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1. 发明授权

US09909110B2 Compositions and methods comprising sequences having meganuclease activity 有权
公开(公告)号：US09909110B2
公开(公告)日：2018-03-06
申请号：US15289993
申请日：2016-10-11
申请人： E I DU PONT DE NEMOURS AND COMPANY , PIONEER HI-BRED INTERNATIONAL, INC.
发明人： Ericka Bermudez , Andrew Mark Cigan , James English , Saverio Carl Falco , Huirong Gao , Lu Liu , Zhan-Bin Liu , Azalea Ong , Sergei Svitashev , Joshua K Young
IPC分类号： C12N15/82 , C12N9/16 , C12N9/22 , C12N15/81
CPC分类号： C12N9/22 , C12N9/16 , C12N15/81 , C12N15/8213 , C12Y301/00
摘要： Compositions and methods comprising polynucleotides and polypeptides having meganuclease activity are provided. Further provided are nucleic acid constructs, yeast, plants, plant cells, explants, seeds and grain having the meganuclease sequences. Various methods of employing the meganuclease sequences are provided. Such methods include, for example, methods for producing a meganuclease with increased activity at a wide range of temperatures, methods for producing a yeast, plant, plant cell, explant or seed comprising a meganuclease with increased activity.

2. 发明授权

US09499827B2 Compositions and methods comprising sequences having meganuclease activity 有权
标题翻译：包含具有大范围核酸酶活性的序列的组合物和方法
公开(公告)号：US09499827B2
公开(公告)日：2016-11-22
申请号：US13886317
申请日：2013-05-03
申请人： E I DU PONT DE NEMOURS AND COMPANY , PIONEER HI BRED INTERNATIONAL INC
发明人： Ericka Bermudez , Andrew Mark Cigan , James English , Saverio Carl Falco , Huirong Gao , Lu Liu , Zhan-Bin Liu , Azalea Ong , Sergei Svitashev , Joshua K Young
IPC分类号： C12N15/82 , C12N5/14 , C12N9/22 , C12N9/16 , C12N15/81
CPC分类号： C12N9/22 , C12N9/16 , C12N15/81 , C12N15/8213 , C12Y301/00
摘要： Compositions and methods comprising polynucleotides and polypeptides having meganuclease activity are provided. Further provided are nucleic acid constructs, yeast, plants, plant cells, explants, seeds and grain having the meganuclease sequences. Various methods of employing the meganuclease sequences are provided. Such methods include, for example, methods for producing a meganuclease with increased activity at a wide range of temperatures, methods for producing a yeast, plant, plant cell, explant or seed comprising a meganuclease with increased activity.
摘要翻译：提供了包含具有大范围核酸酶活性的多核苷酸和多肽的组合物和方法。还提供了具有大范围核酸酶序列的核酸构建体，酵母，植物，植物细胞，外植体，种子和颗粒。提供了使用大范围核酸酶序列的各种方法。这样的方法包括例如用于生产在宽温度范围内具有增加的活性的大范围核酸酶的方法，用于生产酵母，植物，植物细胞，外植体或包含具有增加的活性的大范围核酸酶的种子的方法。

3. 发明申请

US20150184173A1 COMPOSITIONS AND METHODS COMPRISING SEQUENCES HAVING MEGANUCLEASE ACTIVITY 审中-公开
标题翻译：组合物和包含游离基活性的序列的方法
公开(公告)号：US20150184173A1
公开(公告)日：2015-07-02
申请号：US14398592
申请日：2013-05-01
申请人： E I DU PONT DE NEMOURS AND COMPANY , PIONEER HI BRED INTERNATIONAL INC.
发明人： Ericka Bermudez , Andrew Mark Cigan , James English , Saverio Carl Falco , Huirong Gao , Lu Liu , Zhan-bin Liu , Azalea Ong , Sergei Svitashev , Joshua K. Young
IPC分类号： C12N15/82 , C12N15/81
CPC分类号： C12N9/22 , C12N9/16 , C12N15/81 , C12N15/8213 , C12Y301/00
摘要： Compositions and methods comprising polynucleotides and polypeptides having meganuclease activity are provided. Further provided are nucleic acid constructs, yeast, plants, plant cells, explants, seeds and grain having the meganuclease sequences. Various methods of employing the meganuclease sequences are provided. Such methods include, for example, methods for producing a meganuclease with increased activity at a wide range of temperatures, methods for producing a yeast, plant, plant cell, explant or seed comprising a meganuclease with increased activity.
摘要翻译：提供了包含具有大范围核酸酶活性的多核苷酸和多肽的组合物和方法。还提供了具有大范围核酸酶序列的核酸构建体，酵母，植物，植物细胞，外植体，种子和颗粒。提供了使用大范围核酸酶序列的各种方法。这样的方法包括例如用于生产在宽温度范围内具有增加的活性的大范围核酸酶的方法，用于生产酵母，植物，植物细胞，外植体或包含具有增加的活性的大范围核酸酶的种子的方法。

