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    • 5. 发明授权
    • Method and apparatus for co-culturing cells
    • 共培养细胞的方法和装置
    • US5763275A
    • 1998-06-09
    • US676311
    • 1996-09-09
    • Hans-Otto NagelsDieter SchroderEckart Kopowski
    • Hans-Otto NagelsDieter SchroderEckart Kopowski
    • A61K48/00C12M3/04C12M3/06C12N5/00C12N5/10
    • C12M23/08C12M29/04C12M35/08
    • The method relates to the co-culturing of two cell cultures in two cell culture chambers such that the cellular products produced by the co-cultured cells are exchanged between the two cell culture chambers. The apparatus for culturing the cells contains two cell culture chambers separated by a microfiltration membrane which is permeable to medium, gases, and cellular products, and is impermeable to cells. A culture medium supply chamber is adjacent to and separated from the first cell culture chamber by a dialysis membrane which is permeable to medium and gases. The exterior portion of the second cell culture contains a gas-exchange membrane which allows gas exchange between the apparatus and the environment. The apparatus can be in a modular form where the first and second cell culture chambers form one module which is detachable from the culture medium supply chamber which forms the second module. The method and apparatus allows the exchange of media, gases, and cellular products between the two cell cultures while preventing direct contact of the two cell cultures.
    • PCT No.PCT / EP95 / 04190 Sec。 371日期1996年9月9日 102(e)1996年9月9日PCT PCT 1995年10月25日PCT公布。 WO96 / 16161 PCT出版物 日期1996年5月30日该方法涉及在两个细胞培养室中共培养两种细胞培养物,使得由共培养细胞产生的细胞产物在两个细胞培养室之间交换。 用于培养细胞的装置包含两个细胞培养室,该细胞培养室由可渗透培养基,气体和细胞产物的微滤膜分离,并且对细胞是不可渗透的。 培养基供应室通过可渗透中等和气体的透析膜与第一细胞培养室相邻并分离。 第二细胞培养物的外部部分包含允许装置和环境之间的气体交换的气体交换膜。 该装置可以是模块化形式,其中第一和第二细胞培养室形成一个可从形成第二模块的培养基供应室拆卸的模块。 该方法和装置允许在两种细胞培养物之间交换介质,气体和细胞产物,同时防止两种细胞培养物的直接接触。
    • 6. 发明授权
    • Culture vessel for cell cultures on a carrier
    • 载体上细胞培养物的培养容器
    • US5702945A
    • 1997-12-30
    • US595351
    • 1996-02-01
    • Hans-Otto NagelsDieter SchroderEckart Kopowski
    • Hans-Otto NagelsDieter SchroderEckart Kopowski
    • A61F2/06A61L27/60C12M3/06C12N5/00C12N5/08
    • C12N5/0068A61F2/062A61L27/60C12M23/24C12M23/48C12M25/02C12M29/04C12N2533/40
    • The purpose of the present invention is to attain optimal gas exchange, nutrient supply and disposal of metabolic waste products without contamination during high-density cell cultivation of adherent cells. The culture vessel of the present invention provides methods and apparatus for the cultivation of cells on a three-dimensional carrier in a closed cell culture chamber, the supply with oxygen taking place across a gas-permeable gas exchange membrane bordering the cell culture chamber and the supply with nutrients taking place across a dialysis membrane bordering the cell culture chamber, across which nutrients are transported and across which metabolic products are removed, without the danger of contaminating the cells during contact with the tools necessary for manually separating the cells being cultivated from the nutrient medium. The cell culture chamber of the present invention features a vessel that is impermeable to fluids on all sides except for the boundary formed by the dialysis membrane. This makes it possible to move the cell culture intensively within a fluid nutrient supply medium. It is neither necessary to open the cell culture chamber during cultivation to introduce new nutrients, nor is it necessary for the design to include components which reach into the cell culture chamber, and form an additional source of contamination. The gas exchange membrane is detachable to allow removal of the cultivated cells in a uniform layer on the carrier, and the carrier is bio-degradable to eliminate the need to remove the cells from the carrier prior to use, such as in the transplantation of skin for example.
    • 本发明的目的是在贴壁细胞的高密度细胞培养过程中达到最佳的气体交换,代谢废物的营养供应和处置而不受污染。 本发明的培养容器提供了在闭孔细胞培养室中在三维载体上培养细胞的方法和装置,在与细胞培养室接壤的透气性气体交换膜和 提供与细胞培养室相邻的透析膜上的营养物质,跨越其间运输营养物质并除去代谢产物,而不会在与手工分离培养的细胞所需的工具接触期间污染细胞的危险 营养培养基。 本发明的细胞培养室具有除透析膜形成的边界以外的所有侧面的流体不可渗透的血管。 这使得可以在流体营养供应介质内集中移动细胞培养物。 在培养期间不需要打开细胞培养室以引入新的营养物,也不需要将设计包括进入细胞培养室的组分,并形成另外的污染源。 气体交换膜是可拆卸的,以允许移除载体上均匀层中的培养细胞,并且载体是生物可降解的,以消除在使用前从载体中除去细胞的需要,例如在皮肤移植中 例如。