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    • 4. 发明申请
    • Methods and reagents for assaying molecular interactions
    • 用于测定分子相互作用的方法和试剂
    • US20060099645A1
    • 2006-05-11
    • US10526733
    • 2003-09-05
    • Jeff WranaMiriam Barrios-Rodiles
    • Jeff WranaMiriam Barrios-Rodiles
    • G01N33/53
    • C40B30/04C12N15/1055C12Q1/66G01N33/5008G01N33/502G01N33/6845
    • The invention relates to methods and reagents for screening, identifying, and/or quantifying molecular interactions. In particular, the invention provides a method for identifying protein-protein interactions comprising prey proteins interacting with bait proteins comprising: (a) introducing one or more prey protein in cells, wherein a prey protein is labelled with an epitope tag permitting separation of the prey protein from other proteins in the cells; (b) introducing one or more bait protein in the cells, wherein a bait protein is labelled with a detectable substance permitting detection of protein-protein interactions comprising a prey protein and the bait protein; and (c) assaying for protein-protein interactions comprising a prey protein and bait protein by detecting the detectable substance.
    • 本发明涉及用于筛选,鉴定和/或定量分子相互作用的方法和试剂。 特别地,本发明提供了一种用于鉴定蛋白质 - 蛋白质相互作用的方法,其包括与诱饵蛋白相互作用的猎物蛋白质,其包括:(a)在细胞中引入一种或多种猎物蛋白质,其中猎物蛋白用允许猎物分离的表位标签 细胞中其他蛋白质的蛋白质; (b)在细胞中引入一种或多种诱饵蛋白质,其中诱饵蛋白质用可检测物质进行标记,其允许检测包含猎物蛋白质和诱饵蛋白质的蛋白质 - 蛋白质相互作用; 和(c)通过检测可检测物质来测定包含猎物蛋白质和诱饵蛋白质的蛋白质 - 蛋白质相互作用。