4. 发明申请

US20160348124A1 NOVEL DGAT GENES FOR INCREASED SEED STORAGE LIPID PRODUCTION AND ALTERED FATTY ACID PROFILES IN OILSEED PLANTS 审中-公开
公开(公告)号：US20160348124A1
公开(公告)日：2016-12-01
申请号：US15239069
申请日：2016-08-17
申请人： E I DU PONT DE NEMOURS AND COMPANY
发明人： KEITH ROESLER , Ericka Bermudez , Howard Glenn Damude , Changjiang Li , Knut Meyer , Bo Shen , Mitchell C. Tarczynski
IPC分类号： C12N15/82 , A23K10/30 , A23L11/00 , C12N9/10
CPC分类号： C12N15/8247 , A23K10/30 , A23L11/00 , A23V2002/00 , C12N9/1029 , C12Y203/0102
摘要： Transgenic oilseeds having increased total fatty acid content of at least 10% and altered fatty acid profiles when compared to the total fatty acid content of null segregant oilseeds are described. Novel DGAT genes are used to achieve the increase in seed storage lipids.

5. 发明授权

US10017777B2 DGAT genes for increased seed storage lipid production and altered fatty acid profiles in oilseed plants 有权
公开(公告)号：US10017777B2
公开(公告)日：2018-07-10
申请号：US15239069
申请日：2016-08-17
申请人： E I DU PONT DE NEMOURS AND COMPANY
发明人： Keith Roesler , Ericka Bermudez , Howard Glenn Damude , Changjiang Li , Knut Meyer , Bo Shen , Mitchell C Tarczynski
IPC分类号： C12N15/82 , C12N9/10 , A23K10/30 , A23L11/00
CPC分类号： C12N15/8247 , A23K10/30 , A23L11/00 , A23V2002/00 , C12N9/1029 , C12Y203/0102
摘要： Transgenic oilseeds having increased total fatty acid content of at least 10% and altered fatty acid profiles when compared to the total fatty acid content of null segregant oilseeds are described. Novel DGAT genes are used to achieve the increase in seed storage lipids.

6. 发明授权

US09447386B2 DGAT genes for increased seed storage lipid production and altered fatty acid profiles in oilseed plants 有权
公开(公告)号：US09447386B2
公开(公告)日：2016-09-20
申请号：US13888882
申请日：2013-05-07
申请人： E I DU PONT DE NEMOURS AND COMPANY
发明人： Howard Glenn Damude , Ericka Bermudez , Changjiang Li , Knut Meyer , Keith Roesler , Bo Shen , Mitchell C Tarczynski
IPC分类号： C12N9/10 , C12N15/82 , A01H1/06
CPC分类号： C12N15/8247 , A23K10/30 , A23L11/00 , A23V2002/00 , C12N9/1029 , C12Y203/0102
摘要： Transgenic oilseeds having increased total fatty acid content of at least 10% and altered fatty acid profiles when compared to the total fatty acid content of null segregant oilseeds are described. Novel DGAT genes are used to achieve the increase in seed storage lipids.

7. 发明申请

US20180291391A1 NOVEL DGAT GENES FOR INCREASED SEED STORAGE LIPID PRODUCTION AND ALTERED FATTY ACID PROFILES IN OILSEED PLANTS 审中-公开
公开(公告)号：US20180291391A1
公开(公告)日：2018-10-11
申请号：US16005018
申请日：2018-06-11
申请人： E. I. DU PONT DE NEMOURS AND COMPANY
发明人： Keith Roesler , Ericka Bermudez , Howard Glenn Damude , Changjiang Li , Knut Meyer , Bo Shen , Mitchell C. Tarczynski
IPC分类号： C12N15/82 , C12N9/10 , A23K10/30 , A23L11/00
摘要： Transgenic oilseeds having increased total fatty acid content of at least 10% and altered fatty acid profiles when compared to the total fatty acid content of null segregant oilseeds are described. Novel DGAT genes are used to achieve the increase in seed storage lipids.

