突变型巴斯德毕赤酵母醇氧化酶1（AOX1）启动子：a）转录因子结合位点（TFBS），b ）核苷酸170至235（-784至-719），核苷酸170至191（-784至-763），核苷酸192至213（-762至-741），核苷酸192至210（-762至-744），核苷酸 207至209（-747至-745），核苷酸214至235（-740至-719），核苷酸304至350（-650至-604），核苷酸364至393（-590至-561），核苷酸434至 508（-520至-446），核苷酸509至551（-445至-403），核苷酸552至560（-402至-394），核苷酸585至617（-369至-337），核苷酸621至660（ -333至-294），核苷酸625至683（-329至-271），核苷酸736至741（-218至-213），核苷酸737至738（-217至-216），核苷酸726至755（-228 至-199），Seq ID No.1的核苷酸784至800（-170至-154）或核苷酸823至861（-131至-93）及其组合。

10. 发明申请

US20100196913A1 Mutant AOX1 Promoters 有权
标题翻译：突变型AOX1启动子
公开(公告)号：US20100196913A1
公开(公告)日：2010-08-05
申请号：US12759763
申请日：2010-04-14
申请人： Franz Hartner , Anton Glieder
发明人： Franz Hartner , Anton Glieder
IPC分类号： C12Q1/68 , C07H21/04 , C12N15/63 , C12N1/19
CPC分类号： C12N15/815 , C12Y101/03013
摘要： A mutant Pichia pastoris alcohol oxidase 1 (AOX1) promoter of the wild type Pichia pastoris AOX1 promoter (SEQ ID No. 1) comprising at least one mutation selected from the group consisting of: a) a transcription factor binding site (TFBS), b) nucleotides 170 to 235 (−784 to −719), nucleotides 170 to 191 (−784 to −763), nucleotides 192 to 213 (−762 to −741), nucleotides 192 to 210 (−762 to −744), nucleotides 207 to 209 (−747 to −745), nucleotides 214 to 235 (−740 to −719), nucleotides 304 to 350 (−650 to −604), nucleotides 364 to 393 (−590 to −561), nucleotides 434 to 508 (−520 to −446), nucleotides 509 to 551 (−445 to −403), nucleotides 552 to 560 (−402 to −394), nucleotides 585 to 617 (−369 to −337), nucleotides 621 to 660 (−333 to −294), nucleotides 625 to 683 (−329 to −271), nucleotides 736 to 741 (−218 to −213), nucleotides 737 to 738 (−217 to −216), nucleotides 726 to 755 (−228 to −199), nucleotides 784 to 800 (−170 to −154) or nucleotides 823 to 861 (−131 to −93) of Seq ID No. 1, and combinations thereof.
摘要翻译：一种突变巴斯德毕赤酵母醇氧化酶1（AOX1）野生型毕赤酵母AOX1启动子的启动子（序列号1），其包括选自由以下组成的组中选择的至少一个突变：a）转录因子结合位点（TFBS），B ）核苷酸170到235（-784到-719），核苷酸170至191（-784到-763），核苷酸192至213（-762到-741），核苷酸192至210（-762到-744），核苷酸 207至209（-747到-745），核苷酸214至235（-740到-719），核苷酸304至350（-650到-604），核苷酸364至393（-590到-561），核苷酸434到 508（-520到-446），核苷酸509至551（-445到-403），核苷酸552至560（-402到-394），核苷酸585至617（-369到-337），核苷酸621至660（ -333到-294），核苷酸625至683（-329到-271），核苷酸736至741（-218到-213），核苷酸737至738（-217到-216），核苷酸726至755（-228 至-199），核苷酸784至800（ 170至-154）或核苷酸SEQ ID No.1中，和它们的组合的823至861（-131到-93）。

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