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1. 发明授权

US5910486A Methods for modulating protein function in cells using, intracellular antibody homologues 失效
标题翻译：使用细胞内抗体同系物调节细胞中蛋白质功能的方法
公开(公告)号：US5910486A
公开(公告)日：1999-06-08
申请号：US468252
申请日：1995-06-06
申请人： David T. Curiel , Jessy Deshane
发明人： David T. Curiel , Jessy Deshane
IPC分类号： A61K48/00 , C07K16/32
CPC分类号： C07K16/32 , A61K2039/505 , A61K48/00 , C07K2317/622 , C12N2799/022
摘要： Methods and compositions for modulating protein function in a cell involving intracellular expression of an antibody homologue that binds to the protein within the cell are disclosed. In a preferred embodiment, an antibody homologue, such as a single chain Fv (sFv) fragment, is expressed within an intracellular compartment of a cell, such as the endoplasmic reticulum (ER), to inhibit cell surface expression of a membrane protein. Preferably, the cell is a malignant mammalian cell and the protein is a cell surface receptor oncoprotein, such as c-erbB2. Intracellular binding of the antibody homologue to the receptor oncoprotein inhibits its surface expression and, moreover, inhibits cell proliferation and cell survival. Isolated nucleic acid molecules encoding anti-c-erbB2 antibody homologues, as well as recombinant expression vectors and host cells incorporating these nucleic acid molecules, are also disclosed.
摘要翻译：公开了涉及在细胞内与蛋白质结合的抗体同系物的细胞内表达的细胞中调节蛋白质功能的方法和组合物。在优选的实施方案中，抗体同系物，例如单链Fv（sFv）片段在细胞的细胞内隔室（例如内质网（ER））内表达，以抑制膜蛋白的细胞表面表达。优选地，细胞是恶性哺乳动物细胞，并且蛋白质是细胞表面受体癌蛋白，例如c-erbB2。抗体同源物与受体癌蛋白的细胞内结合抑制其表面表达，此外抑制细胞增殖和细胞存活。还公开了编码抗c-erbB2抗体同系物的分离的核酸分子，以及重组表达载体和掺入这些核酸分子的宿主细胞。

2. 发明授权

US06028059A Methods for modulating protein function in cells using intracellular antibody homologues 失效
标题翻译：使用细胞内抗体同系物调节细胞中蛋白质功能的方法
公开(公告)号：US06028059A
公开(公告)日：2000-02-22
申请号：US668706
申请日：1996-06-24
申请人： David T. Curiel , Jessy Deshane
发明人： David T. Curiel , Jessy Deshane
IPC分类号： A61K48/00 , C07K16/32
CPC分类号： C07K16/32 , A61K2039/505 , A61K48/00 , C07K2317/622 , C12N2799/022
摘要： Methods and compositions for modulating protein function in a cell involving intracellular expression of an antibody homologue that binds to the protein within the cell are disclosed. In a preferred embodiment, an antibody homologue, such as a single chain Fv (sFv) fragment, is expressed within an intracellular compartment of a cell, such as the endoplasmic reticulum (ER), to inhibit cell surface expression of a membrane protein. Preferably, the cell is a malignant mammalian cell and the protein is a cell surface receptor oncoprotein, such as erbB2. Intracellular binding of the antibody homologue to the receptor oncoprotein inhibits its surface expression and, moreover, inhibits cell proliferation and cell survival. Isolated nucleic acid molecules encoding anti-c-erbB2 antibody homologues, as well as recombinant expression ventors and host cells incorporating these nucleic acid molecules, are also disclosed.
摘要翻译：公开了涉及在细胞内与蛋白质结合的抗体同系物的细胞内表达的细胞中调节蛋白质功能的方法和组合物。在优选的实施方案中，抗体同系物，例如单链Fv（sFv）片段在细胞的细胞内隔室（例如内质网（ER））内表达，以抑制膜蛋白的细胞表面表达。优选地，细胞是恶性哺乳动物细胞，并且蛋白质是细胞表面受体癌蛋白，例如erbB2。抗体同源物与受体癌蛋白的细胞内结合抑制其表面表达，此外抑制细胞增殖和细胞存活。还公开了编码抗c-erbB2抗体同系物的分离的核酸分子以及掺入这些核酸分子的重组表达细胞和宿主细胞。

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