8. 发明授权

US10329578B2 Glyphosate-N-acetyltransferase (GLYAT) sequences and methods of use 有权
公开(公告)号：US10329578B2
公开(公告)日：2019-06-25
申请号：US15029783
申请日：2014-10-14
申请人： PIONEER HI-BRED INTERNATIONAL, INC.
发明人： Ericka Bermudez , Linda A. Castle , Kevin E. McBride , Daniel Siehl
IPC分类号： C12N15/82 , C12N9/10
摘要： Compositions and methods comprising polynucleotides and polypeptides having glyphosate-N-acetyltransferase (GLYAT) activity are provided. In specific embodiments, the sequence has an improved property, such as, but not limited to, an improved specificity for glyphosate when compared to an appropriate control resulting in decreased off target acetylation of, e.g. an amino acid such as aspartate. Further provided are nucleic acid constructs, plants, plant cells, explants, seeds and grain having the GLYAT sequences. Various methods of employing the GLYAT sequences are provided. Such methods include methods for producing a glyphosate tolerant plant, plant cell, explant or seed and methods of controlling weeds in a field containing a crop employing the plants and/or seeds disclosed herein.

9. 发明申请

US20160257971A1 GLYPHOSATE-N-ACETYLTRANSFERASE (GLYAT) SEQUENCES AND METHODS OF USE 审中-公开
标题翻译：谷氨酸-N-乙酰胆碱酯酶（GLYAT）序列及其使用方法
公开(公告)号：US20160257971A1
公开(公告)日：2016-09-08
申请号：US15029783
申请日：2014-10-14
申请人： PIONEER HI-BRED INTERNATIONAL, INC.
发明人： Ericka Bermudez , Linda A. Castle , Kevin E. McBride , Daniel Siehl
IPC分类号： C12N15/82 , C12N9/10
CPC分类号： C12N15/8275 , C12N9/1029
摘要： Compositions and methods comprising polynucleotides and polypeptides having glyphosate-N-acetyltransferase (GLYAT) activity are provided. In specific embodiments, the sequence has an improved property, such as, but not limited to, an improved specificity for glyphosate when compared to an appropriate control resulting in decreased off target acetylation of, e.g. an amino acid such as aspartate. Further provided are nucleic acid constructs, plants, plant cells, explants, seeds and grain having the GLYAT sequences. Various methods of employing the GLYAT sequences are provided. Such methods include methods for producing a glyphosate tolerant plant, plant cell, explant or seed and methods of controlling weeds in a field containing a crop employing the plants and/or seeds disclosed herein.
摘要翻译：提供了包含具有草甘膦-N-乙酰基转移酶（GLYAT）活性的多核苷酸和多肽的组合物和方法。在具体实施方案中，当与适当的对照相比时，该序列具有改进的性质，例如但不限于对草甘膦的改善的特异性，导致减少脱乙酰化的脱乙酰化。氨基酸，如天冬氨酸。还提供了具有GLYAT序列的核酸构建体，植物，植物细胞，外植体，种子和谷物。提供了使用GLYAT序列的各种方法。这样的方法包括用于产生耐草甘膦植物，植物细胞，外植体或种子的方法以及在含有本文公开的植物和/或种子的作物的田地中控制杂草的方法。

10. 发明申请

US20130225799A1 NOVEL DGAT GENES FOR INCREASED SEED STORAGE LIPID PRODUCTION AND ALTERED FATTY ACID PROFILES IN OILSEED PLANTS 审中-公开
公开(公告)号：US20130225799A1
公开(公告)日：2013-08-29
申请号：US13888882
申请日：2013-05-07
申请人： Pioneer Hi-Bred International, Inc. , E.I. du Pont de Nemours And Company
发明人： KEITH ROESLER , Knut Meyer , Howard Glenn Damude , Bo Shen , Changjiang Li , Ericka Bermudez , Mitchell C. Tarczynski
IPC分类号： C12N9/10
CPC分类号： C12N15/8247 , A23K10/30 , A23L11/00 , A23V2002/00 , C12N9/1029 , C12Y203/0102
摘要： Transgenic oilseeds having increased total fatty acid content of at least 10% and altered fatty acid profiles when compared to the total fatty acid content of null segregant oilseeds are described. Novel DGAT genes are used to achieve the increase in seed storage lipids.

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