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    • 7. 发明授权
    • Methods for detecting differentially expressed genes
    • 检测差异表达基因的方法
    • US06218114B1
    • 2001-04-17
    • US09049569
    • 1998-03-27
    • Konan PeckJeremy J. W. ChenPan-Chyr YangReen WuFu ChangYi-Wen ChuCheng-Wen Wu
    • Konan PeckJeremy J. W. ChenPan-Chyr YangReen WuFu ChangYi-Wen ChuCheng-Wen Wu
    • C12Q168
    • C12Q1/6809
    • The invention relates to a method of detecting a differentially expressed gene in a first sample of nucleic acids representing a first population of RNA transcripts and a second sample of nucleic acids representing a second population of RNA transcripts. The nucleic acids in the samples are labled with a member of specific binding pair, and the labeled nucleic acids in each sample are then hybridized to an excess of copies of a gene-specific sequence. The hybridized nucleic acids in each sample are further labeled by binding a second member of the specific binding pair to the first member, in which the second member has an activity to convert a chromogenic substrate into a chromogen. As a result of contacting the second member with the chromogenic substrate, the chromogenic substrate is converted into the chromogen. A difference in the amounts of chromogen produced from assaying the two samples indicate that the gene-specific sequence is differentially expressed in the samples.
    • 本发明涉及检测代表第一RNA转录物群体的核酸的第一样本中的差异表达基因的方法和表示第二RNA转录物群体的第二核酸样品。 样品中的核酸用特异性结合对的成员进行标记,然后将每个样品中的标记的核酸与过量的基因特异性序列的拷贝杂交。 通过将特异性结合对的第二成员与第一成员结合来进一步标记每个样品中的杂交核酸,其中第二成员具有将显色底物转化为色原体的活性。 作为使第二构件与显色底物接触的结果,将显色底物转化为色原体。 通过测定两个样品产生的色原体的量的差异表明基因特异性序列在样品中差异表达。